Objective To explore the application of LEEP in treatment of CIN and to identify the risk factors for residual lesions and recurrence of CIN after treatment of LEEP.Methods 253 patients with CIN were given treatment of LEEP.Cure rate,recurrence and residual lesions rate were observed.Pathologic examination results before and after treatment of LEEP were compared,risk factors for residual lesions and recurrence of CIN after treatment of LEEP were identified from age,generation,TCT examination,HPV-DNA,pathologic examination and vertebral cutting edge of specimens,et al.Results LEEP was featured with characteristics of fast,minimal trauma and low incidence of complications,cure rate was 80.24％,residual lesions and recurrence rates were 11.46％ and 8.30％,diagnose accordance rate before and after LEEP was 78.26％,positive examination of vertebral cutting edge of specimens was the risk factor for residual lesions and recurrence of CIN after treatment of LEEP.Conclusion LEEP is an ideal methods for diagnosis and treatnent of CIN,follow-up and retreatment should be applied for CIN patients with positive examination of vertebral cutting edge of specimens after treatment of LEEP.

To improve the curative effect in chronic hepatitis B is a major problem to be solved. With the development of technology, an increasing number of novel markers have been used to guide treatment selection, to predict the curative effect and prognosis of hepatitis B.This paper focuses on the characteristics, applications and developmental trend of serological, virological and immunological markers associated with curative effect of chronic hepatitis B.

Statistical analysis of TCM syndrome differentiation and clinical symptoms were made in 163 cases of diseases of digestive tract. It was found that the patients of abnormal defection or tendecy of abnormal defecation with the hyperactive liver - Qi attacking the spleen, stagnation of liver-Qi due to hypofunction of the spleen, loose stool due to deficiency of the spleen, constipation due to Qi-deficiency, constipation due to Qi - staganation syndromes accounted for 60%- 100%. Elecrtic examination of colon on the body surface and analysis of frequancy spectrum found that hyperelectroactivity of low - frequency and high - frequency segment of colon and contractive complex myoelectric hyperactivity in the patients of the hyperactive liver - Qi attacking the spleen; hyperelectroactivity of high - frequency segment of the ascending and descending colon and contractive complex myoelectric hyperactivity and decrease of electric activity of high - frequency segment of the sigmoid colon in the patients of loose stool due to hypofunction of the spleen: obvious decrease of electric activiy of low - frequency and high-frequency of the colon and contractive complex myoelectric hyperactivity in the patients of constipation due to deficiency of Qi or Qi - stagnation, but electric activity of the high -frequency segment of the colon and contractive complex myoelectric activity after meal in the patients of constipation due to Qi -stagnation was stronger than that in the patients of constipation due to Qi - deficiency. Electric frequency spectrum of colon can indirectly reflect changes of colon dynamics of different syndromes, and it can partially expain the changes of stool and the mechanism of relative symptoms.

Objective To investigate the protective effects of low-molecular heparin and urokinase on glomerular inflammation. Methods Forty 3-month-old female Wistar rats were randomly divided into five groups with 8 rats in each group: normal control (NC) group; lipopolysaccharide (LPS) group; LPS and tranexamic acid (LT) group; LPS, tranexamic acid and low-molecular heparin (LTH) group; and LPS, tranexamic acid and urokinase (LTU) group. Fibrin deposition and CD11b positive cells were identified by immunohistochemical staining. Western blotting was used to determine the ICAM-1 expression. Results No fibrin depositions and CD11b positive cells were found in glomeruli of rats in NC group. Compared with NC group, fibrin deposit (9.1%?1.6%), CD11b positive cell (11.2?2.1) and ICAM-1 expressions (0.23?0.09) were significantly increased in L group (P0.05). Conclusion Both low-molecular heparin and urokinase can effectively decrease fibrin deposits and alleviate inflammation.

OBJECTIVE To study the clinical effect of preventing and treating sterile phlebitis(abacterial phlebitis,SP) by infrared irradiation after peripherally inserted central catheterization(PICC).METHODS Totally 122 inpatients from the Zhejiang Provincial People′s Hospital were divided into two groups randomly,the control group and the experimental group.The control group was only treated with usual care after PICC,while the experimental group with both infrared irradiation and usual care.The incidence rate of SP and its correlation factor were compared between the two groups.RESULTS None in the experimental group got SP.Nevertheless,the control group got a 21.9%incidence rate.The development of SP mostly own to impassable intubation or repeating intubation.CONCLUSIONS For those patients with impassable intubation or repeating intubation during PICC operation,infrared irradiation shows greatly helpful and can obviously prevent the SP and diminish patients′ fee.

Objective To compare the degree of atherosclerosis in middle-aged versus elderly patients with chronic kidney disease (CKD),and to explore the relationship between inflammatory molecules in venous blood and atherosclerosis.Methods Totally 87 elderly patients and 64 middleaged patients with CKD were selected.The vein fasting blood samples were taken in the morning.Serum levels of interleukin (IL)-18,IL-6,tumor necrosis factor (TNF-α),high-sensitivity C-reactive protein (hs-CRP) were measured by enzyme-linked immunosorbent assay (ELISA) and ankle-brachial pulse wave conduction velocity (baPWV) was detected.Results Inflammatory molecules such as IL-18,IL-6,TNF-α and hs-CRP levels in venous blood were increased with the severity of CKD in both two groups,and the levels of inflammatory molecules were higher in elderly group than in middle-aged group.There was a signifiant difference in the detection rate of patients with baPWV＞1400 cm/s between elderly group and middle-aged group [30 cases (34.5％) vs.11 cases (17.2％),x2 =5.58,P＜0.05].The prevalence of PWV＞1400 cm/s was higher in patients with hyperlipidemia and hypertension than in patients with diabetes or heart disease (elderly group:100％,100％ vs.45.5％,40％; middle-aged group:80％,100％ vs.28.6％,33.3％,respectively; P＜0.05).The inflammatory protein (IL-18,IL-6,TNF-α and hs-CRP) levels were significantly higher in patients with baPWV＞ 1400 cm/s than those with baPWV ＜ 1400 cm/s (P＜0.05).Conclusions The degree of atherosclerosis is more severe in elderly CKD patients than in middle-aged CKD patients,which needs to be taken seriously enough.

Objective To investigate hearing and speech rehabilitation effects of music therapy for prelingual-ly deaf children with cochlear implantation.Methods A total of 107 cases of prelingual deaf children with cochlear implantation were selected and divided into control group (n=50)and observation group (n= 57 ).The control group was given conventional hearing and speech rehabilitation training while the observation group was given more music therapy.Before and 3 months,6 months and 12 months after rehabilitation training,the auditory behavior level (CAP),speech understand the grading (SIR)and the auditory speech recognition ability from the 2 groups were compared.ResuIts Before training,CAP of the observation group was 2.85±0.02 level,SIR was 2.92±0.03 and hearing and speech recognition ability was 59.51±5.41,while the control group yielded levels of 2.92±0.03, 1.05±0.02 and 58.99±4.31 respectively.The difference between the two groups was statistically insignificant (P>0.05).After training for 3,6,and 12 months,the two groups had different degrees of improvement in hearing and speech rehabilitation:the observation group in the CAP was up to 6.95±0.34 level,SIR up to 4.37±0.09 level,and hearing and speech recognition capacity up to 92.03 ± 11.32,showing significantly more improvements than those of in control group (P<0.05).ConcIusion Music therapy can significantly improve the effects of the hearing and speech rehabilitation training in cochlear implantation in prelingually deafened patients.The auditory behavior after rehabilitation training classification,speech intelligibility rating and auditory speech recognition ability can also be improved.

Objective To evaluate the diagnostic value of the combination of serum DKK1 (Dickkopf-1 )and P53 autoantibodies in patients with esophageal squamous cell carcinoma (ESCC).Methods Serum levels of DKK1 and P53 autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) for the 1 26 patients with ESCC and 60 normal controls.Receiver operating characteristics (ROC)was used to calculate the diagnostic efficiency.Results The serum levels of DKK1 and P53 autoantibodies were signifi-cantly higher in ESCC than those in normal controls [(673.09 ±343.82)pg/ml vs (362.05 ±1 48.07)pg/ml, Z =6.1 58,P <0.000 1 ;(0.398 ±0.546)vs (0.069 ±0.050),Z =3.832,P <0.000 1 ].ROC curves showed the optimum diagnostic cutoff for serum DKK1 was 588.77 pg/ml,with an area under curve (AUC)of 0.780 (95%CI:0.71 5 ~0.844,61 .9% sensitivity,95.0% specificity).Measurement of P53 autoantibodies demonstrated an AUC of 0.674 (95%CI:0.598 ~0.750,45.3% sensitivity,95.0% specificity).The com-bination of DKK1 and P53 autoantibodies yielded an AUC of 0.843 (95%CI:0.788 ~0.897,73.8% sensitiv-ity,95.0% specificity).In early-stage ESCC,combined detection of DKK1 and P53 autoantibodies improved the diagnostic power,with an AUC of 0.903 (95%CI:0.845 ~0.961 ,81 .0% sensitivity,95.0% specifici-ty).Conclusion Serum DKK1 and P53 autoantibodies can be used as potential diagnostic biomarkers for the ESCC.Combined detection of them might aid the early diagnosis of ESCC.

Objective To reduce infection risk in hemodialysis patients through analyzing the causes of over stand-ard colony forming unit(CFU)and conducting bacteriological detection of hemodialysis concentrated B solution. Methods According to microbial monitoring results of hemodialysis concentrate B solution in a hospital between November 2011 and May 2012,disinfection frequency of B solution was changed and dispensing container was covered during the process of using,four groups were divided according to different measures (group A disinfected twice a week,covered dur-ing the process of using;group B twice a week;group C once a week;group D once every two weeks),monthly bacte-riological detection of B solution was conducted,condition before and after disinfection of four groups were com-pared.Results Bacterial count in group A,B ,C and D was(25.41 ±15.08),(28.24±28.04),(68.58 ±22.58), and (75.25±26.63)CFU/mL,respectively (F =79.00,P <0.01 );bacterial count of group A,B,and C after in-tervention were all lower than group D before intervention (all P <0.01),bacterial count of group A and B was the lowest.The qualified rate of group A was 100.00%,the unqualified rate of group B,C,and D was 13.95%, 24.24%,and 35.94% respectively(χ2 =28.70,P <0.01 ),the unqualified rates of group A,B,and C after inter-vention were all lower than group D.Conclusion Hemodialysis concentrated B solution should be used within 24 hours after preparing,disinfected twice a week,and covered during the process of using ,so as to control B solution colony number within the standard level.

Objective To analyze the detection rate of HBV serological makers in non-hepatic inpatients in the past six years. Methods Serum samples of 70 582 non-hepatic inpatients from three large hospitals were collected during 2003 to 2008. Serological markers of HBV ( HBsAg, anti-HBs, HBeAg, antiHBe and anti-HBc) were detected by the AxSYM MEIA system (Abbott Laboratories,Abbott Park,IL).Combining the test results of serological makers with other clinical data, several analysis models for this retrospective study were set up to evaluate the year-to-year changes in serological makers and the detection rates of each model. Results The order from high to low of detection rate of the 5 HBV serological markers was anti-HBc (55. 17% ), anti-HBs (49. 57% ), anti-HBe (28.42%), HBsAg ( 8. 92% ) and HBeAg (2. 12% ), and all of them had a downward trend in the past six years. The positive rate of HBsAg went down from 9. 30% (2003) to 8.70% (2008). The positive rate of HBsAg among people who were born after 1992 (2. 28% ) were significantly lower than that of the overall population (8. 92% ) and fell from 3.57%(2003) to 1.85% (2008). Each detection rate of all serological makers had male sexual side effect [HBsAg ( 12. 38%/7. 25% ), HBeAg ( 2. 72%/1.58% ), anti-HBc ( 56. 57%/53.43% ), anti-HBe (41.50%/28. 35% ) and anti-HBs (65.48%/50. 00% ), male/female]. The differences were statistically significant (Chi-square values of HBsAg, HBeAg, anti-HBc, anti-HBe and anti-HBs were 509.74,105.78, 69.66, 1 321.61 and 1 726.91, respectively; all P ＜ 0. 01).Twenty-six models of HBV serological makers from 70 582 inpatients were summed up, and 8 models had positive rates geater than or equal to 1%. The "All Negative" model ranked No. 1 and had no significant change from year to year. During the past six years, models representing "A11 Negative" and "anti-HBs Positive alone" were mainly in individuals younger than or equal to 20-year-old, while the models representing "anti-HBc and/or anti-HBe,anti-HBs Positive" were mostly in people older than 20-year-old. The distribution curve of models representing "HBsAg, HbeAg and anti-HBc Positive" and "HBsAg, anti-HBc, anti-HBe Positive"etc. showed a bell-shape, covering the population from 20-year-old to 70-year-old. Conclusions The slowlydescending tendency of the detection rates of HBV serological makers was observed during the past six years.The detection rates of HBV in the younger generation decreased significantly. However, the HBV infection rates of overall population is still high, so it is a high time that we made continuous improvement for the serum HBV screening technique in order to reduce the HBV infection ratess.