Animals ; Autophagy ; genetics ; Genes ; Humans ; Neoplasms ; genetics ; pathology ; Signal Transduction ; TOR Serine-Threonine Kinases ; metabolism

ObjectiveTo discuss the application of medical elastic stockings in nursing of patients with deep venous thrombosis formation. Methods62 patients diagnosed as acute lower limbs deep ve-nous thrombosis from January, 2005 to January, 2008 were randomly divided into the experimental group and the control groups with 31 patients in each group, the control group took general nursing measures, the experimental group used the medical elastic stockings based on the general care measures, The inci-dence of post- thrombosis syndrome was observed in two groups and underwent χ2 test. ResultsAfter two years follow- up, 2 patients relapsed and 3 patients appeared post- thrombosis syndrome in the experimental group, 8 patients relapsed and 10 patients had post-thrombosis syndrome in the control group. The relapse rate and incidence rate of peat-thrombosis syndrome in the control group were higher than those of the experimental group. ConclusionsThe use of medical elastic stockings can prevent acute lower limbs deep venous throm-bosis, and can reduce the symptoms of post-thrombosis syndrome, which deserves to be spread widely.

OBJECTIVE: Dicerl plays an important role in generation of microRNA, the purpose of this study was to evaluate Dicerl expression and its prognostic value in nasopharyngeal carcinoma (NPC). METHOD: The protein expression of Dicerl was examined by immunohistochemistry in 276 NPC specimens, and the mRNA levels of Dicerl were analyzed by qRT-PCR in 56 NPC and 11 nasopharyngitis tissues. Cox regression analysis was used to identify independent prognostic factors, and a prognostic score model was constructed for survival prediction. RESULT: Expression of Dicerl was downregulated in NPC tissues at both the mRNA and the protein levels, and there was a notable positive correlation between the expression levels of Dicerl mRNA and protein. Low Dicerl expression was positively correlated with distant metastasis (P<0. 01) and death (P<0. 05). In addition, low expression of Dicerl was significantly associated with poorer overall survival (HR = 2. 32, 95% CI: 1. 30 ~ 4. 14, P<0. 01) and poorer distant metastasis-free survival (HR = 2. 56, 95% CI: 1. 39 ~ 4. 74, P<0. 01). Furthermore, multivariate analysis showed that low expression of Dicerl and tumor-node-metastasis (TNM) stage were independent prognostic indicators for NPC patients. A prognostic score model combining the Dicerl expression and TNM stage had a better prognostic value than the TNM stage alone model or Dicer) expression alone model (P< 0. 05). CONCLUSION Dicerl was downregulated in NPC tissues at both the mRNA and the protein levels, and low expression of Dicerl could be served as novel prognostic biomarker for NPC patients.
Carcinoma ; DEAD-box RNA Helicases ; biosynthesis ; Humans ; Immunohistochemistry ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; metabolism ; pathology ; Prognosis ; Proteomics ; RNA, Messenger ; Ribonuclease III ; biosynthesis

Objective:To observe the anatomic characteristics of supratubal recess and the morphological change of it in the sick ear. To investigate the relation between the morphological changes of supratubal recess and otitis media.Method:Using high resolution CT to observe and measure the supratubal recess of the normal ears and the ailing ones.Result:For the normal adults, the anteroposterior diameter of the supratubal recess is(3.83±1.28)mm,the vertical diameter(3.67±1.14)mm,the height(3.76±0.36)mm;and the length of the cog is(2.84±0.38)mm.The size of the supratubal recess showed no significant difference between left and right(P＞0.05).There was no relationship between the size of the supratubal recess and pneumatization of mastoid cells (P＞0.05).There was significant differences in size of the supratubal recess between normal ears and the ears with cholesteatoma(P＜0.05),but there was no relationship between the size of the supratubal recess and pneumatization of mastoid cells(P＞0.05)in the ears with cholesteatoma.There was no significant differences in size of the supratubal recess between normal ears and the ears with chronic otitis media(P＞0.05).In the ears with cholesteatoma,40% had osseous destruction in supratubal recess.Conclusion:For the normal adults, the size of the supratubal recess showed no significant difference between left and right, and there was no relationship between the size of the supratubal recess and pneumatization of mastoid cells. Anterior tympanic isthmus , posterior tympanic isthmus and supratubal recess which is the supplement of the formers constitute the air way between attic and mesotympanum. The supratubal recess of the cholesteatoma is smaller than the normal, when the eustachian tube dysfunction occurs, the air way in the middle ear would herein be affected, and cholesteatoma forms eventually. There was no significant differences in size of the supratubal recess between normal ears and ears with chronic oti-tis media, as in the clinic.The effect of the supratubal recess should be taken into account when we consider the pathogeny and treatment of the middle ear cholesteatoma.

Objective To study the protective role against cisplatin-induced ototoxicity by using radix astra-gali in rats. Methods Forty rats were randomly divided into four groups. Group A received saline as controls. Group B received radix astragali. Group C received injection of cisplatin(4 mg/kg) as experiments for 6 days. Group D received both radix astragali (5 g/kg) and cisplatin (4 mg/kg). Distortion product acoustic emission (DPOAE) was applied to each rat before and 7 days after cisplatin injection. All the animals were sacrificed on the 7th day. Half of the cochleas were observed by frozen section and the apoptosis of hair cells was detected by TUNEL meth-od. Scanning electron microscopy was utilized to evaluate the cochlear morphology of the other rats. Results The DPOAE amplitudes of Group C decreased significantly compared to the group A and group B(P<0.01). The dam-age and apoptosis of hair cells were noted in the group C and group D, while the hair cells of group A and group B showed no sign of apoptosis or damage. Compare to experimental group , the DPOAE amplitudes of group D were higher, and the damage and apoptosis of hair cells were significantly lower(P<0.05). Conclusion This study sug-gests that radix astragali can effectively reduce cisp[atin ototoxicity.

Objective The study focused on the relationship between neopterin (NP),Gensini score and high-sensitivity C-reactive protein (hs-CRP) to explore the important role of the neopterin in patients with coronary heart disease.Methods Total of 442 patients,admitted to the Department of Cardiology at Renmin Hospital,Wuhan University from January 2012 to September 2012,were enrolled in this study.There were 176 patients in the control group and 266 patients with coroary disease in the experimental group.The Gensini score was used to assess the severity of the coronary lesions in the patients with coronary heart disease.The enzyme-linked immunosorbent assays (ELISAs) were used to measure the serum NP level.The other indicators were assessed by use of fully automatic biochemical and coagulation analyzers.The data were analyzed by using of SPSS19.0.T-test was used for analysis of the results between the control group and the experimental group.One-factor analysis variance (ANOVA) was used for comparisons of the results among the 3 groups,and Spearman correlation analysis was used for the correlation analysis between the NP level and Gensini score as well as hs-CRP level.Results The serum NP level was higher in the experimental group than that in the control group [(1.99 ± 0.37) vs (1.48 ± 0.29) ng/ml,P ＜0.05],and the NP level was positively correlated with the severity of coronary heart disease.Compared with the stable angina (SA) group,the serum NP level was significantly increased in the unstable angina (UA) group (2.00 ± 0.37) ng/ml and the acute myocardial infarction (AMI) group (2.10 ± 0.43) ng/ml (t =2.38,P ＜ 0.05).In addition,the serum NP level was positively correlated with the Gensini score (r =0.687,P＜0.001) as well as with the level of hs-CRP (r=0.190,P＜0.001).Conclusions The serum level of NP was significantly higher in patients with coronary heart disease and was positively correlated with the severity of coronary artery disease.Thus,NP may become a new indicator for the assessment of the inflammatory response in coronary atherosclerosis.

Patients with nasopharyngeal foreign body sensation for 3 years, and had nasal obstruction in the past six months. electric nasopharyngoscopy: a irregular ellipse shape mass occupied in the nasopharynx, the mass surface is not smooth, with erosion ulcer and filthy secretions, the mass had a root in the back-end of nasal septum, and was adjacent to the bilateral round pillow. Sinus CT showed an irregular soft tissue shadow connected to the nasal septum backend in the nasopharynx, the size is about 2.8 cm X 3.5 cm, CT value is about 43 HU. Pathological examination: papillary adenocarcinoma.
Adenocarcinoma, Papillary ; diagnosis ; surgery ; Endoscopy ; Humans ; Nasal Obstruction ; Nasal Septum ; pathology ; Nasal Surgical Procedures ; Nasopharynx ; Tomography, X-Ray Computed

A male of 17 years old complained of bilateral nasal congestion with mouth breathing for half a year. The physical examination showed patency of bilateral nasal cavity. Nasopharyngeal fiberscope revealed a huge spherical smooth reddish mass at the nasopharyngeal posterior wall and the back-end of nasal cavity blocking the entire postnaris and contacting with bilateral tubal tori, the size of which is about 3.5 cm x 2.5 cm. The nasopharyngeal 3D-CT showed a low density cystic mass area in the nasopharynx with smooth edges, and the CT value is approximately 32.4 HU. No exact enhancement was observed. The cystic mass originated from and compressed the back end of nasal septum. A semicircular defect in the leading edge of clivus was observed, and the inferior wall of sphenoid sinus is compressed uplifted upwards. The nasopharyngeal MR showed that the nasopharyngeal lesion presented short T1 and long T2 signals with multiple small wall nodules around. Neither the lesion nor the peripheral nodules can be significantly enhanced by enhancement scan. After a nasal endoscopic surgery, the case was definitely diagnosed as nasal septum back-end huge cyst.
Adolescent ; Cysts ; Humans ; Male ; Nasal Septum ; pathology

OBJECTIVE To discuss the gentamycin-induced ototoxity and mechanism on cochlear outer hair cells. METHODS All the guinea pigs were randomly divided into 3 groups. Gentamycin, gentimycin plus furosemide and normal saline were intraperitoneally injected for 6 days respectively. The amplititude of distortion product otoacoustic emissions (DPOAE)urface prepration of cochlear basilar membrane, hair cells’ apoptosis and expression of caspase-3 after injection were analyzed. RESULTS Reduction of the DPOAE amplitude, hair cell damage, apoptosis and Caspase-3 expression were observed in group injected with gentamycin as well as in group injected with gentamycin plus furosemide. CONCLUSION Gentamycin should induce hair cell damage in the cochlea of guinea pigs and Caspase-3 plays an important role in the process of hair cell apoptosis.

Ninety six offspring infants (48 males, 48 females) born by hospitalized patients with intrahepatic cholestasis of pregnancy from January 2010 to February 2013 ( study group) and 108 cases of offspring infants (54 males, 54 females) born by hospitalized healthy pregnant woman ( control group) were enrolled in the study. All offspring infants were followed up for 2 years after birth, physical and neuropsychological development parameters were investigated at 1 month, 1 year and 2 years after birth. There were no significant difference in body weight [ ( 11.94 ±0.89 ) vs.( 13.99 ±0.78 ) kg ] , length [(86.2 ±2.0) vs.(87.0 ±3.1) cm], sitting height, [(51.8 ±2.1) vs.(51.8 ±1.9) cm], head circumference[(47.9 ±1.3) vs.(48.1 ±1.1) cm], development of intelligence quotient index[(99.4 ± 12.9) vs.(100.0 ±12.0)] at 2 years after birth between two groups (P>0.05).Two-year follow up reveals that ursodeoxycholic acid and S-adenosine methionine treatment for women with intrahepatic cholestasis of pregnant has no significant adverse effect on the growth and development of their offspring.