Objective To explore the clinical therapeutic effect of combined treatment of low molecular weight heparin calcium and breviscapine in elder pulmonary heart disease .Methods 303 patients in elder pulmonary heart disease in the hospital from May 2011 to June 2013 were divided into three groups .100 patients in group A were received the routine therepy ,102 patients of group B were received routine therapy combined with low molecular weight heparin calcium only ,101 patients of group C were received routine therapy combined with low molecular weight heparin calcium and breviscapine .the changes of clinical symptom ,blood gas a-nalysis ,hemorheology in groups before and after treatment were observed .Results The effective rates of group A ,B ,C were 59 .0% ,73 .5% and 90 .0% ,the results showed that the blood gas analysis and hemorheology parameter in groups B and C had more improved than group A (P<0 .05) ,and better in group C than group B(P<0 .05) .Conclusion The combined treatment of low molecular weight heparin calcium and breviscapine can decrease the press of lung artery ,relieve the bear of right ventricle and improve the arterial partial pressure of oxygen ,mean pulmonary arterial pressure and hemorheology parameter ,and has less adverse reaction .

Objective To investigate the effect of low molecular weight heparin calcium combined with breviscapini in treating a-cute exacerbation of elderly chronic pulmonary heart disease and its mechanism.Methods 86 patients with acute exacerbation of elderly chronic pulmonary heart disease in our hospital from October 2011 to June 2013 were randomly divided into two groups. There were no significant differences in age,gender and disease condition between the two groups.44 cases were in the therapy group and the other 42 cases were in the control group.Both two groups received the routine therepy.On this basis the therapy group was added with low molecular weight heparin calcium and breviscapini.Then the improvement of clinical symptoms and the changes of plasma nitric oxide (NO)and endothelin-l (ET-1)after treatment were observed in the two groups.Results The total effective rates in the therapy group and the control group were 90.1%and 71.4% respectively;the levels of plasma NO and ET-1 after treatment in the two groups were significantly improved,but the improvement in the therapy group was better than that in the control group with the statistical difference between them(P <0.05).Conclusion Low molecular weight heparin calcium combined with breviscapini can significantly improve the symptoms in the ederly pulmonary heart disease,decrease the lung arterial press,re-lieve the load of right ventricle and affect the levels of plasma NO and ET-1 with less adverse reactions.

Objective To investigate the relationship between myeloperoxidase(MPO) and ankle brachial index(ABI) in patients with htypehypertension.Methods A total of 133 patients were enrolled in this study,and divided in to htypehypertention group(n=72),non-htypehypertension group(n=30) and normotension group(n=31).Their Hcy,ABI and MPO levels were measured and compared.Results The ABI in htypehypertention group and non-htypehypertension was significantly lower than that in normotension group (P<0.05),ABI in non-htypehypertension group was lower than that of htypehypertonsion group(P<0.05).MPO and CRP in the htypehypertension group was significant higher that those of normotension group(P<0.05).The ABI was negatively related with the serum MPO and CRP levels in htypehypertention group (r=-0.712,-0.722,P<0.05).Conclusion ABI is closely related with MPO,and play an important role in diagnosis of artery function impairment and high risk of htypehypertension patients.

HOXA10 gene plays an essential role in differentiation of the endometrium and in human reproduction. The aim of this study was to investigate the regulatory effect of sex steroids and HB-EGF on HOXA10 gene in Ishikawa cells. Ishikawa cells were incubated with 17-beta estradiol (10(-8) mol/L), medroxyprogesterone acetate (MPA) (10(-6) mol/L), RU486 (10(-5) mol/L) or HB-EGF (10 ng/mL) for 48 h respectively. The expression of HOXA10 gene was detected by immunofluorescence, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting. Our results showed that either estrogen alone, progestin alone or progestin combined with estrogen could significantly increase the expression of HOXA10 gene 48 h after the treatment (P<0.05). But estrogen combined with progestin and RU486 could inhibit the up-regulation by estrogen and progestin. HB-EGF could elevate the expression of HOXA10 gene 48 h after the treatment (P<0.05). It is concluded that both estrogen and progestin can up-regulate the expression of HOXA10 gene in Ishikawa cells, but RU486 can inhibit the effect and HB-EGF can elevate the expression level of HOXA10 gene.
Cell Line, Tumor ; Endometrial Neoplasms/*pathology ; Estradiol/*pharmacology ; Genes, Homeobox ; Homeodomain Proteins/genetics ; Homeodomain Proteins/*metabolism ; Intercellular Signaling Peptides and Proteins/*pharmacology ; Medroxyprogesterone Acetate/*pharmacology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism

Objective To explore the correlations between Annexin A1 protein expression and clinicopathological character-istics in carcinoma of papillary thyroid.Methods The different expressions of annexin A1 in papillary thyroid tissue and para-cari-noma tissue were investigated by immunohistochemistry.Results Among 69 samples tissues of papillary thyriod carcinoma,the positive rate of annexin A1 was higher than that of 69 para-carcinoma tissues(88.41%vs .8.69%),there was a significant difference (P <0.05).Furthermore,the expression of annexin A1 was correlation with the lymph node metastasis and tumor size,which was higher in ≥1 cm diameter of tumor(P <0.05).Conclusion High AnnexinA1 positive expression in papillary thyroid cancer tissues is associated with tumor malignant progression,which might be a valuable predictor and potential target for the diagnosis and treat-ment of papillary thyroid carcinoma.

Objective To investigate the homology of methicillin-resistant Stphylococcusaureus(MRSA)from the neonatal intensive care unit(NICU)of a children's hospital,and evaluate routes and preventive strategies of MRSA healthcare-associated infection(HAI). Methods MRSA strains from neonates and environment of NICU between October and December 2014 were collected,and strains were identified by VITEK-2 microbial analysis system and cefoxitin Kirby-Bauer method,homology of MRSA was analyzed by pulsed-field gel electrophoresis (PFGE ). Results A total of 6 MRSA strains were isolated from NICU between October and December 2014,3 of which (bed-58,70,and 100)were detected MRSA from specimens,MRSA were isolated from neonatal incubator and nurse (nasal swabs and hands)who cared for neonate at bed 58. 5 of 6 MRSA strains were homology,antimicrobial susceptibility testing result showed that No. 1-5 strains were resistant to clindamycin and amoxicillin/clavulanic acid,No. 6 strain was slightly different from No. 1-5 strains,No. 6 strain was susceptible to both clindamycin and amoxicillin/clavulanic acid. PFGE results showed that No. 1-5 strains were of the same type,No. 6 strain was a different type. Conclusion The main route of this MRSA transmission is contact transmission,especially through the hands of health care workers,identification and analysis of epidemic strains by PFGE technique is an effective measures to prevent HAI outbreak and perform epidemiological study.

Objective To investigate the effects of Chinese herbal medication combined with nutrition intervention on perioperative nutrition in patients with colon carcinoma.Methods Ninety-six patients with colon carcinoma were divided according to their odd or even number into the control group and the intervention group.The former were given routine nutrition and the latter Chinese herbal medication combined with nutrition intervention.Then the mini-nutritional assessment(MNA)was used to assess the changes before and after operation and the complications.Results The intervention group was significantly better than the control one in terms of all nutrition factors but hematoglobin(P<0.05).The incidences of complication and dystrophy in the intervention group were lower than in control group(P<0.05).Conclusion The application of Chinese herbal medication combined with nursing intervention is important for the improvement of their nutrition and the reduction of postoperative complications.

ObjectiveTo comprehend the similarities and differences of postoperative quality of life between mastectomie avec dissection axillaire and modified radical mastectomy among patients with breast cancer,and in order to supply references for establishing corresponding nursing measures. Methods160patients with early breast cancer were selected,40 patients accepted the mastectomie avec dissection axillaire (the conservative group)and 120 patients accepted modified radical mastectomy (the radical group).The quality of life of patients was surveyed with questionnaires. ResultsThe EORTC QLQ-C30 score of the conservative group was not significant than the radical group.The EORTC QLQ-BR 23 score showed that the conservative group had a better self-evaluation of their configuration. ConclusionsThe patients who accepted the mastectomie avec dissection axillaire will live a better life than those who accepted modified radical mastectomy.

Objectives To investigate the clinical characteristics and diagnosis of Gaucher disease. Methods The clini-cal features, enzyme activities and genetypes of an infant with Gaucher disease II were analyzed. Results The main clinical manifestations of the infant included hepatosplenomegaly, ocular dyscinesia and mental retardation. Gaucher′s cells could be seen in the bone marrow aspirates. Glucocerebrosidase activity was low (0.3 nmol/g/min). Serum chitotriosidase activity was high (87317 nmol/L/min). GBA mutations were M85T (c.371T>C) and R120W (c.475C>T). Conlusions Main features of Gau-cher disease II are hepatosplenomegaly with nerve system injury. Glucocerebrosidase activity and gene analysis are important for the diagnosis of Gaucher disease.

Objective To investigate the clinical manifestations and MR imaging features of olivopontocerebellar atrophy(OPCA).Methods The clinical data and MR findings of 8 patients with OPCA (OPCA group) were retrospectively analyzed,and 75 cases of healthy physical examination people were chosen as control group (they were divided into 21-30 years group,31-40 years group,41-50 years group,51-60 years group,61-70 years group,and 15 cases in each group),all subjects under went pons measurement.Results All 8 patients with OPCA were sporadic cases.The cerebellar symptom,autonomic symptoms and extrapyramidal symptoms were common manifestations.The head MR imaging of OPCA presented with cerebellum and brain stem atrophy,cross sign was the characteristic appearance.Vertical diameter and anteroposterior diameter of the pons in OPCA group patients was (20.63 ± 2.16) and (16.74 ±4.37) mm,compared with control group [(27.14 ±1.73) and (22.28 ± 1.49) mm],there was significant difference (P ＜ 0.01).In control group,vertical diameter and anteroposterior diameter of the pons maximum in 21-30 years group and 31-40 years group,increased with age,the value had been reduced,but there was no significant difference (P ＞ 0.05).Conclusion MR is the first choice for the diagnosis of OPCA,combine with clinical manifestations may improve the diagnostic accuracy.