[Objective] To observe the effect of therapy of strengthening spleen to eliminate blood-stasis and fluid dampness for ascites due to liver cirrhosis. [Methods] Sixty - five cases were randomly allocated to Group A and Group B by interval randomization. Group A was treated with Jianpi Xiaogu Decoction (mainly composed of Radix Codonopsis, Polyporus, Rhizoma Atractylodis Macrocephalae, Pericarpium Arecae), one dose per day, which is combined with routine western medicine when necessary and Group B was treated with routine western medicine alone. The treatment course lasted one month and then a half-a-year follow- up was carried out. [Results] After treatment, 3 was cured, 12 markedly effective, 18 effective and 2 ineffective in Group A and 1,5, 14 and 10 respectively in Group B. The differences of each ranked effect in the two group were significant (U=2.80, P=0.005) . So did the total therapeutic effect (x2 = 8.19, P

Based on the digital and network environment, this paper analyzes the problems existing in the teaching of traditional orthopedics in medical colleges and universities, including single teaching form, neglecting the students' practical skills and the phenomenon of students' poor learning initiative. It points out that the orthopedics teaching under the digital and network environment should be integrated through interactive teaching, multimedia teaching, WeChat network teaching, simulation teaching, practice teaching and other teaching forms and should pay attention to the reasonable use of teaching methods, make clear teachers and students' subject status, pay attention to students' learning ability training, so as to improve the teaching quality of orthopedics in medical colleges.

Anticancer drugs are not accessible to hypoxic regions in human solid tumors and which are not sensitive to radiotherapy,but they provide a haven for anaerobic bacteria.Anaerobic bacteria have the tendency of migrating to hypoxia areas of tumor.So they not only can be used as tumor-specific vectors for gene therapy but also be administered together with other anticancer treatments to enhance their antitumor efficacy.Bacterial oncolysis therapy which are based on anaerobic bacteria has great promise as anticancer therapy.

Objective To investigate the effect of has-miR-663 on proliferation and apoptosis of human adrenal cortical cell line SW-13.Methods Based on the first stage study,in adrenocorticotropinindependent macronodular adrenal hyperplasia (AIMAH),hsa-miR-663 was up-regulated.The synthetic hsa-miR-663 mimics and inhibitors were transfected the SW-13 cell line.Real-time quantitative PCR (RT-PCR) tested the effect of the transfection.According to the results of RT-PCR,cell line was divided into five groups,including blank group,mimics NC group,inhibitor NC group,mimics group,inhibitor group.Then proliferating of the cell was tested by MTT and the apoptosis by Annexin V-FITC/PI.Result By RT-PCR,mimics group showed that has-miR-663 was over-expressed,and inhibitor group were knocked down.MTTshowed that,compared with NC group and Blank group,SW-13 cell line proliferated faster in mimics group and slower in inhibitor group.Annexin V-FITC/PI showed that apoptosis of SW-13 cell line was not different in all the five groups.Conclusion Up-regulating has-miR-663 could accelerate proliferation of adrenal cortical cells.The microRNA might play some role in the pathogenesis of AIMAH.

Objective To investigate the imaging features of chest CT and characteristics of laboratory tests in microscopic polyangitis (MPA) with pulmonary infection. Methods Based on the results of sputum culture, 42 patients with MPA were divided into two groups, MPA with pulmonary infection (16 cases)and MPA without pulmonary infection (26 cases). The results of chest CT images and laboratory tests were compared between the 2 groups by χ2 test (or Fisher exact test) and student t test (or Mann-Whitney U test), respectively. Results Compared with patients without pulmonary infection, patients with pulmonary infection showed patchy opacities(15/16 cases versus 14/26 cases,χ2=5.631,P=0.018)and consolidations(7/16 cases versus 2/26 cases,χ2=5.657,P=0.017)more frequently. Patchy opacities showed peripheral distribution less frequently in the MPA with pulmonary infection group than in the MPA without pulmonary infection group (6/15cases versus 12/14 cases,P=0.021). Patients with pulmonary infection showed the same frequent as the patients without pulmonary infection in the following CT findings including ground glass opacities, pleural effusions, mediastinal adenopathy and pulmonary fibrosis (P>0.05). The MPA with pulmonary infection group had higher neutrophil ratio than the MPA without pulmonary infection group (0.84 ± 0.12 versus 0.74 ± 0.11,t= 2.845,P= 0.007). Conclusions The MPA patients with pulmonary infection show patchy opacities and consolidations more frequently, and have higher neutrophil ratio than the MPA without pulmonary infection. The characteristics can be helpful in the diagnosis of pulmonary infection in the MPA patients and guild the treatment.

OBJECTIVE: To investigate the effects of carvedilol on blood pressure in hypertension patients and vascular endothelial function. METHODS: 100 inpatients with primary hypertension were collected from our hospital from Jan. 2008 to Jan. 2010 and randomly divided into treatment group and control group(n=50). Treatment group were given initial oral dose of carvedilol tablets 6.25 mg twice a day for 7～14 days if it was tolerated; the dose of carvedilol tablets increased to 12.5 mg or 25 mg twice a day according to blood pressure at trough concentration if it was necessary. Control group were given carvedilol tablets 6.25 mg twice a day if it was tolerated. After 1 week, control group received double dose of metoprolol with maximum dose of carvedilol of 50 mg. The treatment course was 3 months. The contents of NO and ET in serum of 2 groups were detected. Antihypertensive effect and effects of carvedilol on vascular endothelial dilatation and systolic function were observed and compared between 2 groups. RESULTS: After treatment, the systolic and diastolic blood pressure in both groups were decreased significantly(P

Objective To summarize our experiences on open heart surgery through the right subaxillary small incision. Methods Between March 2001 and June 2008,612 patients with congenital heart diseases were treated by open surgery trough a right subaxillary small incision via hypothermic cardiopulmonary bypass. The incision was made from the posterior axillary line at the axilla to the middle axillary line a the fifth intercostal space (4-8 cm in length),and then the intercostal muscle was cut at the inferior edge of the fourth rib for the entrance into the chest cavity. In this series,165 patients had ASD,403 had VSD,35 had ASD complicated with VSD,3 showed PECD,and 6 were found as PAPVC. Results The mean cardiopulmonary bypass time was (25.0?5.5) min,402 of the patients (65.6%) need no blood transfusion. The mean hospital stay was (6.0?4.5) d,no patient died in hospital. The 420 patients were followed up for 5 to 80 months (mean 47 months). During the period,cardiac ultrasonography showed good outcomes in the patients. No complications or death were detected. Conclusions Right subaxillary small incision is a safe and minimally invasive method for the treatment of ASD and VSD with quick recovery,less hemorrhage,shorter hospital stay,less complications,and satisfying cosmetic outcomes.

Objective To evaluate the efficacy of the end point of catheter ablation of pulmonary vein (PV) foci initiating atrial fibrillation (AF) to guide the catheter ablation of PAF Methods Thirty two drug resistant PAF patients withdrew all antiarrhythmias drugs for one week and received Holter monitoring on dyas 7, 5, 3, and 1 before operation, respectively During the operation, focal ablation was performed in the patients whose target PV could be documented, and all pulmonary veins os and isthmus of right atrium were ablated in the other patients All patients received Holter monitor on days 1,3,5, and 7 postablation, respectively If PAF relapsed after the first operation, the patient would undergo operation again until no AF was documented The patients were followed up for 3 to 20 months Results Total 82 PV in 32 patients were success isolated In foci ablation patients, 9(60 0%) patients successed by one operation, and 6(40 0%) patients successed after the second operation In all PVs isolation patients, 9(47 0%) patients successed by one operation, and 10 (58 8%) patients successed after the second operation, and the success rate was 11 (64 7%) after the third operation The overall success rate was 81 3% Conclusion The endpoint of PAF ablation should be no AF recurrence when no antiarrhythmias drugs were administrated The success rate was higher in the patients who had clear target PV and the overall success rate could be improved by the ablation once again

Objective: To investigate prevalence of neurosis in a college of central China. Method:4465 college students were investigated by epidemiological method used in the national survey of 12 areas 10 years before. Result: The present prevalence of neurosis in students were 9.34%, higher than that of the national survey 10 years before (6.44%). In different diagnoses, the present prevalence of anxiety disorder was much higher. Male students had higher rate of neuroses than female, those in grade 3 or 4 had higher rate of neuroses than those in other grades. The most obvious invoke factor of neurosis was lovelorn. Conclusion: The prevalence of neurosis is higher than 10 years before.

Objective To evaluate CT-guided pereutaneous biopsy and fine needle aspiration in diagnosing malignant musculoskeletal tumors. Methods CT-guided percutaneous biopsy and fine needle aspiration was performed in 106 cases with suspected musculoskeletal tumor. The pathological results obtained from biopsy specimens were compared with clinical final pathological diagnoses. Results All 106 cases were finally diagnosed as suffering from malignant musculoskeletal tumors, which were proved by operation and incisional biopsy. Of 106 cases receiving CT-guided percutaneous biopsy and needle aspiration, the pathologic diagnosis obtained from this procedure was consistent with the clinical final pathological diagnosis in 89 and was "negative" in 17, with an accuracy rate of 84.0%. Conclusion CT-guided percutaneous biopsy and fine needle aspiration is a safe, simple and effective technique for the diagnosis of malignant musculoskeletal tumors, Incisional biopsy may be necessary when this procedure gives "negative" result.