Objective To investigate the effects of LncRNA MT1JP on the migration and invasion of uveal melanoma cell,and explore its regulatory mechanism.Methods The expression level of LncRNA MT1JP in uveal melanoma cell line and normal retinal pigment epithelium cell line were measured by qRT-PCR.The SP6.5 cell line was divided into three groups,MT1JP group transfect with pcDNA3.1-MT1JP,siMT1JP group transfect with pcDNA3.1-siMT1JP and NC group transfect with negative pcDNA3.1 plasmid.The migration and invasion ability were measured by cell wound scratch assay and transwell assay.Western blot was used to measure the expression level of P53 protein.Results The expression level of LncRNA MT1JP in SP6.5 and M23 cell line was 0.20 ± 0.02 and 0.31 ± 0.01,respectively,which was significantly lower than 1.0 in ARPE-19 cell line (all P ＜0.01).The wound healing rate in siMT1JP group was 61.50 ± 3.70％.while,NC group was 20.0％ ± 2.10％,and MT1JP group was 10.6％ ± 1.7％,there was no statistical difference among three groups (all P ＜ 0.01).The invasion cell number was 75.6 ±6.8 in siMT1JP group,which was significantly more than 29.5 ± 3.9 in NC group,and 10.3 ±2.6 in MT1JP group,there was no statistical difference among three groups (all P ＜ 0.01).The expression level of P53 protein in siMT1JP group was 0.41 ± 0.04,which was significantly lower than 1.0 in NC group (P ＜ 0.01).However,the expression level of P53 protein in MT1JP group was 5.73 ±0.62,which was significantiy higher than 1.0 in NC group (P ＜ 0.01).Conclusion LncRNA MT1JP inhibits the migration and invasion of uveal melanoma cell,which may be associated with upregulation of P53 expression.

Objective To examine the effects ofulinastatin (UTI) on cardiac dysfunction in mice with heat stroke and its possible mechanism.Methods 20 mice were divided into four groups randomly:room temperature plus normal saline (Sham+NS),room temperature plus UTI (Sham+UTI),heat stress plus normal saline (HS+NS),heat stress plus UTI (HS+UTI),5 each.105U/kg UTI was delivered by intraperitoneal injection before the onset of the heat stress.Room temperature groups were housed at room temperature (23.0 ± 0.5 ℃),while heat stress groups were kept in an incubator at 36.5 ± 0.5 ℃ and humidity of 65.0％ ± 2.0％.The rectal temperature (Tr) reaching 42℃ was taken as severe heat stroke,and the time in two heat stress groups was recorded.The mice were transferred to the room temperature (23.0 ± 0.5 ℃) for natural cooling after the heat stroke onset.6 hours after the treatment,cardiac output (CO) was ultrasonographically detected,the myocardium was separated for histopathological examination and the expression of total p38 and phosphorylated p38 (p-p38) was determined by Western blotting.Results The time to reach 42℃ in HS+UTI group was significantly prolonged (P=0.044).Compared with the Sham+NS group,the CO in HS+NS and HS+UTI group decreased significantly (P=0.017),and the score of myocardial inflammation (P＜0.001) and p-p38/p38 ratio (P＜0.001) increased.The CO was significantly higher in HS+UTI group than in HS+NS group (P=0.030),and the score of myocardial inflammation (P＜0.001) and p-p38/p38 ratio (P=0.001) were significantly lower.Conclusion Ulinastatin might improve the cardiac function in mice with heat stroke by decreasing the p-p38 and alleviating the inflammation response.

Objective To investigate the effect of ultrasound-guided bilateral superior laryngeal nerve internal branch block combined with endotracheal surface anesthesia in patients with hypertensive cerebral hemorrhage for the duration of postoperative tube insertion.Methods A total of 100 emergency hypertensive intracerebral hemor-rhage surgical patients who visited our hospital from October 2021 to April 2023 were included as the research subjects.They were randomly divided into four groups,25 patients in each group.After surgery,group U received bilateral superior laryngeal nerve internal branch block under guidance of ultrasound combined with endotracheal surface anesthesia,group C1 received bilateral superior laryngeal nerve internal branch block under guidance of ultrasound,group C2 received endotracheal surface anesthesia,and group C3 did not undergo any procedure after surgery.Hemodynamic changes(HR,MAP,and SpO2)at different time points during the postoperative tube insertion in four groups of patients were recorded.The frequency of restlessness within 10 hours after surgery,the dosage of dexmedetomidine and urapidil,the volume of wound drainage,and the satisfaction of bed nurses were also recorded.Results There was no statistically significant difference in general conditions among the four groups(P>0.05).There was no statistically significant difference in HR and MAP at different time points in Group U(P>0.05),while the differences among the other three groups were statistically significant(P<0.05).At the same time point,the MAP and HR of group U and C1 were significantly lower than those of group C3(P<0.05),and the MAP of group U was significantly lower than that of group C2(P<0.05).There was no statistical difference in SPO2 among the four groups of patients at the same time point(P>0.05);The frequency of restlessness,dosage of dexmedetomidine and urapidil,and volume of wound drainage in the U and C1 groups were significantly lower than those in the C2 and C3 groups(P<0.05).Except for the difference in restlessness frequency(P<0.05),there was no statistical difference in other indicators between group U and C1;There was a statistical difference in satisfaction among the four groups of nurses(P<0.05,C3>C2>C1>U group).No nerve block related complications were observed in the U and C1 group.Conclusion Ultrasound-guided bilateral superior laryngeal nerve branch block combined with endotracheal surface anesthesia can maintain hemodynamics steadily of the postoperative patients in the NICU to varying degrees and reduce the frequency of postoperative restlessness,the dosage of sedative and anti-hypertensive drugs,while reducing the flow of wound drainage,and improve the satisfaction of bed nurses.