Objective To establish a method for the determination of evodiamine and rutaecarpine of compound wuzhuyu ointment.Methods HPLC method was used with Agilent HC-C18(250 mm× 4.6 mm,5 μm) column and the mobile phase being acetonitrile-0.08％ ocane sutfonic acid sodium(41:59).The flow rate was 0.8 ml/min,the column temperature was 30℃,and the UV detector was set at 225 nm.Results The linear ranges of evodiamine and rutaecarpine were 0.62～ 12.40 μg/ml (r=0.9997) and 0.24～4.8 0 μg/ml (r=0.9996),respectively.The average recovery of evodiamine and rutaecarpine was 97.9％ and 98.0％,respectively.Conclusion The method is simple,repeatable and accurate.It can be applied in quantitative determination of evodiamine and rutaecarpine in compound Wuzhuyu ointment.

Objective To establish a method for the determination of borneol, isoborneol, menthol in Bingshuang-Meisu pill.Methods The capillary column PEG-20M(30 m × 0.32 mm, 0.25μm) was used, naphthalene was adopted as internal standard substances, split injection(split ratio 10:1), temperature programme, the injector temperature was 240℃, the detector temperature was 250℃.Results The linear response range of menthol, borneol, isoborneol were 13.00-104.00μg/ml, 6.80-54.40μg/ml, 5.40-43.20μg/ml and the average recovery were 97.90%, 98.05% and 97.77%, RSD were 0.98%, 1.53% and 1.40% respectively. Conclusion The method was highly sensitive, accurate, and repeatable. It can be an important method to control the standard ofBingshuang-Meisu pill.

Objective To investigate the therapeutical effect of Tetrandrine(Tet)combined with Glutathione(GSH)in rats with Parkinson's disease(PD).Methods The PD model rats were established through stereotactic microinjection of 6-hydroxydopamine,then they were randomly divided into PD group,GSH group,L-dopa group,GSH+Tet+L-dopa group and GSH+Tet group.The rats were peritoneal injected with corresponding medicines.The circling behavior of rats were observed induced by Apomorphine.The levels of GSH in substantia nigra and MAO-B in striatum were assayed after treatment,the numbers of dopaminergic neuron were observed by immunohistochemistry and the contents of TH mRNA were also be assayed in each group.Results(1)Compared with pretherapy,the rotation times of groups L-dopa,GSH +Tet+L-dopa,GSH+Tet had statistical significance(P

Objective To provide a basis for further exploration of Dunaliella Salina by determining the main active components in the extract of Dunaliella salina. Methods The carotenoids and vitamins were determined by HPLC or colorimetric method, amino acids were analyzed by amino acid analyze. Unsaturated fatty acid was determined by GC. Atomic absorption spectrophotometry was used to detect K, Na ,Ca, Mg, Cu, Zn, P, Fe, Mn and SPF was used to detect Se. Results There were affluent carotenoids, vitamins, amino acids, unsaturated fatty acid and trace elements in the extract of Dunaliella salina. Conclusion The extract of Dunaliella salina could be used as natural raw material to develop the supplement food.

Objective To evaluate the specificity of Architect chemiluminescence immunoassay ( CLIA) for diagnosis of syphilis in the clinical screening test.Methods 65 774 syphilis specific antibody results in the Beijing Tongren Hospital were retrospectively analyzed.In this study, Architect CLIA for diagnosis of syphilis was used as a screening test for the pre-operative patients from August 2011 to July 2012.All the repeatedly reactive samples were tested by the tolulized red unheated serum test ( TRUST) and Treponema pallidum particle agglutination ( TPPA) assay.Samples with with discordant results were verified by western blot ( WB) Finally, all the results were statistically analyzed by software SPSS 17.0.Results Among 65 774 samples, 940 (1.43%) were found to be repeatedly reactive using the Architect CLIA.Of these sera, 330 ( 35.11%) were reactive and 610 ( 64.89%) were nonreactive by TRUST.Because a TRUST titer of 1:1was not always sufficient to confirm a TPPA reactive result, all the samples with CLIA reactive results were finally performed by TPPA assays.The results showed that 843 ( 89.68%) were positive and 97 (10.32%) were negative.After arbitrated by the WB, out of 97 TPPA negative sera, 18 (18.56%) were positive;7(7.22%) were indeterminate and 72(74.23%) were negative.Conclusions The specificity of Architect CLIA for diagnosis of syphilis was 99.89%.The results showed that confirmation by TPPA on sera with screening test for syphilis by Architect CLIA effectively decreased the false-positive results and could be suitable for a routine supplementary for syphilis.However, if necessary, the WB should be further utilized.

Purpose　Both the working standard and corresponding sample(GRAN75)were first calibrated by WHO international standard for G-CSF.Methods　MTT method by NFS-60 cells was used. The results were calculated by (4,4)method.Results　Three batchs working standards were prepared,two batchs were freeze-dry and the prescription was same as WHO international standard for G-CSF,one batch was injection and the prescription was same as corresponding sample(GRAN75).The biological potency and the FL% of average potency were 3.062×106 、4.276×106IU/ampoule、1.635×107IU/ml and 5.529%、4.291%、4.244% for working standard, and 1.880×107IU/ml and 5.175% for corresponding sample,respectively.Conclusion　The working standard which calibrated could be used as working standard in the measurement of rhG-CSF biological activity.

Purpose　The determination method of rhG-CSF bio logical activity by NFS-60 cells was studied in accordance with WHO internation al standard for G-CSF.Methods　MTT method was adopted.R esults　The chomosone number of NFS-60 cell was 39, about 1×105 cells/ml to 1×107 cells/ml,the dying color showed gradient when the NFS-60 c ells was dyed by MTT,the method(4,4) was adopted in the determination of rhG-CS F biological activity,the average FL% of potency was 13.560% for single exprimen t,and the CV of inter-assay and intra-assay was lower than 10% and 10.109%,respectively.Conclusion　method(4,4) can be us ed in the determination of rhG-CSF biological activity,and the results can guid e the study and the manufacture of rhG-CSF effectively.

Purpose To analyze the relationships between glomerular filtration rate (GFR) measured by SPECT and renal parenchyma thickness and enhanced CT value measured by CT, and to explore predictive significance of CT in evaluating renal function of patients with hydronephrosis.Materials and Methods One hundred and fifteen patients diagnosed with unilateral hydronephrosis by ultrasound were retrospectively analyzed. GFR% (GFR percentage of affected kidney to the both kidneys) was measured by SPECT. CT% (percentage of affected renal parenchyma thickness to the both kidneys) and enhanced CT% (percentage of enhanced CT value of affected kidney to the both kidneys) were measured by pre- and post-contrast CT scan. According to GFR, the renal function was divided into mild-to-moderate impairment, severely impairment and non-function. Twenty-five volunteers were recruited as control group. CT%, enhanced CT% and GFR%among the four groups were compared, and the correlation of CT% and enhanced CT%with GFR% was analyzed to evaluated CT in predicting renal function.Results CT%, enhanced CT% and GFR% in mild-to-moderate impairment group was significantly greater than those in severely impairment group and non function group (F=20.24, 7.78 and 329.21,P<0.05). GFR% was positive correlated with CT% (r=0.58,P<0.05) and enhanced CT% (r=0.61,P<0.05). Area under curve (AUC) of CT% were 0.54, 0.79 and 0.83 for mild-to-moderate impairment, severely impairment and non-function, with sensitivity of 92.91%, 93.47%, 65.72%, and specificity of 35.33%, 59.47%, and 88.62%, respectively. AUC of enhanced CT% were 0.79, 0.89 and 0.96 for the three groups, with sensitivity of 97.51%, 80.02%, 97.66%, and specificity of 58.14%, 89.82% and 94.27%, respectively.Conclusion There was high correlation between renal function imaging by SPECT and CT in evaluating renal function of hydroneohrosis patients. Pre- and post-contrast CT scan can be used as complements in predicting renal function, and post-contrast CT with high accuracy.

Objective To understand the status and difference of breastfeeding behavior in urban and rural areas of Southwest China.Methods From March to July in 2011,3659 infants aged 6-24 months were selected by stratified cluster randomized sampling method in urban and rural areas of three provinces of Southwest China (Sichuan,Yunnan and Guizhou),including 1801 (49.2％)infants from urban areas and 1858(50.8％) from rural areas.Basic information of these infants,their families and breastfeeding was obtained by a questionnaire for the mothers or baby-carers.Descriptive analysis and survival analysis (Kaplan-Meier method) were used to describe breastfeeding behavior.Chi-square test and Log-Rank test were used to identify the differences of breastfeeding behavior between urban and rural areas.Results Early breastfeeding initiation rate within one hour after birth was 10.7％(355/3315),and the numbers of urban and rural areas were 12.3％(198/1604) and 9.2％ (157/1711),respectively,x2 =8.691,P＜0.05.Totally,20.3％(725/3575) of all infants were initially fed by breast milk after delivery,and 25.1％ (440/1754) in urban areas and 15.7 ％ (285/1821) in rural areas,x2 =49.192,P＜0.05.The exclusive breastfeeding rate and breastfeeding rate within four months after birth were 35.5％ (27.4％ in urban and 43.6％ in rural areas,x2=88.678,P＜0.05)and 76.2％ (68.5％ in urban and 84.3％ in rural areas,x2 =124.702,P＜0.05),respectively.However,the exclusive breastfeeding rate and breastfeeding rate within six months after birth reduced to 11.3％ (7.9％ in urban and 14.4％ in rural areas,x22 =18.001,P＜0.05) and 65.0％ (54.0％ in urban and 76.3％ in rural areas,x2 =199.662,P＜0.05),respectively.The median breastfeeding duration was 8.0 months (7.0 months in urban and 9.0 months in rural areas,x2 =96.780,P＜ 0.05).The most common reason of weaning was insufficient breast milk which accounting for 48.7 ％ of families [56.9％ (1161/2385) in urban and 39.9％(462/1157) in rural areas,x2=68.840,P＜0.05].Conclusions In Southwest China,intervention program should be implemented to improve the breastfeeding status.Breastfeeding behaviors are different between urban and rural areas in Southwest China.The initiation of breastfeeding in urban area is better,but the sustainability of breastfeeding is better in rural area.

Objective To investigate the relationship between serum uric acid (UA) level and abdominal obesity or metabolic syndrome (MS).Methods A total of 875 subjects,with 350 males and 525 females,aged 40-65 years old,were enrolled in this study.The clinical and biochemical data were collected and MRI was used to assess the visceral and subcutaneous adipose tissues.The relationships between UA level and abdominal obesity or MS were analyzed,and the cut-off values of UA for abdominal obesity and MS were determined.Results Raised risks of abdominal obesity (OR =4.35,95％ CI 1.91-9.90 in males; OR =5.44,95％ CI 2.41-12.31 in females) and MS (OR =4.47,95 ％ CI 2.08-9.62 in males; OR =11.62,95％ CI 3.43-39.37 in females) were observed with the increase of UA level.The multiple logistic regression analysis showed that UA was an independent risk factor for hypertriglyceridemia (OR =2.23,95％ CI 1.02-4.87 in males ; OR =3.04,95％ CI 1.49-6.23 in females) in all subjects and for abdominal obesity(OR =3.23,95％ CI 1.32-7.91) and hypertension (OR =2.35,95％ CI 1.37-4.05)in the females.Among the females,the regression line analyzed by simple correlation indicated that the UA level of 244.0 μmol/L was corresponded to the visceral adipose tissue area of 80 cm2.The optimal cut-off point of UA for the diagnosis of MS was 258.8 μmol/L determined by the receiver operating characteristic curve.Conclusions The level of UA is closely correlated with abdominal obesity and MS in the middleaged Chinese.The elevated UA level is an independent risk factor for abdominal obesity and MS in the female.