Objective To study the influence of environment oxygen supplement and passive smoking on the blood gas indexes in rabbits.Methods Eighty adult Newzealand white rabbits were randomly divided into 4 groups,20 in each.The oxygen supplement and passive smoking group was exposed to cigarette smoke produced by 25 burning cigarettes,four hours per time,consecutively for twenty-four hours,simultaneously exposed to oxygen produced by two oxygen generators(600-1 000 ml/min per generator).The oxygen supplement group was exposed to oxygen produced by two oxygen generators(600-1 000 ml/min per generator) consecutively for twenty-four hours.The passive smoking group was exposed to cigarette smoke produced by 25 burning cigarettes,four huors per time,consecutively for twenty-four hours.The control group was exposed to general feeding environment.The analysis of variance of factorial design(2?2) was conducted,five blood gas indexes were measured.Results Under the condition of surroundings oxygen increasing,the partial pressure of blood oxygen was improved significantly(P0.05).No evident interaction was found between surroundings oxygen increasing and passive smoking.Conclusion Oxygen metabolism can be improved by environment oxygen supplement in rabbits,and the effect of passive smoking on the investigated five blood gas indexes is not considered significant.

Objective To investigate the effects of pravastatin on the expressions of hyperpolarization-activated cyclic nucleotide-gated channel subunits,HCN2 and HCN4 of right ventricular after myocardial infarction (MI) in rats. Methods Sprague-Dawley rats MI model was induced by ligating the left anterior descending coronary artery,and then 20 MI rats were randomly and equally divided into 24-hour group,1-week group,4-week group and pravastatin group (20 mg?kg-1?d-1 for 4 weeks by gavage). Another 5 rats served as sham-operation group. Real-time PCR and Western blotting were used to measure the mRNA and protein expressions of HCN2 and HCN4 in the heart tissues of each group. Results The mRNA and protein expressions of HCN2,and mRNA expression of HCN4 were up-regulated in 4-week group and pravastatin group compared with sham-operation group. The expression of HCN2 in pravastatin group were markedly attenuated compared with 4-week group. The protein expression of HCN4 was not detected in all groups. Conclusion The expression of HCN2 and HCN4 were up-regulated in myocardial infarction rats. Pravastatin may reverse the up-regulated expressions of HCN2 at mRNA and protein levels.

[Objective]To investigate the application value of the iliac allograft combined with anterior plate in the cervical anterior operation.[Method]Sixty-eight cases of anterior cervical decompression and fusion were performed with the iliac allograft combined with anterior plate.The data such as operative time,blood loss during operation and continue X-ray on their cervical spine were recorded,and were compared with the correspondent data of the control group which were transplanted of the iliac autograft combined with anterior plate.[Result]Compared with that of the control group(iliac autograft combined with anterior plate),the operative time and blood loss during operation in the group of iliac allograff combined with anterior plate was less(P

[Objective]To find a simple and direct feasible method to detect bone tissue vascularize from comparing advantage or disadvantage of ink intravascular Dabble-VG dyeing and other tissue dyeing in the tissue section capillary vessel display.[Methods]One side femoral artery of 6 normal goat was mixed with liquor composed of 10 percentink,10 percent formaldehyde and 20 percent mannitol,volume ratio in 7∶2∶1.Stopped femoral vein effluent was black.To get off goat tibia,10 percent formaldehyde was fixed with.EDTA decalcification,cedar oil douse,fiat 50 to 100 micrometer pachy-section and compound ink-VG dyeing to observe capillary vessel.[Results]Compound ink-VG dyeing ould display relations of capillary vessel and surrounding tissue.But in control group,single HE,Masson and Weigert Resorcinol-aniline red dyeing after-stain VG dyeing ould display few capillary vessel and surrounding tissue,and recognize difficultly.[Conclusion]Compound ink-VG dyeing can display capillary vessel of bone clearly,and can disclose relationships of surrounding tissue as well.This method can try on in tissue vascularization detect.

Objective To prepare and optimize fibrin-gel-coated vaneomycin alginate beads (FG-Vanco-AB)and investigate their possible use in treatment of osteomyelitis or prevention of infection.Methods Vancomycin alginate beads were produced by dropping vancomycin and alginate mixed liquor into calcium chloride solution.Beads including high vancomycin content were prepared and chosen by optimizing different concentrations of vancomycin solution and alginate solution.These beads were coated with fibrin gel formed by different concentrations of fibrin and the same concentration thrombin.The optimized beads were selected based on available release time,when vancomycin in medium could kill Staphylococcus aureus(ATCC25923). Results Higher content of vancomycin in bead resulted in increase of vancomycin concentration and alginate concentration in mixed liquid.The highest vancomycin content beads were prepared by 16%alginate and 50 mr/ml vancomycin,up to(27.36±0.90)%.The further results showed that vancomycin concentrations from beads coated with fibrin at 75 mg/ml and thrombin at 400 IU/ml could kill Staphylococcus aureus and remained above the breakpoint sensitivity for 19 days.Conclusion The available release time is prolonged,and the possibility of clinical use is conspicuously increased after vancomycin beads are optimized by adjusting the rate of mixed component and fibrin gel coat.

BACKGROUND: As reported in some literatures, articular dysfunction following limb lengthening is one of the commonest complications, but some scholars believe that the articular dysfunction after lower limb lengthening is avoidable providing appropriate technical operation and effective function prevention.OBJECTIVE: To investigate the changes of articular function of knee during limb lengthening in rabbits so as to provide a experimental basis for clinical tasks.DESIGN: A randomized controlled trial SETTING: Department of Orthopeadics, Affiliated Hospital of Some Military Medical University.PARTICIPANTS: Study was conducted in the Experimental Animal Center of the Third Military Medical University of Chinese PLA from February 2001 to June 2002. Totally 30 healthy adult New Zealand rabbits obtained from the Experimental Animal Center of the Third Military Medical University of Chinese PLA were randomly divided into lengthening group and delayed observation group(observed at 3 months after the stop of lengthening).METHODS: Every group was further divided into three sub-groups according to distraction range: 10%, 20% and 40%. Upper-tibia metaphysis distraction was adopted at the rate of 1 mm per day . Distraction and iconography changes of knee joint were observed with X-ray. The motive range of knee was investigated at the end of distraction and three months later.MAIN OUTCOME MEASURES: Articular motive range of the knee before the distraction, at the end of distraction and three months later, X-ray resutls RESULTS: ① There was no obvious radiographic change in knee at every time point in each group. ② Significant flexion disorder in knee was found in 20% distraction subgroup( P ＜ 0. 05), which was only 44. 7% of normal motive range in 40% distraction subgroup( P ＜ 0. 01) . Motive functions of knee in delayed observation group were significantly improved; thereinto,20% distraction subgroup returned to preoperative level, while 40% distraction subgroup also had significant improvement compared with that of 3 months earlier but still lower than normal level, which was only about 88.5% of normal level( P ＜ 0.05).CONCLUSION: Temporary articular dysfunction might occur during limb lengthening process, and mostly during big range of distraction. Although the dysfunction could be gradually recovered after the stop of lengthening, active prevention is still necessary.

Objective To evaluate the stability and compressive mechanical functions of the lumbar spine following insertion of a new self-made allograft interbody fusion cage.Methods Anti-bending intensity with three points test,anti-rotation intensity and compressive stiffness were measured at L4-L5 lumbar spine on five adult human fresh cadaveric specimens following insertion of a new self-made allograft interbody fusion cage and compared with that of before and after nucleus pulposus removal.Results The anti-bending intensity of flexion and extension of lumbar spine after inserting a new allograft interbody fusion cage was increased significantly(P

Objective To explore the effectiveness of monitoring for developmental delay (DD) and the feasibility of firsthand data reporting among 0~3 years old children. Methods A study population consisted of a cluster sample of 20907 children aged 0~3 years, from 22 communities in 5 districts in Beijing, was monitored for 1 year using questionnaires and data reports. The results were compared against the non-project communities. Results The screening rate of DD was 62.18%, higher than the non-project groups, and the rate of 0 year old group was significantly higher than the other groups. 268 children were positive screening, referral of 253 children, the referral rate was 94.4%; 97 children were diagnosed as DD with the prevalence rate of 7.46 ‰; 43.28% of the children received intervention and rehabilitation training. Conclusion The surveillance means of DD for 0~3-year-old children are effective and the firsthand data report is feasible

OBJECTIVE To investigate the clinical value of human leukocyte antigen DR(HLA-DR) expression on monocytes in prediction of prognosis in patients with hospital acquired pneumonia(HAP).METHODS A total of 68 patients suffered from HAP and transferred to intensive care unit(ICU) were enrolled in the study and divided into survivors group and nonsurvivors group according to prognosis.HLA-DR expression on monocytes,acute physiology,age and chronic health evaluation((APACHE)) Ⅱ score,serum cortisol level,T lymphocytes(subpopulation) and natural killer(NK) cell were observed.Relationship between HLA-DR expression on(monocytes) and APACHE Ⅱ score and 28-day mortality rates was analyzed through Logistic regression model.(RESULTS) HLA-DR expression on monocytes in nonsurvivors was significantly lower than that in survivors (P

The evaluation of undergraduate course teaching by the Ministry of Education is a challenge to all our teachers,but it is also a chance for us to improve the teaching quality of infectious disease class.In this article we summarized the experience of the infectious disease education reform of many years,and discussed the approach and means of improving the teaching quality of infectious disease class.