1.The CorreIation of Serum BiIirubin and Hearing Loss of Neonate with Hypercholesterolemia
Sumin JIANG ; Shiiin LIU ; Li ZHANG ; Weiyi LIU ; Xuemei JIAO
Journal of Audiology and Speech Pathology 2015;(1):37-39
Objective To study the relationship between hearing loss and serum bilirubin of neonates with hypercholesterolemia .Methods The serum bilirubin level and auditory brainstem response thresholds of 70 hyper_cholesterolemia neonates of full term pregnancy and normal weight were tested ,and the correlations of serum biliru_bin ,and occurrence time of jaundice with hearing threshold were analyzed .ResuIts Among the 70 neonates ,there were 17 cases with normal ABR thresholds and 53 with abnormal ABR thresholds ,including 27 with middle and se_rious hearing loss .In the cases of serum bilirubin less than 342μmol/L ,the percentage of cases with middle and se_rious hearing loss and with occurrence time of jaundice less than 48 hours was higher than theses cases with occur_rence time of jaundice more than 48 hours (P<0 .05) .In the cases of serum bilirubin higher than 342μmol/L ,there was no obvious difference between the percentage of cases of middle and serious hearing loss with occurrence time of jaundice less than 48 hours and those with occurrence time of jaundice more than 48 hours .Among the 53 cases with abnormal hearing thresholds ,positive correlation was observed between serum bilirubin and ABR threshold (r=0 .041) ,whereas negative correlation was observed between occurrence time of jaundice and ABR threshold (r=-0 .291) .ConcIusion The higher probability of hearing loss for hypercholesterolemia neonates with earlier occur_rence of jaundice ,the more serious hearing loss with higher serum bilirubin .
2.Comparison between Astragalus membranaceus var. mongholicus and Hedysarum polybotrys based on ITS sequences and metabolomics.
Meili JIAO ; Zhenyu LI ; Fusheng ZHANG ; Xuemei QIN
Acta Pharmaceutica Sinica 2015;50(12):1625-31
Astragalus membranaceus var. mongholicus and Hedysarum polybotrys belong to different genera, but have similar drug efficacy in traditional Chinese medicine theory, and H. polybotrys was used as the legal A. membranaceus var. mongholicus previously. In this study, similarities and differences between them were analyzed via their ITS/ITS2 fragments information. The ITS (internal transcribed spacer) regions were amplified using polymerase chain reaction and then sequenced in two-way. The alignment lengths of ITS regions were 616 bp, in which 508 loci were consistent, and 103 loci were different, accounting for 82.47% and 16.72% of the total ITS nucleotides in length, respectively. As genotype determines phenotype, 1HNMR-based metabolomic approach was further used to reveal the chemical similarities and differences between them. Thirty-four metabolites were identified in the 1H NMR spectra, and twenty-seven metabolites were the common components. Amino acids, carbohydrates and other primary metabolites were similar, while a large difference existed in the flavonoids and astragalosides. This study suggests that A. membranaceus var. mongholicus and H. polybotrys show similarities and differences from molecular and chemical perspectives, which has laid a foundation for elucidating the effective material basis of drug with similar efficacy and resources utilization.
3.Fabricating facial prostheses using CAD/CAM and rapid prototyping technique.
Xuemei HUANG ; Ting JIAO ; Yanping LIN ; Wenqiang ZHANG ; Chengtao WANG ; Fuqiang ZHANG
Journal of Biomedical Engineering 2005;22(2):320-323
At present, the treatments of hemifacial microsomia such as the missing of ear and eye still rely on the skill of technician to make the wax model of contralateral apparatus of patient in China. In this paper, CAD/CAM and rapid prototyping (RP) techniques are integrated to successfully create a silastic prosthesis by using the patient's data of CT or laser scanning. The clinical results suggest that a high accuracy has been achieved in shape, size, and protrusion of the facial prostheses, which indicates that the application of RP techniques in conjunction with CAD/CAM is a suitable approach for fabricating facial prosthesis.
Biocompatible Materials
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chemistry
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Computer-Aided Design
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instrumentation
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Facial Asymmetry
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surgery
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Humans
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Image Processing, Computer-Assisted
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instrumentation
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methods
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Imaging, Three-Dimensional
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instrumentation
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methods
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Maxillofacial Prosthesis Implantation
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methods
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Numerical Analysis, Computer-Assisted
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Prostheses and Implants
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Prosthesis Design
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methods
4.Experience and analysis of excellent works of human parasitology in nation-al medical colleges and universities
Xiaodi YANG ; Qiang FANG ; Yang CHENG ; Xuelian CHANG ; Zhiyong TAO ; Yuanyuan WANG ; Xiaoli WANG ; Jiangyan LI ; Shiya HUANG ; Yumeng JIAO ; Xuemei WANG ; Hui XIA ; Xingzhi CHEN
Chinese Journal of Schistosomiasis Control 2017;29(3):369-371,376
Teaching competition is an effective way for college and university teachers to improve their teaching skills. Based on the teaching practice and experience in medical parasitology,this paper discusses several key issues in teaching competition including topics,teaching designs and teaching methods. It provides references for the teachers in department of parasitology of universities and colleges to improve the quality of classroom teaching.
5.MondoA Is Required for Normal Myogenesis and Regulation of the Skeletal Muscle Glycogen Content in Mice
Hui RAN ; Yao LU ; Qi ZHANG ; Qiuyue HU ; Junmei ZHAO ; Kai WANG ; Xuemei TONG ; Qing SU
Diabetes & Metabolism Journal 2021;45(3):439-451
Skeletal muscle is the largest tissue in the human body, and it plays a major role in exerting force and maintaining metabolism homeostasis. The role of muscle transcription factors in the regulation of metabolism is not fully understood. MondoA is a glucose-sensing transcription factor that is highly expressed in skeletal muscle. Previous studies suggest that MondoA can influence systemic metabolism homeostasis. However, the function of MondoA in the skeletal muscle remains unclear. We generated muscle-specific MondoA knockout (MAKO) mice and analyzed the skeletal muscle morphology and glycogen content. Along with skeletal muscle from MAKO mice, C2C12 myocytes transfected with small interfering RNA against MondoA were also used to investigate the role and potential mechanism of MondoA in the development and glycogen metabolism of skeletal muscle. MAKO caused muscle fiber atrophy, reduced the proportion of type II fibers compared to type I fibers, and increased the muscle glycogen level. MondoA knockdown inhibited myoblast proliferation, migration, and differentiation by inhibiting the phosphatase and tensin homolog (PTEN)/phosphoinositide 3-kinase (PI3K)/Akt pathway. Further mechanistic experiments revealed that the increased muscle glycogen in MAKO mice was caused by thioredoxin-interacting protein (TXNIP) downregulation, which led to upregulation of glucose transporter 4 (GLUT4), potentially increasing glucose uptake. MondoA appears to mediate mouse myofiber development, and MondoA decreases the muscle glycogen level. The findings indicate the potential function of MondoA in skeletal muscle, linking the glucose-related transcription factor to myogenesis and skeletal myofiber glycogen metabolism.
7.MondoA Is Required for Normal Myogenesis and Regulation of the Skeletal Muscle Glycogen Content in Mice
Hui RAN ; Yao LU ; Qi ZHANG ; Qiuyue HU ; Junmei ZHAO ; Kai WANG ; Xuemei TONG ; Qing SU
Diabetes & Metabolism Journal 2021;45(3):439-451
Skeletal muscle is the largest tissue in the human body, and it plays a major role in exerting force and maintaining metabolism homeostasis. The role of muscle transcription factors in the regulation of metabolism is not fully understood. MondoA is a glucose-sensing transcription factor that is highly expressed in skeletal muscle. Previous studies suggest that MondoA can influence systemic metabolism homeostasis. However, the function of MondoA in the skeletal muscle remains unclear. We generated muscle-specific MondoA knockout (MAKO) mice and analyzed the skeletal muscle morphology and glycogen content. Along with skeletal muscle from MAKO mice, C2C12 myocytes transfected with small interfering RNA against MondoA were also used to investigate the role and potential mechanism of MondoA in the development and glycogen metabolism of skeletal muscle. MAKO caused muscle fiber atrophy, reduced the proportion of type II fibers compared to type I fibers, and increased the muscle glycogen level. MondoA knockdown inhibited myoblast proliferation, migration, and differentiation by inhibiting the phosphatase and tensin homolog (PTEN)/phosphoinositide 3-kinase (PI3K)/Akt pathway. Further mechanistic experiments revealed that the increased muscle glycogen in MAKO mice was caused by thioredoxin-interacting protein (TXNIP) downregulation, which led to upregulation of glucose transporter 4 (GLUT4), potentially increasing glucose uptake. MondoA appears to mediate mouse myofiber development, and MondoA decreases the muscle glycogen level. The findings indicate the potential function of MondoA in skeletal muscle, linking the glucose-related transcription factor to myogenesis and skeletal myofiber glycogen metabolism.
9.Epidemiology of dental caries and its influences on jaw function in children
ZHANG Xuemei, MA Zheng, NIE Xiaohan, WEI Lin, WU Mixun, GUO Yibai, LYU Jiao
Chinese Journal of School Health 2023;44(1):123-126
Objective:
To understand and analyze the epidemiological characteristics of dental caries in children and its effects on jaw function, so as to prevent occurrence of abnormality of jaw function.
Methods:
In October 2020, 860 cases of children from 5 kindergartens and 3 primary schools in Beijing were selected by adopting the convenience sampling method. All of them received oral examination, and the questionnaire according to the fourth national oral health epidemiological survey standard, including demographic information, eating habits, oral hygiene habits, oral health care,etc. The epidemiological characteristics of dental caries in children and its effects on jaw function were analyzed.
Results:
A total of 190 children had dental caries, with a dental caries incidence rate of 22.09%. Dental caries in children s deciduous teeth or permanent teeth was related to age, residence, parents education level,whethe to eat before going to bed, whether eating sweet food, tooth brushing age, daily brushing frequency, whether parents have assisted tooth brushing, whether regular oral examination, whether parents have received oral health guidance( χ 2= 5.04 ,4.70,75.37,7.91,12.03,9.30,7.64,255.47,253.27,11.38, P <0.05). Compared with the noncaries group[(81.52±3.16, 80.54± 1.52,1.92±0.25,31.52±1.62,33.63±3.41,50.72±1.68)°], the sella nasion A point(SNA), sella nasion B point(SNB),A point nasion B point(ANB),frankfot horizontal mandibular plane angle(FH MP),skull nasion mandibular plane angle(SN MP),anteriors relationship palataplane mandibular(Ptm ANS) decreased in the dental caries group[(78.62±2.11,79.35±1.02,1.68±0.32,30.69±0.45,32.15±3.02,48.62±1.21)°](t=78.62,79.35,10.94, 30.69, 32.15, 48.62, P<0.05).
Conclusion
The prevalence of caries in children was high and jaw structure abnormalities and dysfunction. It is suggested that caries prevention and control work should be carried out early to strengthen the prevention and control of oral disease education work.
10.Potential targets and mechanisms of Guarana in the treatment of Alzheimer’s disease based on network pharmacology
Digital Chinese Medicine 2023;6(1):55-66
【Objective】 To dig the main active components and predict potential mechanisms of Guarana in the treatment of Alzheimer’s disease (AD) by network pharmacology method and molecular docking. 【Methods】 By digging into papers relating to this topic, chemical components in Guarana were obtained and used for drug-likeness analysis. Databases including HERB and Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) were used to obtain potential targets that the active components in Guarana might have effects on, and to find out diseases in association with the potential targets. Other databases such as GeneCards, a human gene database, and DisGeNET were used to identify the genes relating to AD, and a Wayne diagram was drawn to get the intersected targets in Guarana and AD. Subsequently, CytoScape software was adopted for the construction of a Guarana-intersected targets-AD map. After that, the intersected targets were uploaded to the Search Tool for the Retrieval of Interaction Gene/Proteins (STRING) database to acquire a protein-protein interaction (PPI) network diagram. Then, the key target proteins were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). In terms of molecular docking verification, AutoDock software was used to verify whether the crucial active compounds of Guarana’s components could bind to the key targets. 【Results】 A total of 140 potential targets for Guarana to treat AD were obtained. The results of PPI network analysis showed that interleukin 6 (IL-6), tumor necrosis factor (TNF), insulin (INS), mitogen-activated protein kinase 3 (MAPK3), transcription factor (JUN), cell tumor antigen p53 (TP53), caspase3 (CASP3), protein c-Fos (FOS), catalase (CAT), and Catenin beta-1 (CTNNB1) might be the key targets of Guarana in the treatment of AD. It was found by GO and KEGG analyses that the mechanism of Guarana in the treatment of AD might be the bindings between Guarana compounds and protease outside the cell membranes. The molecular docking results showed that the small molecules of various components in Guarana binding to target proteins such as TNF, IL-6, MAPK3, and FOS needed relatively less energy. 【Conclusion】 The treatment of AD with Guarana involves the participation of multiple targets, among which IL-6, TNF, and MAPK3 might be the key ones. These key targets might take effects through the biological process in their bindings to β-amyloid and involving signaling pathways in cancer. Hopefully, our research could offer some scientific foundations as well as references for in-depth studies on the treatment of AD with Guarana.