OBJECTIVE:To investigate the stability of reduced glutathione sodium after mixing with glucose injection (5% or 10%),glucose and sodium chloride injection or 0.9% sodium chloride injection. METHODS:After mixing of reduced glutathione sodium with glucose injection (5% and 10%),glucose and sodium chloride injection,and 0.9% sodium chloride injection,respectively,the 4 mixtures at 0,0.5,1.0,1.5,2.5,3 h after mixing which were stored respectively in 25℃ or 37℃ thermostatic bath were observed in respect of the changes in appearance,pH values,absorbability,contents and the insoluble particles. RESULTS:After mixing of reduced glutathione sodium with glucose injection (5% and 10%),glucose and sodium chloride injection,and 0.9% sodium chloride injection,respectively,the appearance and pH values of the 4 mixtures showed no obvious change,but the contents and the insoluble particles of the 4 mixtures experienced changes of different degree. CONCLUSION:Reduced glutathione sodium is stable in glucose injection (5% or 10%),glucose and sodium chloride injection or 0.9% sodium chloride injection within 2h after mixing.

OBJECTIVE:To establish a method for the contents determination of bacterial endotoxin in Puerarin and Glucose injection.METHODS:The kinetic-turbidimetric technique was adopted to dilute the puerarin glucose injection by2-fold,in which,the contents of bacterial endotoxin was determinated by preliminary interference test and interference test.RESULTS:Diluting the sample by2-fold could effectively eliminate its interference to tachypleus amebocyte lysate(TAL).The average recovery rate was at the range of50%～200%.CONCLUSLON:Compared with the pyrogen rabbit de-termination method and the half-quantity gel method,the kinetic-turbidimetic technique has many merits such as high de-termination sensitivity,short cycle,accurate result and better reproduction quality,etc.

Objective To study the effect of azone,oleic acid,and their combination on the trans-dermal obsorption of baicalin.Methods Improved Franz-cells and HPLC were used for the transdermal study.The effect of azone,oleic acid,and their combination on the transdermal absorption of baicalin was studied using uniform design.Results The 24 h penetration amount of baicalin was obtained as 466.11 ?g/cm~2 and 77.34 ?g/cm~2 when the azone concentration was 10% or the oleic acid concentration was 14%,respectively.When using a combination of azone and oleic acid in the ratio of 10%∶0.5%,the 24 h penetration amount of baicalin was 641.88 ?g/cm~2 and the enhancement ratio of penetration was 146.69.Conclusion Both azone and oleic acid could improve the skin penetration amount of baicalin.Combinated application of azone and oleic acid could further enhance baicalin transdermal delivery rate.The maximum penetration amount of baicalin is obtained by using a combination of azone and oleic acid in the ratio of 10%∶0.5%.

Objective To explore the combination of Astragalus injection combined with Danshen injection in the treatment of nephrotic syndrome. Methods 126 cases of primary nephrotic syndrome were recruited into a control group (66 cases) and a treatment group (60 cases). Both groups were given high-quality protein diet. On this basis, the treatment group was treated with Huangqi injection combined with compound Danshen injection, while the control group was treated with prednisone and furosemide. Both groups received a course of 28 days' treatment. Results The total effective rate was 92% and 56% in the treatment group and the control group respectively, with the difference showing statistical significance (t =4.56,P<0.05) .Conclusion Huangqi injection combined with compound Danshen injection is effective in treatment of nephrotic syndrome.

OBJECTIVE:To compare and analyze blood concentration changes of fluorouracil administered to guinea pigs by different paths.METHODS:The peripheral vein and portal vein blood samples of guinea pigs were taken at different time after administered with fluorouracil in3different ways:group A(by arterial infusion),group B(by intravenous infusion)and group C(by celiac infusion).The blood concentration changes were determined and analyzed by HPLC.RESULTS:The portal vein and peripheral vein blood concentrations in group A and B reached peak value5min after administration with flu?orouracil while group C reached peak at10min;the portal vein blood concentrations in group A reached the highest in the first20min while group C showed the highest value in the last30min.CONCLUSION:Arterial infusion combining celiac infusion path was recommended for local chemotherapy;arterial infusion combining intravenous infusion or intravenous infusion com?bining celiac infusion was recommended for the local therapy while giving consideration to systemic chemotherapy.

OBJECTIVE: To study the bacterial endotoxin test for detecting pyrogen in fructose sodium diphosphate injection . METHODS: The interference of fructose sodium diphosphate injection to the process of bacterial endotoxin test was studied .RESULTS: Fructose sodium diphosphate injection in 10- fold dilution had no interference to the bacterial endotoxin lest .CONCLUSION:Bacterial endotoxin test could be used for pyrogen test of fructose sodium diphosphate injection.

The paper reports one case patient of transcortical sensory aphasia with prominent semantic comprehension disorder. Clinical features were fluent aphasic output, serious auditory comprehension disorder,anomia and prominent disorder in word-lexical level comprehension. Repetition was a little difficult and was remarkably better than other test. There was serious alexia with agraphia, however, copy, series writing, visual spatial function and calculation were intact. His audition was norma1. He could distinguish not only non-word and non-verbal sound but also family member's and acquaintance's sound, but he couldn't understand their talking content. It should be distinguished from pure word-deafness and auditory agnosia- Moreover, we tried to discuss its mechanism.

Objective To analyze the relationship between periodontal diseases and coronary heart disease (CHD),Methods One hundred and sixty-two patients were enrolled into case group and another 162patients were enrolled into control group.Subjects were underwent questionaire investigation and clinic examination.Statistic analysis was performed using multi-factors Logistic regression analysis.Results periodontal diseases ( 95 ％ CI 1.651 - 4.082,OR =1.164 ),working stress ( 95 ％ CI 1.920 - 5.069,OR =3.119),obesity ( 95％ CI 2.298 - 5.133,OR =3.434 ),family history of cardiovascular diseases ( 95％ CI 1.616 - 5.410,OR =2.957),hypertension(95％ CI 2.061 - 6.455,OR =3.647 ) and fat-enriched diet(95％ CI 1.074 - 2.826,OR =1.659 ) were independent risk factors for CHD ( P ＜ 0.05 or P ＜ 0.001 ).Conclusion Dentist.can helo to prevent coronary heart diseases by preventing and curing the periodontal diseases.

OBJECTIVE:To study the effects of common percutaneous enhancers on transdermal permeability of cetirizine hy-drochloride(CET). METHODS:Using pH 7.4 phosphate buffer as receiving solution,accumulative permeation rate as index,mod-ified Franz diffusion cell was used to investigate the effects of common percutaneous enhancers on transdermal permeability of CET in skin of abdomen isolated from mice,such as azone (0.06%-2.5%),oleic acid (0.5%-10%),1,3-propanediol (0.5%-10%),propanetriol(0.5%-5%). RESULTS:1,3- propanediol had no improvement effect on the permeability of CET. 24 h accu-mulative permeation amount of CET in 0.06% azone,0.5% oleic acid and 1% propanetriol were the highest among same types, being 227.94,85.25 and 215.15 μg/cm2,respectively. CONCLUSIONS:Of four percutaneous enhancers,the transdermal enhanc-ing efficiencies from high to low are azone>glycerol>oleic acid>1,3-propanediol. Azone and glycerol have the conspicuous pene-tration enhancing effects.

Objective To study the infection status of hepatitis C virus (HCV) in blood donors .Methods anti‐HCV was detec‐ted by using enzyme linked immunosorbent assay (ELISA) .HCV RNA was detected by using fluorescence quantitative polymerase chain reaction .Results The positive rate of anti‐HCV in blood donors was 0 .07% (109/163 782) .Among 109 positive donors ,80 donors were anti‐HCV positive while only one reagent was used ,and 29 donors were anti‐HCV positive when two reagents were used .80 cases were anti‐HCV positive in first donors and 29 cases were anti‐HCV positive in repeated donors .Among the 80 donors who were anti‐HCV positive while only one reagent was used ,72 samples according with the demand of nucleic acid test were tested by the nucleic acid test ,of whom HCV RNA was negative .Conclusion The positive rate of anti‐HCV in Yancheng donors could be lower than general population .There might be no change of positive rate of anti‐HCV in blood donors during the three years .The positive individuals could be negative in nucleic acid test while only one reagent was used in ELISA test .