Objective To explore the cognitive changes of patients with MCI using the Montreal Cognitive Assessment(MOCA) for elderly preliminarily.Methods 85 normal controls (NC),117 subjects with mild cognitive impairment (MCI) and 73 patients with Alzheimer's disease (AD) were assessed with the MOCA.Results There were significant differences among the three groups in all items of MOCA(F=258.66,P＜0.01).Significant differences were observed in almost all the sub-tests between MCI group and NC group or MCI group and AD group(visuopatial F=54.86,P＜0.01;naming F=17.30,P＜0.01;attention F=82.50,P＜0.01;language F=25,88,P＜0.01;abstraction F=15.00 ,P＜0.01;delayed recall F=130.49,P＜0.01;orientation F=176.09,P＜0.01.).The most significant differences were found in delayed recall and orientation among three groups(F=176.09,P＜0.01;F=130.49,P＜0.01.).At 26 cut-point,The results of screening of the MOCA agree with the gold standard of clinical diagnose for the patient with MCI.The agreement rate for observation was 0.93.The agreement rate by chance was 0.61.The Kappa was 0.85.Conclusions The MOCA appropriately define MCI,NC and AD in their cognitive function,it has good discriminant validity.The test of delayed recall and orientation may be more sensitive in the detection of the older people.The MOCA is a useful screening instrument for the patient with MCI.

Objective To compare the therapeutic effect in two kinds of inhalation in infant asthmatic bronchitis.Methods Two hundred and twelve asthmatic bronchitis children were randomly divided into group A (103 cases) and group B (109 cases).The children in two groups were treated with conventional therapy,including antibiotics,fluids and other treatment.The children in group A were given budesonide 1 mg,terbutaline 5 mg plus 0.9％ sodium chlorine in 2 ml,with oxygen driven inhalation therapy,2-4 times/d.The children in group B were given albuterol sulfate inhalation aerosol 1-4 spray/times,4-6 times/d,inhaled fluticasone quantitative aerosol spray 1-2 spray/times,2 times/d,15 s/times.The course of two groups was 5-7 d.The therapeutic effect was compared.Results The total effective rate in group A and group B were 92.2 ％ (95/103) and 97.2 ％ (106/109),there was no significant difference (x2 =2.73,P ＞ 0.05).The relieve time of cough,gasping and wheezing,hospital stay in group B were shorter than those in group A [(12.00 ± 2.75) h vs.(29.00 ± 1.25) h,(10.00 ± 1.45) h vs.(31.00 ± 2.75) h,(13.00 ± 1.55) h vs.(33.00 ± 2.33) h,(7.20 ± 0.65) d vs.(8.70 ± 1.35) d],the cost of hospitalization in group B was lower than that in group A [(2.00 ± 0.30) thousand Yuan vs.(2.50 ± 0.55) thousand Yuan],there were significant differences(P ＜ 0.05).Conclusions The albuterol inhalation solution combined with fluticasone has the advantages of rapid efficacy,short course,low cost,easy to accept and adjust.It is worth to spread in primary hospital.

Objective To explore the efficacy and safety of different doses of budesonide mixed suspension atomization inhalation in children with post infectious cough,and provide reference for the rational use of budesonide.Methods One hundred and twenty children with post infectious cough aged 2-14 years old were selected and divided into three groups by random digits table method (40 cases in each group).Control group was treated with 4 mg once montelukast sodium chewable tablets for 2-5 years old children,or 5 mg once for 6-14 years old children.Low-dose group was treated 2 times/d with a dose of 0.5 mg budesonide mixed suspension diluted in normal saline to 4 ml based on control group and high-dose group was treated 2 times/d with a dose of 1.0 mg budesonide mixed suspension diluted in normal saline to 4 ml based on control group.The outcome of curative effects were recorded and analyzed after 7 days of treatment.Results The total efficacy rate was 70.0％(28/40),52.5％(21/40),35.0％(14/40) in high-dose group,low-dose group and control group after 3 days of treatment,and there was significant difference between high-dose group and control group (P＜ 0.01).The total efficacy rate was 92.5％(37/40),90.0％(36/40),72.5％ (29/40) in high-dose group,low-dose group and control group after 7 days of treatment,and there was no significant difference(P ＞ 0.025).Few adverse events associated with inhaled corticosteroids,such as thrush,sore throat,hoarseness were observed in three groups.Conclusions Budesonide mixed suspension atomization inhalation alleviates symptom of post infectious cough significantly with no obvious side effects,even in high-dose group which has a more outstanding efficacy.Therefore,this therapy is valuable in clinical application.

Objective To study the efficiency of reversing MDR by suppressing MDR1 gene with RNAi in K562/ADM cells. Methods For reversing MDR by RNAi technology, two different short hairpin RNAs (shRNAs) were designed and constructed into pGenSil-1 plasmid, respectively. They were then transfected into the highly adriamycin-resistant K562/ADM cell line. The RNAi effect on MDR was evaluated by real-time PCR, and Rhodamine123 (Rh123) efflux assy. Results The stable transfected clones showed varied degrees of reversal of MDR phenotype. Surprisingly, the MDR phenotype was completely reversed in two transfected clones. Conclusion This study demonstrates that MDR can be reversed by the shRNA-mediated RNAi in K562/ADM cells, which provides a valuable clue as to sensitizing multidrug-resistant hepatoma cells to anti-cancer drugs.

OBJECTIVE: To detect whether there is mixing of norfloxacin in loperamide hydrochloride capsules by UPLC-MS. METHODS: The separation of norfloxacin was performed on C18 column with column temperature set at 35 ℃, and the mobile phase was composed of acetronitrile and 0.1% formic acid (gradient elution) with a flow rate of 0.3 mL?min-1. The sample size was 10 ?L. ESI source was applied with capillary tube tension at 3.0 kV and ion source temperature at 110 ℃, and scan mode was multiple reaction monitor. RESULTS: It has been proved that there was norfloxacin in 2 of the total 5 batches of samples based on the data of retention time of RP-HPLC including ultraviolet spectra, the quasi-molecular ion and the second order mass spectra split fragments. CONCLUSION: The method is highly selective and sensitive, and can be used to detect whether norfloxacin has been illegally added into loperamide hydrochloride capsules.

Objective To study the ultrastructure changes of hippocampus pyramidal cells and capillaries in genetically diabetic mice C57BL/KsJ (db/db).Methods We chose 5 obese C57BL/KsJ db/db mice of 6 weeks old with fast blood glucose (FBG) higher than 11 1mmol/L as diabetic group and 5 normal weight C57BL/KsJ (?/+) mice with FBG lower than 6 0mmol/L as normal group. Mice were killed at 30 weeks and hippocampus samples were embedded in Epon 812. Thin sections were cut with ultrathin microtome and observed with electron microscopes. Results Pyramidal cells of hippocampus in diabetic mice had significant retrograde changes. The basement membrane of capillaries thickened significantly and endothelial cells and pericytes degenerated.Conclusion The significant pathological changes in hippocampus pyramidal cells and capillaries of diabetic mice may related to dysfunction of cognition.

Objective: To explore the effect of comprehensive intervention to correct parental unhealthy rearing behavior on middle-school students. Methods: Eight freshman classes were randomly extracted from two key junior middle schools, four classes as the experimental groups, the other four as the control group. EMBU was applied to measure the difference before and after intervention of both groups. The comprehensive intervention was used, mainly the individual family mental health education and group family education. Results: After intervention, there were significant improvement in the parents' emotional warmth and understanding factors of experimental group(53.0?10.6/49.3?10.1, 55.7?10.9/50.4?10.5,t= 4.24 or 5.80,P

0.05). ?-EP level of psoriatic patients 1 and normal controls 1 was significantly higher than that of psoriatic patients 2 and normal controls 2 respectively (P0.05) . Conclusion It suggests that there is the correlation between serum ?-EP level and psychological factors. ?-EP is likely to participate in adjusting the whole body state, which provides the foundation for psychologic treatment.

Objective To identify the effect of using modified 99 type China hemp service shoes and socks on preventing servicemen from skin superficial fungal infection during the drill.Methods A double-blinded control study was carried on in 2 months test with 16 testees who wore the modified 99 type China hemp service shoes and socks.Every testee was dispensed 1 pair of shoes and 3 pairs of socks.The left socks were produced in different material and way from the right one in each pair.Clinical examination of feet skin primary lesions and fungal test on each individual testee were done at the beginning of this research,as well as in the middle and the end.Results The clinical symptoms of 16 testees' feet skin were obviously relived during the 2 months test.4 of 16 testees claimed that there were more obviously relieving of feet symptoms on left than right.8 of 16 testees which were positive of fungal test at the beginning of the research claimed that their feet symptoms were lessened,and 2 of the above 8 were turned to negative of fungal test after 8 weeks,however the other 6 were positive yet.And the fungi culture was done on these 6 above testees who were still positive on fungal test.2 cases were Trichophyyon gypseum,while other 4 cases were Trichophyyon rubrum.Conclusion The modified 99 type China hemp service shoes and socks can relieve the clinical symptoms and prevent fungal infection that occurs during the drill.

Pharmacognostic studies on the sollrce, shape, morphology, microscopic structrue and physico-chemical properties of the genitalia of mink were carried out in comparison with those of deer,donkey and dog with similar pharmacologic actions. Results showed that the genitalia of mink has its specific features in shape,mi croscopic structure and physico-chemical properties which can be used as the main characteristics to distin guish it from those of other animals. Chemical analysis showed that the genitalia of mink was rich in aminoacids and its zinc,manganese and calcium contents exceeded those present in the genitalia of other ani mals