Objective To investigate the subchronic toxicity of a Chinese medicine Danhong injection in dogs . Methods Twenty-four healthy Beagle dogs (body weigh 7-9 kg, male:female=1:1) were used in this study.The test dogs were administered with Danhong injection by intravenous injection once every day for consecutive 6 days/week for thirteen weeks.The Danhong injection was used in 3 doses:494,247,and 124 mg· kg-1, repectively.The control dogs received normal saline injection in the same volume .The body weight, blood biochemistry, hematology, viscera relative weight and histopathology were determined for the overall toxicity assessment .Results The dogs administered with 494 g· kg-1 Danhong injection showed significantly reduced body weight gain , abnormal increase of urea nitrogen ( BUN ) , creatinine (CR) levels, and histopathological changes in the liver .Conclusions Danhong injection (494mg· kg-1 ) administered Intravenously has obvious toxicity on the liver and kidney function .

Eleven patients with small or comminuted displaced tibial avulsion fractures of posterior cruciate ligament admitted in the Department of Orthopaedics,Second Affiliated Hospital,Fujian Medical University between January 2003 and January 2008 were selected.With diameter of 4.0 mm,the length of 35 mm or 40 mm cancellous bone screw,and internal diameter of 4.0 mm spiked washer was implanted for fixation.For cases of large comminuted facture,two cancellous bone screws and spiked washer were used.Postoperatively,the limb was immobilizated in a cast plaster at a 30 degrees flexion for 2-4 weeks,followed by active exercise rehabilitation.All 11 patients were followed up with an average of 18 months(ranged 7 to 36 months).No electrolysis,corrosion reaction to metal material,or allergic response to the spiked washer were detected in patients.At 2 months,all patients achieved bony union,no fragment redisplacement,no knee laxity or instability was detected,and the affected knees regained full range of motion.The knee function was significantly improved.

Objective To compare the clinical efficacy of anatomic locking compression plate augmented with anchor nail versus calvicular hook plate in the treatmeut of Neer type Ⅱb distal calvicular fractures.Methods The data of 65 patients were retrospectively analyzed who had undergone surgery in our hospital from January 2012 to January 2015 for Neer type Ⅱb distal calvicular fractures.They were 39 men and 26 women,aged from 18 to 58 years(average,42.6 years).Open reduction plus anatonical locking compression plating augmented with anchor nailing was performed in 33 patients (group A) and open reduction plus calvicular hook plating was conducted in 32 (group B).The internal fixation was removed oue year after surgery in all.Constant Scale was used to evaluate shoulder function at 1,3 and 6 months postoperatively.X-ray examination on the shoulder was also conducted to observe fracture healing.Complications and percentage of the patients who resumed their job 3 months postoperatively were documented.Results The 2 groups were comparable because they showed no significant differences in general clinical information preoperatively (P ＞ 0.05).All the 65 patients were followed up tor 12 to 18 months (average,15.2 months).The fracture clinical healing time in group A (23.9 ± 2.3 weeks) was significantly shorter than in group B (26.1 ± 3.0 weeks) (P ＜ 0.05).The mean Constant-Murley scores at 1,3,6 months and the last follow-up postoperatively in group A (91.2 ±3.6,95.2 ±2.4,96.1 ±5.1 and 97.3 ± 1.6) were significantly higher thau those in group B (89.2 ± 6.1,91.1 ± 1.1,91.2 ± 6.2 and 92.1 ± 3.1) (P ＜ 0.05).The rate of total complications in group A (6.1％,2/33) was significantly lower than in group B (25.0％,8/32) (P ＜ 0.05).At postoperative 3 months,31 patients (93.9％) in group A resumed their job while 23 (71.9％) ones did in group B,showing a significant difference (P ＜ 0.05).Conclusions For Neer lⅡb distal calvicular fractures,anatomical locking compression plate augmented with anchor nail is obviously superior to calvicular hook plate,because the former can avoid damage to the soft tissue surrounding the acromion,leading to satisfactory functional recovery of the affected shoulder.

Objective To explore the influences of sufentanil and different dose midazolam coprocessing on myocardial injury in rats during myocardial ischemia-reperfusion (IR ). Methods Forty two healthy male SD rats were selected and randomly divided into 7 groups including,group A:rats were treated with sham operation;groups B0-B5:rat IR model was con-structed;group B0:rats were untreated with sufentanil or midazolam;group B1:rats were per-trea-ted with 3 μg/kg sufentanil;group B2:rats were post-treated with 0.1 mg/kg midazolam;group B3:rats were post-treated with 0.3 mg/kg midazolam;group B4:rats were per-treated with 1.5 μg/kg sufentanil and post-treated with 0.05 mg/kg midazolam;group B5:rats were per-treated with 1.5μg/kg sufentanil and post-treated with 0.15 mg/kg midazolam.Sufentanil was injected through cervi-cal vein before ischemia.Midazolam was injected through cervical vein after ischemia.After reperfu-sion,arterial blood was collected for detecting the activity of CK-MB,LDH or SOD,and the concen-tration of MDA,TNF-α,or IL-6.Rat hearts were picked off to measure the severity of myocardial in-farction (IS/AAR).The expressions of Bax,cleaved-caspase-3 and Bcl-2 in myocardial tissues were detected by Western blots.Results CK-MB activity,LDH activity,MDA concentration,TNF-αlev-el,IL-6 level,Bax and cleaved-caspase 3 expression levels in groups B0-B5 were obviously higher than those in group A (P <0.05),in groups B1-B5 were obviously lower than those in group B0 (P<0.05),in groups B3 and B4 were obviously lower than those in group B2 (P <0.05),in group B5 were obviously lower than those in groups B1-B4 (P < 0.05 );values of IS and IS/AAR (%)in groups B1-B5 were obviously lower than those in group B0 (P <0.05),in groups B3 and B4 were ob-viously lower than those in group B2 (P < 0.05 ),in group B5 were obviously lower than those in groups B1-B4 (P <0.05 );SOD activity and Bcl-2 expression level in groups B0-B5 were obviously lower than those in group A (P <0.05),in groups B1-B5 were obviously higher than those in group B0 (P <0.05),in groups B3 and B4 were obviously higher than those in group B2 (P <0.05 ),in group B5 were obviously higher than those in groups B1-B4 (P <0.05).CK-MB declined when com-pared with group B0;LDH declined;SOD rose;MDA declined;TNF-αdeclined;IL-6 declined;IS/AAR declined;Bcl-2 expression rose to 2.25 fold;Bax expression dropped to 54.89%;cleaved-caspase-3 expression dropped to 49.67%.Conclusion Sufentanil pretreatment combined with mid-azolam aftertreatment can significantly alleviate rat myocardial IR injury,which plays more effective cardioprotective effects than being used alone.