OBJECTIVE:To investigate the stability of reduced glutathione sodium after mixing with glucose injection (5% or 10%),glucose and sodium chloride injection or 0.9% sodium chloride injection. METHODS:After mixing of reduced glutathione sodium with glucose injection (5% and 10%),glucose and sodium chloride injection,and 0.9% sodium chloride injection,respectively,the 4 mixtures at 0,0.5,1.0,1.5,2.5,3 h after mixing which were stored respectively in 25℃ or 37℃ thermostatic bath were observed in respect of the changes in appearance,pH values,absorbability,contents and the insoluble particles. RESULTS:After mixing of reduced glutathione sodium with glucose injection (5% and 10%),glucose and sodium chloride injection,and 0.9% sodium chloride injection,respectively,the appearance and pH values of the 4 mixtures showed no obvious change,but the contents and the insoluble particles of the 4 mixtures experienced changes of different degree. CONCLUSION:Reduced glutathione sodium is stable in glucose injection (5% or 10%),glucose and sodium chloride injection or 0.9% sodium chloride injection within 2h after mixing.

OBJECTIVE:To establish a method for the contents determination of bacterial endotoxin in Puerarin and Glucose injection.METHODS:The kinetic-turbidimetric technique was adopted to dilute the puerarin glucose injection by2-fold,in which,the contents of bacterial endotoxin was determinated by preliminary interference test and interference test.RESULTS:Diluting the sample by2-fold could effectively eliminate its interference to tachypleus amebocyte lysate(TAL).The average recovery rate was at the range of50%～200%.CONCLUSLON:Compared with the pyrogen rabbit de-termination method and the half-quantity gel method,the kinetic-turbidimetic technique has many merits such as high de-termination sensitivity,short cycle,accurate result and better reproduction quality,etc.

Objective To study the effect of azone,oleic acid,and their combination on the trans-dermal obsorption of baicalin.Methods Improved Franz-cells and HPLC were used for the transdermal study.The effect of azone,oleic acid,and their combination on the transdermal absorption of baicalin was studied using uniform design.Results The 24 h penetration amount of baicalin was obtained as 466.11 ?g/cm~2 and 77.34 ?g/cm~2 when the azone concentration was 10% or the oleic acid concentration was 14%,respectively.When using a combination of azone and oleic acid in the ratio of 10%∶0.5%,the 24 h penetration amount of baicalin was 641.88 ?g/cm~2 and the enhancement ratio of penetration was 146.69.Conclusion Both azone and oleic acid could improve the skin penetration amount of baicalin.Combinated application of azone and oleic acid could further enhance baicalin transdermal delivery rate.The maximum penetration amount of baicalin is obtained by using a combination of azone and oleic acid in the ratio of 10%∶0.5%.

The paper reports one case patient of transcortical sensory aphasia with prominent semantic comprehension disorder. Clinical features were fluent aphasic output, serious auditory comprehension disorder,anomia and prominent disorder in word-lexical level comprehension. Repetition was a little difficult and was remarkably better than other test. There was serious alexia with agraphia, however, copy, series writing, visual spatial function and calculation were intact. His audition was norma1. He could distinguish not only non-word and non-verbal sound but also family member's and acquaintance's sound, but he couldn't understand their talking content. It should be distinguished from pure word-deafness and auditory agnosia- Moreover, we tried to discuss its mechanism.

OBJECTIVE: To study the bacterial endotoxin test for detecting pyrogen in fructose sodium diphosphate injection . METHODS: The interference of fructose sodium diphosphate injection to the process of bacterial endotoxin test was studied .RESULTS: Fructose sodium diphosphate injection in 10- fold dilution had no interference to the bacterial endotoxin lest .CONCLUSION:Bacterial endotoxin test could be used for pyrogen test of fructose sodium diphosphate injection.

OBJECTIVE:To compare and analyze blood concentration changes of fluorouracil administered to guinea pigs by different paths.METHODS:The peripheral vein and portal vein blood samples of guinea pigs were taken at different time after administered with fluorouracil in3different ways:group A(by arterial infusion),group B(by intravenous infusion)and group C(by celiac infusion).The blood concentration changes were determined and analyzed by HPLC.RESULTS:The portal vein and peripheral vein blood concentrations in group A and B reached peak value5min after administration with flu?orouracil while group C reached peak at10min;the portal vein blood concentrations in group A reached the highest in the first20min while group C showed the highest value in the last30min.CONCLUSION:Arterial infusion combining celiac infusion path was recommended for local chemotherapy;arterial infusion combining intravenous infusion or intravenous infusion com?bining celiac infusion was recommended for the local therapy while giving consideration to systemic chemotherapy.

Objective To explore the combination of Astragalus injection combined with Danshen injection in the treatment of nephrotic syndrome. Methods 126 cases of primary nephrotic syndrome were recruited into a control group (66 cases) and a treatment group (60 cases). Both groups were given high-quality protein diet. On this basis, the treatment group was treated with Huangqi injection combined with compound Danshen injection, while the control group was treated with prednisone and furosemide. Both groups received a course of 28 days' treatment. Results The total effective rate was 92% and 56% in the treatment group and the control group respectively, with the difference showing statistical significance (t =4.56,P<0.05) .Conclusion Huangqi injection combined with compound Danshen injection is effective in treatment of nephrotic syndrome.

OBJECTIVE: To investigate the effect of arsenic trioxide (As2O3) combined with cisplatin on expression of RASSF1A in nude mice with human nasopharyngeal carcinoma xenograft. METHOD: The models of human poorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into four groups, control group (NaCl group), As2O3 group, DDP group and As2O3 + DDP group. The expression of RASSF1A mRNA and protein were detected by Real-time RT-PCR and immunohistochemistry respectively. The methylation rate of RASSF1A promoter CpG islands was analyzed by HRM. RESULTS: Experimental groups could obviously inhibit the growth of tumor and up-regulate the expression of RASSF1A. The methylation rate of RASSF1A in transplanted tumors in experimental groups was lower than the control group. Especially As2O3 combined with DDP were superior to the single drug use. CONCLUSION As2O3 inhibits the growth of human nasopharyngeal carcinoma cell strain CNE2 xenograft in nude mice and increases mRNA expression of RASSF1A. As2O3 inhibits the malignant phenotypes of human nasopharyngeal carcinoma cells and reverses hypermethylation of RASSF1A.
Animals ; Arsenic Trioxide ; Arsenicals ; pharmacology ; Carcinoma ; Cell Line, Tumor ; Cisplatin ; pharmacology ; DNA Methylation ; drug effects ; Humans ; Mice ; Mice, Nude ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; genetics ; pathology ; Neoplasm Transplantation ; Oxides ; pharmacology ; Tumor Suppressor Proteins ; genetics ; Xenograft Model Antitumor Assays

Objective To investigate the effects of angiotensin Ⅱ (Ang Ⅱ ) and losartan on the expression of small ubiquitin-related modifier(SUMO) protein (SUMO1 ,SUMO2/3) in cultured rat glomerular mesangial cells(GMCs) .Methods In vitro cul-tured HBZY-1 rat GMCs were divided into 5 groups:normal control group(NC group) ,different concentrations of Ang Ⅱinterven-tion groups(A1 ,A2 ,A3 groups) and losartan treatment group(MT group) .The expression of SUMO1 and SUMO2/3 protein and mRNA of each group was measured by Western blot and RT-PCR .Results Compared with the NC group ,the expression of SU-MO1 and SUMO2/3 protein and mRNA in the Ang Ⅱintervention groups and the losartan treatment group was increased signifi-cantly (P<0 .01);Compared with the Ang Ⅱintervention groups ,the expression of SUMO1 and SUMO2/3 protein and mRNA in the losartan treatment group had no statistically significant difference .Conclusion Ang Ⅱ up-regulates the expression of SUMO protein in rGMCs by a dose-dependent manner in certain range ,this effect is not blocked by losartan ,Ang Ⅱ may be involved in the pathogenesis of diabetic nephropathy .

Objective To compare the quality of emergence from TCI of sufentanil and remifentanil supplementing propofol-sevoflurane anesthesia in patients undergoing radical colo-rectal cancer resection.Methods Forty ASA Ⅰ or Ⅱ patients of both sexes aged 40-64 yr undergoing elective radical colo-rectal cancer resection were allocated into 2 groups ( n =20 each):sufentanil group (group S) and remifentanil group (group R).Anesthesia was induced with propofol TCI at plasma concentration (Cp) of 4.0 μg/ml in both groups and sufentanil TCI (effect-site concentration Ce =0.4 ng/ml ) or remifentanil TCI ( Cp =4.0 ng/ml).Tracheal intubation was facilitated with vecuronium 0.1 mg/kg.The patients were mechanically ventilated (VT =8-10 ml/kg,RR =12-16 bpm).PErCO2 was maintained at 30-40 mm Hg.Anesthesia was maintained with propofol TCI-sevoflurane supplemented with sufentanil (Ce=0.25 ng/ml) or remifentanil (Cp=2.5 ng/ml).The depth of anesthesia was maintained at Narcotrend index of 37-56 by adjusting Cp of propofol TCI and sevoflurane concentration.The infusion of sufentanil was discontinued at 40 min before the conclusion of the operation while remifentanil was administered until the end of surgery.The incidence of postoperative adverse events,the time from the end of operation to eye openg and the time to extubation were recorded.Reesults The two groups were comparable with respect to demographic data.Neither group developed prolonged emergence and respiratory depression but the time from the end of operation to eye opening and the time to extubation were significantly longer in group S than in group R.The incidence of hypertension and tachycardia,agitation,shivering aad coughing were significantly lower in group S than in group R.Conclusion The quality of emergence from sufentanil supplementing propofol-sevoflurane anesthesia is higher than that from remifentanil.