Objective:To analyse the therapic effect of low-voltage electrochemotherapy (EP) on the head and neck cancer.Method:YCU-N861 human head and neck cancer cells were subcutaneously inoculated in the neck of nude mice to establish tumor-bearing models. Then the mice were treated with bleomycin(BLM, 20 μg per mouse), accompanied with electric stimulation(50 V or 100 V).Result:BLM treatment applied with EP can significantly inhibite the proliferation of tumor cells than BLM or EP treatment seperately(P<0.01) . The tumor volume, the mitotic index (MI) and the labeling index (LI) of Ki67 in BLM+EP group were all obviously decreased than BLM group, EP group and control group (P<0.01).Conclusion:Low-voltage electrochemotherapy can offer promising mothod in the local treatment of head and neck cancer.

we reviewed data from 547EEGs on 400 patients with epilepsy in order to determine the probability in finding the interictal epileptiform activity (IIEA) with serial EEGs. In 49.5% of our patients, the abnormaeity is present on the first record. In 53.5% by the second EEG, and in 54.25% by the third or above. There is relatively little yield to serial EEGs beyond this point. Patient's ages, types of seizures and causes are negatively correlated to IIEA yield

The genetic diagnosis of hemophilia is a basic method for preventing the birth of hemophilia patients and a basic way for realizing the aim of aristogenesis.Direct and indirect genetic diagnosis is a basic strategy for screening of hemophilia;while in indirect genetic diagnosis,more attention should be paid on the information given by the intro-genetic sites combined with the extra-genetic sites.

Objective To evaluate the validity, reliability and feasibility of Traditional Chinese Medicine Scale for Children with Cerebral Palsy. Methods34 children with cerebral palsy and 34 healthy children were assessed with the Traditional Chinese Medicine Scale for Children with Cerebral Palsy. The split-half reliability, Cronbach's α coefficient, content reliability, difference validity and feasibility were tested. ResultsSplit-half reliability of this scale was 0.6199, Cronbach's α>0.7. The scores of this scale could differentiate the cerebral palsy from health children. The acceptance rate and the completion rate were both 100%. The median of the complete time was 10～15 minutes. ConclusionThe Traditional Chinese Medicine Scale for Children with Cerebral Palsy is a tool to evaluate cerebral palsy with satisfactory reliability, validity and feasibility.

OBJECTIVE: This research aims to investaigate the effect of cetuximab and dendritic cells (DCs) to kill the head and neck squamous cell (HNSCC), in order to provide a new way for the patients of HNSCC. METHOD: DCs were induced from peripheral blood monocytes by rhIL-4, rhGM-CSF and TNF-alpha in vitro, 7days later, detecting the surface marks of DCs for example CD83, CD86, and then using MTT and flow cytometry detecting the effect T lymphocytes induced by DCs combining cetuximab to kill HNSCC; EGFR and pEGFR in each group were anlysised by Western blot. RESULT: It is successful to induce DCs in vitro. Mature DCs (mDCs) expressed the suface mark such as CD83, CD86 higher compared with immature DCs (imDCs). Compared with other groups, cetuximab combined with DCs significantly enhanced the cytotoxicty and apoptosis to HNSCC (P < 0.05). pEGFR were gradually reduced as the concenetration of cetuximab increasing (P < 0.05). However, comparing with the group of cetuximab, the group of cetuximab combined with DC has no significant difference at the same concentration of cetuximab. In each group EGFR also has no significant diference (P > 0.05). CONCLUSION Cetuximab and DCs have synergistic effects, which can significantly enhance the killing effect of HNSCC.
Antibodies, Monoclonal, Humanized ; pharmacology ; Apoptosis ; drug effects ; Carcinoma, Squamous Cell ; pathology ; Cetuximab ; Dendritic Cells ; immunology ; Head and Neck Neoplasms ; pathology ; Humans ; Squamous Cell Carcinoma of Head and Neck ; Tumor Cells, Cultured

Neurology is a professional science. The content of neurotomia, sub-speciality knowl-edge and auxiliary examination are abundant, and meanwhile it is rather difficult. Clinical graduates after graduation are malnly engaged in clinical work, clinical services, and will play a variety of roles in the course of their clinical practice so it is necessary for them to learn comprehensive knowledge. This article introduced experience of clinical postgraduates tralning of neurology, such as doctor-patient communication skills, basic theoretical study (neurology, neurotomia and sub-speciality knowledge), clinical thinking, and assistant examination, clinical research capacity during these 18month. It will provide the theoretical and practical knowledge in the future work.

Objective To investigate the curative effects of partial internal sphincterotomy in the posterior midline and lateral position in treatment of chronic anal fissure. Methods One hundred and fifty cases chronic anal fissure patients with stageⅡ or Ⅲ were randomly divided into observation group(80 cases)and control group(70 cases),who were hospitalized in the Shunyi District Hospital of Beijing from Dec. 2009 to Oct. 2012. The patients of two group were treatment by internal sphincterotomy either in the posterior midline or lateral position respectively. The efficacy,healing time,complications and recurrence of the two groups were recorded and compared. Results There were 76 cases with cure,6 cases with improvement and 2 with ineffective effect in the observer group. There were 49 cases with cure,12 cased with improvement and 9 cases with ineffective effect in control group. The difference of the efficacy was statistically significant( P =0. 025). Wound healing time of observation group and control group were(9. 9 ± 0. 8)d and(17. 1 ± 0. 9)d,and the difference was significant( P =0. 048 ). The incidence of anal exudates in observer group was lower than the control group(2. 5%(2/80)vs. 24. 3%(17/70);P=0. 032). The incidence of recurrence in observer group was 5. 0%(4/80),lower than the control group(24. 3%(17/70),P=0. 046). The degree of the postoperative pain,voiding dysfunction and wound edema in observer group were lower than the other group( P =0. 025, 0. 025,0. 049). Conclusion The effect of internal sphincterotomy in the lateral position in treatment of chronic anal fissure is better with quickly repair,fewer complications.

Objective To evaluate the inhibitory effect on the VX2 tumor of rabbit with CT-guided percutaneous high-temperature pingyangmycin lipiodol injecting. Methods New Zealand white rabbits were performed to plant rabbit VX2 tumor in the left liver leaf. After two weeks, all the rabbits were scanned with CT and divided into 5 groups randomly, and every group was injected different temperature pingyangmycin combined with lipiodol, then the effect was evaluated after 2 weeks. Results Compared with control group, the volume of VX2 tumor in every group injected high-temperature lipiodol reduced obviously (P<0.05), and the necrosis rate of hepatic transplant tumor increased significantly (P<0.05) as the temperature increased. MVD value of the tumor and VEGF expression obviously reduced (P<0.05), there was a positive relationship between MVD value and VEGF expression with the temperature. Conclusion CT-guided percutaneous high-temperature pingyangmycin lipiodol injecting can inhibit the VX2 tumor growth and tumor angiogenesis formation obviously, and the higher temperature, the better the therapeutic effect.

Objective To clone the full-length cDNA of rat B cell lymphoma-2(bcl-2) gene,then construct and identify the cytomegavirus-mediated lentiviral expression vector of bcl-2 gene,and assess the gene expression in 293T cell,which is a human embryonic kidney cell line.Methods The full-length bcl-2-cDNA fragment was amplified by reverse transcription polymerase chain reaction(RT-PCR) from the kidney tissue of a Wistar rat.The double-stranded oligonucleotides(dsOligoe) were then cloned into the pMD18-T plasmid.After confirmation of a correct construction by sequencing,the positive clone was subcloned into pGC-FU vector with enhanced green fluorescent protein(EGFP),and then transformed into DH5a competent cells.The restricted endonuclease and T4 DNA ligase were used to construct the lentiviral expression vector plasmid pGC-FU-bcl-2 which,combined with the lentiviral packing materials(pHelper 1.0,pHelper 2.0),was then transfected into 293T cell line to form the recombinant lentivirus pGC-FU-bcl-2,and it was used to transfect the 293T cells.The expression of pGC-FU-bcl-2 was further verified by detecting EGFP and bcl-2.Results 1) It was verified by DNA sequencing that the sequence of rat bcl-2 gene was consistent with reported sequence in GenBank.2) The bcl-2 gene was successfully combined in pGC-FU-bcl-2 recombinant plasmid which could be transfected into human embryonic kidney cells.3) The recombinant virus pGC-FU-bcl-2 could be obtained from the 293T cells by co-transfection of pGC-FU-bcl-2 and packing plasmids.4) Targeting gene could be cloned into 293T cells by the recombinant lentivirus with steady expression.The fluorescent protein could be observed under microscope and the expression of bcl-2 protein was detected by Western blotting.Conclusions The lentiviral expression vector containing EGFP and bcl-2 gene has been successfully constructed,with which the transfected 293 T cells can lead to a steady expression of bcl-2 protein.The present study provides a basis for the further study of the function of bcl-2 gene and a potential therapy for the diseases relating to apoptosis.

Objective To analyze the curative effect of double path endoscopic surgery in the treatment of fungal sinusitis,and to compare with the effect of simple in endoscopic nasal passages windowing.Methods The clinical data of fungal sinusitis patients were analyzed.According to the different surgery way,the patients were divid-ed into two groups.The observation group:nasal endoscopic nasal passages open a window in the joint of fangs nest small windows double path;Control group:simple in nasal endoscopic fenestration.Results The observation group had 35 cases and the control group had 37 cases.After treatment,the symptoms score of the observation group was sig-nificantly lower than the control group [(1 .09 ±0.83)points vs (1 .47 ±0.77)points;t =2.01 5,P =0.048].The effective rate of the observation group was significantly higher than the control group (94.29% vs 75.68%;χ2 =4.81 3,P =0.028).7 days after treatment,the C -reactive protein(t =4.971 ,P <0.01 ),TNF alpha(t =9.1 9,P <0.001 ),beta IL -1 (t =5.635,P <0.01 ),white blood cells (t =3.25,P =0.002)and IL -6(t =3.86,P =0.002) in the observation group were significantly lower than the control group (P <0.05).The preoperative plasma level of 1 -3 -β-glucan D between the two groups had no statistical difference (P >0.05).2(t =5.978,P <0.01 ),4(t =6.335,P <0.01 ),6(t =9.348,P <0.01 ),and 8 days(t =8.226,P <0.01 )postoperatively,1 -3 -β-glucan D levels were significantly lower than the control group,the differences were statistically significant (P <0.05).The postoperative recurrence rate of the observation group was significantly lower than the control group (8.57% vs 27.03%,the Log -rank χ2 =4.061 ,P =0.044).Conclusion In fungal sinusitis treatment,the use of endoscopic nasal passages open window joint fangs nest small windows double path can more effectively remove lesions,its forward curative effect is superior to pure in nasal endoscopic fenestration.