Objective To evaluate the clinical efficacy of cefotaxime combined with gamma globulin on neonatal septicemia. Methods The subjects of this study were selected from 88 cases of neonatal septicemia admitted in Jiangyou No.903 hospital from April 2014 to April 2017. They were randomly divided into two groups, each with 44 cases. The control group was given symptomatic treatment and cefotaxime, while the observation group symptomatic treatment, cefotaxime and gamma globulin as well. After 7days of treatment, the overall effective rates, case fatality rates, clinical symptoms (body temperature, resistance to milk, neurological symptoms), time for improvement and hospital stay were compared between the two groups. Results The overall effective rate of the observation group was 95.45％, much higher than 72.73％ of the control group (P<0.01). The observation group had much lower case fatality rate than the control group did (2.27％ vs 18.19％) (P<0.05). The time the observation group took for improvement in the condition of body temperature, resistance to milk, neurological symptoms, and hospital stay was (3.25±1.07) days, (4.93±1.96) days, (5.92±1.58) days, and (6.80±1.94) days respectively, all significantly shorter than the time the control group took (P<0.01). Conclusion The combination of cefotaxime and gamma globulin is effective in the treatment of neonatal septicemia. It can significantly reduce case fatality rate and shorten the time for clinical symptoms and hospital stay.

Objective To observe the impact of hyperbaric oxygen (HBO) therapy on blood pressure and heart rate in adults with spinal cord injury (SCI). Methods 48 adult SCI patients who accepted HBO therapy from March to November, 2014, were observed. Other 48 non-SCI patients matched sexes and ages were as the controls. Their blood pressure and heart rate were measured before chambering, before compression, HBO for 30 minutes, before decompression and at the end of decompression. The patients were divided into high SCI group and low SCI group based on the injury levels above or below T4. Results The blood pressure was lower in the high SCI patients than their controls (t>6.337, P<0.001), and increased as taking in the oxygen. There was no significant difference between the high SCI patients than their controls as they took in for 30 minutes and before the decompression (P>0.05). No difference was found between both groups in heart rate (P>0.05). For the low SCI patients, neither the blood pressure nor the heart rate was different from their controls (P>0.05). Conclusion The blood pressure may increase during HBO intaking in the high SCI patients, which need be paid attention to.

Objective To study high-frequency ultrasonic features of pigmented villonodular synovitis (PVNS).Methods High-frequency ultrasonic findings in 17 cases with PVNS were analyzed and a comparison between surgical and pathological results was made.Results Of 17 cases,10 cases were diffuse type,7 cases were local type.The main high-frequency ultrasonic signs were as follows: the diffused type synovial PVNS demonstrated as hypoecho,local synovial proliferation was revealed as nodulated and heterogeneous echo,hydrarthrosis of the knee joint was present in 10 cases,the involvement of meniscus occurred in 4 cases,intra-articular cartilage and cortex erosion were showed in 5 cases.Local type PVNS was mainly demonstrated as the nodulated and hypoechoic proliferation of synovium,hydrarthrosis of the joint and cartilage erosion were not found.Conclusions High-frequency ultrasound is a valuable imaging method for diagnosing PVNS in preoperative routine screening.

Objective The amount of neuroglobin(Ngb) in cerebral cortex after different post-traumatic time intervals were studied in order to find out the value of Ngb in estimating the survival time after trauma.Methods Moderate brain contusions in rats were made using free-falling-body impact device,The area and gray values of Ngb immunoreactive products(Ngb-IR)in the core of injury in cerebral cortex and penumbra and the contra lateral cerebral cortex at different time points after trauma were studied by immunohistochemical method and image analysis.Results The expression of Ngb in the core of injured cerebral cortex declined quickly and reached the nadir by 24 hours and remained at low level for long time.On the contrary,it rose quickly and reached the peak value at 12 hours in the penumbra surrounding the core of injury,then declined gradually and reached normal level at 8d to 16d.Image analysis revealed that the expression of Ngb-IR in the penumbra of injured cortex were stronger than that in the opposite side.Conclusion The amount of Ngb in cerebral cortex at the core and penumbra of injury changed regularly according to different time intervals after trauma.Ngb may play an important role in neuroprotection during acute phase of injury.

Self-assembled gold nanoparticles ( AuNPs) coating was fabricated using an etched stainless steel wire as a support on which AuNPs were first deposited, then after modified with mercaptan, another layer of AuNPs was self-assembled. The AuNPs modified stainless steel wire was used in solid phase microextraction (SPME) coupled with high performance liquid chromatography (HPLC) for the determination of ultraviolet ( UV) filters in environmental water. The best extraction efficiencies were achieved within 30 min at 55℃ and at pH 7 with stirring rate of 800 r/min. Under the optimized conditions, the established AuNPs-SPME-HPLC method for the determination of UV filters benzophnone-3 ( BP-3 ) , 2-ethylhexyl-4-( N, N-dimethylamino ) benzoate ( OD-PABA) , 2-ethylhexyl-4-trimethoxycinnamate ( EHMC) and 2-ethylhexyl salicylate ( EHS) was linear in the range of 0. 004-200 μg/L. The limits of detection of the method were 0. 43-570 ng/L ( S/N=3 ) . The relative standard deviation ( RSD ) of AuNPs-SPME-HPLC was 1 . 9%-4 . 2% ( n=5 ) for spiked water samples of 20 μg/L each UV filter. In the case of real water samples analyses, the recoveries of spiked UV filters were 77 . 9%-108% with RSDs of 3 . 1%-8 . 0% ( n=5 ) .

Gui-Ling-Ji (GLJ), a classical prescription of traditional Chinese medicine (TCM), is the only existing compound of refining agent after 400-year testing. It is also one of 4 kinds of TCM secret varieties of the first batch after liberation. However, due to the lack of in-depth modern research, the magical effect of GLJ has not been fully understood and the clinical application has also been influenced. This article analyzed the situation and problems of secret varieties and GLJ. Experiences and strategies of the successful redevelopment of secret varieties were re-ferred to. Then, this paper proposed modern research thoughts of GLJ in order to realize the actively protection and the second development of the prescription.

AIM:To set up a glutamate-induced cell damage model in cultured hippocampal neurons, and to determine whether glutamate-induced neuronal apoptosis changes and whether this process is mediated by mitochondrial signal transduction pathways involving the release of cytochrome C. METHODS: Hippocampal neurons, isolated and cultured from new born Wistar rats, were exposed to various concentrations of glutamate. Extent of cell death was assessed by measuring the release of lactate dehydrogenase (LDH) in the culture media. Based on these data, an appropriate concentration of glutamate was selected, and all subsequent experiments were carried out under the concentration. Kinetics of glutamate-induced both apoptotic and necrotic cell death after exposure to glutamate for various times(3-24 h) were determined by flow cytometry and LDH release. The caspase-3 protein levels and cytochrome C release from mitochondria into cytosol in hippocampal neurons were determined by Western blotting. RESULTS: Glutamate treatment induced hippocampal neurons death in dose-dependent and time-dependent manners. A significant increase in LDH release (18.4%) was induced in the cells treated with 50 ?mol/L glutamate, compared to control untreated cells(P

Background and purpose:Stanniocalcin 1 (STC1) has been reported to be up-regulated in various cancer tissues, and related to malignancy degree of cancer. However, the molecular mechanism of STC1 in lung cancer cells is still not clear. This experiment aimed to investigate the effects of STC1 on cell cycle and apoptosis of lung cancer A549 cells.Methods:A549 cells were transfected with validated siRNA for STC1 A549-STC1-siRNA and a negative control vector RNA A549-Vector. The gene and protein expression of cell cycle-related genes, including CyclinA, CyclinB1, CyclinD1, CyclinE, CDK2 and CDK4, as well as apoptosis-inhibiting genes Bcl-2, Bcl-xl and apoptosis-inducing genes Caspase-3, Bax, Bak and Bid, were detected by real-time lfuorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot. The cell cycle distribution was determined with lfow cytometry. Terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) was used to detect cell apoptosis.Results:After transfection with STC1-siRNA, the gene and protein expression of CyclinA, CyclinB1, CyclinD1, CyclinE, CDK2 and CDK4 decreased signiifcantly in A549 cells (P<0.05). The proportion of cells in G0/G1 phase signiifcantly increased,
whereas the proportion of cells in S phase and G2/M phase decreased (P<0.05). The cell cycle was blocked at G0/G1 phase. Furthermore, compared with that in A549-Vector, the gene and protein expression of Bcl-2 and Bcl-xl in A549-STC1-siRNA was reduced signiifcantly (P<0.05), while the expression of apoptosis-inducing genes Caspase-3, Bax, Bak and Bid increased obviously (P<0.05). In addition, the percentage of apoptotic cells significantly increased in A549-STC1-siRNA compared with that in A549-Vector detected by TUNEL method.Conclusion:Down-regulation of STC1 by RNAi can block the cell cycle of A549 cells, inhibit cell proliferation, and promote cell apoptosis.

Objective To analyze the HLA - DRB1 gene polymorphism and the hereditary association with unexplained recurrent spontaneous abortions(URSA)in a population of Tujia and Han nationality from west part of Hunan province in China. Methods The alleles at the HLA-DRB1 typing were analyzed by polymerase chain reac-tion-sequence specific primers (PCR-SSP) in 76 URSA Tujia population and 68 URSA Han population and 82 healthy Tujia population and 75 healthy Han population. Results ①DRB1*04 frequency of URSA in Tujia and Han population was strikingly higher than that of the healthy Tujia and Han population(RR>1,Pc <0.01),but the DRB1*12 frequency was strikingly lower than that of the healthy Tujia and Han population respectively(RR<1,Pc <0.01). ②DRB1*07 frequency of URSA in Tujia population was strikingly higher than that of the URSA in Han population (18.08% : 5.28%, Pc<0.01). Conclusion HLA-DRB1*04 maybe predisposing genes of URSA in Tujia and Han population,and HLA-DRB1*12 maybe associated with an antagonist effect in the pathogenesis of UR-SA in this population.

Objective To study the effect of different grades of gastric mucosa epithelial neoplasia tissues of Helicobacter pylori (Hp ) infection and the relationship between chemokine CXCL12 and its receptor CXCR4 expression and significance. Methods Based on a total of 138 cases of endoscopic specimens, there were normal gastric mucosa (NGM) in 32 cases, low grade intraepithelial neoplasia (LGIEN) in 35 cases, high grade intraepithelial neoplasia (HGIEN) in 36 cases, and gastric adenocarcinoma (GCA) in 35 cases. Hp infection was determined by 13C breath test and serum Hp antibodies,the double positive for Hp infection. CXCL12 and its receptor CXCR4 were detected by immunohistochemical method. Results The positive rates of CXCL12 and CXCR4 in LGIEN,HGIEN and GCA were higher than those in NGM:71.43%(25/35), 86.11%(31/36), 91.43%(32/35) vs. 25.00%(8/32);71.43%(25/35), 86.11%(31/36), 91.43%(32/35)vs. 28.12%(9/32), P<0.05;the positive rates of CXCL12 and CXCR4 in HGIEN and GCA were higher than those in LGIEN (P<0.05);the positive rates of CXCL12 and CXCR4 in HGIEN and GCA had no significant difference (P>0.05). The positive expression of CXCL12 and CXCR4 had no correlation with Hp infection (P>0.05). Conclusions The positive rates of CXCL12 and CXCR4 in LGIEN,HGIEN and GCA are higher than those in NGM.The positive rates of CXCL12 and CXCR4 in HGIEN and GCA are higher than those in LGIEN. The positive rates of CXCL12 and CXCR4 in HGIEN and GCA are similar. The positive expression of CXCL12 and CXCR4 have no correlation with Hp infection, it suggests that Hp infection may not play oncogenic role by CXCL12 and CXCR4 receptor expression.