Objective To compare the ability of intentional forgetting to emotional words of post traumatic stress disorder(PTSD) children and normal children by item method after Wenchuan earthquake 18 months.Methods Twenty PTSD children who met with CCMD-3 and DSM-Ⅳ criteria and twenty control group children were investigated. Sixteen positive and sixteen negative words were adopted as materials. PTSD children accomplished the words learning, arithmetical operation and memory tests ( include free recall, words completion and words recognition) in orderly. Results ( 1 )The free recall accuracy of PTSD children to positive words was significant high than negative words( (0.32 ±0.11 ), (0.28 ±0.10), P＜0.05). The free recall accuracy of control group to positive words was significant high than negative words ( (0.37 ± 0.09 ), (0.34 ± 0.13 ), P＜ 0.05 ). ( 2 )The words completion accuracy of PTSD children to positive words was significant low than negative words( (0.38± 0.12 ), (0.49 ± 0.12 ), P ＜ 0.05 ). The words completion accuracy of control group to positive words was significant low than negative words ( (0.39 ± 0.15 ), (0.56 ± 0.14 ), P ＜ 0.01 ). ( 3 ) There was no difference of words recognition accuracy of PTSD children to positive and negative words ( ( 0.64 ± 0. 19 ), (0.63 ± 0. 16), P ＞0. 05). There was no difference of words recognition accuracy of control group to positive and negative words ((0.64 ± 0. 16), (0.65 ±0. 19), P ＞ 0. 05). Conclusion There is a significant intentional forgetting effect on emotional words in PTSD children and normal children, and this effect were influenced not only by selective rehearsal but also retrieval inhibition.

Objective: To observe the clinical effects of scalp acupuncture and body acupuncture on post-stroke ataxia. Methods: 124 cases of post-stroke ataxia were treated with scalp and body acupuncture to observe the changes of blood velocity in the blood vessels of the brain and the total effects after treatment. Results: The total effective rate was 93.5% based on the result of Berg Balance scale, and was 91.1% on the activities of daily living (ADL) index. The blood velocity of the vertebrobasilar artery was higher after the treatment compared to before treatment (P＜0.05). Conclusion: Acupuncture had good clinical effects.

Objective To investigate the characteristics of attentional control between Wenchuan earthquake anxious and normal children. Methods Using Screening Child Anxiety Related Emotional Disorders (SCARED), 18 earthquake anxious children were chosen as participants and 18 normal children were chosen as control group. They were asked to perform a visual search task. Results ( 1 ) The judgment accuracy of earthquake anxious children was lower than normal children's significantly ( ( 0.95 ± 0.01 ), ( 0. 98 ± 0. 01 ), P ＜0.01 ). ( 2 ) The reaction time of earthquake anxious children was significantly longer than normal children' s ((1664.5 ± 78.5 ) ms, ( 1110.7 ± 78.5 ) ms, P ＜ 0. 01 ). ( 3 ) There was significantly faster performance in the valid condition than in the neutral( ( 1304.7 ± 61.3 ) ms, ( 1382. 3 ± 47.4 ) ms, P ＜ 0. 05 ) and invalid condition ( ((1304.7 ± 61.3 )ms, (1475.8 ± 71.5 )ms; P ＜ 0. 05 ), as well as faster performance in the neutral than in the invalid condition ((1382.3 ± 47.4) ms, ( 1475.8 ± 71.5 ) ms, P ＜ 0.05 ) in all children. Conclusion The performance of earthquake anxious children on top-down attentional control task is less than normal children. It indicates that anxious emotion which is brought by traumatic incidents like earthquake influents earthquake anxious children' s cognitive processing ability.

Objective:To measure the real temperatures on the pluggers of three continuous-wave de-vices, and to provide theoretical reference to evaluate thermal damage and heat ’s influence on the filling materials.Methods:The dual channel K type thermocouple was contacted to various sizes ’ pluggers in three different continuous-wave devices (BeeFill, Elements, B&L), and the highest temperatures at dif-ferent points ( tip, and 2 mm, 5 mm, 10 mm from the tip) of the pluggers ( preset temperature was 200℃) were recorded.The measurements were performed 5 times.T-test was used to compare the real tem-peratures at the tips with that set on the display and one-way ANOVA was used to compare the tempera-tures of the pluggers in different devices , sizes and points .Results:The highest temperature was at the tip of BeeFill 40/0.03 plugger (198.7 ±7.7) ℃, but there was on statistical differences between that and the preset temperature 200℃.The temperatures of the remaining pluggers were obviously lower than 200 ℃(P<0.05).The lowest temperature of the pluggers was detected at 10 mm from the tip of BeeFill 60/0.06 plugger (69.9 ±4.0) ℃.The highest temperature of each plugger was detected at the tip or 2 mm from the tip (112.1 to 198.7℃,and the median was 140.8℃).Conclusion:The real temperature of most continuous-wave pluggers included in this study is below the set temperature 200 ℃.

Objective To study calcium chelator BAPTA-AM antagonize the cellular oxidative stress induced by hydrogen peroxide ( H2 O2 ) and to explore the effect of calcium ion on the cell degeneration mediated by NLRP3.Methods The SHSY5Y cell model of oxidative stress was made by hydrogen perox-ide,then the cell model was treated with calcium ion carrier A23187 or BAPTA-AM,a higher efficiency cal-cium chelating agent.The cells were divided into 4 groups:H2O2 treatment group,H2O2+A23187 group, H2 O2+A23187 +BAPTA-AM group and control group.NLRP3 protein was detected by Western blot,and Caspase-1 and IL-1βwere detected by ELISA.Results NLRP3 expression was significantly increased in cells treated by hydrogen peroxide(P<0.05) .The NLRP3 protein continued to increase, and the expression of Caspase-1((57.1±19.2)pmol/L) and IL-1β((484.2±49.5)pg/ml) protein was also increased signifi-cantly in cells treated by A23187,and the difference had statistically significant for caspase-1 or IL-1βin H2O2+A23187 group compared with those in control group(Caspase-1:(26.8±12.9)pmol/L,IL-1β:(326.9 ±52.1) pg/ml) (P<0.05, P<0.01) .NLRP3,Caspase-1 and IL-1βwere all significantly reduced after adding a high-er efficiency calcium chelating agent BAPTA-AM.Conclusion Calcium overload is likely to enhance the oxidative stress induced by hydrogen peroxide and engender neurodegeneration mediated by NLRP3 inflammasome.

Objective To investigate the relationship between job burnout and psychological health diathesis of the senior medical workers in Tianjin.Methods 263 medical workers were tested by Psychological Health Diathesis Scale (PHDS) and Chinese Maslach Burnout Inventory (CMB[).Results (1) Total detection rate of burnout was 69.3％.Detection rate of mild burnout was 43.0％,moderate 20.2％,and severe 6.1％.(2) Explain rate of self-concept and emotionality to the job burnout was 31.5％.Conclusion Job burnout is prevalent in the senior medical workers in Tianjin.Emotionality,interpersonal diathesis and self-concept,to some extent,can forecast job burnout.

Objective To explore the effect and mechanism of TGF beta1/smad3 signaling pathways on apoptosis in mouse pulmonary fibrosis.Methods Fifty-four healthy male C57BL/6 mice were randomly divided into three groups:normal control (n=18),pulmonary fibrosis model (n =18) and TGF-β1/smad3 inhibitor group (n=18).Six mice in each group were randomly killed on days 7,14 and 28.Hematoxyli~eosin and Masson staining were adopted to evaluate the severity of pulmonary inflammation and fibrosis.The content of hydroxyproline (Hyp) in the lung tissues was detected by alkaline hydrolysis technique.The apoptosis was observed by tunnel apoptosis assay kit.P-smad3 and caspase3 protein expressions were assessed via Western blot.Results Lung in model mice versus normal control showed alveolar inflammatory change in 7 days and significant pulmonary fibrosis in 28 days(P＜0.05).Meanwhile,apoptosis index,hydroxyproline content,caspase3,and phosphorylated Smad3 were obviously higher in model mice than in control group (P ＜ 0.05).Compared with model group,TGF-β1/smad3 inhibitor group showed that alveolitis and pulmonary fibrosis degree,hydroxyproline content,cell apoptosis index,the expressions of p-smad3 and caspase3 were decreased at same time point (P ＜ 0.05).Conclusions TGF beta1/smad3 signaling pathways may participate the abnormal apoptosis during the development of pulmonary fibrosis,and TGF-β1/smad3 inhibitor SB431542 could inhibit this process.

Objective To summarize the experience on diagnosis and treatment of splenic neoplasm. Methods Clinical data of 40 patients with primary splenic space occupying lesion treated in our hospital were retrospectively analyzed. Results Thirty four out of the 40 cases were diagnosed as primary splenic neoplasm preoperatively. The detectable rate of B-US was 94%, and that of CT was 96%. The discrimination rate of benignancy and malignancy by CT was 84%. Serum AKP and ?-GT were significanfiy increased in most of the malignancies. Seventeen among 22 cases with benignancy were treated by splenectomy, and the others underwent partial splenectomy or tumor resection. Sixteen of 18 patients with malignancy underwent splenectomy, and two did biopsy. Pathology revealed cysts in 13, angiocavernoma in 4, inflammatory pseudotumor in 3, caverous lymphangioma in 1, and cystic degeneration of liomyoma in 1; malignant lymphoma and lymphsarcoma in 9, hemangiosarcoma in 3, and fibrosarcoma, liomyosarcoma, malignant fibrous histiocytoma in 1 each. Three cases with malignancy have survived more than 5 years. Conclusions Splenic neoplasm is diagnosed mainly according to clinical manifestation and image examination. The discrimination of benignancy and malignancy depends on CT, angiography, and serous AKP and ?-GT level. Radical operation and complex treatment could improve the prognosis of splenic malignant tumor.

Hepatitis C virus (HCV) infects more than 200 million people globally and its increasing incidence is placing a heavy economic burden on resource-poor countries.Effective HCV diagnosis is of guiding significance for treatment selection and monitoring.Techniques for detection of HCV infection include serological and nucleic acid detection.Serological assay is simple and easy to operate for initial HCV diagnosis.However,the window phase is long,so the active phase of the virus or previous infection cannot be determined.Nucleic acid detection has high sensitivity and wide linear range,which is the direct evidence to determine virus infection.The traditional thermal cycle-based nucleic acid amplification technology has been widely used in clinical laboratories.The development of isothermal nucleic acid amplification technology overcomes the limitations of traditional amplification technology,and it can be used as a tool for Point-of-care Testing (POCT),which has great potential and particularly suitable to be used in low-resource areas with high HCV prevalence,such as Africa and Southeast Asia.In this paper,we outline the current advance and development trends in the HCV molecular diagnosis.

Picrotoxin is an anatagninst of gamma-aminobutyric acid, which is an internal inhibition-transmitter in the central nervous system, Picrotoxin exerts a biphasic action on the blood pressure and heart rate in rats and cats in vivo. That is to say, in the initial stage, picrotoxin can lower the blood pressure and heart rate, and then an elevation of these two even above the original level can be observed, up to the present, from the authors limited literature, there has been no report dealing with the problem whether picrotoxin can act on an isolated heart directly.In this study, the heart of a frog was isolated and routine intubation of the heart was done for its perfusion. Physiological polyconduction instrument was inserted through a mechanical transducer to record the heart rale and myocardial contractility. A suspending glass microelectrode coupling with a microamplifier is used to record the action potential of the ventricular myocardium. Real time analysis of all the data was accomplished with a microcomputer. The dosages of picrotoxin used were 1,5, 3.0, 6.0, and 12.0 mg per kilogram of body weight.It was found that picrotcxin can directly act on the isolated frog heart. The results were as follows.1 ) Picrotoxin exerts inhibition on the special conduction system of the heart,and the A-V node and venous sinus are very sensitive. Complete or partial transmission block can be induced.2 ) It can elicit clearly a fall of the heart rate but no biphasic action can berevealed. 3) It can reduce the myocardial contractility, suggesting that the calciuminflow during the functioning period of the action potential is effected. 4 ) It can reduce the amplitude of the action potential but no effect on themaximal depolarization speed is observed, suggesting that picrotoxin islikely to affect the level of resting potential but not the action potentialin the depolarized period.