Atherosclerosis obliterans of lower extremity artery mainly involve the aorto-lliac artery,femoral-popliteal artery and below-knee arteries.At present the major treatment of the disease is endovascular treatment.Due to anatomical differences between the three arteries,the principles and effect of endovascular treatment are different.The endovascular treatment of iliac- femoral lesions,because of its patency rate not lower than surgery,has become the preferred way.On account of the high incidence of femoral- popliteal lesions and the dissatisfaction for patency rate,the endovascular treatment of femoral-popliteal lesions has become a hot research.Endovascular therapy in the below-knee arteries is still in the exploration,currently a small balloon-based.

Identification of seven species of Clematis L. (C. chrysocoma Fr.,C. amendii Fr.,C. fasciculiolia Fr.,C. ranunculoides Fr.,C. rubifolia C. H. Wright,C. florida Thunb. and C. brevicaudata DC. )was studied by RAPD analysis. Results showed that the differences of RAPD between different species were significant,but nonsignificant between same species obtained from different localities

Objective To investigate the risk factors of hyperthermia in early period of an acute cerebral infarction.Methods 299 patients admitted to hospital from January 2003 to December 2004 with a diagnosis of cerebral infartion by CT or MRI,were retrospectively studied.The risks of hyperthermia in the early period for stroke,such as admission conscious level,bulbar palsy,emphysema,atrial fibrillation,multi-cerebral infarction,diabetes mellitus and chronic heart failure were analyzed,Logistic regression analysis was used to determine the factors that were associated with hyperthermia in the early period of stroke.Results 71 patients had fever in early period of an acute cerebral infarction(23.7%).The most common infection was pneumonia(18.4%).The analysis showed that the admission conscious level and atrial fibrillation were the risks of early hyperthermia of an acute cerebral infarction.Conclusion Admission conscious level(subconscious or unconscious/coma) and atrial fibrillation and chronic heart failure are independent risk factors for development of early hyperthermia for acute stroke.

One hundred and fifty cases of gouty arthritis were divided into two groups.Treatment group(85 cases) were treated with Tong Feng lotion and control group(65 cases)with external application of Voltaren. Results indicated that Tong Feng lotion could improve clinical symptoms and function of joints, and decrease blood sedimentation,reaction protien C,blood uric acid and blood viscosity; the total effective rate was 89.42% in the treatment group and 75.38% in the control group, with significant difference between the two groups(P<0.05); there was no obvious relation between therapeutic effects and TCM syndrome types.

Objective:To study the change of Neuropeptide Y (NPY) plasma and its clinical si gnificance and the effects of Astragalus on NPY in plasma in chronic renal fail ure patients.Methods:Radioimmunoassay was used to measure plasma concentration of NPY.Results:①The plasma concentration of NPY in CRF patients was significantly high er than tha t in healthy group,and the plasma concentration of NPY in uremia patients was si gnificantly higher than that in nitremia patients.②The plasma concentration of NPY was positively correlated with the serum value of CRE and the level of MAP in CRF patients.③After Astraglus treatment for 3 weeks,the level of NPY in plas ma was significantly decreased in experiment group,but in control group,there wa s no significant change.Conclusion:The disturbance in synthesis,secretion or metabolism of NPY occurred in CRF patients,which significantly correlates with the occurrence and development of CRF.Astraglus can influence the NPY in plasma in CRF patients,so as to improve the renal function.

Objective Analysis of the characteristic preferences of high-level medical care talents to identify effective and targeted incentive plans for them.Methods Questionnaires were designed in view of characteristics of these talents for investigation in a tertiary hospital in Nanjng.The results were summarized in the main factor analysis method.Results Characteristic preferences as found in the summary are three main factors with distinctive significance,which are material needs,achievement needs and development needs,ranking 4.7 points,3.8 points and 3.9 points respectively.Conclusions These three characteristic preferences could be.sorted by ranking as achievement needs,material needs and development needs.Based on which,the corresponding incentive countermeasures are proposed.

Object To study the separation effect of three macoroporous resins for lycopene and ? carotene, in order to optimize the suitable one. Methods The performances of adsorption and desorption for lycopene and ? carotene with three macroporous resins D4020, X 5 and AB 8 were compared, and adsorption dynamics process of resins was studied. Results The adsorptive capacities of X 5 resin for lycopene and ? carotene are the most, they are 7 44 and 11 2 mg/g, respectively. Desorption ratio of X 5 resin is the highest. Conclusion X 5 resin is an ideal adsorbent for lycopene and ? carotene extraction.

The Acetobacter xylinum and Schizosacchromyces sp. were isolated from tea fungus culture. Each strain 's function on the production of antibacterial protein was explored by single-strain culture and double-strain culture under various condition. The preliminary conclusion is that the protein is synthesized by the yeast cells and its antibacterial activity is significantly improved through modification by bacteria enzymes. The results of co-cultures of several yeasts with the Acetobacter xylinum show that Schizosacchromyces is superior to other yeasts.

Objective To evaluate the effect of intrathecal resveratrol on activation of Ca2+/ calmodulin-dependent protein kinase Ⅱ (CaMK Ⅱ) in spinal dorsal horn neurons of rats with bone cancer pain.Methods Thirty-two adult female Sprague-Dawley rats,weighing 180-220 g,were equally randomized into 4 groups using a random number table:sham operation group (group S),bone cancer pain group (B group),and bone cancer pain + solvent control group (BD group).Walker 256 mammary gland cancer cell suspension (4× 105 cells/ml) 5 μl was injected into the medullary cavity of the right tibia in B,BR and BD groups.Normal saline 5 μl was given in group S.On 12,13 and 14 days after injection of mammary gland cancer cells,resveratrol 200 μg/10 μl was injected intrathecally once a day in group BR,and 1％ dimethyl sulfoxide 10 μl was intrathecally injected once a day in group BD.Before injection of mammary gland cancer cells (T0) and on 3,5,7,10,12 and 14 days after injection of mammary gland cancer cells (T1-6),mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured.The rats were then sacrificed and L4,5 segments of the spinal cord were removed for confirmation of the location of phosphorylated CaMK Ⅱ (p-CaMK Ⅱ) in spinal dorsal horn neurons (by immunofluorescence) and for detection of p-CaMK lⅡ expression (using Western blot).Results Compared with group S,MWT and TWL were significantly decreased at T2-6,and p-CaMK Ⅱ expression was upregulated at T6,and p-CaMK Ⅱ was mainly co-expressed with neurons in B,BR and BD groups.Compared with group B,MWT and TWL were significantly increased at T5,6,and p-CaMK Ⅱ expression was down-regulated at T6 in group BR.There was no significant difference in MWT,TWL,and p-CaMK Ⅱ expression at each time point between group B and group BD.Conclusion Resveratrol can alleviate hyperalgesia in rats with bone cancer pain and inhibited activation of CaMK Ⅱ in spinal dorsal horn neurons may be involved in the mechanism.

Objective To investigate the expression of astrocyte elevated gene 1(AEG-1) in non-small cell lung cancer(NSCLC) and its clinicalpathological significance .Methods The expression of AEG-1 in NSCLC tissues and adjacent normal lung tissues was detected by RT-PCR and Western blot .The expression of AEG-1 in 87 NSCLC samples and 54 non-cancerous lung tissues was ex-amined with immunohistochemistry .Results RT-PCR and Western blot indicated that AEG-1 was more expressed in NSCLC tis-sues compared with adjacent normal lung tissues .The positive rate of AEG-1 in NSCLC tissues was 52 .9% ,which was significantly higher than that in non-cancerous lung tissues .The difference between the two groups was significant (P=0 .007) .Relevance was analyzed between the expression of AEG-1 and clinicalpathological characteristic .The expression of AEG-1 was positively correlated with T stage and N stage(P<0 .05) .Conclusion AEG-1 was specifically up-regulated in NSCLC tissues compared with non-can-cerous lung tissues ,suggesting that AEG-1 may play an important role in tumor development and progression ,and could be identi-fied as a biomarker for diagnosis of NSCLC .