Objective To discuss the influence of the holistic nursing careng on clinical value in patients with advanced pancreatic cancer.Methods 70 patients with pancreatic cancer were randomly divided into the observation group and the control group,with 35 cases in each group.The control group was given routine nursing measures.The observation group received the holistic nursing careng on the basis of routine nursing measures.The hospitalization time,satisfaction degree,treatment compliance and mentality were observed.Results The SAS,SDS scores of the observation group after nursing were (56.87 ±6.01)points and (61.79 ±6.17)points,which were significantly lower than (39.11 ±5.68)points and (40.82 ±5.31)points of before nursing(t =9.136,8.561,all P <0.05).The hospi-talization time,exhaust time of the observation group were (32.9 ±6.6)days and (8.21 ±1.29)days,which were significantly lower than (45.1 ±10.2)days and (13.56 ±1.77)days of the control group(t =8.561,3.769,all P <0.05).The treatment satisfaction and treatment compliance in the observation group were also significantly better than the control group(χ2 =8.292,8.156,all P <0.05).Conclusion Hsystematic nursing intervention can significantly improve thepostoperative anxiety and depression,and improve the quality of life of patients with severe pneumonia.

Aim To investigate the effect of ginkgolide B on junctional proteins in ox-LDL-stimulated human umbilical vein endothelial cells ( HUVECs) . Methods After incubation with ginkgolide B ( 0 . 2 ,0 . 4 ,0 . 6 g · L-1 ) for 1 h, HUVECs were treated with ox-LDL (0. 1 g·L-1 ) for 4 h. The expressions of JAM-A and Cx43 were analyzed with Western blot and immunofluo-rescence. The effect of ginkgolide B on vascular per-meability was analyzed by Transwell experiments. Re-sults JAM-A and Cx43 expressions increased by 22%and 24% in ox-LDL-treated HUVECs, respectively. Whereas ginkgolide B significantly decreased JAM-A and Cx43 expressions. LY294002, a specific inhibitor of PI3K, suppressed JAM-A and Cx43 expressions in ox-LDL-stimulated cells. Ginkgolide B potently re-duced monocyte migration in ox-LDL-treated cells. Conclusion Ginkgolide B significantly suppresses JAM-A and Cx43 expressions, and reduces monocyte migration in ox-LDL-stimulated cells. This demon-strates that ginkgolide B can improve vascular permea-bility. The mechanism might be associated with the in-hibition of PI3K/Akt signaling pathway.

Objective To explore the influence of the dual tutorial teaching mode on the undergraduate
nursing students′ critical thinking in the clinical training. Methods Two hundred and sixteen full-time undergraduate nursing students who did internships in the second Affiliated Hospital of Wenzhou Medical University from September 1st, 2013 to September 1st, 2015 were divided into the experimental group ( n=111) and the control group ( n=105) using the random number table. The nursing students in the control group received the traditional post tutor teaching mode, while the nursing students in the experimental group received the dual tutorial system teaching mode in the clinical teaching. After the internship, nursing students in the two groups completed the general information questionnaire and the critical thinking disposition inventory.Results The scores of critical thinking ability, open-minded ability, analytical ability, systematic ability, critical thinking self-confidence, curiosity, cognitive maturity dimension of the nursing students in the experimental group were (280.32±29.83), (40.53±5.75), (43.08±5.32), (37.69±6.08), (40.16±5.60), (43.89±6.71), (38.53± 7.17) respectively, which were significantly higher than the scores gained by the nursing students in the control group (t=-7.587, -6.138, -8.555, -3.530, -6.727, -8.023, -3.097;P<0.05).In the control group, they were (210.63±25.25), (29.86±6.31), (26.61±5.71), (32.19±6.17), (28.76±5.82), (25.96±6.53), (32.99±7.41), respectively.Conclusions The dual tutorial teaching mode improves undergraduate nursing trainees′critical thinking ability including open-minded ability, analytical ability, systematic ability, critical thinking self-confidence, curiosity, cognitive maturity, which can be applied in the clinical teaching of nursing students.

Objective To study the effect of humanistic nursing intervention on the pre-operation emotion of patients with gallstone.Methods Totals of 84 patients with gallstone operation during the period from October 2012 to October 2013 were randomly divided into control group ( n=42 ) that received routine nursing and observation group ( n=42) that received humanistic nursing intervention.The SAS scores and SDS scores on admission and pre-operation in the two groups according to the self anxiety scale ( SAS) and the self depression scale (SDS), and the nursing satisfaction degree in the two groups were detected and compared.Results No significant difference was found in the scores of SAS and SDS between two groups on admission (P>0.05). Compared with control group, the SAS scores (33.7 ±10.0 vs 48.3 ±12.0),and SDS scores (42.0 ±13.0 vs 55.0 ±15.0) in observation group on the pre-operation were significantly reduced (t=3.54,3.67,respectively;P<0.05).And the nursing satisfaction degree in observation group was obviously higher than that in control group(95.2%vs 81.0%;χ2 =4.09,P<0.05).Conclusions For the patients with gallstone operation, the humanistic nursing intervention can significantly improve their pre-operative negative emotion, increase the nursing satisfaction, which is worthy of clinical promotion.

Objective:To investigate the efficacy and value of preemptive analgesia in older adult patients undergoing laparoscopic hepatectomy.Methods:A total of 150 older adult patients with liver cancer, who underwent laparoscopic hepatectomy at The 2 nd Affiliated Hospital of Wenzhou Medical University between July 2019 and April 2020, were randomly assigned to two groups: a control group ( n = 75) and an observation group ( n = 75) using the random number table method. The control group received postoperative analgesia with parecoxib sodium, while the observation group received preemptive analgesia with parecoxib sodium. A comparative analysis was conducted between the control and observation groups in terms of the Numerical Rating Scale (NRS) scores at 8, 12, and 24 hours postoperatively, postoperative recovery, length of hospital stay, and overall cost. Results:There were no statistically significant differences in operation time, dosage of Sufentanil 24 hours postoperatively, or the total and effective usage counts of patient-controlled intravenous analgesia with Sufentanil between the control and observation groups (both P > 0.05). However, the NRS scores of the observation group were significantly lower than those of the control group at 8, 12, and 24 hours postoperatively. Specifically, the NRS scores of the observation group were (4.38 ± 1.24) points, (3.41 ± 0.19) points, and (2.90 ± 0.17) points, respectively, while those of the control group were (5.24 ± 1.01) points, (4.65 ± 1.24) points, and (3.32 ± 1.00) points, respectively ( t = 4.66, 8.56, 3.59, all P < 0.001). Patients in the observation group exhibited significantly more frequent off-bed activities [(2.62 ± 1.24) times], a notably longer cumulative duration of off-bed activities [(1.36 ± 0.20) hours], and a significantly shorter duration of first anal exhaust [(13.50 ± 1.27) hours] compared with those in the control group [(1.06 ± 0.12) times, (0.36 ± 0.09) hours, (20.10 ± 2.16) hours, t = -10.84, -39.49, 22.81, all P < 0.001]. Furthermore, the observation group demonstrated a shorter postoperative hospital stay [(8.90 ± 1.34) days], lower hospitalization costs [(55.8 ± 2.1) thousand yuan], and higher patient satisfaction scores [(88.98 ± 5.64) points] compared with the control group [(11.15 ± 1.29) days, (59.4 ± 6.2) thousand yuan, (72.16 ± 3.26) points, t = 10.48, 4.76, -22.36, all P < 0.001]. Conclusion:The implementation of preemptive analgesia intervention among older adult patients undergoing laparoscopic hepatectomy effectively enhances analgesic outcomes, accelerates postoperative recovery, reduces hospitalization duration, and markedly decreases hospitalization costs.

Objective To explore the current status and influencing factors of subjective well-being (SWB) in student nurses so as to provide a basis for formulating corresponding countermeasures.Methods A total of 652 student nurses at a classⅢ grade A hospital in Wenzhou City were selected as subjects by chester sampling from January 2015 to January 2017. They were investigated with the general condition questionnaire and the Chinese version of the Warwick-Edinburgh mental well-being scale (WEMWBS) . Results The total score of WEMWBS was (51.29±9.34) with a middle level in student nurses. Belief, self-report character, sexes, annual household income, body mass index had influences on SWB of student nurses (P<0.01).Conclusions The level of SWB of student nurses needs to be improved. Nurse educators and managers should pay high attention to mental health of student nurses and take positive measures to improve the SWB of student nurses so as to stabilize the construction of nursing team.

Objective To explore the influence of double tutor teaching model on nursing students'ideological quality and comprehensive ability cultivation in nursing clinical teaching. Methods From July 14th,2015 to April 24th,2016,a total of 136 full-time undergraduate nursing students in the Second Affiliated Hospital of Wenzhou Medical University were randomly divided into experimental group (n=68) and control group (n=68) according to random number table method. Post tutor teaching mode was applied as clinical teaching method in the control group,while double tutor teaching mode was administered in the experimental group. Theory,nursing intervention had been tested in each ward during internship for both two groups as well as a unified practice examination form for comprehensive evaluation. Results Theoretical score,operation score,comprehensive evaluation,general scores of the experimental group students were higher than those of the control group (t=27.52,14.82,13.89,14.95;P<0.01). Conclusions Application of double tutorial system teaching mode in clinical nursing practice is conductive to improve theoretical knowledge,operation skills,strengthen the ideological quality of nursing students as well as to elevate their ability of observation,learning,adaptation and communication.

Objective:To explore the effect of nursing intervention based on forgetting curve theory on disease knowledge level and self-management ability of patients with acute pancreatitis.Methods:Using the convenient sampling method, a total of 152 patients with acute pancreatitis who were admitted to the Second Affiliated Hospital of Wenzhou Medical University were selected as the research objects from January 2018 to June 2020. According to the random number table, the research objects were divided into the control group and the observation group, with 76 cases in each group. Patients in the control group were treated with routine nursing interventions, while patients in the observation group were given nursing interventions based on the forgetting curve theory. The intervention time was 1, 2, 4, 7 and 15 days after hospitalization. The self-made Acute Pancreatitis Knowledge Questionnaire and Self-management Questionnaire were used to evaluate the disease knowledge and self-management ability of patients in the two groups before the intervention, 7 days after the intervention, 1 and 3 month after the intervention.Results:There was no statistically significant difference in the acute pancreatitis knowledge scores between the two groups before intervention ( t=0.368, P=0.714) . The acute pancreatitis knowledge scores of patients in the observation group after 7 days of intervention and 1 and 3 months after intervention were higher than those of the control group at the same time, and the differences were statistically significant ( t=5.067, 4.718, 6.361; P<0.01) . There was no statistically significant difference in scores of self-management level of acute pancreatitis of patients between the two groups before intervention ( t=0.160, P=0.873) . The self-management scores of patients in the observation group for acute pancreatitis after 7 days of intervention and 1 and 3 months after intervention were higher than those of the control group at the same time, and the differences were statistically significant ( t=6.379, 7.757, 7.735; P<0.01) . Conclusions:The application of nursing intervention based on forgetting curve theory for patients with acute pancreatitis can improve the disease knowledge level of patients and enhance the self-management ability of them, which is of great significance for patients to maintain their long-term memory of knowledge related to acute pancreatitis.