0.5.8 factors can explain the 80% of the whole variable. There were significant differences in the aspects of emotion,ADL/IADL,moving ability,participating and the total score(P

Objective To design standardized cases to evaluate the ability of the assessment among nursing students.Methods A set of standardized cases were designed based on an international standardized nursing language as well as a textbook of health assessment and reference.After validity of the set of standardized cases had been tested by the focus group and expert evaluation,the cases were applied to test the ability of the assessment among a total of 57 nursing students.Data were analyzed statistically.Results The average score of evaluation ability of nursing students was 42.5 (15～57).Nursing students got the highest score in psychosocial dimension,accounting for 50.7％ (13.93/27.5).Among five cases,the most difficult case was case 4,the degree of difficulty was 0.36.Conclusions This set of standardized cases can be used to assess the assessment ability among nursing students,and also can be used to evaluate the effects of training.

ObjectiveTo explore the factors influencing quality of life (QOL) in post-stroke patients.MethodsThe data of 76 first-stroke patients in three general hospitals at 1 month and 4 months after stroke were selected with Stroke Impact Scale and analyzed with multiple linear regression.ResultsThe influencing factors of patients' QOL at one months after stroke were education background, type of care, gender, pathological regions, marriage and complications. The education background was the most important influencing factor. The influencing factors of 4 months after stroke were rehabilitation nursing, marriage, social support and gender. Rehabilitation nursing was the most important influencing factor.ConclusionThe many factors influence QOL for post-stroke patients and are changed with time.

ObjectiveTo explore the factors influencing quality of life (QOL) in post-stroke patients.MethodsThe data of 76 first-stroke patients in three general hospitals at 1 month and 4 months after stroke were selected with Stroke Impact Scale and analyzed with multiple linear regression.ResultsThe influencing factors of patients' QOL at one months after stroke were education background, type of care, gender, pathological regions, marriage and complications. The education background was the most important influencing factor. The influencing factors of 4 months after stroke were rehabilitation nursing, marriage, social support and gender. Rehabilitation nursing was the most important influencing factor.ConclusionThe many factors influence QOL for post-stroke patients and are changed with time.

Implantable Neurostimulators ; Micro-Electrical-Mechanical Systems ; Microelectrodes

This review analyzed domestic and foreign literatures on intestinal absorption,introduced the current methods and models commonly used in phenolic glycosides researches and their impacts focused on intestinal absorption,summarized the research status,aims to provide a reference for improving their oral bioavailability,improve formulations,new medicine and clinical rational use of phenolic glycosides.

ObjectiveTo discuss the sonographic features of the urogential system in AIDS patients.MethodsThe positive sonographic lesions of urogenital systems of 336 AIDS patiens were analyzed retrospectively,with some compared with the results of aspiration biopsy,lab tests and clinical observation and treatment.ResultsThe ultrasonographic features of the pathological changes of the urogenital systems in AIDS patients were relative infectious diseases and relative tumors,which were respectively renal enlargement in 157 cases (46.73％),diffuse renal parenchymal disease in 129 cases (38.39％),urogential single infections in 125 cases (37.20％),and urogential cancer in 17 cases (5.06％).All of the above symptoms often developed in the same patient.ConclusionsThe ultrasonography of the lesions of the urogenital systems in AIDS patients are valuable documents for clinical diagnosis and treatment.

Porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) are very two important pathogens that have coursed huge economic losses in swine production in worldwide. In this study,a vector pCMV-TJM containing the full-length cDNA clone of PRRSV attenuated strain TJM-F92 was firstly constructed by PCR method. Then a gene sequence containing Afl II/Mlu I e restriction enzyme sites and a transcription regulatory sequence for ORF6 (TRS6) was inserted be- tween ORF7 and 3'UTR, yielding a expression vector pCMV-TJM-TRS. Subsequently, a plasmid pCMV-TJM-Cap was constructed by cloning of PCV2 ORF2 gene into the unique sites Afl II /Mlu I of pCMV- TJM-TRS plasmid DNA. Then three recombinant PRRSV, rTJM, rTJM/TRS and rTJM/Cap, were rescued by transfection of pCMV-TJM, pCMV-TJM-TRS and pCMV-TJM-Cap into Marc-145 cells, respectively,and confirmed by the genome sequence, restriction enzyme digestion, Western Blot and IFA. They all had the molecular markers which was different from the parent virus. The growth characteristics of the rescued viruses were similar to that of parent virus. rTJM/Cap could also express efficiently PCV2 Cap protein in Marc-145 cells. At passage 8, it still had PCV2 ORF2 gene which examined by RT-PCR. It indicated that the full-length cDNA clone of PRRSV attenuated strain TJM-F92 and recombinant PRRSV rTJM/Cap expressing PCV2 Cap protein were successfully constructed. It made an important foundation for studying on the pathogenic mechanisms of PRRSV and PRRSV-PCV2 vaccine in the future.
Animals ; Capsid Proteins ; genetics ; immunology ; Cell Line ; Circoviridae Infections ; veterinary ; virology ; Circovirus ; classification ; genetics ; metabolism ; Gene Expression ; Open Reading Frames ; Porcine Reproductive and Respiratory Syndrome ; virology ; Porcine respiratory and reproductive syndrome virus ; genetics ; metabolism ; Recombination, Genetic ; Swine ; Swine Diseases ; virology ; Viral Vaccines ; genetics ; immunology

ObjectiveTo investigate application value of parametric image processing in contrastenhanced ultrasound imaging in diagnosis of ovarian masses. MethodsFifty cases with ovarian masses underwent routine ultrasound and contrast-enhanced ultrasound imaging using a new dedicated parametric image processing software SonoLiver to analyze patterns of vascular formation and blood stream perfusion in the ovarian mass tissues on a digital video recorder in real time, compare their morphological characteristics of time-intensity curve (TIC) and dynamic vascular patterns (DVP) curve, and analyze quantitatively all indicators generated by SonoLiver.ResultsIn ultrasound imaging of the 50 cases, there were 24 cases (86％) displaying mainly blue lowly-enhanced imaging in those with benign masses and 15 cases (68％)displaying mainly red highly-enhanced imaging in those with malignant masses, with statistical significance (P ＜0. 01 ). There was significantly different characteristics of TIC and DVP between patients with benign and malignant masses. In 23 cases with benign masses, their DVP were significantly higher above the baseline than in those with malignant ones ( P = 0. 000), and in 15 cases with malignant lesions, their DVP were much shorter below the baseline than in those with benign ones, with statistical significance (P ＜0.05). The intensity of contrast medium, the time to reach its peak intensity and average transit time were all significantly higher in those with malignant masses than in those with benign ones (all P ＜ 0. 05 ). But, no statistical difference in the time of initial increasing between the two groups was found (P ＞ 0. 05). ConclusionsThere is significant difference in TIC and DVP of ultrasound imaging between benign and malignant masses, which if combined with contrast enhanced ultrasound parametric image processing can provide a more visualized quantitative information of benign and malignant ovarian masses with SonoLiver software.

Objective To investigate the nutritional status of elderly inpatients with benign orthopedic diseases and to assess its relationship with clinical outcomes.Methods Nutritional Risk Screening 2002 ( NRS 2002) was used to prospectively investigate undernutrition and nutritional risk in elderly patients hospitalized between April 1 and May 31, 2012 in Beijing Hospital for benign orthopedic diseases.Associations between nu-tritional status and clinical outcomes were analyzed.Results A total of 520 patients were included, with a mean age of (75 ±7.09) years.The mean body mass index (BMI) was (23.20 ±3.83) kg/m2, mean dominant-hand grip strength was (16.87 ±19.19) kg, mean mid-upper arm circumference was (25.62 ±3.81) cm, mean calf circumference was (31.92 ±4.02) cm.Compared with patients aged 65-79 years, patients≥80 years showed significantly lower hand grip strength [ (13.58 ±15.92) kg vs.(18.48 ±20.42) kg, P=0.004].All the pa-tients completed NRS 2002, which showed that 9.31%of the patients had undernutrition (BMI≤18.5 kg/m2), and 45.19%had nutritional risk (NRS 2002 score≥3).Compared with patients aged 65-79 years, patients≥80 years had significantly higher incidence of undernutrition (13.97% vs.7.21%, P=0.024) and nutritional risk (52.38%vs.41.76%, P=0.024), higher incidence of infectious complications in patients with nutritional risk (10.21%vs.5.26%, P=0.044), longer hospital stay [ (11.66 ±5.76) days vs.(10.42 ±4.37) days, P=0.016], and higher hospital expense [(20.28 ±1.811) thousand yuan vs.(16.39 ±1.362) thousand yuan, P=0.016].Conclusion Elderly patients hospitalized for benign orthopedic diseases have a high incidence of undernutrition and nutritional risk, which is associated with worse clinical outcomes.