Objective To explore the clinical practice and patient experience of perioperative therapeutic communication in rectal cancer patients, in order to provide theoretical basis for the further clinical application of therapeutic communication. Methods To adopt phenomenology method of qualitative research, collecting data by the direct participation, observation, half structural formula thorough interview and other methods. To establish coding and refine theme. Results Four themes of the rectal cancer patients experience of perioperative therapeutic communication were generalized: emotional adaptation, actively coping with surgery, intraoperative cooperation, postoperative rehabilitation and self-care. Conclusions Application of therapeutic communication system can effectively interfere with patients cognitive behavior, improve communication ability of nurses and extended the nursing service connotation.

Objective To investigate the protective effects of melatonin and possible mechanisms on rats with alco-holic fatty liver (AFL). Methods All rats were randomly divided into 4 groups: normal group (n = 10), model group (n = 12) and melatonin groups (10 mg / kg, 20 mg / kg; n = 10, respectively). The model of rats’ alcoholic fatty liver was induced by intragastric influsion of ethanol for 8 weeks. The melatonin groups’ rats received melato-nin by intraperitoneal injection after intragastric infusion of ethanol. Histopathological changes were evaluated by hematoxylin and eosin staining. The expressions of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by immunohistochemical methods. The detection of aspartate aminotransferase (AST) levels and alanine transarninase (ALT) levels and the total bilirubin ( TBIL) levels in serum were provided by routine laboratory methods using an autoanalyzer. The levels of malondialdehyde ( MDA) and activities of glutathione peroxidase ( GPx) were measured by spectrophotometry. Results Compared with the normal group, the liver cells of the mod-el group showed obvious steatosis and significant swelling. However, less degree and less extensive of steatosis and swelling were observed in the melatonin groups. Compared with the normal group, the levels of ALT, AST and TBIL in serum and the levels of MDA in liver homogenates were significantly increased in the model group (P <0. 01), and the activities of GPx were distinctly decreased in the model group(P < 0. 01). But in the melatonin groups, the levels of ALT, AST and TBIL in serum and the levels of MDA in liver homogenates were decreased (P< 0. 01), and the activities of GPx were increased (P < 0. 01). Additionally, melatonin lessened the expression of TNF-α and IL-6 in liver obviously (P < 0. 01). Conclusion Melatonin may inhibit the development of alcoholic fatty liver and its possible mechanism is the ability to resist oxidative stress and lessen the expression of TNF-α and IL-6 and other relevant factors in liver.

Objective To explore the diagnostic value of plasma fetal DNA level in preeclampsia.Methods Thirty cases of pregnant women with preeclampsia(at 33 weeks and 3 days) and 30 cases of normal pregnant women(at 34 weeks and 3 days) were selected.All the pregnant women carried a male fetus by B-ultrasound,and were sampled at gestational 20 weeks,third trimester and at 1 hour,3 hours,6 hours after delivery.SRY levels in maternal blood were quantitated by polymerase chain reaction(QF-PCR).The endotheliotoxin(ET) level was measured with RIA.Results (1) Mean fetal DNA level of patients with preeclampsia at 20 weeks of gestation was(316?61)copy/ml.They were(266?79)copy/ml,(396?91)copy/ml,(165?43) copy/ml for light and severe preeclampsia women and normal pregnant women,respectively.Maternal blood fetal DNA levels in pregnant women with preeclampsia at 20-weeks of gestation were significantly higher than those normal pregnant women(P

Objective To investigate the effects of Jiawei Wuzi Yanzong Pill and its components on central cholinergic system in the model mice with learning and memory disorders induced by scopolamine. Methods Mice were treated by Jiawei Wuzi Yanzong Pill and its components for 15 days and induced by ip scopolamine. After the experiment in Morris water maze, the content of acetylcholine and the activity of acetylcholinesterase from brains of mice were determined by colorimetry. Results Significant differences were observed between model group and naked group that the content of acetylcholine and the activity of acetylcholinesterase from brains of model mice were decreased (P 0.05). Conclusion Jiawei Wuzi Yanzong Pill and its components could improve the function of central cholinergic system by increasing the content of acetylcholine and the activity of acetylcholinesterase and possesses a certain preventive effects on mice with learning and memory disorders induced by scopolamine.

Objective To know the influence of psychological intervention on delivery results of par-turient women, and then reference to certain effective psychological nursing cares for clinical field. Methods Divided 226 partreient women of spontaneous labor into the experimental group and the control group randomly, there were 113 eases in each group. Traditional routine nursing cares was used in the control group, psychological intervention was used in the experimental group in addition. Compared the condi-tion of birth process and the incidence rate of labor-related complications between the two groups. Re-sults There were significant differences between the two groups about all the indexes which had indicated the delivery. Conclusions Correct psychoanalysis and proper nursing intervention and effective release the pains for parturient women, and then decrease the incidence rate of medical negligence.

Objective To understand the dietary intake of pregnant women, and try to give a reasonable sug-gestion to promote fetal development. Methods 400 pregnant women in Fushan city were enrolled in this study and their dietary intake were investigated, then the software of Analysis of pregnancy nutrition guidance system was used to make nutrition analysis. Results The ratio of the energy in each pregnancy provided by dietary protein, carbohy-drate and fat were relatively appropriate. But the intake of dietary calcium, ferrum, zinc, vitamin B1 and vitamin B2 in early pregnancy were very low, the intake were respectively 764.51 mg, 26.94 mg, 15.71 mg, 1.12 g, 1.13 g. Each pregnancy the ratio of high-quallty protein in total protein intake were more than 50%, an average protein RNI% was 48.50%. Conclusion The dietary style of pregnant women is relatively reasonable. But they should strengthened nutritional guide to intake more calciuro,ferrum, zinc,vitamin B1 and vitamin B2.

ObjectiveTo investigate and evaluate the depression in patients with type 2 diabetes.Methods62 patients with type 2 diabetes (30 having complications, 32 not having complications) and 20 normal controls were assessed by Hamilton Depression Rating Scale (HRSD) of Peking University PES Mental State Application System.ResultsThe HRSD total scores of diabetics were higher than that of normal controls, and scores of diabetics with complications were higher than that without complications (P<0.05). The scores of anxiety/somatization and cognitive disturbance of diabetics were all higher than that of normal control (P<0.05).The scores of sluggish, dyssomnia of diabetics with complications were higher than that of normal controls (P<0.05), but there was no difference between diabetics without complications and normal controls (P>0.05). The scores of changing at day and night, weight, and the despair feel of diabetics were not obviously different with that of normal controls (P>0.05).ConclusionThe diabetics are often companioned with depression, and more severe when having complications.

Purpose To observe the action time of parthenolide (PTN) as selective nuclear factor-κB(NF-κB) inhibitor in heart of rats.Methods Forty SD rats were randomly divided into control(CON) group,PTN group,dimethyl sulfoxide(DMSO) group,lipopolysaccharide(LPS) group and PTN+LPS group.The first day:PTN group,DMSO group and PTN+LPS group rats were respectively injected interapertoneally PTN(500 μg/kg) and DMSO; other groups rats were injected interapertoneally equivalent saline.The second day(in equivalent to 24 h after injecting PTN):in LPS group and PTN+LPS group LPS(12.5 mg/kg) was administered subcutaneously; other group rats were injected subcutaneously equivalent saline.Every heart was obtained in equivalentce to 1 h after injecting LPS to measure the expression of NF-κB p50 in nucleus,inhibitory kappaB(IκBα) and phosphorylation inhibitory kappaB(p-IκBα) in cytoplasm of cardiac myocytes through Western blotting.Results Compared with CON group,the expression of NF-κB p50,IκBα and p-IκBα had no difference in PTN and DMSO group.And in LPS group and PTN + LPS group,the expression of NF-κB p50 up-regulated significantly(P＜0.05),IκBα down-regulated(P＜0.05) and p-IκBα up-regulated significantly(P＜0.05).Conclusion LPS can activate NF-κB in 1 h and PTN in rats after 24 h has few effect.

Objective:To analyze therapeutic effect of rosuvastatin on inflammatory factor levels ,insulin resistance (IR) and vascular endothelial function in patients with hypertension complicated dyslipidemia .Methods:A total of 136 outpatients with hypertension complicated dyslipidemia were selected .According to random number table ,they were divided and equally into routine treatment group (received routine therapy ) and rosuvastatin group (received rosuvastatin based on routine treatment ) .Serum levels of C reactive protein (CRP) ,interleukin‐6 (IL‐6) and IL‐8 , IR and vascular endothelial function were compared between two groups before and after treatment .Results:Com‐pared with before treatment ,there were significant reductions in serum levels of CRP ,IL‐6 and IL‐8 ,homeostasis model‐insulin resistance index (HOMA‐IR) and number of endothelial microparticles (EMPs) ,significant rise in in‐sulin sensitivity index (ISI) and flow‐mediated dilation of brachial artery (FMD) after treatment , P< 0.05 all . Compared with routine treatment group ,there were significant reductions in serum levels of CRP [ (67.27 ± 7.51) mg/L vs .(37.11 ± 6.32) mg/L] ,IL‐6 [ (87.58 ± 7.21)μg/L vs .(60.17 ± 5.45)μg/L] and IL‐8 [ (121.31 ± 8.57)μg/L vs .(84.44 ± 5.21)μg/L] ,HOMA‐IR [ (3.08 ± 0.51) vs .(2.31 ± 0.47)] and number of EMPs [ (852.18 ± 115.37) /μl vs .(573.29 ± 72.18)/μl] ,and significant rise in ISI [(-4.39 ± 0.61) vs .(-3.42 ± 0.53)] and FMD [ (4.35 ± 0.52)% vs .(5.82 ± 0.69)% ] in rosuvastatin group after treatment ,P<0.05 all .Conclusion:Rosuvasta‐tin could reduce inflammatory factor levels ,relieve insulin resistance and improve vascular endothelial function in patients with hypertension complicated dyslipidemia .

Objective To explore the effects of human menopausal gonadotropopin(HMG) supplementation on the outcome of women underwent in vitro fertilization-embryo transfer(IVF-ET) .Methods The data of 406 IVF-ET cycles in Reproductive Medi-cine Center of the 105th Hospital of PLA were analyzed retrospectively .All cases underwent long down regulation protocol with gonadotropin releasing hormone agonist(GnRH-a) in the mid-luteal phase and controlled ovarian stimulation(COS) was carried out with follicle stimulation hormone(r-FSH) on the days 3 -5 of the menstrual cycle .Then 75 -150 U HMG was administrated in group A(257 cycles) when a dominant follicle reached a diameter of 14 mm ,while the remaining cases(149 cycles) underwent HCG still with r-FSH were served as group B .Based on the LH levels on the day of HMG administration ,the cases in group A were sub-divided into :group A1(99 cycles) ,LH<1 U/L ;group A2(96 cycles) ,1 U/L≤LH≤2 U/L ,and group A3(62 cycles) ,LH>2 U/L .Clinical outcomes of all groups were analyzed and compared .Results The durations and doses of gonadotropin(Gn) ,the rates of fertilization and pregnancy were higher and the abortion rate was lower in group A than that in group B (P<0 .05) .There were no significant difference in serum LH concentrations on the days of HMG and HCG administration ,oocytes retrieved ,the rates of cleavage and embryo implantation between group A and group B(P>0 .05) .There was significant difference in serum LH levels on the day of HMG supplementation among group A1 ,A2 and A3(P<0 .05) and the doses of HMG supplemented reduced gradually from group A1 to group A3(P<0 .05) .The duration of Gn was significantly lower and the fertilization rate was significantly higher in group A3 compared with group A1 and A2(P<0 .05) .The pregnancy rate in group A2 and A3 was higher than that in group A1 ,which showed significant difference between group A2 and A1(P<0 .05) .Meanwhile ,there were no significant difference in doses of r-FSH ,serum LH concentrations on the day of HCG administration ,oocytes retrieved ,the rates of cleavage ,implantation and abortion among the three groups(P>0 .05) .Conclusion HMG supplementation in the middle and late follicle phases in stand-ard long down-regulation protocol during IVF could obtain higher pregnancy rate and lower abortion rate ,especially when their ser-um LH level was between 1 U/L and 2 U/L without obvious increase of LH .