Objective:To prepare anti-HBV S protein hybridoma cell line,this will be helpful to the establishment of rapid HBV infection diagnosis assay.Methods:BALB/c mice were immunized with the recombinant Hepatitis B vaccine.A hybridoma cell line which consistently secreted monoclonal antibody(McAb)against HBsAg was obtained through cell fusion.The specificity of the McAb was analysed by indirect ELISA and Western blot analysis.The immunoglobulin(Ig)subtypethe ascites titers, and the affinity of the obtained mAbs were determined by indirect ELISA.Results:From over hundred positive hybridomas which secreted anti-S protein mAbs,one of hybridomas was screened out,designated 4D2.The subtype of the McAb was lgG_1. The titer of 4D2 mAb produced by ascites fluid was over 1:10~6.The relative affinity constant of 4D2 mAb was determined as 10~5.Conclusion:One of high titer,specific mAb against HBV S protein has been successfully prepared and primarily identified,which may be useful in the development of a rapid and convenient diagnostic kit for detection of HBV infection.

The traditional SPECT has been considered unable to quantitatively analyze due to the limitations of device performance and reconstructing algorithm. Because of the development of SPECT devices, the emergence of SPECT/CT, and the improvement of reconstructive algorithms, SPECT can be used for quantitative analysis like PET. The current quantitative SPECT researches focus on the usefulness of quantitative SPECT technology in clinical diagnosis and the guidance and evaluation of radionuclide therapy. This review summarizes the development of SPECT quantification, the current status of quantitative SPECT researches in clinical diagnosis and radionuclide therapy, as well as the limitations and advantages of SPECT quantification.

Objective to study the effect of curcumin on tumor necrosis factor α(tNF-α)-induced osteoblast apoptosis. Methods Primary osteo-blast cells were divided into three groups:control group(no treated),group treated with tNF-α(treated with tNF-α for 24 h),and group pretreated with curcumin(treated with curcumin for 2 h,and then treated with tNF-α for 24 h). Western blot analysis was carried out with primary antibodies against caspase-2,caspase-3,caspase-8,Bcl-2,Fas,and tRAIL. Apoptosis cells were observed by immunofluorescence microscope. Results In group treated with tNF-α,the protein levels of caspase-2,caspase-3,caspase-8,Fas,and tRAIL increased(P < 0.01),whereas the protein level of Bcl-2 remained unchanged(P > 0.05). In group pretreated with curcumin,the protein levels of caspase-2,caspase-3,caspase-8,Fas,and tRAIL were lower than those in group treated with tNF-α(P < 0.01). the number of apoptotic cells was dramatically increased after the administration of tNF-α. While the number of apoptotic cells decreased in the presence of curcumin. Conclusion the present study clearly demonstrated that cur-cumin can suppress osteoblast apoptosis induced by tNF-α.

Objective To study the expressions of Toll-like receptor (TLR)2 and TLR4 in colonic mucosa of patients with irritable bowel syndrome (IBS) and in normal subjects. Methods Thirty patients with diarrhea predominant IBS and 12 healthy volunteers were enrolled. The expressions of TLR2 and TLR4 in colonic mucosa were examined by immunohistochemistry (IHC).TLR2 and TLR4 were semi-quantitative analyzed with average absorbence. Results Contrast to healthy controls, the lamina propria of IBS patients showed edema and looseness with lots of inflammatory cells infiltration. There was no difference in expression of TLR2 between healthy controls and IBS patients (P>0.05). Compared with healthy controls, TLR2 in crypt epithelium and TLR4 in luminal surface of IBS patients were significantly up-regulated (TLR2 : 6.7 % vs. 50.0 %,TLR4: 40.0% vs. 0, P<0. 05). The TLR4 expressed in intestine epithelial cell of both the apical surface and the basolateral surface in 86.7% of patients with IBS, and in 50% of healthy controls.The positive cells of TLR4 in lamina propria were higher in patients with IBS than those in healthy controls (70. 084 ± 21. 887 vs. 20. 577 ± 4. 546, P<0.01). The A values of TLR2 and TLR4 in colonic mucosa of the patients with IBS were higher than those in healthy controls (TLR2:0. 3079±0. 0283vs. 0.3886±0. 0510,TLR4:0. 3044±0. 0481 vs. 0. 3971 ±:010996,P<0. 01). Conclusions Inflammatory cells infiltrated into colonic mueosa in patients with IBS suggested that inflammation might participate in the pathogenesis of IBS. Up-regulated expressions of TLR2 and TLR4 in IBS patients supposed that they might contribute to the occurrence of IBS.

ObjectiveTo study the change of serum interferon- γ(IFN- γ ) and interleukin-4(IL-4)level in children suffered from cough variant asthma after montelukast combined with loratadine therapy.MethodsForty-six patients of children cough variant asthma were recruited and accepted montelukast combined with loratadine therapy for 8 weeks.The changes of serum IFN-γ and IL-4 before and after therapy were compared.ResultsCompared with before therapy,the level of serum IFN- γwas decreased [ (59.94 ± 9.43) ng/L vs.(39.94 ± 9.07) ng/L ] and IL-4 was increased[ ( 48.78 ± 22.23 ) ng/L vs.(60.78 ±16.48) ng/L] after therapy,there were significant differences (P ＜0.05).ConclusionMontelukast combined with loratadine in the treatment of children cough variant asthma can improve the balance of IFN- γ/IL-4,which deserve further study.

Objective To investigate the situation and development trend of adolescent mental health diathesis, and explore the differences and influencing factors among students with different features.Methods 338 adolescents were tested with the Adolescent Mental Health Diathesis Scale. Results ①Except the self levels,there was significant differences in other levels and sublevels of the mental health diathesis between college( ( adaption(64.17 ±7.12) interpersonal(58.70 ±7.13 ) personality( 127.72 ± 14.41 ) motive power system( 109.34 ±11.76) cognitive style(43.76 ±4.24) copying style( 106.14 ± 10.33 ) ) and middle school students( ( adaption (68.27 ±5.86) interpersonal(64.78 ±5.25) personality( 135.72 ± 10.52) motive power system( 129.79 ±10.12) cognitive style(49.86 ± 4.70 ) copying style ( 124.77 ± 9.80 ) ), boys and girls, students in cities and countries, with different levels of parents'education or economic of family.②Correlation and reegression analysis showed that the form and development trend of mental health diathesis was influenced by several factors.Conclusion In general, the state of adolescent mental health diathesis is good in Jinan.Different groups of adolescents have diverse styles on the scales and dimensions of mental health diathesis.

Objective To study the expressions of TLR4, CD14, MD-2 and NF-kB in colonic mucosa in patients with diarrhea-irritable bowel syndrome (IBS-D) , and compared with normal subjects. The purpose of this study is to explore the role of TLR4 and TLR4 signal transduction pathway in the pathogenesis of IBS-D. Methods The expressions of TLR4, CD14, MD-2 and NF-kB in colon mucosa were examined by immunohistochemistry (IHC) in 30 IBS-D patients and 12 healthy volunteers separately. The average absorbance (A value) of TLR4 was analyzed. The positive expression rates of CD14, MD-2 and NF-kB of colonic mucosa were studied. Results Compared with healthy controls, significant upregulation of TLR4 expression relative to controls was found in colon mucosa of IBS-D. A value of TLR4 in IBS-D was significantly higher (0.3971 ±0.0996 vs 0. 3044 ±0.0481). The positive rate and intensity of NF-kB in IBS-D were significantly higher than those in healthy. The number of positive cells of MD-2 showed significant increase in lamina propria of IBS-D against controls. The percent of CD14 positive was upregulated in lamina propria in IBS-D. The expressions of MD-2 and CD14 in intestine epithelial cell were low or negative. Conclusions There is the activation of the signal transduction pathway of TLR4/NF-kB in the colonic mucosa of patients with IBS-D. Up-regulated expression of TLR4 in IBS patients suppose that it might contribute to occurrence of IBS-D.

Multi-drug resistance (MDR) of breast cancer is a key factor of breast cancer treatment failure.MicroRNA (miRNA) is a class of endogenous expressed,small,single-stranded RNA molecules,and regulate the expression of genes at post-transcriptional level by combining with target message RNA.MiRNA participate in the mechanisms of drug resistance of breast cancer,and are feasible therapeutic targets for the treatment of drug resistance in breast cancer.Finding and studying miRNA related with drug resistance of breast cancer as well as their mechanisms have become a current research hotspot.

Based on the kinetics of alcohol metabolism in animal models,the mathematicalmode for studing of the alcohol kinetics in person of the acute alcohol poisoningwas established.This mathematical mode can be expressed as x(t)=x_0(1-e~(-kat))-k_bt,which allows calculating the absorpion constant (k_a) arld breakdown rate constant(k_b) for alcohol the curve in dynamic change of the alcohol concentration followingadministration of a large amount of alcohal can be obtained the amount of alcoholin human body [x,(t)] after drinking can be checked out.from the urine Theexperimental study in monkeys showed that the parameters derived from the mathe-matical mode agued witk the results obtained from the experiments

Objective To study and analyze the effect of psychological intervention combined with glucocorticoid on the treatment of nephrotic syndrome and the quality of life in patients with nephrotic syndrome. Methods 100 patients with nephrotic syndrome treated in he affiliated hospital of Hangzhou normal university from February 2015 to August 2016 were selected and randomly divided into the control group and the experimental group, with 50 patients in each group. Patients in the control group received basic treatment, including trial and treatment, and diuretics, and were treated with corticosteroids on this basis. Patients in the experimental group on the basis of the control group, psychological intervention combined with glucocorticoid treatment, pay attention to the psychological status of patients and patients, strengthen communication and exchanges, increase confidence in the treatment and the treatment compliance of patients. The quality of life and treatment effect of the experimental group and the control group were compared and analyzed. Results After the corresponding treatment, the daily score of the patients in the experimental group was (15.19±3.19) points, and the daily life score of the control group was (22.21±5.19) points (QOL). The QOL score of the experimental group was significantly lower than that of the control group, and the quality of life was significantly higher than that of the control group, with statistical difference (P<0.05). The effective rate of the experimental group (90.0％) was significantly higher than that of the control group (66.0％), with statistical difference (P<0.05). Conclusion Psychological intervention combined with glucocorticoid on the treatment of nephrotic syndrome, the clinical effect is better, which can greatly improve the quality of life, and is worthy of promotion.