Objective To investigate the correlation of arterial compliance and carotid atherosclerosis in patients with type-2 diabetes.Methods Eighty cases of type-2 diabetic patients and 80 control subjects were recruited into the study.Brachial-ankle pulse wave velocity(BaPWV)and ankle-brachial index(ABI)were measured with atherosclerosis diagnostic devices VP-1000.The inner-membrane and medium-membrane thickness(IMT)of the common carotid artery and plaque were detected with color Doppler ultrasound.The difference of the pulse wave velocity in the subjects with carotid plaques were compared between the two groups.The correlations of carotid IMT with BaPWV and ABI were also analyzed.Results In the diabetic patients group,BaPWV([1752 ± 213]cm/s)was higher than that of normal control group([1279 ± 159]cm/s)(P ＜ 0.01);ABI in diabetic patients group(0.95 ± 0.13)was lower than that of control group(1.28 ± 0.17),(P ＜ 0.01).In the diabetic patients group,the incidences of carotid IMT thickening(45.0％)and plaques(35.0％)were both higher than that of control group(27.5％ and 10.8％,respectively)(P ＜ 0.05).In the diabetic patients group,the cases with carotid plaques had a higher BaPWV([1810 ± 296]cm/s)than those without([1480 ± 304]cm/s)(P ＜ 0.01).BaPWV was positively correlated with carotid IMT(r =0.271,P ＜ 0.05).ABI and IMT was negatively correlated(r =-0.406,P ＜ 0.05).Conclusion BaPWV and carotid IMT was positively correlated,while ABI and IMT was negatively correlated.BaPWV and ABI are indicators which are practicable and effective to evaluate the stiffness of peripheral arteries in patients with diabetes.

Objective To investigate the effects of dexamethasone (DEX) on vascular endothelial growth factor( VEGF) in cerebrospinal fluid of rabbits with bacterial meningitis. Methods A total of 36 rabbits were assigned to study,which were randomly divided into meningitis model group (MOD) .dexametha-sone-treated group (DEXT) and control group( CON). CSF was sampled for determining at 6 h, 12 h and 24 h after injection of E. coli suspension. The concentration of VEGF in every CSF sample was determined quantitatively by ELISA. Results There were higher concentrations of CSF VEGF at6h,12h and 24hin M0D(( 1219 ±176) ng/L,( 1343 ±160) ng/L,(981 ±134) ng/L) than that in CON( (374 ±172) ng/L, (370 ± 169) ng/L,(367 ± 171) ng/L) (P<0.01). There was higher brain water content in M0D( (80.8 ± 0.5) % ) than that in CON( (80.0 ± 0.5) % ) (P < 0.01). There was positive correlation between the brain water content and the concentration of CSF VEGF at 24 h( r - 0.919,P < 0.01). Compared with MOD, the concentrations of CSF VEGF in DEXT at 6 h, 12 h,24 h ((941 ±147) ng/L, (1083 ± 123) ng/L, (825 ± 66) ng/L) were decreased significantly(P <0.05), the brain water content was less ((80.4 ±0.5) %) (P < 0.05). Conclusion The secretion of VEGF markedly increases in the pathological process of bacterial meningitis. VEGF contributes to the damage of blood brain barrier and the formation of brain edema. DEX can decrease the degree of brain edema by suppressing the generation of VEGF and lightening the damage of blood brain barrier.

Objective To determine the relationship between brachial-ankle pulse wave velocity(baPWV)and Rho associated coiled coil protein kinase(ROCK)activity with the cardiovascular disease(CVD).Methods 168 patients with CVD were divided in-to the CVD group(123 cases)and the non-CVD group(45 cases)based on the results of coronary angiography.The baPWV value and the ROCK activity were detected.The CVD group were subdivided into the single vessel,double vessels and multiple vessels le-sions groups.Results The baPWV value and ROCK activity in the CVD group were significantly higher than those in the non-CVD group(P<0.05).The more the lesion vessels,the higher the baPWV value(P<0.05).Conclusion The baPWV and ROCK activi-ty are associated with CVD.Therefore,the combined measurement of baPWV and ROCK levels could be used to assess CVD and its risk degree.

Objective To explore the effects of dead box 1 (DDX1) gene on cell apoptosis,proliferation and cell cycle of neuroblastoma(NB) cells.Methods SK-N-BE(2)/blank,SK-N-BE(2)/shV and SK-N-BE (2)/shDDX1 cells were seeded in 96-well plates,which grew in good condition and in the logarithmic growth phase,5 000 cells were inoculated in each well and 5 repeated holes were set.Cell count kit 8 (CCK-8) was used to detect the cell number at 12 h,24 h,36 h,48 h,and the average was calculated.The time (hour) was set as abscissa,the optimal density (A) value at 450 nm was set as vertical axis,and the growth curves of these 3 cells were drawn to investigate the effects of DDX1 on the proliferation of NB cells.After 24 h,flow cytometry (PI staining) was used to detect the apoptosis of SK-N-BE (2)/blank,SK-N-BE (2)/shV and SK-N-BE (2)/shDDX1 cell lines to observe the effects of DDX1 on the apoptosis of NB cells.After 24 h,flow cytometry (PI staining) was used to detect the proportion of SK-N-BE(2)/blank,SK-N-BE(2)/shV and SK-N-BE(2)/shDDX1 cells at G1,S,M,G2 stage to observe the effects of DDX1 on the cell cycle of NB cells.Results SK-N-BE (2)/shDDX1 cell proliferation was significantly lower than SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,that was to say,DDX1 knockdown reduced the cell proliferation of NB.According to the flow cytometry results,the total average apoptosis rate was 5.28％ in SK-N-BE (2)/shV cells,and the total average apoptosis rate was 9.99 ％ in SK-N-BE (2)/shDDX1 cells.The number of apoptotic SK-N-BE (2)/shDDX1 cells was significantly higher than the number of SK-N-BE (2)/blank and SK-N-BE (2)/shV cells,which indicated that DDX1 knockdown increased tumor cell apoptosis of NB.Compared with SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,the cell cycle of SK-N-BE(2)/shDDX1 cells was arrested,and the proliferation was affected.Conclusions After DDX1 expression is inhibited,the cell cycle of NB cells are affected,the cell apoptosis is increased,and the cell proliferation is reduced.

Objective To explore the effects of dead box 1 (DDX1) gene on invasion,migration and drug resistance capability of neuroblastoma(NB) cells.Methods According to the virus drop degree,the appropriate amount of target virus(Lenti-DDX1-MIR virus liquid,drop degrees 1012 TU/L) and negative control virus(Lenti-EGFP virus liquid,drop degrees 3 × 1011 TU/L) (multiplicity of infection was 10) were added into 2 hole cells,respectively.SK-N-BE(2)/blank,SK-N-BE(2)/shV and SK-N-BE(2)/shDDX1 cells which grew in good condition were cultured.Transwell chamber was used to detect the invasion,and cell staining was made with crystal violet.The researchers calculate 5 field counting in each small room and calculate the average cell invasion rate.Transwell chamber was used to detect the migration,and cell staining was made with crystal violet.The 570 nm absorbance values was tested with enzyme linked immunosorbent assay (ELISA) reader,and cell migration was calculated.The researchers used 50 mg Cisplatin,solution 10 g/L mother liquor standby with 5 mL dimethyl sulphoxide,and 50 mg Doxorubicin,solution 1 g/L mother liquor standby with PBS.Drugs were added to the cell culture plate,and Doxorubicin final concentration was 1.0 mg/L,and Cisplatin final concentration was 2.5 mg/L,and photographic record was documented after drug treatment for 24 h.Cell Count Kit-8 (CCK-8) was used to detect the drug sensitivity to NB cells to Doxorubicin and Cisplatin.Results Transwell results showed that,cell invasion concentration in SK-N-BE (2)/shDDX1 was 60％ compared with SK-N-BE (2)/blank and SK-N-BE (2) /shV;Crystal violet staining showed that cell invasion of SK-N-BE(2)/shDDX1 was significantly weaker than that of SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,that is to say,DDX1 knockdown reduced the cell invasion of NB.Transwell results showed that,cell migration concentration in SK-N-BE(2)/shDDX1 was 50％ compared with SK-N-BE(2)/blank and SK-N-BE(2)/shY;Crystal violet staining showed that cell migration of SK-N-BE(2)/shDDX1 was significantly weaker than that of SK-N-BE(2)/blank and SK-N-BE(2)/shV cells,that is to say,DDX1 knockdown reduced the cell migration of NB.With DDX1 knockdown,24-h inhibition rate of SK-N-BE (2)/shDDX1 cell was 1.93 times of SK-N-BE (2)/shV cell with 1.0 mg/L Doxorubicin,24 h inhibition rate of SK-N-BE(2)/shDDX1 cell was 1.38 times of SK-N-BE(2)/shV cell with 2.5 mg/L Cisplatin.DDX1 knockdown could increase the Doxorubicin and Cisplatin drug sensitivity to NB cells.Conclusion DDX1 knockdown can decrease the cell invasion,migration and resistance capability of NB and increase the Doxorubicin and Cisplatin drug sensitivity of NB cells.

Objective To detect the expression of dead box 1(DDX1)gene in tumor tissue and pericarcino-matous tissue of clinical neuroblastoma(NB)samples,and explore the relationship between DDX1 and NB. Methods Five cases of pathological specimens in children with NB were chosen from Department of Pathology,the First Affi-liated Hospital of Xinxiang Medical University between January 2012 and December 2014. In the 5 cases,3 cases were male,2 cases were female,the age of 1 - 5 years old,average age(2. 1 ± 1. 6)years. The NB tissue and pericarcino-matous tissue(pericarcinomatous tissue was normal tissue which was at least 2 cm from the tumor tissue)of 5 children were collected and fixed in 40 g/ L formaldehyde solution. Then with the conventional dehydration,embedding,sectio-ning, dewaxing, hydration, antigen repair, add primary antibodies, secondary antibodies, diaminobenzine chromogenic. The expressions of DDX1 in tumor tissue and pericarcinomatous were observed with light microscopy and with semi - quantitative analysis.(1)Staining degree:no staining with 0 score;light staining with 1 score;medium staining with 2 scores;deeply staining with 3 scores.(2)Positive cells proportion:positive cells proportion ﹤ 10% with 0 score;positive cells proportion within 10% - 30% with 1 score;positive cells proportion within 31% - 60% with 2 scores;positive cells proportion ﹥ 61% with 3 scores. Final scores were a half of the sum of staining degree score and positive cells proportion score,final score within 0 -1. 0 with - ,1. 1 -2. 0 with + ,2. 1 - 3. 0 with + + ,3. 1 -5. 0 with + + + . Results DDX1 were expressed in NB and pericarcinomatous tissues,but visible DDX1 positive staining number more and deeper in NB,DDX1 positive staining number less and light in pericarcinomatous tissues. Five cases of pericarcinomatous tissues immunohistochemical semi - quantitative score were negative and final scores were 1. 0 score or less,the mean value was 0. 5 score. NB immunohistochemical semi - quantitative score were+or + +and final scores were 1. 5 score or higher,the mean value was 1. 8 scores,the expressions of DDX1 were sig-nificantly higher in NB than the pericarcinomatous tissues. Conclusions DDX1 is highly expressed in NB,which may contribute to the development of NB. This suggest DDX1 may serve as an oncogene and play a catalytic role in the de-velopment of NB,which provides a clinical evidence for the follow - up study.

Object To study the anti endotoxic effect of syringic acid (SA) which was isolated from Radix Isatidis (Banlangen in Chinese, BLG). Methods SA was extracted and isolated from BLG and made to 1% solution. The content of SA pretreated ET was quantitatively determined using Limulus Test. Then the ability of fever induction of endotoxin (ET) pretreated with SA was measured using endotoxin induced fever test in rabbits. At last, the lipopolysaccharide (LPS) induced death in mice pretreated with and without SA was compared. Results Being pretreated with SA, 83.16% ET was destroyed, the ET induced fever in rabbits relieved markedly and the LPS induced death rate in mice dropped from 68% to 20%. Conclusion SA isolated from BLG has anti endotoxic effects.

Objective:To investigate the efficacy and safety of dexmedetomidine and dexamethasone in inhibiting opioid-induced cough (OIC) during general anesthesia induction in patients with gynecological tumors.Methods:A total of 180 patients who were scheduled for elective gynecological tumor surgery under general anesthesia in Shanxi Provincial Cancer Hospital from March to November 2019 were selected. They were randomly divided into blank control group, dexmedetomidine group and dexamethasone group according to the random number table method, each group had 60 cases. Firstly, all patients had a 10-minute rest (T 0) after they entered the operate room. Treatment before general anesthesia induction:dexmedetomidine group was pumped dexmedetomidine 0.5 μg/kg (diluted to 10 ml with 0.9% NaCl injection) using an electronic infusion pump; dexamethasone group was injected intravenously dexamethasone 10 mg; blank control group was pumped with 10 ml 0.9% NaCl injection. The pumping was finished within 5 minutes, and the end time of pumping was denoted as T 1. Induction of general anesthesia was performed 5 minutes after the end of pumping: firstly, sufentanil was given intravenously at 0.3 μg/kg, and the injection was finished within 5 seconds (T 2). Two minutes after sufentanil injection (T 3), cis-atracurium 0.3 mg/kg and propofol medium/long-chain injection 2 mg/kg were sequentially injected. Then preoxygenation, endotracheal intubation and mechanical ventilation were implemented in turn. One minute after intubation was recorded as T 4. The incidence and severity of cough in patients within T 2-T 3 of each group were recorded, as well as the incidence of tachycardia, bradycardia, hypertension, hypotension, respiratory depression and myotonia during T 1-T 4. Results:The incidence of OIC in the dexmedetomidine group (10.0%, 6/60) and dexamethasone group (8.3%, 5/60) was lower than that in the blank control group (33.3%, 20/60), and the difference among the three groups was statistically significant ( χ2 = 16.445, P < 0.01), while there was no significant difference in the incidence of OIC between the dexmedetomidine group and the dexamethasone group ( P > 0.05). The incidence of sinus bradycardia in the dexmedetomidine group (16.3%, 10/60) was higher than that in the blank control group (0, 0/60) and dexamethasone group (8.4%, 1/60), and the difference was statistically significant ( P < 0.05). Respiratory depression and myotonia did not occur in the three groups. Conclusions:Pretreatment with dexmedetomidine or intravenous dexamethasone before anesthesia induction can effectively reduce the incidence of OIC in patients with gynecological tumors, and there is no significant difference between the effects of the two drugs. The incidence of sinus bradycardia increases significantly after dexmedetomidine infusion.

Objective To evaluate the relationship between the amount of grafted cells and the incidence of acute graft versus host disease (aGVHD) after haploid hematopoietic stem cell transplantation (haplo-HSCT). Methods Data of 68 patients who underwent haplo-HSCT from Jan 2009 to Dec 2013 were analyzed retrospectively. Influences of different factors on the incidence of Ⅲ-Ⅳ degree of aGVHD after HSCT were evaluated. Results 68 patients including 42 males and 26 females were 5/10-9/10 HLA match with 19 father donors, 24 mother donors, 16 sibling donors and 9 children donors. 51 patients not suffered Ⅲ-Ⅳdegree of aGVHD included 32 males and 19 females with the mean age of 20 years old (5-55 years old). 17 patients sufferedⅢ-Ⅳdegree of aGVHD including 10 males and 7 females with the mean age of 23 years old (5-54 years old). There were no significant differences in the amount of the grafted mononuclear cells (MNC) and CD34+cells, and the white blood cell counts (WBC) and platelet count (Plt) recovered time between two groups (P>0.05). However, MNC number was related to CD34+cell number (P<0.05) and WBC recover time (P<0.05), and the CD34+cells number was related to WBC and Plt recover time (P< 0.05). Conclusion The incidence of Ⅲ-Ⅳ degree of aGVHD is unrelated to the amount of grafted MNC, and CD34+cells.

Interactions between FMDV and cardiac cells are multifaceted and complex ,these interactions leads to pro-teins alterations in cardiac cells inevitably .To understand the pathogenesis of myocarditis after FMDV infection in mice ,the suckling mouse model for myocarditis induced by foot-and-mouth disease virus (FMDV) was established in this study .Suckling mice within 3 days old was selected to infect by FMDV .Myocarditis caused by FMDV in suckling mice was confirmed with clinical symptom monitor .The observation of Hematoxylin and eosin stain (H&E stain) and transmission electron microscopy (TEM) were performed after samples processing .According to conventional polymerase chain reaction (PCR) methods ,prim-ers of VP1 gene was designed ,synthesised and specific FMDV VP1 gene was amplified from the heart muscle of suckling mice . The results indicated that suckling mice appeared low spirit condition ,dyspnea ,and dull reaction within 36 hours after chal-lenge with FMDV .Infiltration of inflammatory cells and dissolution of myocardial fibers were observed with H&E stain and TEM .Special target gene of FMDV was amplified from the heart of infected group .Obvious inflammation in the heart of suck-ling mice caused by FMDV was observed .It's suggested that suckling mouse model for myocarditis induced by FMDV was es-tablished successfully ,which would lay the foundation for researches of myocarditis mechanism in young cloven-hoofed ani-mals .