Objective Effects of plasma VEGF,serum AFP and CRP levels in patients with primary hepatocellular carcinoma (HCC)change and correlation analysis.Methods using ELISA method,chemiluminescence method,immune transmission turbi-dimetry,in March 2010 to November 2012 in our hospital during the period of inpatient and outpatient clinic of 35 cases of primary hepatocellular carcinoma (HCC),33 cases of patients with cirrhosis,the plasma VEGF,serum AFP and the determination of the content of CRP and collect 32 normal healthy people at the same time the determination results of comparison.Results the plasma VEGF in HCC (primary hepatocellular carcinoma (group)and serum CRP and LC group (cirrhosis)and NC (healthy control group),the difference was statistically significant (P<0.01),there is no correlation between VEGF and AFP,the joint detection can improve the sensitivity of HCC diagnosis (91.6%)and accuracy (90.3%),plasma VEGF in patients with hepatocellular carci-noma group,serum AFP and the expression of CRP level is significantly higher than normal control group.Conclusion To detect the plasma VEGF,serum AFP and the change of CRP level,changes in patients with primary hepatocellular carcinoma (HCC)dis-ease process,has certain relevance and important clinical value.

Objective To study the echo and flow dynamic change of fetal lung during pregnancy. Methods Take advantage of ultrasound attenuation and Doppler effect to observe the echo and dynamic change of fetal lung.Results ① The echo of lung and the pulsatile index of intrapulmonary artery increased according to gestational ages.② Pathological pregnancy had no effect on the echo of lung,and diabetes had no effect on the flow indexes of intrapulmonary artery,either.Conclusions The echo and flow indexes of fetal lung have dose relation with gestational ages.

Objective Periodontal clinical effects of hyperbaric oxygen therapy were analyzed and discussed the foundation .Methods Our hospital from 56 patients with periodontal disease in August 2013 admitted -August 2014 period, were randomly divided into experimental and control groups , and the control group underwent drug treat-ment on a regular basis , the experimental group on the basis of the control group , the line hyperbaric oxygen therapy , compared to its clinical effects .Results The symptoms were improved , but the effect more pronounced in the exper-imental group, which was significantly better than the control group of patients , compared to significantly different sta-tistically significant ( p<0.05 ) .Conclusion Hyperbaric oxygen combined basis have better clinical treatment of periodontal disease , which should be widely applied , pending further improved and refined to improve the outcome .

OBJECTIVE:To observe the clinical efficacy and safety of Ligustrazine injection,α-ketoacid combined with levam-lodipine in the treatment of chronic renal failure. METHODS:92 patients with chronic renal failure were randomly divided into ob-servation group(46 cases)and control group(46 cases). All patients received electrolyte,vitamins and essential amino acid,low salt,low fat,hypoglycemic and hypolipemic,anti-infection,correcting anemia and other conventional symptomatic treatment. Based on it,control group received 8 Compound α-ketoacid tablets,3 times a day + 1 Benzenesulfonic acid levamlodipine tablet, once a day. Observation group additionally received 150 mg Ligustrazine hydrochloride injection,adding into 250 ml 5% Glucose injection,intravenous infusion,once a day. The treatment course for both groups was 3 months. Clinical efficacy,and urea nitro-gen (BUN),serum creatinine (Scr),serum cystatin (CysC),low-density lipoprotein cholesterol (LDL-C),total cholesterol (TC),triglyceride (TG) levels before and after treatment and the incidence of adverse reactions in 2 groups were observed. RE-SULTS:The total effective rate in observation group was significantly higher than control group,the difference was statistically sig-nificant(P<0.05). Before treatment,there were no significant differences in the BUN,Scr,CysC,LDL-C,TC and TG between 2 groups(P>0.05). After treatment,BUN,Scr,LDL-C,TC and TG in 2 groups were significantly lower than before,and obser-vation group was lower than control group,CysC was significantly higher than before,and observation group was higher than con-trol group,the differences were statistically significant (P<0.05). And there was no significant difference in the incidence of ad-verse reactions(P>0.05). CONCLUSIONS:Based on conventional treatment,Ligustrazine injection,α-ketoacid combined with le-vamlodipine show obvious efficacy in the treatment of chronic renal failure,they can improve the renal functions,with good safety.

Objective:To analyze the financial auditing method after changing the settlement method of outpatient medical income in hospitals under the background of launching “Jing Yi Tong” business line in public hospitals of Beijing.Methods:It summarized the key point of financing audit in outpatient medical income of hospitals under the settlement mode of “Jing Yi Tong”business,proposed the effective settlement method and suggestions on auditing.Results:Through close financial auditing,it effectively ensure the acuteness and completeness of outpatient medical income of hospitals,prevent the leak and man-made fraud in settlement process.Conclusion:The effective hospital financing audit was the main task for internal control,which played key role on improving hospital financing management level.

Programmed cell death 1 (PD-1) is an important negative costimulatory molecule discovered in recent years, which is expressed on the surface of T and B cells and plays an important role in the regulation of cellular immune response and immune tolerance.After the combination of PD-1 and its receptor programmed cell death 1 ligand 1 (PD-L1), negative regulatory signals are transmitted to suppress the immune response.Under the activation of stimulating factors, PD-1 and PD-L1 are combined to weaken the conduction of downstream signaling pathways such as PI3K/Akt and ERK through recruitment of phosphorylated SHP2, thereby inhibiting the proliferation of T cells and the production of cytokines, inhibiting the immune response and participating in the occurrence and development of a large number of inflammatory diseases.PD-1 in the field of ophthalmology research is still in its infancy.As far as we know, PD-1 participates in ocular inflammatory diseases such as uveitis, sympathetic ophthalmia, allergic conjunctivitis, also is involved in corneal transplant rejection, optic nerve crush injury and optic myelitis, diabetic retinopathy, thyroid related ophthalmopathy, melanoma and other diseases, thus preventing the interactions between PD-1 and its receptor PD-L1 may become a new potential target for the treatment of ocular tumor, inflammation, autoimmune and neurodegeneration disease.In this article, the latest research progress of PD-1 and its receptor PD-L1 in the pathogenesis of ocular diseases were reviewed.

Objective To use the genetic diagnosis technique to perform the gene detection in the patients with thalassemia for understanding the main gene mutation types and their gene frequencies .Methods Totally 5 500 outpatients and individuals un‐dergoing physical examination in the hematology department of the Huizhou Municipal Central People′s Hospital from January 2010 to October 2014 were taken as the research subjects .The GAP‐PCR and membrane reverse hypridition technology were adopted to conduct the gene analysis of α‐andβ‐thalassemia .Results A total of 1 604 cases ofα‐globin gene change were detected ,accounting for 29 .16% of the total detected subjects ;1 096 cases ofβ‐globin gene change were detected ,accounting for 19 .93% of the total de‐tected subjects ;119 cases of αβ‐complex thalassemia were detected .Conclusion The gene screening of α‐ and β‐ thalassemia pro‐vides the valuable basic data for conducting the marital and fertility instructions .

Objective: To observe the anemia situation and pregnancy outcome of pregnant women with iron deficiency anemia ( IDA) treated with polysaccharide iron complex. Methods:Totally 102 patients with IDA were randomly divided into the control group (51 cases) and the study group (51 cases). The control group was treated with iron dextran tablets orally, and the study group re-ceived polysaccharide iron complex. The improvement of anemia and the pregnancy outcome were compared, and the adverse reactions were recorded. Results:After the treatment, hemoglobin ( Hb) , serum iron ( SI) , serum ferritin ( SF) and serum transferrin receptor ( sTfR) at 24-week pregnancy and before the delivery, the red cell count ( RBC) and mean corpuscular hemoglobin ( MCH) before the delivery in the study group were all better than those in the control group (P<0. 05). The incidence of adverse reactions in the two groups was similar. Conclusion:Polysaccharide iron complex can improve anemia status of pregnant women with IDA, which is worthy of widely clinical use.

Protein phosphatase 2A(PP2A)is the main serine/threonine protein phosphatase of the brain,and it is involved in many physiological and pathological processes in the cell. Particularly in the pathogenesis of Alzheimer′s disease(AD),PP2A is closely related to the main pathological features of AD. Deregulation of PP2A enzymes correlates with increased tau phosphorylation, the formation of amyloid precursor protein,and the missing of neurons. PP2A as the target of drug development may bring new hope for the treatment of AD. This review introduces the structure and activity regulation of PP2A,and the role of PP2A in AD.

Objective To compare the clinical value of two kinds of imunoassay method including CLIA and ELISA in the detection of related antibody of patients with HCV.Methods 60 patients with HCV were chosen as study subjects,and the related antibodies of patients were detected by CLIA and ELISA.The positive rates of anti HCV antibody,anti-3E antibody,anti-GP210 antibody,anti-SP100 antibody,anti-PML antibody and anti-AMA -M2 antibody detected by CLIA and ELISA were compared.Results The positive rates of anti-HCV antibody detected by CLIA and ELISA were 83.33％ (50/60),98.33％ (59/60),respectively.The anti-HCV antibody detected by CLIA was significantly higher than by ELISA (x2 =4.73,P =0.03).The positive rates of anti-3E antibody,anti-GP210 antibody,anti-SP100 antibody,anti-PML antibody and anti-AMA-M2 antibody detected by ELISA were 11.67 ％ (7/60),6.67 ％ (4/60),11.67 ％ (7/60),10.00 ％ (6/60),11.67 ％ (7/60),respectively,those detected by CLIA were 73.33 ％ (44/60),50.00％ (30/60),38.33 ％ (23/60),58.33 ％ (35/60),80.00％(48/60),respectively.The positive rates of anti-3E antibody,anti-GP210 antibody,anti-SP1O0 antibody,anti PML antibody and anti-AMA-M2 antibody detected by CLIA were significantly higher than by ELISA(x2 =18.81,11.51,13.23,15.08,13.75,all P＜0.05).Concelusion Compared with ELISA,CLIA in the detection of related antibodies of patients with HCV can efficiently higher the positive rate of anti-HCV and autoantibody.