Objective : To explore the impact of high glucose ( HG) intervention on immune escape of pancreatic cancer cells and its molecular mechanisms． Methods : PANC-1 cells were treated with different concentrations of glucose (0，7. 5，15，30 mmol / L) for 24 h to establish high glucose intervention PANC-1 cells．miR-429 mimics and its negative control ( mimics NC) were transfected into PANC-1 cells，which were divided into control group，HG group，HG + mimics NC group，HG + mimics group，HG + mimics + oe-NC group，and HG + mimics + oe-ZEB1 group．Flow cytometry was utilized to measure the expression level of cell surface molecule PD-L1 ; qRT-PCR was used to detect the expression levels of miR-429 and ZEB1 mRNA in cells ; Western blot was used to detect the ex- pression level of ZEB1 protein in cells．The above-mentioned PANC-1 cells from each group were co-cultured with CD8 + T cells to establish a co-culture system，and CCK-8 was used to assess cell proliferation activity ; apoptosis levels of cells were measured using flow cytometry ; lactate dehydrogenase ( LDH) release assay was used to detect the killing effect of CD8 + T cells on PANC-1 cells ; dual-luciferase reporter system was used to validate the target- regulatory relationship between miR-429 and ZEB1 . Results : HG could promote the expression of cell surface mole- cules PD-L1 and ZEB1 in PANC-1 cells (P＜0. 05) ，inhibit the expression of miR-429，and exhibit concentration dependence．Overexpression of miR-429 could significantly suppress the expression of cell surface molecule PD-L1 induced by HG in PANC-1 cells，while overexpression of ZEB1 could reverse the inhibitory effect of miR-429 over- expression on the expression of cell surface molecule PD-L1 induced by HG．After establishing a co-culture system with CD8 + T cells，compared with the control group，the proliferation activity of PANC-1 cells in the HG group sig- nificantly increased，and the apoptosis rate and cytotoxicity significantly decreased (P＜0. 05) ．Compared with the HG + mimics NC group，the proliferation activity of PANC-1 cells in the HG + mimics group significantly decreased， and the apoptosis level and cytotoxicity significantly increased (P＜0. 05) ．Compared with the HG + mimics group， the proliferation activity of PANC-1 cells in the HG + mimics + oe-ZEB1 group significantly increased，and the apop- tosis rate and cytotoxicity significantly decreased (P ＜0. 05 ) ．Dual luciferase reporter gene assay confirmed that miR-429 negatively regulated ZEB1． Conclusion High glucose promotes immune escape of PANC-1 cells by down- regulating the expression level of miR-429，negatively regulating the expression of ZEB1 mRNA，and increasing the expression level of cell surface molecule PD-L1 in PANC-1 cells.

Active medical device is a kind of medical device which is widely used. In order to realize the goal of high-quality development, product with high reliability is a necessary requirement for the domestic active medical device industry. By means of literature research, data collection, field research, materials comprehensive combing and analysis, this paper systematically analyzes and studies the current situations and the existing problems of reliability and evaluation from the dimensions of Chinese active medical device industry policy, enterprise situation and evaluation method. In addition, by considering the technical characteristics of reliability work, concrete suggestions for solving the problems are given from the directions of standard and guiding principle, so as to provide reference for active medical device industry to develop scientific and objective reliability technical standard system and guiding principle, which are in accord with the current characteristics of Chinese active medical device industry and supervision.
Reproducibility of Results ; Industry ; China