Multi-drug resistance (MDR) of breast cancer is a key factor of breast cancer treatment failure.MicroRNA (miRNA) is a class of endogenous expressed,small,single-stranded RNA molecules,and regulate the expression of genes at post-transcriptional level by combining with target message RNA.MiRNA participate in the mechanisms of drug resistance of breast cancer,and are feasible therapeutic targets for the treatment of drug resistance in breast cancer.Finding and studying miRNA related with drug resistance of breast cancer as well as their mechanisms have become a current research hotspot.

It has widely spread thta critical li lness is characterizedas a sat te of immunosuppression and inflammation,and patients are unable to provide their own nourishment.So it has lead to the ed velopment of un tritional support por ducts or interventions designed to enhance the host immune response and/or suppress the inflammation.But there′s still a lot of controversy about immunonutrition in critically ill patients.This pa-per reviewed the research status of immunonutrition and nutritional support products.

OBJECTIVE:To prepare gatifloxacin chitosan eye drops and to establish its quality control.METHODS:The eye drops was prepared with chitosan as the base.The content of gatifloxacin was determined by UV spectrophotometry and the stability of the preparation was detected by initial average accelerated method.RESULTS:The linear range for gati?floxacin was5.0～15.0?g/ml(r=0.9990,n=5),the average recovery rate was98.91%(RSD=1.43%).The activation energy for pyrolytic reaction of gatifloxacin chitosan eye drops was25.91kcal/mol,its shelflife at25℃and10℃were93.8days and950days,respectively.CONCLUSION:The preparation technology is feasible and the quality control method is reliable.

1 000 yuan were 68.84%,14.34%,14.35%,2.48%,respectively.In terms of percentages of the categories at the levels above 100 yuan,leading the list were anti-neoplastic drugs,followed in descending order by drugs used for hematological system,anti-neoplastic adjuvants,biological response modifiers,anti-fungal drug and immunosuppressive agents etc.CONCLUSION:Hospital drug price level and percentages at each level tended to be more reasonable after many times policy price reduction.

Objective] Observation of treatment with traditional Chinese medicine mobile cupping combined with needle medicine rubella clinical curative effect, provide more favorable methods for clinical treatment of rubella means. [Methods] Use of stitch and cans together with professor Lu Xiaozuo Anshen Tongren acupuncture and Chinese medicine allergic to treatment of 128 cases rubella patients, 3 days for one time, needle time, 3 times a week, every time retaining needle 1 hour, 10 times for a period of treatment. Traditional Chinese medicine(TCM) on the basis of professor Lu allergy decoction, a month for a course of treatment. And make the clinical curative effect observation and analysis. [Result] Of 128 cases rubella patients, 56 cases cured, 47 cases were markedly effective, effective 14 cases, ineffective in 11 cases, total effective rate of up to 91.4%. [Conclusion] Rubella curative effect with traditional Chinese medicine mobile cupping method and needle acupuncture medicine is visible.

Objective To study the the effect of national free pre-pregnancy health check policy from the view of clinical labora-tory.Methods Couples who accepted pre-pregnancy check were recruited in the study.720 people were recruited.The data of pre-pregnancy health examination and related questionnaire were analyzed.Results In females,the IgG positive rates of rubella virus, cytomegalovirus and Toxoplasma were 80.34%,3.78% and 87.51%,respectively.IgM positive rates of Toxoplasma and cytomeg-alovirus were 0.73% and 1.24% respectively.17.98% females were found suffering from gynecological inflammation or ovarian cysts and other gynecological diseases.Syphilis test showed males positive rate was 0.83%,female positive rate was 0.59%.Ala-nine aminotransferase(ALT)test showed that male positive rate was 13.22%,female was 5.71%.For cognitive health examination surveys,92.33% males and 96.27% females had well known the importance of pre-pregnancy health examination.Conclusion Free pre-pregnancy health examination policy could provide a guarantee for the couple's family planning,and could reduce the incidence of malformation and neonatal congenital disease.

OBJECTIVE:The dissolution rates of baicalin in Gonglaoquhuo tablets were determined to provide a basis for evaluating and controlling the drug quality METHODS:The dissolution rates of Gonglaoquhuo tablets with different batch num_bers were determined by HPLC,using water as dissolution medium RESULTS:The T50 of Gonglaoquhuo tablets was 106min～220min and there were significant differences in dissolution parameters(T50,Td,m) between the tablets tested(P

It is of capital importance to establish a reliable experimental animal model of intracranial aneurysm which should be extremely similar to human intracranial aneurysm in histological and hemodynamic aspects.For recent years,the elastase-induced aneurysm model by means of endovascular balloon occlusion has been widely used abroad.This paper aims to review the preparation of animal mode,the advantages and disadvantages of such a model as well as the recent progress in its applications.

OBJECTIVE:To investigate the clinical effects of pidotimod in the bronchial asthma complicated with recurrent re-spiratory tract infection,and its effects on immunoglobulin and related indexes. METHODS:A total of 120 bronchial asthma pa-tients with recurrent respiratory tract infection selected from our hospital during Mar. 2011-May 2013 were divided into trial group and control group according to random number table,with 60 cases in each group. Control group received routine corticosteroid therapy,and trial group was additionally given Pidotimod oral solution 0.4 g,po,bid,for 14 d,on the basis of control group. Clinical indexes(the times of respiratory infection,the duration of fever,cough,wheezing attack and antibiotics use),serum in-dexes [β-defensin-1(hBD-1),immunoglobulin A(IgA),IgG,IgM,UREA,ALT],the results of pharynx test before and after treatment,and the occurrence of ADR were observed in 2 groups. RESULTS:Before treatment,there was no statistical signifi-cance in clinical indexes,serum indexes,the results of pharynx test between 2 groups(P>0.05). After treatment,clinical indexes of trial group were significantly lower or shorter then before treatment or control group,while serum levels of hBD-1,IgA and IgG were significantly higher than before treatment or control group,with statistical significance(P<0.05). There was no statistical sig-nificance in clinical indexes and serum indexes of control group,serum levels of IgM,UREA and ALT in trial group before and af-ter treatment(P>0.05). The types and number of pathogenic bacteria of respiratory tract infection were decreased significantly in 2 groups,and the trial group was significantly less then the control group,with statistical significance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Pidotimod shows good clinical effects on bronchial asthma complicated with recurrent re-spiratory tract infection,can improve immunity and reduce the types and number of pathogenic bacteria with good safety.

2,4,6-Trinitrotoluene (TNT) and its by-products dinitrotoluene (DNT) pose a significant threat to human health and other living organisms.However, the conventional analytical methods involved in bulky and expensive instruments are complicated and time-consuming, impeding quick and on-line determination.In this work, a facile yet effective strategy of utilizing UV-vis spectroscopy coupled with partial least squares (PLS) was proposed, through which TNT and two isomers of DNT (2,4-DNT and 2,6-DNT) in nature water could be rapidly and simultaneously determined without any pre-separation.Variable combination population analysis (VCPA) was utilized to select important feather variables and significantly improved the predictive performance of the PLS model.The calibration set contained 25 samples constructed by orthogonal array design (OAD).The predictive ability of the models was validated by an independent prediction set including 15 samples, achieving up to 0.99 of the determination coefficients (R2) for each of the analytes.The optimized models were successfully applied to determine the 3 ingredients in 8 environmental samples involving in tap, lake and two kinds of river water with the recovery values of great than 97%.Finally, the proposed method was further validated by high performance liquid chromatography method.UV-vis spectroscopy coupled with chemometrics may be used as simple and effective strategy with high potential in environmental monitoring.