Objective To further improve the morphological materials of AGs by micro-dissection, histology and CT, we observed the arachnoid granulations (AGs) in middle cranial fossa. Methods Thirty-three adult cadaveric heads were used for microsurgical dissection;Histological sections of AG specimens from 3 cadaver heads were examined. Forty patients who had both normal conventional brain CT and computed tomographic venography (CTV) were retrospectively reviewed. Results In middle cranial fossa the AGs occur in the following situations in order of frequency: the middle meningeal sinus, sphenoparietal sinus, lateral foramen rotundum and cavernous sinus. AGs usually show round, oval in shape and irregular in shape. AGs can be divided into individual type and leaflet type under light microscope. The numbers of AGs were observed by microanatomy and CTV were 8.72 and 3.52 respectively. The AGs of cavernous sinus was not localized precisely on CTV. Conclusion Study of the AGs in the middle cranial fossa systematically and comprehensively enriches anatomy and image knowledge. It is helpful in neurosurgical planning and choosing operalion procedure to avoid postoperative complications.

Objective To analysis the long-term retention rate of Levetiraceram (LEV) monotherapy or combination therapy of infant epilepsy. Methods The clinical data of patients with infant epilepsy treated by LEV had been retrospectively analyzed from July 2006 to June 2007. Results Sixty patients with infant epilepsy treated by LEV had been recruited, 20 cases with partial seizures, 19 cases with generalized seizures, 21 cases with epilepsy syndrome. Among them 21 cases was intractable epilepsy. The retention rates of LEV in 6-month, 1-year, 2-year, 3-year and 4-year were 95.5%, 75.0%, 60.0%, 51.7%, and 38.3%. The most common reason for withdrawal was lack of effect (43.2%). COX regression model suggested that duration>1 month (RR=2.91, 95%CI:1.16~7.30) and refractory epilepsy (RR=2.30, 95%CI:1.22~4.32) were risk factors of withdrawal (all P<0.05). After treatment, the seizure frequency signiifcantly reduced compared with baseline (P<0.01). To the end of the follow-up, the efifciency was 100%and the complete remission rate was 69.57%in 23 cases continued treatment. The main side effect were fatigue (56.0%), and sleep increased, irritability, and so on. Conclusions LEV monotherapy or combination therapy has well long-term retention rate, maintains well efifcacy and tolerability in infant epilepsy.

Objective:To combine the detection of serum levels of adenosine deaminase (ADA) and T-cell spot test (T-spot.TB),and to explore their significances in diagnosis of pulmonary tuberculosis.Methods:159 patients suspected with pulmonary tuberculosis were selected and divided into pulmonary tuberculosis group and non-tuberculosis group (n=68);80 healthy people were randonly selected as healthy control group.The serum ADA levels and number of T-spot of the subjects in three groups were detected.Ther serum ADA levels and the positive rates of T-spot.TB in various groups and their sensitivities and specifities were compared. Results:The serum ADA level of the patients in pulmonary tuberculosis gruop was (22.10±6.60)U·L-1;those in non-tuberculosis group and healthy control group were (16.90±6.35)and (8.70±5.98)U·L-1;the serum ADA level in pulmonary tuberculosis group was significantly higher than those in non-tuberculosis group and heathy control group (P<0.05).The positive rate of serum ADA level in diagnosis of pulmonary tuberculosis was 56% and the T-spot.TB positive rate in diagnosis of pulmonary tuberculosis was 87.9%. Combined use of parallel test, the detection sensitivity was 91.2%;using the series of joint tests,the specificity was 94.6%.Conclusion:Combined detection of serum level of ADA and T-spot.TB can significantly improve the clinical diagnosis efficacy of pulmonary tuberculosis.

Objective To explore whether dithiothreitol(DTT)is helpful for PreScission Protease to cut off the GST from GST?CT3 protein. Meth?ods The pGEX?6P?3/CT3 recombinant plasmid was transfected into Escherichia coli BL21,and the GST?CT3 fusion protein was purified by B?PER method. PreScission Protease was applied with 10 mmol/L DTT to cut off the GST,then the SDS?PAGE was performed for identification of the CT3 protein. Results Without DTT,it was very difficult for PreScission Protease to cut off the GST from GST?CT3 protein. However,in the pres?ence of 10 mmol/L DTT,PreScission Protease could cut off the GST easily as identified by SDS?PAGE. Conclusion 10 mmol/L DTT can help Pre?Scission Protease to cut GST from GST?CT3 protein,so as to achieve high concentration of CT3.

BACKGROUND:Bone cement has certain toxic effects on the human body. The probability of renovation is high after bone cement total hip arthroplasty. It is reported that the long-term effect of cementless total hip arthroplasty is apparently better than bone cement total hip arthroplasty, and can be renovated conveniently. OBJECTIVE:To investigate the clinical effect of cementless total hip arthroplasty on hip joint disease, and to compare the difference between unilateral replacement and bilateral replacement. METHODS: Clinical and folow-up data of 233 patients (280 hips), who were treated with cementless total hip arthroplasty in the Department of Orthopedics, Wuzhou Worker’s Hospital, Seventh Affiliated Hospital of Guangxi Medical University from July 2007 to December 2013, were retrospectively analyzed. According to the replacement program, they were divided into unilateral replacement group (n=186) and bilateral replacement group (n=47). Harris score of hip joint, visual analog scale score of thigh pain, the excelent and good rate of hip joint during final folow-up and complications were compared between the two groups before replacement, at 6, 12 and 24 months after replacement. RESULTS AND CONCLUSION:No significant difference in Harris scores was detected before replacement, at 6, 12 and 24 months after replacement (P > 0.05). Harris score was significantly higher at 6, 12 and 24 months after replacement compared with that before replacement in both groups (P < 0.05). No significant difference in the excelent and good rate was detected in the unilateral replacement group (87%) and the bilateral replacement group (86%) (P > 0.05). No significant difference in the visual analog scale score was seen before replacement, at 6, 12 and 24 months after replacement (P > 0.05). Visual analog scale scores were significantly lower at 6, 12 and 24 months after replacement than that before replacement in the two groups (P < 0.05). There was no significant difference in the incidence of complications after replacement in patients of both groups (P > 0.05). These findings confirm that the effects of cementless total hip arthroplasty for hip joint disease are evident, can effectively restore hip joint function. No significant difference was detected between unilateral replacement and bilateral replacement. Strict replacement operation and matching of prosthesis and medulary cavity can effectively reduce thigh pain after replacement.

Objective To investigate the changes and mechanisms of immune function after ischemic stroke by studying the reaction of C57BL/6mice's lung and spleen after ischemic stroke.Methods Sixteen mice C57BL/6 were randomly assigned to experiment group (n=8) and control group (n=8).The acute ischemic stroke model was established in experiment group by Middle Cerebral Artery Occlusion (MCAO) and no stroke was performed in control group.Then we observed the lung inflammation by HE staining of the lung.And we measured the spleen weight,spleen weight index,the count and the apoptosis index of spleen cells of mice in two groups.Results Compared with the control group,the count of the inflammatory cells in lung of mice in the experiment group was higher,the spleen weight,spleen weight index and spleen cells count of the experiment group were decreased and the apoptosis index of spleen cells of the experiment group was higher.Conclusion The mice in the experiment group have inflammatory reaction in lung,the spleen atrophied and the apoptosis index of spleen cells is increased,which suggest that stroke-induced apoptosis of immunocytes may cause immunodepression after ischemic stroke and easily lead to the infection.

Objective To construct plasmid vectors of calmodulin(CaM)Mg2+binding site mutants,and to express,purify and identify the mutant proteins. Methods Three kinds of cDNAs coding for the mutated CaM were cloned into pGEX?6P?3 plasmid vectors. These recombinant plasmids were transfected into Escherichia coli BL21 to express GST fusion proteins of CaM mutants. The fusion proteins were purified with Glutathione?Sep?harose 4B beads and PreScission protease. Results Both enzyme digestion analysis and DNA sequence identification proved the successful con?struction of the CaM mutant plasmids. SDS?PAGE results showed the high purity of each CaM mutant protein. The concentrations of three CaM mu?tants were around 1.0 mg/mL. Conclusion Prokayotic expression vectors of CaM Mg2+binding site mutants were successfully developed,and the eli?gible CaM mutant proteins were obtained. This study provided an important basis for further study on CaM’s biological function.

This study determined the effect of hepatitis B virus (HBV) replication in peripheral blood mononuclear cell (PBMC) from HBsAg-positive mothers on HBV intrauterine transmission.A total of 150 HBsAg-positive mothers and their neonates were recruited in this study.Within 24 h after birth,HBV serological markers,serum HBV DNA,PBMC HBV relaxed circular DNA (rcDNA),and covalently closed circular DNA (cccDNA) were measured in the HBsAg-positive mothers and their neonates before passive-active immune prophylaxis.The relationship between HBV replication in PBMC and HBV intrauterine transmission was examined through Chisquare test and logistic regression.The rate of HBV intrauterine transmission was 8.00％ (12/150) in the 150 neonates born to HBsAg-positive mothers.The positivities of PBMC HBV rcDNA and cccDNA in the HBsAg-positive mothers were 36.67％ (55/150) and 10％ (15/150),respectively.Maternal PBMC HBV cccDNA was a risk factor of HBV intrauterine transmission (OR =6.003,95％ CI:1.249-28.855).Maternal serum HBeAg was a risk factor of PBMC HBV rcDNA (OR =3.896,95％ CI:1.929-7.876) and PBMC HBV cccDNA (OR =3.74,95％ CI:1.186-11.793) in the HBsAg-positive mothers.Administration of hepatitis B immune globulin was a protective factor of PBMC HBV cccDNA (OR =0.312,95％ CI:0.102-0.954) during pregnancy.The positivity of PBMC HBV rcDNA was related to that of cccDNA in the HBsAg-positive mothers (x2=5.087,P =0.024).This study suggests that PBMC is a reservoir of HBV and an extrahepatic site for virus replication and plays a critical role in HBV intrauterine transmission.

BACKGROUND: The most common soft liners currently used in the clinic are silicone rubbers and acrylics, but both of them have deficiencies. Therefore, the development of soft lining materials with stability, excellent performance, and antibacterial properties is of important clinical significance. OBJECTIVE: To prepare a soft lining material with high elasticity on the basis of excellent characteristics of Eucommia ulmoides gum, and to preliminarily explore its water absorption and solubility. METHODS: The basic formula was made based on the existing formula of elastic Eucommia ulmoides gum and medical rubber, in combination with the specific requirements of denture lining materials. The ratio of Eucommia ulmoides to butadiene rubber was set to be 10:90, 30:70, 50:50, 70:30 and 90:10, and the new Eucommia ulmoides gum composite soft denture liners were then prepared. Acrylate self-curing soft lining material and Silagum silicone rubber denture soft lining material served as controls. Afterwards, the samples were put into distilled water or artificial saliva for 7 and 30 days, and the value of water absorption and water solubility were calculated. RESULTS AND CONCLUSION: (1) In the artificial saliva for 7 and 30 days, the water absorption rate and solubility of samples in different ratio groups were close to those in the Silagum group, but extremely lower than those in the acrylate group. (2) In the distilled water for 7 and 30 days, the water absorption rate and solubility of samples in different ratio groups were close to those in the Silagum group, but extremely lower than those in the acrylate group. (3) The water absorption rate and solubility of the acrylate group were significantly higher than those in the other groups at 7 and 30 days of immersion in the artificial saliva or distilled water (P < 0.05). (4) After 7 and 30 days of immersion, the water absorption rate of the same sample in the artificial saliva was significantly lower than that in the distilled water. Wile at 7 days of immersion, the samples in the 30:70 and 50:50 groups showed a lower solubility in the artificial saliva than in the distilled water (P < 0.05), and at 30 days of immersion, the samples in the 30:70, 50:50, 70:30 and 90:10 groups showed a lower solubility in the artificial saliva than in the distilled water (P < 0.05). (5) In the artificial saliva, there was no significant difference in the solubility in the 50:50 and 70:30 groups at 7 and 30 days of immersion (P> 0.05), while the solubility in the other groups exhibited significant differences at different observational times (P < 0.05). In the distilled water, there was no significant difference in the solubility in the 10:90 and 30:70 groups at 7 and 30 days of immersion (P> 0.05), while the solubility in the other groups exhibited significant differences at different observational times (P < 0.05). To conclude, the water absorption of Eucommia ulmoides gum composite soft denture liner is close to Silagum, but significantly less than that of acrylate soft lining materials, and the solubility remains at a very low level.