BACKGROUND:Titanium zirconium niobium tin al oy (Ti-Zr-NB-Sn al oy) has good tissue structure, mechanical properties, corrosion resistance and biocompatibility, which is an ideal dental material. OBJECTIVE:To explore the efficacy and safety of Ti-Zr-NB-Sn al oy in dental restoration. METHODS:A retrospective analysis was performed on clinical data of 87 cases of dental defects, including 43 males and 44 females, aged 22-41 years. Of the 87 cases, 43 cases were repaired with Ti-Zr-NB-Sn al oy as observation group, and the other 44 cases were treated with nichrome as control group. After 36 months of fol ow-up, the therapeutic efficacy, dental restoration conditions, dental prosthesis conditions and patient satisfaction were observed and compared. RESULTS AND CONCLUSION:Al the 87 patients were fol owed up for 36 months. After the fol ow-up, the total effective rate and patient satisfaction were higher in the observation group than the control group (P<0.05). In the control group, there were four cases of swol en gums, four cases of bleeding gums, four cases of al ergies, three cases of gum gray line, and six cases of mucosal pain;in the observation group, there was only one case of swol en gums, and the dental restoration was better in the observation group than the control group (P<0.05). For the dental prosthesis, there were 10 cases of dental stent damage and 20 cases of dental stent loosening in the control group;and only one case of dental stent damage and one case of dental stent loosening in the observation, and the observation group was superior to the control group in the dental prosthesis (P<0.05). Experimental findings suggest that Ti-Zr-NB-Sn al oy for repair of dental defects has good biocompatibility and security.

NM2 3genewasconsideredasatumormetastasissuppressor,itisalsoinvolvedintumor pathogenesis ,therearemoredetailedstudiesinbreastcarcinoma .Thisreviewfocusesontherelationshipbe tweenNM2 3geneandthemetastasisinbreastcarcinoma .

ObjectiveTo evaluate the effect of the comprehensive surgical management of primary venous reflux disease in lower extremity. MethodsComprehensive surgical management was performed on 108 patients( 126 limbs) with primary venous reflux disease between February, 1996 and Sept, 2000. All limbs were performed with superficial venous operation. External valvuloplasty of femoral vein valve was given to 83 cases(97 limbs) simultaneously, and to 22 cases (26 limbs) with concomitant subfascial endoscopic perforator surgery(SEPS). 3 cases (3 limbs) were operated with concomitant SEPS only. ResultsVenous claudication,swelling, and aching disappeared in 80.2% of limbs( 101/126) and apparent improvement in 19.8 % of limbs(25/126). All varicose veins were gone. Ulcer healing pre sented in 80 % of the limbs(8/10) with ulcers and ulcer decreasing in 20% of the limbs(2/10) in 3～6 months after superficial and deep venous operations. Ulcer healing presented in 23 cases(26 limbs) with ulcers and ulcer decreasing in remaining 2 limbs in 14 to 32 days after additional SEPS besides superficial and or deep venous operations. Deep venous valve sufficiency was demonstrated in 89.7% (113/126) of the limbs by Color Duplex. ConclusionIn the patients with severe primary venous reflux disease,comprehensive surgical management with concomitant superficial, deep and perforating veins might greatly improve the clinical effect.

Objective To evaluate the clinical efficacy and safety of bicyelol tablets in treatment of hepatitis B patients infected with YMDD mutation of HBV. Methods Sixty-eight chronic hepatitis patients infected with HBV YMDD mutants and 100 patients with non-mutants HBV were enrolled in the study. All patients received bicyclol tablets orally 150 mg/d, t. i. d, for 24 weeks. Clinical symptoms, signs and adverse effects were observed, and the blood routine, liver function tests, serum HBV markers and HBV DNA loads were examined at 12th and 24th week of the study, Results After treatment for 24 weeks, the normalization rates of ALT and AST in mutant group were 79.4% ( 54/68 ) and 70. 6% (48/68) ; 11 ( 16. 2% ) patients were markedly effective and 14 (20. 6% ) were effective. Clearance rates of HBeAg and HBV DNA were 27.9% ( 17/61 ) and 17.6% ( 12/68 ), while the seroconversion rate of HBeAg was 14. 7% (9/61). The differences of the above indexes were not statistically significant between mutant group and non-mutant group. Conclusion Bicyciol can both protect liver functions and inhibit virus replication in patients infected with HBV YMDD mutants.

BACKGROUND:Studies demonstrated that,the probability of middle and distal end of femur bone fatigue or fracture reaches maximum when the moving loads increase or under an impact load.OBJECTIVE:To analyze the stress-strain distribution,principle stress vectors and displacements of femur during gait.METHODS:The contour curves of femur and muscles attached to femur were extracted slice by slice based on the serial CT data from a healthy male volunteer,which were used to build musculoskeletal system models.And the straight-line muscle models of the lower extremities were constructed to evaluate muscles forces and joints forces by using a multi-objective optimization method based on gait analysis.The finite element models of femur during gait were built by taking muscle forces and joint forces as boundary conditions in ANSYS 10.0 to obtain the stress-strain distribution and displacements.RESULTS AND CONCLUSION:The maximal displacement occurred on the femur head,and the maximal stress occurred in the middle and distal end of femur during the whole gait.There exists bigger tensile stress in the location of the femur neck and middle and distal end of corpus ossis femoris.The result validates the fact that femur fracture often happens at the middle and below of femur stem from the viewpoint of biomechanics.

Objective　In order to explore the prevalence of hypophosphatemia in postoperative patients of general surgery,as well as the time and dosage of phosphate replacement and its effects.Methods　To serum phosphate levels of 45 patients with phosphate replacement begun on the second postoperative day and 45 without replacement were monitored at the preoperative day,and the first,third,fifth and seventh postoperative day.Results　The incidence of hypophosphatemia was 91.1%(41/45) in cases without phosphate replacement.While it was 57.8%(26/45) in those with replacement.The serum phosphate concentrations in cases with phosphate replacement were higher than those without replacement on the third(P<0.001),fifth(P<0.05) and seventh(P<0.01) postoperative day.The Apache Ⅱ scores in cases with phosphate replacement decreased on the third postoperative days compared with those on the first day(P<0.001).Conclusion　The postoperative replacement of phosplates can avert the occurrence of hypophosphatemia and improve the Apache Ⅱ score.

Objective To study the influence of the modification of the special neutralizing epitopes of the HIV-1 envelope (Env) on its assembly of functional pseudovirus and neutralizing activity. Methods Site-directed mutations were performed using cycling mutagenesis and selection of mutants with Dpn I . With this method, the 2G12 and 2F5 neutralizing epitopes were integrated into Env of subtype BC which was without the two epitopes, then the capability of forming pseudovirus and the neutralizing activity against 2G12 and 2F5 were compared with pre-modified Env. Results The special Env neutralizing epitopes of five HIV pseudovirus (BC02, BC03, BC04, BC05 and BC12) were modified. Among the five pseudovirus, BC04 and BC12 pseudovirus can't be formed after the 2G12 epitope was modified, whilst the BC02, BC03 and BC05 pseudovirus can be formed after the 2G12 and 2F5 epitopes were added, and there was no variation of the pseudovirus titer; On the aspect of neutralizing activity, BG03 pseudovirus against 2G12 and 2F5 was enhanced, BC02 and BC05 pseudovirus against 2F5 was enhanced while which against 2G12 was not changed. Conclusion The modification of 2G12 epitope influences the forming of pseudovirus and the addition of neutralizing epitopes can enhance the neutralizing activity of pseudovirus, which offers new approach for the optimization of HIV immunogen.

Four hundred eighty patients with carbon monoxide poisoning were randomly assigned to receive hyperbaric oxygen alone or hyperbaric oxygen plus Nimodipine.Treatment outcomes and the incidence of delayed encephalopathy after acute carbon monoxide poisoning(DEACMP)were observed.We found that the incidence of DEACMP was 14.0％(67/480)in all cases,19.2％(46/67)in hyperbaric oxygen group,and 8.8％(21/67)in hyperbaric oxygen plus Nimodipine group(hyperbaric oxygen group vs hyperbaric oxygen plus Nimodipine group,P＜0.05).These results suggested that hyperbaric oxygen combined with Nimodipine could prevent the development of DEACMP.

Objective:To investigate the mechanism of survivin, p53 and Ki-67 on Hep-2 human laryngeal cancer endothelial cell proliferation and invasion.Methods:Laryngeal squamous cell carcinoma and paracancerous normal tissues were collected, total RNA was extracted from tissues,survivin,p53and Ki-67gene mRNA expression levels in laryngeal cancer and the adjacent tissues were detected by Real-time PCR. Human laryngeal cancer Hep-2 epithelial cells were selected,survivin gene was overexpressed, and cell proliferation was detected by MTT.p53 andKi-67gene expression changes in overexpressedsurvivin gene were detected by Western blot. Changes in Hep-2 cell invasive ability were studied whensurvivin was overexpressed as detected by Transwell invasion assay.Results: In the adjacent tissues, survivin,p53andKi-67 gene relative expression levels were 1.72 ± 0.9, 13.7 ± 5.7 and 5.7 ± 1.3, respectively; while in cancer tissues, gene relative expression levels were 53.7 ± 8.3, 66.7 ± 5.2 and 61.0 ± 3.1, respectively, which was significantly increased. As detected by MTT, relative cell survival rate within 12 h ofsurvivinoverexpression were: load control group, (88.5±1.6)%; overexpressed group, (90.3±1.9)%. Transwell invasion assay results indicated that overexpressedsurvivincould significantly increase the relative survival rate of cells. Conclusions:Expressions ofp53,Ki67 and survivin are increased in cancer; and there is a positive correlation betweensurvivin, p53andKi67 expressions in laryngeal carcinoma.

Objective To evaluate the expression and clinical significance of plasma circulating microRNA-23b in patients with myasthenia gravis (MG).Methods Plasma samples from 32 MG patients and 32 healthy people were collected in the Second Affiliated Hospital of Harbin Medical University from October 2015 to August 2016. Expression of microRNA-23b was detected by quantitative reverse transcription polymerase chain reaction .The relationship among microRNA-23b expression level, clinical features and quantitative myasthenia gravis score ( QMG ) was analyzed. Results The expression of circulating microRNA-23b in plasma was significantly higher in MG patients (4.587 (3.654, 5.423)) compared with control group (2.889 (1.968, 4.027), Z=-4.169, P<0.01), meanwhile it was positively related to QMG (r=0.661,P<0.01).But the expression of circulating microRNA-23b had no statistically significant difference in MG subgroups .Conclusion Plasma circulating microRNA-23b is highly expressed in MG and has positive correlation with MG severity .