AIM: To evaluate the effect of cold acclimation on activity and concentration of UCP in mitochondria inner membrane of rat brown fat. METHODS: Using -labeled GTP binded UCP in mitochondria, radioactivity was measured by liquid aglimmer instrument, the dissociation constand(Kd) and maximal binding(Bmax) were determined by Scatchard plot. RESULTS: In cold acclimation pretreated rats, brown fat weight increased, Bmax increased 1.9-fold,Kd decreased 1.4-fold compared with control. CONCLUSION: Cold acclimation not only increase concentration of UCP in mitochondria inner membrane of rat brown fat,but also increase its activity.

Objective To design and synthesize schisandrone derivatives for the therapy of disease like AD,and to meas-ure their anti-oxidation activity.Methods Ten compounds were synthesized,and seven of them were first reported.The influ-ence all the compounds on SOD from the mouse serum were measured to evaluate anti-oxidation activity.Structure-activity rela-tionship(SARs)were also discussed.Results Compounds 2,3,5 and 8 could increase the activity of SOD in mouse serum.Fur-thermore,Compounds 2,3 and 5 showed better activities than schisandrone.Conclusion Synthesized schisandrone derivatives showed anti-oxidation activity.The SARs demonstrated the carbonyl of C-1 position and the methyls of C-2 and 3 had no influ-ences to the anti-oxidant activity.