Objective To study the influence on T-lymphocyte subsets in herpes zoster with adjuvant treatment of compound glycyrrhizin.Methods 93 patients with herpes zoster were randomly divided into two groups:control group(46 cases,treated with antiviral drugs) and 1 treatment group(47 cases,treated with compound glycyrrhizin and antiviral drugs).The expression of T-lymphocyte subsets on the T cell surface in patients and control group were determined by flow cytometry.Results The expression of CD3,CD4,CD8,CD4/CD8 ratio was significantly different in the treatment group and control group.Conclusion Compound glycyrrhizic adjuvant therapy could not only shorten check blister,acetanilide,scar tissue and recovery time,reduce the sequela of neuralgia,but also affect the expression state of T lymphocyte subsets with herpes zoster,improve the cellular immune function with herpes zoster.

Objective To investigate the roles of serum visfatin and VE-cadherin in the pathogenesis of psoriasis vulgaris and their clinical significance. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of visfatin and VE-cadherin in 78 patients with psoriasis vulgaris before and after treatment and in 30 normal human controls. The relationship between the two parameters and disease activity was evaluated. Results The serum levels of visfatin and VE-cadherin were significantly higher in untreated patients with psoriasis than in the normal controls (I = 10.53, 10.16, both P< 0.01), and in patients with active psoriasis than in those with stable psoriasis (t = 12.47, 13.11, both P< 0.01). The treatment caused a marked decrease in the serum levels of visfatin and VE-cadherin in patients. The serum level of visfatin was positively associated with the level of VE-cadherin and PASI scores (r = 0.58, 0.64, P < 0.01) in patients with psoriasis. Conclusion Visfatin and VE-cadherin may be used as parameters for evaluating disease activity of psoriasis vulgaris.

OBJECTIVE: To investigate the epidemic features of childhood allergic rhinitis (AR) and correlative risk factors in Yinchuan. METHOD: A questionnaire survey on AR was conducted among 800 children in eight communities, aged from 5 to 14 years old, by random cluster sampling, from March 2012 to March 2013 in Yinchuan. Suspects of AR, as identified by the survey, were recommended to specialist examinations for accurate diagnosis. The results were statistically analyzed. RESULT: Seven hundred and sixteen questionnaires were returned and 662 questionnaires were available. The prevalence rate of AR in children aged from 5 to 14 is 14.65% in Yinchuan. Seventy-nine point thirty-eight percent children suffered from intermittent AR, while 20.61% children suffered from persistent AR. The difference of prevalence between female (13.57%) and male (15.44%) was insignificant statistically (Χ2=0.454, P>0.05). The difference of prevalence between the Han (15.72%) and the Hui (11.35%) insignificant statistically (Χ2=2.055, P>0.05). The prevalence of AR children aged five to nine years was 13.80%, as aged ten to fourteen years was 19.72%. There was a significante differece between two groups (Χ2=4.056, P<0.05). The most explosive outbreak month in children with allergic rhinitis was July to September. The most common allergens found in patients were seasonal plants: mugwort, weed, dandelion, cereal, locust, ragweed, and willow. Fifteen point forty-six percent patients with AR also had rhinitic, 14.43% patients had asthma and 14.43% patients had allergic conjunctivitis. The history of drug allergy accounted for 12.37% in patients with AR, and food allergy accounted for 26.80%. In allergic rhinitis risk factors, family history accounted for 30.92%, non-breastfeeding representing accounted for 34.02%, animal feeding history accounted for 19.59%; exposure to passive smoking accounted for 42.26%; domestic decoration accounted for 12.37%. There were significante difference between AR children and normal in family history and non-breastfeeding (P<0.05). CONCLUSION The prevalence rate of AR in children aged from 5 to 14 in Yinchuan is 14.45%. Strategy of prevention and treatment of AR should be worked out according to the epidemic feature of AR.
Adolescent ; Allergens ; Animals ; Asthma ; epidemiology ; Child ; Child, Preschool ; Conjunctivitis, Allergic ; epidemiology ; Female ; Humans ; Male ; Pollen ; Prevalence ; Rhinitis, Allergic ; epidemiology ; Risk Factors ; Surveys and Questionnaires

OBJECTIVE: To discuss the method and opportunity of operation for orbito-cranial fibrous dysplasia, and further to summarize our experience in repairing the bony defect with titanium after excision and assess the surgical outcomes. METHODS: A retrospective study was performed in 21 patients with visual function damage and/ or orbital malformation, who treated surgically. All patients were underwent CT examinations (coronal, horizontal, sagittal and three-dimensional scans) before surgery. CT image data of patients with serious orbital skull lesions were analyzed by computer to produce the three-dimensional, solid titanium mesh prostheses used to repair the lesions; for smaller lesions solid titanium mesh prostheses were shaped artificially during surgery according to the normal orbit and the cranium. All the patients were treated surgically via craniofacial approach. After removing the diseased tissue, the defective area was repaired by titanium plate and nets. RESULTS: Of the 21 patients, tumor removal was complete in 18 patients, and incomplete in 3 patients due to extensive invasion into the sious cavernosus. Of the 13 patients with vision impairment before surgery, vision was improved in 11 cases (range from 3 to 5 lines) following surgery. All orbital malformations were healed in these 11 patients. CONCLUSION Patients with fibrous dysplasia should undergo surgical treatment as soon as possible. Where this results in visual dysfunction or orbital-cranial deformities, repairing the bony defect with titanium material has many advantages: it is solid, easily molded, and easily fastened.
Adolescent ; Adult ; Aged ; Female ; Fibrous Dysplasia of Bone ; surgery ; Humans ; Male ; Middle Aged ; Orbit ; Prostheses and Implants ; Reconstructive Surgical Procedures ; methods ; Retrospective Studies ; Skull ; Titanium ; Young Adult

Objective:To investigate the expression of transmembrane 4 super family 1 (TM4SF1)in breast cancer tissue,and to elucidate its clinical significance and explore the related molecular biological mechanisms. Methods:A total of 190 cases of human breast cancer,110 cases of paracancerous tissue and 110 cases of normal breast tissue were collected.Immunohistochemistry was used to detect the expression levels of TM4SF1 mRNA in breast cancer tissue,paracancerous tissue,and normal breast tissue;Western blotting method was used to detect the expression levels of TM4SF1 in breast cancer tissue,paracancerous tissue,and normal breast tissue;RT-PCR method was used to detect the expression levels of TM4SF1 mRNA in breast cancer tissue,paracancerous tissue, and normal breast tissue.The positive expression rates of TM4SF1 in breast cancer tissue of the breast cancer patients with different clinicopathological features were detected.Results:The positive expression rate of TM4SF1 in the breast cancer tissue was significantly higher than those in paracancerous tissue and normal breast tissue (P <0.05);there was no significant difference in the positive expression rates of TM4SF1 between paracancerous tissue and normal breast tissue (P = 0.531);the expression of TM4SF1 was not correlated with age,but was closely correlated with tumor size,differentiation degree,lymph node metastasis and tumor stage (P <0.05);the positive expression rate of TM4SF1 in basal like breast cancer tissue was higher than those in the other three types of tissues (P <0.05).The results of Western blotting showed that the expression level of TM4SF1 in breast cancer tissue was higher than those in paracancerous tissue and normal breast tissue (P < 0.05 ), but there was no significant difference in the expression level of TM4SF1 between paracancerous tissue and normal breast tissue (P >0.05). The results of RT-PCR showed that the expression level of TM4SF1 mRNA in breast cancer tissue was higher than those in the paracancerous tissue and normal breast tissue (P <0.01);there was no significant difference in the expression level of TM4SF1 mRNA between paracancerous tissue and normal breast tissue (P > 0.05 ). Conclusion:The TM4SF1 is highly expressed in breast cancer tissue. TM4SF1 may affect the occurrence, development and distant metastasis of breast cancer through various mechanisms.TM4SF1 may be a potential target for the treatment of breast cancer.

Objective:For tympanosclerosis patients with ossicular chain fixation, we use ossicular chain bypass technique and evaluate its long-term effects. Methods:From June 2017 to June 2019, 147 patients with tympanosclerosis who underwent middle ear surgery with otoscopy in Yinchuan First People's Hospital were reviewed. The subjects were divided into three groups according to the implemented operation plan, 51 cases in the ossicular chain mobilization group（OCM）, 56 cases in the ossicular chain bypass reconstruction group（OCB）, and 40 cases in the malleus-incus complex resection reconstruction group（MICR）. Through a three-year follow-up, the medium and long-term effects of different operation plans were compared and analyzed. Results:There was no significant difference among the three groups in the incidence of tympanic membrane perforation, delayed facial nerve palsy, and the dispatch and displacement of PORP. The incidence of tympanic membrane retraction pocket or cholesteatoma after operation in OCB group（0） was significantly lower than that in OCM group（11.76%） and MICR group（7.5%）（P<0.05）. At 12 months after operation, ΔABG of OCB group and MICR group were better than that in the OCM group（P<0.05）. At 36 months after operation, ΔABG of OCB group was better than that in the OCM group（P<0.05）, and there was no significant difference between OCB group and MICR group. The audiological performance of patients with epitympanic sclerosis（ETS） at 12, 24 and 36 months after operation was better than that of patients with posterior tympanosclerosis（PTS） and total tympanosclerosis（TTS）（P<0.05）. Conclusion:Compared with patients undergoing ossicular chain mobilization and malleus-incus complex resection for ossicular chain reconstruction, patients with tympanosclerosis undergoing bypass technique have better and stable hearing prognosis in medium and long term. This technique can effectively prevent the formation of retracted pocket and cholesteatoma in patients with tympanosclerosis after operation.
Humans ; Tympanosclerosis ; Ear Ossicles/surgery* ; Ear, Middle ; Malleus/surgery* ; Cholesteatoma ; Retrospective Studies ; Ossicular Prosthesis ; Treatment Outcome

Objective:To analyze and summarize the implementation effect of basic essential surgical training (BEST) course of laparoscopic skills over the past 10 years and the practical experience in updating course content and models.Methods:The pre-class assessment questionnaires, basic laparoscopic operation assessment results, and post-class assessment questionnaires of the students who participated in the BEST course of laparoscopic skills were collected. According to the period of the course construction, the students were divided into two groups, namely students who used the course of single training system in the early stage (traditional group) and students who used the course integrating a variety of training systems after the course model was updated in the later stage (test group). The two groups were compared for the scores of track circle moving, tunnel crossing, and high and low columns, as well as their subjective evaluation of course setting and implementation effect. The t-test, Wilcoxon test, or chi-square test was conducted according to the data type using SPSS 13.0. Results:The time for 150 traditional group students to complete track circle moving, tunnel crossing, and high and low columns was 1.08 min (0.81 min, 1.60 min), 2.20 min (1.60 min, 3.27 min), and 4.86 min (3.28 min, 6.36 min), respectively, while the time for 75 test group students to complete the three operations was 1.27 min (0.87 min, 1.83 min), 2.57 min (1.58 min, 4.07 min), and 4.35 min (2.90 min, 6.42 min), respectively, with no significant difference between the two groups ( P>0.05). In terms of students' subjective evaluation of the course, a higher percentage of the test group students were satisfied with classroom environment, teaching method arrangement, training equipment, training opportunities, helping clinical work, and meeting pre-class expectations than those in the traditional group. Conclusion:The constantly updated BEST course can ensure the training quality of trainees and obtain their higher satisfaction. The benefits of this course in clinical practice can be further verified through long-term follow-up of these trainees.

OBJECTIVES: To establish an experimental method for evaluating material permeability of type I collagen hydrogels. METHODS: Using BSA-FITC as an indicator, by combining BSA-FITC with PBS they were used as permeability media, and using transwell load hydrogen sample to detect BSA-FITC transparent rate. RESULTS: In the concentration range of 100 μg·mL~0.781 μg·mL, the standard curve ≥ 0.99, Lower Limit of Quantity (LLOQ) is 3.125 μg·mL, RSD <5%, detection recovery rate is in the range of 80%~120%. CONCLUSIONS In this study, we established an experimental method for evaluating material permeability of hydrogel. The BSA-FITC transparent rate of type I collagen hydrogel was 100% at 28 h.
Collagen Type I ; chemistry ; Hydrogels ; chemistry ; Materials Testing ; Permeability

Objective:To investigate the distribution of allergens in patients with allergic rhinitis （AR） in Ningxia, and provide theoretical data for the prevention and treatment of AR in this region. Methods:A total of 1664 patients diagnosed with AR in the Otorhinolaryngology Head and Neck Surgery Department of Yinchuan First People's Hospital Outpatient Clinic from January 2018 to December 2021 were retrospectively collected. Use the allergen sIgE antibody detection kit （immunoblotting method） to detect inhalation and ingestion allergens in patients.Results: ①Among all AR patients, 1 158 cases were detected positive, resulting in the detection rate was 69.59%; ②The detection rate of inhalation allergen was 65.87%, and the detection rate of ingestion allergen was 19.83%; ③Mugwort was the most sensitive allergen, and 76.32% of the patients having a positive grade ≥3; ④Out of the patients, 294 cases （25.39%） were allergic to only one allergen, 244 cases （21.07%） were allergic to two allergens, and 620 cases （53.54%） were allergic to three or more allergens; ⑤During different seasons, the highest number of positive allergens detected was in the summer, with 968 cases （83.59%）. Mugwort was the main allergen during this season （69.01%）. After the COVID-19 epidemic, the total positive rate of sIgE tests in AR patients decreased compared to before, and the difference was statistically significant （P<0.001）; ⑥Mugwort, dog epithelium, mold combination, egg, peanut, soybean, Marine fish combination and fruit combination all showed statistically significant differences between different gender groups （P<0.05）; ⑦Common ragweed, mugwort, dust mite combination, cockroach, egg, milk, Marine fish combination, shrimp, fruit combination and nut combination all showed statistically significant differences among different age groups （P<0.05）; ⑧There were statistically significant differences in hay dust among different ethnic groups （P<0.05）. Conclusion:Artemisia argyi is the main allergen in Ningxia, and the distribution characteristics of different allergens are influenced by treatment season, the COVID-19 epidemic, gender, age, ethnicity, and other factors, showing certain distribution patterns and rules.
Allergens ; Artemisia ; COVID-19 ; Retrospective Studies ; Rhinitis, Allergic ; Skin Tests ; Humans ; Male ; Female

Objective : To investigate the molecular mechanisms by which SOX7 regulates the SHP-2 / Wnt / β-cate- nin / ROS pathway，affecting the proliferation，invasion，and migration of colorectal cancer cells． Methods : Twenty nude mice with subcutaneously transplanted tumor models were randomly divided into four groups : SOX7 NC (n = 5) ，SOX mimic (n = 5) ，SOX7 NC + PHPS1 (n = 5) ，and SOX7 mimic + PHPS1 (n = 5) to observe tumor growth. Human colorectal cancer cell line SW480 cells were transfected via lipofection and divided into six groups : SOX7 NC，SOX7 mimic，SOX7 NC + H2 O2 ，SOX7 mimic + H2 O2 ，SOX7 NC + PHPS1，and SOX7 mimic + PHPS1．The ex- pression of SHP-2 / Wnt / β-catenin / ROS pathway-related proteins in SW480 cells of each group was detected by Western blot．The invasion and migration capabilities of SW480 cells were assessed through scratch and Transwell invasion assays，while cell proliferation was evaluated using CCK-8 . Results : In vivo experiments demonstrated that tumors in the SOX7 mimic group were significantly smaller than those in the SOX7 NC group (P＜0. 01) ．Tumors treated with PHPS1 intervention exhibited a significant increase in volume．There was no statistical significance in the difference in tumor volume between the SOX7 mimic + PHPS1 group and the SOX7 NC + PHPS1 group．In vitro experiments revealed that SOX7 mimic inhibited the expression of Wnt，β-catenin，NOX2，NOX4，PI3K，P-PI3K， AKT，P-AKT proteins (P ＜0. 01) ，and promoted the expression of p-SHP-2 protein (P ＜0. 01 ) ．The addition of hydrogen peroxide and SHP-2 inhibitor reversed the effects of SOX7 on SW480 cells (P＜0. 05) ，and significantly promoted the expression levels of Wnt，β-catenin，NOX2，NOX4，PI3K，P-PI3K，AKT，P-AKT proteins，with no sig- nificant difference，while significantly reducing the expression levels of SHP-2，p-SHP-2 proteins，with no significant difference．PHPS1 inhibited the expression of SHP-2，p-SHP-2 proteins (P＜0. 05) and upregulated the expression of Wnt，β-catenin，NOX2，NOX4，PI3K，P-PI3K，AKT，P-AKT proteins (P＜0. 05) ．Scratch，Transwell invasion and migration assays，and CCK-8 experiments indicated that SOX7 suppressed the migration，invasion，and proliferation of SW480 cells through oxidative stress and the SHP-2 pathway (P ＜0. 01) ，while H2 O2 and PHPS1 intervention promoted the migration，invasion，and proliferation of SW480 cells (P ＜0. 05 ) ． Conclusion SOX7 can suppress the proliferation，invasion，and migration of colorectal cancer by targeting the SHP-2 / Wnt / β-catenin / ROS pathway.