Objective To determine the effect of peripheral nerve regeneration through NGF ·HAMM (Nerve growth factor and Human Ammion membrane Matrix) grafts. MethodsA 1.2 cm defect of the left sciatic nerve were made and then repaired randomly by means of autogenous nerve bridging (group N),NGF · HAMM microporous conduit bridging (group NH) or HAMM microporous conduit bridging (group H) in 60 adult Wistar rats. There were 20 animals in every group. At 1,2 and 3months post operation,the following items as qualitative histology,quatitative histomorphometry,electrophysiology and functional assessments of end-organ reconnection were determined. ResultsAt 2and 3 months post operation,Motor nerve conduction velocity (MNCV) of the nerve repaired with HAMM were lower than that of the nerve repaired with autograft and NGF · HAMM. At 3 months post operation,there was no significant difference between NH group and N group in nerve conduction velocity (P>0. 05). At 2 and 3 months post operation,both NGF · HAMM conduit and nerve autograft groups showed more nerve fibers,bigger axon diameter and thicker meylin sheaths than H group.Conclution The combination of HAMM and NGF world enhance nerve regeneration and myeliogeny,as well as could cooperatively promote the functional recovery of injured nerves and limbs. Our results indicate that the NGF · HAMM microporous conduit is a promising tool for use as a nerve guide tube in peripheral nerve regeneration.

Objective To investigate specific anti-leukemia immune response induced by tumor lysates pulsed dendritic cells(TP-DC)in syngeneic bone marrow-transplanted mice.Methods From June 2002 to June 2003,the bone marrow cells were induced by using granulocyte-macrophage colony stimulating factor(mGM-CSF)and interleukin-4(mIL-4).DC was pulsed with L7212 tumor lysates at the immature stage.TP-DC was harvested on 7th day.615 mice immunized thrice with L7212 TP-DC starting at day 7 following BMT.Additional control groups were set up.The survival was observed.Results Immunization using TP-DC could induce L7212 cells-specific CTL responses that were statistically significant compared with control groups(F=391.77,P

It is reported in this article that ?-lipoprotein and partial ?_1-and X_2-globulin in scrum are apparently lower, but haptoglobin and ?-lipoprotein are apparently higher by polyacryamide gel clectrophoresis in 50 cases of uremi(?) patients, and that albumin and partial ?_1-and ?_2-globulin in scrum are apparently lower, but partial ?_2-globulin and ?-lipoproteinare apparently higher by polyacryamide gel clectrophoresis in 50 cases of nephrotic syndrome patients. The mechanism and clinical significance of these changes are discussed.

Objective To study the clinical features, diagnosis and surgical treatment of thoracic malignant lymphoma. Methods Clinical data of 22 patients with malignant lymphoma were retrospectively analyzed. Among them 16 were classified as non-Hodgkin's lymphoma (B cell lymphoma 12 cases and T cell lymphoma 4 cases), and 6 were classified as Hodgkin's lymphoma (nodular sclerosing lymphoma 5 cases and 1 case of mixed cellularity type lymphoma). Most of the tumors were located in anterior mediastinum or middle superior mediastinum, and 2 of the tumors located in upper left lung, another 2 located in upper right lung. Cough, stethocatharsis, chest distress and chest pain were the main clinical symptoms. Abnormal masses in mediastinum were seen on X ray film, occasionally with changes in lung. Before the operation, 4 cases were misdiagnosed depended on the results of intracranial puncture examination, and the other 18 cases were misdiagnosed as either lung cancer with mediastinal lymph nodes metastasis or thymoma based on the imaging findings. All patients received either total tumor resection or partial excision. After the operation, 16 cases with non-Hodgkin's lymphoma received CHOP chemotherapy (2 cases received radiotherapy and 2 cases received autologous hematopoietic stem cell transplantation after chemotherapy), 6 cases with Hodgkin's lymphoma received ABVD regimen (2 case received radiotherapy). Results Fourteen cases with non-Hodgkin′s lymphoma (2 cases for stage Ⅰ, 4 cases for stage Ⅱ, 3 cases for stage Ⅲ, 5 cases for stage Ⅳ) had survived for 1-112 months until the follow-up, 6 cases with Hodgkin′s lymphoma (2 cases for stage Ⅰ, 1 case for stage Ⅱ, 1 case for stage Ⅲ, 2 cases for stage Ⅳ) had survived for 3-46 months. Two cases with non-Hodgkin′s lymphoma died 18 and 35 months after operation, respectively. Conclusions The preoperative diagnosis to thoracic malignant lymphoma is difficult for the special clinical features limited. Surgical excision is important for diagnosis and treatment of thoracic malignant lymphoma. Comprehensive treatment after surgery includes radiotherapy, regular chemotherapy and immunotherapy.

In this paper, the hardware and software designs of a portable CPR device are introduced. The system, with a DSP chip and a single chip microcomputer composing the control system, can perform CPR and monitoring at the same time. This paper expatiates on such contents as the signal acquisition and process, the drive of the touch screen, closed cardiac massage and the control of the respiration part.

A new nylon affinity matrix with L-tryptoph ane as ligand was prepared for adsorption of bovine γ-globulin. Effects of tem perature, ionic strength and pH on affinity adsorption of γ-globulin on affinity membrane were studied by batch method. The results show that the interaction between ligand and γ-globulin includes mainly electrostatic and hydrophobic interaction. The affinity adsorption at optimum condition obeys Langmuir adsorption model with maximum adsorption capacity and minimum nonspecific adsorption. The space location between protein and ligand and the configuration of proteins wi ll change when deviating from this condition.

Objective To observe the effect of H shaped decalcified bone grafting on spinal fusion and anti conglutination by posterior approach and to evaluate its values in the application in spinal posterior approach surgery. Methods Total laminectomy was performed on 23 cases of severe spinal stenosis and 31 cases of intraspinal tumor for decompression. Thorough decompression of the nerve roots was performed on the former following total laminectomy while exploration and excision of the neoplasm were done on the latter. Then, H shaped decalcified bone grafts were inlaid between spinous processes and transverse processes to cover the lamina defection and overlap the adjacent segments in all cases. At last, the spinal internal fixation or plaster external fixation were used to stabilize vertebral segments according to the economic status of patients. The average time of follow up was 21 months (18～31 months). Results All patients got successful segmental spinal fusion within 8 months. Clinical symptoms were obviously alleviated in 43 cases at the end of follow up but symptoms recurred 12 months after operation in other 11 cases(all were tumor patients). Conclusion H shaped decalcified bone graft is valuable in spinal fusion and anti conglutination in spinal posterior approach surgery.

Objective To detect the change of brain activity under different depth of anesthesia (DOA)noninvasively. Methods The Lempel-Ziv complexity C(n) was used to analyze EEG and its four components (delta,theta, alpha, beta), which was recorded from SD rats under different DOA. The relationship between C(n) and DOA was studied. Results The C(n) of EEG will decrease while the depth of anesthesia increasing and vice versa. It can be used to detect the change of DOA sensitively. Compared with power spectrum, the change of C(n) is opposite to that of power spectru,. Only the C(n) of delta rhythm has obvious variations induced by the change of DOA, and the variations of delta is as similar as the EEG's. Conclusion The study shows that the desynchronized EEG is replaced by the synchronized EEG when rat goes into anesthesia state from awake, that is just the reason why complexity and power spectrum appear corresponding changes under different DOA. C(n) of delta rhythm dynamic change leads to the change of EEG, and the delta rhythm is the dominant rhythm during anesthesia for rats.

Objective　To investigate the surgical management of a gun shot wound of blood vessels and immersied, and evaluate its primary effect. Methods　100 rabbits were divided randomly into simple wounded group(SWG,n=50) and seawater immersion group(SIG,n=50).F emoral arteries were impacted by 0.38 gram steel spheres with velocity of 600 ～800 meters per second fired by 7.62 mm rifle. Animals in SIG were immersed in artificial seawater (pH 8.2～8.4, salinity 25.4,temperature 21℃) for 60 min, o f which those in SWG were spared. Grossly injuried artery was excised and restor ation of blood flow was reconstructed by end-to-end anastomosis or reversed au togenous venous grafting or cryopreserved arterial allografting. At 24 h,7,1 4,21 days after operation, blood flow was examined by Doppler ultrasonic detecti on and part of anastomotic sites and graft were collected for pathological obser vation. Results　In completely transected injury, the patency in SIG was 80.00%,while that in SWG was 86.67% in the first 3 weeks. In arterial c ontused injury ,patency in SIG was 86.67%,and that in SWG was 82.35% at the same time. Thrombosis occurred mostly in the first postoperative week. Atypical endo thelial cells were found at the anastomosis sites in the first postoperative week, and the anastomosis sites were lined with endothelium in 3 weeks postopera tively. Conclusion　Early curative effect could be obtained. Whe n grossly injuried artery is excised and followed by a routine surgical procedur e in the treating gunshot wounds immersed in seawater.

Objective:To observe the effect of Er Chen Tang on CYP2E1 and mitochondrial energy metabolism in nonalcoholic fat-ty liver disease ( NAFLD) to explore the role of Pinellinae Rhizoma Praeparata ( PRP) and Citri reticulatae pericarpium ( CRP) in the treatment of nonalcoholic fatty liver disease. Methods:Er ChenTang and the prescription without PRP or CRP was respectively given the animal models by gastric gavage. The serum levels of ALT, AST, triglyceride, cholesterol, SOD and MDA in hepatic tissue, and the contents of liver tissue CYP2E1 and ATP were detected in the mice. Results:The CYP2E1 levels in NAFLD mice increased signif-icantly with abnormal mitochondrial energy metabolism. Compared with those in the model group, the levels of ALT, AST, triglyceride and cholesterol were significantly reduced by Er Chen Tang, meanwhile, the content of CYP2E1 was reduced and also restored liver en-ergy metabolism. The treatment effect significantly decreased when the lack of PRP or CRP, and the ability of restoring liver mitochon-drial energy metabolism of Er Chen Tang decreased significantly when the lack of PRP (P<0. 05). After the removal of CRP, the in-hibition ability of Er Chen Tang to CYP2E1 levels significantly decreased (P<0. 05). Conclusion:Er Chen Tang can effectively im-prove nonalcoholic fatty liver diseases, and effectively reduce the content of CYP2E1 in liver tissue of mice and restore the mitochondri-al energy metabolism.