Herpes simplex virus type 1 (HSV-1), as a pathogen usually leading to herpes labialis, has been found to cause more cases of genital herpes and be associated with the occurrence of neurological diseases such as Alzheimer′s disease (AD). AD is a chronic and progressive degenerative disease of the central nervous system, the pathogenesis of which has not been fully elucidated, and currently there is a lack of drugs that can effectively treat the disease. The hypothesis that HSV-1 infection is involved in the pathogenesis of AD has been discussed for a long time, but there is no definite evidence to prove it. This article briefly reviewed the research progress in the pathogenesis of AD caused by HSV-1.

Objective To search for the effect of benidipine,a kind of Ca2+ blocker,on NO and ET-1 of patients with coronary heart disease.Methods Benidipine(2~4 mg/d)were given to the patients with coronary heart disease diagnosed definitely for 8 weeks.Forty healthy candidates were enrolled in the control group,and their morning fasting blood were tested for NO and ET-1 levels at the 4th,8th week in order to compare with the control group.Results Blood NO level of CHD patients was lower and blood ET-1 level was higher than the control group remarkably.After treatment NO level was increased and ET-1 level was decreased separately.Conclusion Benidipine can increase NO level and decrease ET-1 level,which demonstrated the protective effect of benidipine for endothelia.

OBJECTIVE:To establish a method for the content determination of L-shikonin in Zilian liniment and evaluate the uncertainty by 2 calculation methods. METHODS:HPLC was performed on the column of Waters C18 with mobile phase of metha-nol-0.025 mol/L phosphate(85:15,V/V)at flow rate of 1.0 ml/min,the detection wavelength was 516 nm,column temperature was room temperature,and the injection volume was 10 μl. Quantitative analysis was conducted for the process and procedures calculat-ed by standard curve method and external standard method,and the uncertainty was evaluated. RESULTS:The linear range of L-shi-konin was 4.024-80.48μg/ml;RSDs of precision,stability and reproducibility test were lower than 2.0%;recovery was 88.5%-93.1%(RSD=2.0%,n=6). Standard curve method was used to determine the content,and the expanded uncertainty was 0.70 μg/ml;ex-ternal standard method was used to determine the content,and the expanded uncertainty was 0.30 μg/ml. CONCLUSIONS:Stan-dard curve method is more economical,more convenient and lower uncertainty in the content determination of L-shikonin in Zilian liniment.

The diabetic patients died of cardiovascular disease mainly. However, the molecular and cellular mechanisms of diabetes complicated with atherosclerosis were still unclear. Endoplasmic reticulum (ER) stress may play a vital intermediate role in the progression of diabetic atherosclerosis. Both of diabetic hyperglycemia induced hexosamine pathway and diabetes induced macrophage insulin resistance can lead to ER stress. The unfolded protein response (UPR) produced by ER stress can be observed throughout the whole development course of atherosclerosis. In addition, ER stress also induced lipid deposition, cell apoptosis and inflammatory response, all of which was important for the progression of atherosclerosis. Though ER stress may be key for diabetes induced atherosclerosis, which pathway played the central role and the interaction between cytokines still need to be investigated. So that we could provide individual therapeutic scheme for treatment as well as prevention of diabetic complication.

Objective To investigate the changes and clinical significance of platelets aggregation rate of patients with coronary heart disease before and after percutaneous transluminal coronary stenting Methods There are 13 patients underwent coronary angiography and 20 patients underwent percutaneous transluminal coronary stenting Their blood samples were taken from the coronary sinus and coronary artery before and after interventional therapy Results The level of platelets aggregation rate in percutaneous transluminal coronary stenting group was higher after interventional therapy ( P

ObjectiveTo investigate the adjuvant effect of heatshock protein 110(HSP110) on the immune responses induced by an altered peptide ligand of human papilloma virus type 16 E711-20 peptide (HPV16E711-20).Methods The complex of HSP110 and an altered peptide ligand of HPV16E711-20 was constructed in vitro.Fifteen 6-week-old C57BL/6 female mice were randonly and equally divided into 3 groups,including complex group,ligand group,and phosphate buffered solution (PBS) group,to receive intraperitoneal immunization with the complex (100 μg),peptide (10 μg),and PBS (100 μl) respectively.Immunization was carried out with an interval of 2 weeks for 2 times.Two weeks after the last immunization,the mice were sacrificed followed by the isolation of splenocytes.MTT assay was performed to evaluate the proliferation activity of splenocytes,intracellular staining for interferon(INF)-γ to detect cytotoxic T lymphocytes (CTLs),standard chromium-51 (51Cr) release assay to estimate the lethal effect of specific CTLs on target cells.Statistical analysis was performed by using t test with SPSS 10.0 software.Differences were considered as statistically significant when the P value was less than 0.05.ResultsA significant increase was observed in the proliferation index ( 1.87 ± 0.122 vs.0.32 ± 0.071,t =4.01,P ＜ 0.01 ) of,and percentage of CD8+IFN-γ+ T lymphocytes(3.9％ vs.0.4％,t =3.88,P ＜ 0.01 ) among splenocytes from the complex group compared with the ligand group.At the effector-to-target ratio of 100 ∶ 1,50 ∶ 1,25∶ 1 and 12.5 ∶ 1,the death rate of target cells was 54.7％,72.2％,61.5％ and 39.8％ respectively after incubation with CTLs from the compleximmunized mice,higher than that from the ligand-immunized mice (35.2％,49.3％,28.1％,17.4％,respectively).ConclusionHSP110 could enhance the immunological effect of the altered peptide ligand of HPV16E711-20,and can serve as an immunological adjuvant.

Objective To evaluate the effectiveness of control and prevention of iodine deficiency disorders(IDD) in Xinjiang Production and Construction Corps. Methods According to the national requirement and the situation of Xinjiang Production and Construction Corps, thirty sampling units were selected by the“probability proportionate to size” method; one school was chosen in each sampling unit; forty children (half male half female) aged 8 - 10 years old were chosen in each school. Family salt samples were collected to detect salt iodine content, and the goiter was determined. Twelve children(half male half female) from the 40 children were selected; urine samples were collected to detect urinary iodine content. Twenty fifth-grade children in each school and five housewives around each school were selected, and the survey of health education knowledge was conducted. Salt iodine was measured using direct titration (GB/T 13025.7-1999);the volume of thyroid was detected by ultrasound ( WS 276-2007 ); urinary iodine was measured by arsenic Ce catalytic spectrophotometry method (WS/T 107-2006). Results A total of 1 195 household salt samples were tested. The mean of salt iodine was (30.91 ± 6.99)mg/kg;the coverage rate of iodized salt was 97.49%(1 165/ 1 195);the qualified rate of iodized salt was 98.54%(1 148/1 165); and the consumption rate of qualified iodized salt was 96.07%(1 148/1 195). Twelve hundred and twenty children aged 8-10 years old were examined;forty-two goiters were examined;and goiter rate was 3.44%. Four hundred and seven urine samples were tested, the median of urinary iodine was 235.31 μg/L;< 50 μg/L accounted for 1.47%(6/407); and 50 - < 100 μg/L accounted for 6.63%(27/407). The awareness rates of IDD health education knowledge of the fifth-grade students and housewives were 84.39%(1 519/1 800) and 84.44%(380/450), respectively. Conclusion The level of IDD control and prevention in Xinjiang Production and Construction Corps has been up to the national standard of eliminating IDD.

Objective:To evaluate the efficacy and safety of low molecular weight heparin and unfarction heparin in patients with coronary heart disease during percutaneous coronary intervention by investigating the MACE beteewn the percutaneous coronary intervention procedure and post percutaneous coronary intervention 72 hours.Methods:200 patients with coronary heart disease who accepted percutaneous coronary intervention were investigated in this study.According to the anticoagulants,these patients were divided into LMWH subgroup(117 cases) and UFH subgroup(83 cases).According to conventional method,the MACE what happened during percutaneous coronary intervention procedure and post percutaneous coronary intervention 72 hours come from each group of patients was investigated and these statistics were analysised so that evaluate the efficacy and safety of low molecular weight heparin and unfarction heparin.Results:(1) There were no statistical significance in baseline characteristics between the each group (P＞0.05).(2) There were statistical significance in the incidence of TIMI flow slows between the each group (P＜0.05),low molecular weight heparin is superior to unfarction heparin in terms of efficacy.(3)There were no statistical significance in death between the each group (P＞0.05),but there were statistical significance in bleeding / hematoma complications,and other (pericardial tamponade,chest pain,cardiogenic shock,cardiac rapture,ventricular septal perforation,ventricular tachycardia,ventricular fibrillation,cardiac arrest,Aspen attack,stent thrombosis and so on) between the each group (P＜0.05),low molecular weight heparin less adverse reactions,higher safety.Conclusion:Low molecular weight heparin in the percutaneous coronary intervention effect is more significant,and less than UFH adverse reactions and high safety,more suitable for percutaneous coronary intervention anticoagulant therapy.

In this paper,the low rate of commercial of biomedical engineering scientific achievements transfer in military universities was discussed in detail.The main problems were analyzed.The commercial strategies of biomedical engineering scientific achievements transfer inmilitary universities were propound.

Objective: Auricularid auricula polysaccharide was isolated from Auricularia auricula, and then iron(Ⅲ)-Auricularia auricula polysaccharide complex(APC) was prepared. Methods: APC was synthesized with FeCl 3 and its physico-chemical property and bioavailability were determined. Results: APC was able to dissolve in water and its solution assumed neutral, and this complex did not precipitate at pH from 3 to 11 in water solution, so APC was very stable. Fe(Ⅲ) in APC could be reduced to Fe(Ⅱ) by ascorbic acid. Extracorporeal dissolubility showed Fe(Ⅲ) in APC could be exhibited in 6 h. Bioavailabity studies showed the iron from APC could be well absorbed and maintained longer in serum concentration. Conclusion: APC was able to have better bioavilability as an iron supplement.