1.Effects of bovine plasma fibronectin on the proliferation and differentiation of rat osteoblasts in vitro
Xue MING ; Zhang YING ; Zhong MING
Journal of Practical Stomatology 2000;0(06):-
Objective: To investigate the effects of bovine plasma fibronectin on the proliferation and differentiation of rat osteoblasts. Methods:Rat osteoblasts were cultured and identified. AlamarBlue were used to determine the effect of various concentration of bovine plasma fibronectin on the proliferation of the osteoblasts. ELISA method was used to determine the alkaline phosphatase activity of the cells. Results: Fibronectin stimulated the proliferation of rat osteoblasts at 40 ?g/ml (P
4.Effects of adriamycin on cultured mouse podocytes VEGF expressions and the potential protective effects of dexamethasone.
Xiao-zhong LI ; Hai-tao YUAN ; Xue-guang ZHANG
Chinese Journal of Pediatrics 2003;41(2):146-146
Animals
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Antibiotics, Antineoplastic
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pharmacology
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Blotting, Northern
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Cells, Cultured
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Dexamethasone
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pharmacology
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Doxorubicin
;
pharmacology
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Enzyme-Linked Immunosorbent Assay
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Gene Expression Regulation
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drug effects
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Glucocorticoids
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pharmacology
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Kidney Glomerulus
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cytology
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drug effects
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metabolism
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Mice
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RNA, Messenger
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drug effects
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genetics
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metabolism
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Vascular Endothelial Growth Factor A
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genetics
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metabolism
5.Effects of perindopril on bone metabolism in a rat model of retinoic acid-induced osteoporosis
Yi ZHONG ; Qing XUE ; Yi ZHOU ; Lianghua CHEN ; Lichao ZHANG
Chinese Journal of Tissue Engineering Research 2016;20(18):2589-2595
BACKGROUND: Renin-angiotensin-aldosterone system existed in bone tissue. Recent studies on antihypertensive drugs found that angiotensin converting enzyme inhibitor type antihypertensive drug was possibly effective for osteoporosis. Perindopril is one of the commonly used antihypertensive drugs. Whether perindopril affected bone metabolism or could be used in anti-osteoporosis has not been reported.
OBJECTIVE: To observe effects of perindopril on bone metabolism in a rat model of osteoporosis induced by retinoic acid.
METHODS: Fifty Sprague-Dawley rats were randomly divided into five groups, with ten in each group. In the model group and each perindopril groups, rats were intragastricaly administered retinoic acid solution 80 mg/kg per day. After successful model establishment, rats in different perindopril groups were intragastrical y administered perindopril 2, 4 and 8 mg/kg per day, once a day, for 42 consecutive days. In the normal control and model groups, rats were given an equal volume of distil ed water. Serum calcium, phosphorus, alkaline phosphatase, bone mass and bone mineral density were detected in each group. Expression of bone specific alkaline phosphatase and tartrate-resistant acid phosphatase mRNA in bone tissue was determined.
RESULTS AND CONCLUSION: Compared with the model group, after treatment with perindopril, serum calcium and phosphorus levels were increased, alkaline phosphatase activities were significantly decreased, bone mass and bone mineral density were obviously increased in rats with retinoic acid-induced osteoporosis. Expression of bone specific alkaline phosphatase mRNA was higher in the perindopril 8 mg/kg group than in the perindopril 2 and 4 mg/kg groups and model group. Tartrate-resistant acid phosphatase mRNA expression was higher in the perindopril 8 mg/kg group than in the model group. These results indicated that perindopril could improve partial bone metabolic biochemical markers in osteoporosis rats, promoted bone formation by up-regulating bone specific alkaline phosphatase mRNA expression, and had a certain preventive effect on retinoic acid-induced osteoporosis.
6.Expression of VEGF mRNA induced by angiotensinⅡ in human hepatic cancer cell line
Jian ZHANG ; Xue XING ; Hongguang HAN ; Zhong GE
Chinese Journal of General Surgery 1993;0(02):-
Objective To investigate the effects of angiotensin Ⅱ (Ang Ⅱ) on the expression of vascular endothelial growth factor (VEGF) in human hepatic cancer cell line Hep G2.Methods Cultured Hep G2 cells were treated by AngⅡ with various concentrations and were collected at different time points.Then total RNA was extracted.The expression of VEGF mRNA in cultured Hep G2 was determined by relative quantitative reverse transcription polymerase chain reaction(RT-PCR),and the proliferation was detected by methyl thiazolyl tetrazolium (MTT).Results AngⅡ stimulated the proliferation of HepG2 cells,and enhanced the expression of VEGF mRNA (P
7.Specialization Development of Medical Physics Teachers
Dongsheng ZHANG ; Weigang ZHONG ; Xiuzhen LI ; Mei XUE
Chinese Journal of Medical Education Research 2002;0(01):-
Under the condition of the inevitable development trend of teachers' specialization in the world,this paper discusses the professional quality and existing problems of the medical physics teachers and the development ways to realize the teachers' specialization in medical physics.
8.Synergistic effects of toremifene and anti-tumor drugs on human lung cancer cell lines A549
Xue-Yan ZHANG ; Qiang LI ; Zhong-Ling LIU ; Al ET
China Oncology 2001;0(05):-
Purpose:To study the activity of toremifene and its synergistic effect with anti-tumor drugs on human lung adenocarcinoma cell line A549,which might be expected to provide a new mode of therapy for the clinical management of lung cancer.Methods:The cytotoxic effects of these agents on human lung cancer cell line A549 were measured by a tet- razolium-based volorimetric assay (MTT assay).Results:Toremifene can inhibit the growth of A549 cell directly.The A value of the low dose toremifene combined with anti-tumor drugs were lower than that of anti-tumor drugs alone.Toremifene combined with VCR,ADM,DDP and VP-16 showed better effects.Conclusions:Toremifene combined with chemotherapy drugs shows significant synergistic anti-tumor effect on A549 cell.This might provide experimental evidence for lung cancer therapy.
9.Clinical analysis of Takayasu's arteritis with cardiomyopathy
Hua ZHONG ; Weiguo ZHU ; Xue LIN ; Yunyun FEI ; Wei ZHANG ; Xiaowei YAN ; Fengchun ZHANG
Chinese Journal of General Practitioners 2014;13(11):929-932
The clinical data of 13 patients diagnosed as Takayasu's arteritis with cardiomyopathy in Peking Union Hospital were reviewed.Echocardiography showed cardiomegaly (left chambers mainly),diffuse weakened myocardial wall movement and reduced left ventricular ejection fraction.Dilated cardiomyopathy was diagnosed in two patients.Glucocorticoid and cyclophosphamide were the essential medication for treatment.One patient died,1 patient was aggravated and the disease condition in remaining 11 patients was stable or improved.Takayasu's arteritis with cardiomyopathy is a rare disease and it attacks younger people.It is difficult to identify at onset,and the disease deteriorates rapidly.Echocardiography should be performed earlier to evaluate the heart function for early diagnosis and treatment.Administration of large dose of glucocorticoid and cyclophosphamide at early stage may improve the prognosis.
10.Antidepressant-like effects of ammoxetine in mice of behavioral despair model and monoamine neurotransmitter depletion model
Yan GUO ; Rui XUE ; Tingting ZHANG ; Qiongyin FAN ; Yunfeng LI ; Bohua ZHONG ; Youzhi ZHANG
Chinese Journal of Pharmacology and Toxicology 2016;30(5):498-503
OBJECTIVE To study the antidepressant effects of ammoxetine(AMX)and the underlying mechanisms. METHODS Two behavioral despair models,the tail suspension test (TST) and the forced swimming test(FST),were used to evaluate the antidepressant-like effects of AMX 2.5-20 mg · kg-1 following oral administration. Monoamine neurotransmitter p-chloro-phenylalanine(p-CPA)andα-methyl-p-tyrosine(AMPT) depletion models in mice were used to investigate the effects of AMX on levels of 5-serotomin(5-HT)and norepinephrine(NE)in the brain. RESULLTS The results of behavioral study showed that compared with normal control group,AMX(10 and 20 mg · kg-1)reduced the immobility time of mice by 51.4% and 80.7% in the TST(P<0.05,P<0.01) or by 48.0% and 66.2% in the FST (P<0.05),respectively. Locomotion activity test indicated that AMX did not increase or decrease the movement distance of mice,demonstrating that AMX had no excitatory or inhibitory actions on the central nervous system. Moreover,AMX(5,10 and 20 mg·kg-1)exerted antidepressant effects in the p-CPA induced 5-HT depletion model and AMPT induced NE depletion model,as evidenced by the significantly reduced immobility time,ie,63.9%,93.4%,90.5% and 61.9%,77.2%,100% reduction in the TST (P<0.01),respectively,and AMX at the dose of 20 mg·kg-1 significantly increased the concentrations of 5-HT and NE by 144.7% and 57.2% in the mouse brain(P<0.05) ,respectively. CONCLUSION AMX has strong antidepressant-like effects in behavioral despair models and monoamine neurotransmitter depletion models in mice,which is involved in the increased levels of 5-HT and NE in the brain.