Objective To analyze the distribution and drug sensitivity of pathogenic bacteria in urinary tract infection,and to provide evidences for rational use of antibiotics in clinic.Methods The data from 3 677 midstream urine specimens for bacterial culture were retrospectively analyzed.VITEK-2Compact was used to identify bacteria and test drag sensitivity.And WHONETS.6 was used to statistically analyze the data.Results Of 649 strains of pathogenic bacteria from urinary tract infection patients,there were 439 strains of gram-negative bacteria,accounted for 67.6％,among which there were 301 (46.4％) strains of Escherichia coli,and 74 (11.4％) strains of Klebsiella pneumoniae;there were 121 strains of gram-positive cocci,accounted for 18.7％,among which there were 97(14.9％) strains of Enterococcus,and 22 (3.4％) strains of Staphylococcus;there were 89 strains of fungi,accounted for 13.7％.According to drug susceptibility,the sensitive rates of Escherichia coli and Klebsiella pneumoniae to imipenem,cefotetan,piporacillin-tazobactam and amikacin were all over 87.0％.The drug sensitivity of Enterococcus to vancomycin and linezolid were both 100.0％.As to drug resistance to other drags,Enterococcus faecium was higher than Enterococcus faecalis.Conclusion Escherichia coli is the primary pathogenic bacteria of urinary tract infection.Every bacteria strain has a high drug resistance.In clinic,hospital should perform urine bacterial culture and drug sensitivity tests timely,and use antibiotics rationally in order to reduce the production and growth of drug-resistant bacteria.