目的探讨健身秧歌舞对女性更年期综合征的缓解作用。方法对60名有更年期综合征症状的女性实施12周健身秧歌舞练习,分析受试者练习前后血清雌性激素水平及更年期综合征症状的变化。结果受试者血清雌二醇、孕酮含量提高(P<0.05),睾酮含量明显降低(P<0.01),更年期症状阳性率降低(P<0.05)。结论健身秧歌舞能有效缓解女性更年期综合征症状。

Objective To study characteristics of clinical manifestations of patients with neuropsythiatric systemic lupus erythematosus(NPSEL). Methods From Jan.2000 to Oct.2004,the symptoms、signs and results of assistant check of 36 patients with NPSEL were analysed and summarized. Results 9 kinds of symptoms are complained,in which headache、weakness and disorder of consciousness were most frequent;13 kinds of signs were found,in which pathologic reflex、disorder of consciousness and limb paralysis were most frequent. Conclusion (1)NPSEL is a kind of manifestation of active SEL.(2)No matter how long disease course of SEL may be,the patients can be involved by NPSEL.(3)Manifestations of NPSEL is complicated.

Objective: To explore the characteristics of control response and sustained attention in children with chronic tic disorders (CTD). Methods: 38 children fulfilled DSM-Ⅳ diagnosis of CTD and 38 normal children are involved in the study. All participants were assessed with the integrated visual and auditory continuous performance test (IVA-CPT). The differences of sustained attention and control response were compared. Results: Most of the values of IVA-CPT were lower in children with CTD compared with normal children. There was no significant difference between the visual and auditory sustained attention and control response in children with CTD. Conclusion: The present study suggests that control response and sustained attention are both impaired in children with CTD.

Objective To evaluate the clinical efficacy of cefotaxime combined with gamma globulin on neonatal septicemia. Methods The subjects of this study were selected from 88 cases of neonatal septicemia admitted in Jiangyou No.903 hospital from April 2014 to April 2017. They were randomly divided into two groups, each with 44 cases. The control group was given symptomatic treatment and cefotaxime, while the observation group symptomatic treatment, cefotaxime and gamma globulin as well. After 7days of treatment, the overall effective rates, case fatality rates, clinical symptoms (body temperature, resistance to milk, neurological symptoms), time for improvement and hospital stay were compared between the two groups. Results The overall effective rate of the observation group was 95.45％, much higher than 72.73％ of the control group (P<0.01). The observation group had much lower case fatality rate than the control group did (2.27％ vs 18.19％) (P<0.05). The time the observation group took for improvement in the condition of body temperature, resistance to milk, neurological symptoms, and hospital stay was (3.25±1.07) days, (4.93±1.96) days, (5.92±1.58) days, and (6.80±1.94) days respectively, all significantly shorter than the time the control group took (P<0.01). Conclusion The combination of cefotaxime and gamma globulin is effective in the treatment of neonatal septicemia. It can significantly reduce case fatality rate and shorten the time for clinical symptoms and hospital stay.

Objective: To evaluate the therapeutic effect of combining use of acupuncture and drugs on rheumatoid arthritis(RA) in the early and the active stages of by the imaging of bone and joint with 99m TC-methlene diphosphate(MDP). Methods: 102 cases of RA in the early and the active stages were randomly allocated into the compound Fengshining group (Group A),acupuncture group(Group B), and compound Fengshining plus acupuncture group(Group C).They were treated for 30 days. All patients were examined before treatment and after treatment by 99m TC-MDP imaging of the whole body, hands and feet, and analyzing method of random interested region was used to count the ratio of radioactivity in the affected area of RA to radioactivity in adjacent normal bone(T/TN). Meanwhile, joint swelling index, joint tenderness index, morning stiffness, gripping force, and erythrocyte sedimentation rate (ESR) were also observed. Results: T/TN of the Group A after treatments had no significant difference comparing with that before treatment(t = 1.35, P＞0.05), while the Group B and C had significant differences(t = 5.31, t=8.97, P＜0.01). The changes of clinical and experimental examination in the Group C had more significant differences than those in the Group A and B(P＜0.05).Conclusion: Acupuncture therapy could promote RA recovery, and combining use of acupuncture and compound Fengshining had better effect.

Objective To observe stimulation and regulation of T lymphocyte subtypes by wild-type and mutant A?40 and A?42 conjugated with different adjuvants and to investigate possible ways to lower the toxicity of A? immune response. Methods Forty BALB/c mice were randomized into five groups:Al adjuvant group,A?42+CFA group,A?42+Al group,A?40+Al group and A?40(E22A)+Al group. After an initial immunization and booster immunization,antibody was titrated. Their splenocytes were stimulated by respective antigen. After 48 h,the quantities of IFN-?,IL-2,TNF-? and IL-4 were tested. After 72 h of culture,the proliferative response of splenocytes was detected by CCK-8 assay.Results Experimental groups generated specific anti-A? antibodies with proliferative response of spleen lymphocytes. Cytokine detection in culture supernatant showed that A?42+CFA group had the highest secretion of Th1-type cytokines IFN-?,IL-2,TNF-? when A?40(E22A)+Al group had the lowest(P

Objective To further study on the chemical constituents in the peels of Citrus changshan-huyou.Methods The chemical constituents were separated by repeated silica gel column chromatography,the structures were determined by spectral analyses.Results Ten compounds were obtained and identified as huyoujiasu(2'-hydroxy-malonyl-3,4-dihydroxybenzoic acid,Ⅰ),3'-hydroxy-4',5,6,7,8-pentamethoxyflavone(Ⅱ),eriodictyol(Ⅲ),tetracosanoic acid(Ⅳ),glyceryl dodecanoate(Ⅴ),neoeriocitrin(Ⅵ),naringin(Ⅶ),neohesperidin(Ⅷ),4-methoxy-3-hydroxybenzoic acid(Ⅸ),and 3,4-dihydroxybenzoic acid(Ⅹ).Conclusion 2'-hydroxy-malonyl-3,4-dihydroxybenzoic acid is a new compound and the above ten compounds are obtained from this plant for the first time.

Objective:To study the chemical constituents of the peels collected from Citrus changshan-huyou Y.B.Chang,and further screen the bioactive components as the lead structures.Methods: These compounds were isolated by repeated flash column chromatography on silica gel and Sephadex LH-20.The structures of isolated compounds were elucidated by using IR,EIMS,and NMR analyses.Results: One novel compound along with nine known compounds were obtained and identified as huyoujiasu(Ⅰ),3-hydroxy-4-methoxybenzoic acid(Ⅱ),3,4-dihydroxybenzoic acid(Ⅲ),fatty acid(Ⅳ),gly-ceride(Ⅴ),6,7-dimethoxycoumarin(Ⅵ),6′,7′-dihydroxybergamottin(Ⅶ),daucossterol A(Ⅷ),daucossterol B(Ⅸ) and huyou-triterpenoid(Ⅹ).Conclusion: One new compound,Huyoujiasu,was obtained from these peels by repeated column chromatography,and the other known compounds were isolated from this fruit for the first time as well.

Objective: To investigate the action and mechanism of Bupleurum chinense SD and Schisandra chinensis Turcz extract on liver fibrosis in rat.Methods: The model was prepared by injection of 40% CCl 4 plant oil solution, then the enzyme active AST、AST、MAO、ALP、r-GT、TG in serum and MAO、ALP、GSH、TG in hemogeneous were measured by enzyme method, the level of Pc-Ⅰand PcⅢ were measured by radio-immunity. Results: Bupleurum chinense and Schisandia chinensis extract can reduce significantly enzyme active of ALT、AST、TG、MAO in serum of fibrosis rat, the GSH was elevated and the r-GT was not effect in serum, but it could reduce the level of MAO、ALP、Pc-Ⅰ、Pc-Ⅲ in the homogeneous, sometime the TG was increased and the GSH was not effect in homogeneous. The histopathology showed that Bupleurum chinense and Schisandia chineusis extract could get damaged liver cell back and reinforce the regeneration of hepatic cell. Conclusion:Bupleurum chinense and Schisandid chinensis extract the action of protective and restoration on injured liver cell, and its could eliminate the bile accumulation, to retard the fatty degeneration in fibrotie rat.

Objective: To analyse the behavioral problems of children with attention deficit hyperactivity disorder.Methods: 70 boys fulfilled ADHD criteria of DSM-IV were further divided into 3 subtypes: predominantly inattentive(PI,44 cases),predominantly hyperactive-impulsive(HI,6 cases) and combined type(CT,20 cases).Compare the behavioral problems of subtypes of ADHD and 63 normal children.Results: The incidence of behavioral problems in boys with ADHD(48.57%) was significantly higher than that in control group(12.70%),and significant differences in incidence of behavioral problems were found among the three subtypes of ADHD.ADHD CT subtype had the highest rate of behavioral problems.PI subtype had more fissility than HI;CT and HI subtypes had more aggressive behaviors,and HI had more delinquence than PI and CT.Conclusion: Boys with ADHD had more behavioral problems than normal ones.CT subtype had the highest rate.The characteristics of behaviors were different among three subtypes.