1.Treatment of fungal septicemia in a premature infant with caspofungin.
Xiao-chun DING ; Xue-ping ZHU ; Zhi-hui XIAO
Chinese Journal of Pediatrics 2008;46(6):479-discussion 480
2.Study on anti-angiogenesis effect of three curcumin pigments and expression of their relevant factors.
Yan-fen HUANG ; Xue-xin ZHU ; Zhi-shan DING ; Gui-yuan LV
China Journal of Chinese Materia Medica 2015;40(2):324-329
To study the in vitro anti-angiogenesis effect of three curcumin pigments (curcumin, demethoxycurcumin, bisdemethoxycurcumin). In the study, the inhibitory effect of the three curcumin pigments on proliferation of HUVEC cells induced by OX-LDL and the effect on migration of HUVEC cells were detected. The effect on neovascularization was observed by chorioallantoic membrane (CAM) test. The effect on cell adhesion factors ICAM-1 and VCAM-1 of HUVECs were tested by Real-time RT-PCR. It was found that the three curcumins could inhibit the proliferation of HUVEC cells induced by OX-LDL within the dosage range 4, 8, 16 mg x L(-1), with a dose-dependence. The proliferative effect of curcumins on HUVECs was greater than the other two derivatives (P < 0.01). All of the three curcumin pigments inhibited the migration of HUVEC cells and the angiogenesis of chick chorioallantoic membrane (CAM). The migration inhibition rate of curcumins at middle and high concentrations was greater than the other two (P < 0.01). All of the three curcumin could down-regulate the expression of VEGF and ICAM-1, and curcumins showed more obvious effect in down-regulating VEGF than demethoxycurcumin and bisdemethoxycurcumin(P < 0.01); Bisdemethoxycurcumin showed the most significant effect in down-regulating ICAM-1 (P < 0.01). All of the three showed no remarkable effect on expression of VCAM-1, and only bisdemethoxycurcumin showed the down-regulating effect (P < 0.05). According to the findings, all of the three curcumin pigments could resist angiogenesis by inhibiting proliferation and migration of endothelial cells and down-regulating the expression of VEGF and adhesion molecules ICAM-1.
Angiogenesis Inhibitors
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pharmacology
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Animals
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Cell Movement
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drug effects
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Cells, Cultured
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Chorioallantoic Membrane
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drug effects
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Curcumin
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analogs & derivatives
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pharmacology
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Humans
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Intercellular Adhesion Molecule-1
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genetics
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RNA, Messenger
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analysis
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Vascular Cell Adhesion Molecule-1
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genetics
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Vascular Endothelial Growth Factor A
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genetics
3.A new strategy for Chinese medicine processing technologies: coupled with individuation processed and cybernetics.
Ding-kun ZHANG ; Ming YANG ; Xue HAN ; Jun-zhi LIN ; Jia-bo WANG ; Xiao-he XIAO
China Journal of Chinese Materia Medica 2015;40(16):3323-3326
The stable and controllable quality of decoction pieces is an important factor to ensure the efficacy of clinical medicine. Considering the dilemma that the existing standardization of processing mode cannot effectively eliminate the variability of quality raw ingredients, and ensure the stability between different batches, we first propose a new strategy for Chinese medicine processing technologies that coupled with individuation processed and cybernetics. In order to explain this thinking, an individual study case about different grades aconite is provided. We hope this strategy could better serve for clinical medicine, and promote the inheritance and innovation of Chinese medicine processing skills and theories.
Chemistry, Pharmaceutical
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methods
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standards
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Cybernetics
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standards
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Drug Therapy
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standards
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Drugs, Chinese Herbal
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chemistry
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standards
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toxicity
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Humans
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Quality Control
4.Nutrition Factors Influence the Production of Insecticidal Crystal Proteins Cry1 and Cry2 from Bacillus thuringiensis 4.0718
Fei LIU ; Li-Qiu XIA ; Xue-Zhi DING ; Yong YI ; Xiang-Tao MO ; Wei WEI ;
Microbiology 2008;0(08):-
In order to increase the production of insecticidal crystal proteins Cry1 and Cry2, firstly, Plack-ett-Burman design was applied to evaluate the effectiveness of the related nutrition factors; it was found that the soybean powder and MnSO4?H2O were significant factors for Cry1 production, but the yield of Cry2 wasn’t effected remarkably in such medium. Then the steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration of the soybean powder and MnSO4?H2O was 11.5 and 0.02 g/L, obtained by response surface methodology (RSM). The final yields of Cry1 and Cry2 was 0.32 mg/mL and 0.11 mg/mL, increasing twice more than that in the medium optimized before. The median lethal concentration (LC50) of optimal medium was 1.09 ?L/mL. The toxicity to Heli-coverpa armigera was significantly enhanced than the old one.
6.Three cyclic dipeptides from the root of Psammosilene tunicoides.
Zhong-tao DING ; Zhi-juan BAO ; Xue-qiong YANG ; Ming JIANG
China Journal of Chinese Materia Medica 2003;28(4):337-339
OBJECTIVE[corrected] To study the cyclic peptides from Psammosilene tunicoides.
METHODThe constituents were separated and purified with chromatographic methods, identified by NMR, MS, UV and IR.
RESULTThree cyclic dipeptides: cyclo(-Pro-Val-) (1), cyclo(-Pro-Ala-) (2) and cyclo(-Pro-Pro-) (3), were isolated and identified.
CONCLUSIONCompound 1 and 2 are new natural products, compound 3 was isolated for the first time from Psammosilene tunicoides.
Caryophyllaceae ; chemistry ; Dipeptides ; chemistry ; isolation & purification ; Molecular Structure ; Peptides, Cyclic ; chemistry ; isolation & purification ; Plant Roots ; chemistry ; Plants, Medicinal ; chemistry
7.Applylication of new type combined fragments: nrDNA ITS+ nad 1-intron 2 for identification of Dendrobium species of Fengdous.
Li-xia GENG ; Rui ZHENG ; Jie REN ; Zhi-tao NIU ; Yu-long SUN ; Qing-yun XUE ; Wei LIU ; Xiao-yu DING
Acta Pharmaceutica Sinica 2015;50(8):1060-1067
In this study, 17 kinds of Dendrobium species of Fengdous including 39 individuals were collected from 4 provinces. Mitochondrial gene sequences co I, nad 5, nad 1-intron 2 and chloroplast gene sequences rbcL, matK amd psbA-trnH were amplified from these materials, as well as nrDNA ITS. Furthermore, suitable sequences for identification of Dendrobium species of Fengdous were screened by K-2-P and P-distance. The results showed that during the mentioned 7 sequences, nrDNA ITS, nad 1-intron 2 and psbA-trnH which had a high degree of variability could be used to identify Dendrobium species of Fengdous. However, single fragment could not be used to distinguish D. moniliforme and D. huoshanense. Moreover, compared to other combined fragments, new type combined fragments nrDNA ITS+nad 1-intron 2 was more effective in identifying the original plants of Dendrobium species and could be used to identify D. huoshanense and D. moniliforme. Besides, according to the UPGMA tree constructed with nrDNA ITS+nad 1-intron 2, 3 inspected Dendrobium plants were identified as D. huoshanense, D. moniliforme and D. officinale, respectively. This study identified Dendrobium species of Fengdous by combined fragments nrDNA ITS+nad 1-intron 2 for the first time, which provided a more effective basis for identification of Dendrobium species. And this study will be helpful for regulating the market of Fengdous.
DNA, Plant
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genetics
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DNA, Ribosomal Spacer
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genetics
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Dendrobium
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classification
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genetics
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Genes, Chloroplast
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Genes, Plant
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Introns
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Plants, Medicinal
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classification
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genetics
8.Evaluation of genetic diversity and population structure of Bletilla striata based on SRAP markers.
Yu-long SUN ; Bei-wei HOU ; Li-xia GENG ; Zhi-tao NIU ; Wen-jin YAN ; Qing-yun XUE ; Xiao-yu DING
Acta Pharmaceutica Sinica 2016;51(1):147-152
Bletilla striata has been used as traditional Chinese medicine for several centuries. In recent years, the quality and quantity of wild B. striata plants have declined sharply due to habitat deterioration and human over-exploitation. Therefore, it is of great urgency to evaluate and protect B. striata wild plant resource. In this study, sequence-related amplified polymorphism (SRAP) markers were applied to assess the level and pattern of genetic diversity in twelve populations of B. striata. The results showed a high level of genetic diversity (PPB = 90.48%, H = 0.349 4, I = 0.509 6) and moderate genetic differentiation among populations (G(st) = 0.260 9). Based on the unweighted pair-group method with arithmetic average (UPGMA), twelve populations gathered in three clusters. The cluster 1 included four populations. There are Nanjing, Zhenjiang, Xuancheng and Hangzhou. The seven populations which come from Hubei Province, Hunan Province, Jiangxi Province and Guizhou Province belonged to the cluster 2. The cluster 3 only contained Wenshan population. Moreover, Mantel test revealed significant positive correlation between genetic distances and geographic distances (r = 0.632 9; P < 0.000 1). According to the results, we proposed a series of conservation consideration for B. striata.
China
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Genetic Markers
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Genetic Variation
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Genetics, Population
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Orchidaceae
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genetics
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Phylogeny
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Plants, Medicinal
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genetics
9.Quality control methods and requirements for recombinant human lymphocyte function associated antigen 3 IgG1 fusion protein (rhLFA3-IgG1).
Kai GAO ; Chun-mei HAN ; You-xue DING ; Sheng HOU ; Chun-ming RAO ; Jun-zhi WANG
Acta Pharmaceutica Sinica 2007;42(7):762-767
To establish methods and requirements for quality control of rhLFA3-IgG1, biological potency of rhLFA3-IgG1 was determined by CD2 molecule competitive binding assay on Jurkat cell surface. Purity of rhLFA3-IgG1 was analyzed by SEC-HPLC and IEC-HPLC. Peptide mapping was preformed by tryptic digestion and RP-HPLC after sample reduced and carboxymethylation by DTT and indoacetic acid, respectively. CHO host cell protein and Protein A residual were detected by ELISA separately. The quality control methods and requirements, such as biological potency, the physical-chemical characteristic of rhLFA3-IgG1 had been established. The methods and requirements for quality control of rhLFA3-IgG1 showed advantages of assuring the products safety and efficacy, which can be used for routine quality control of rhLFA3-IgG1.
Binding, Competitive
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Biotechnology
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methods
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CD2 Antigens
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metabolism
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CD58 Antigens
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biosynthesis
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chemistry
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Chromatography, High Pressure Liquid
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Humans
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Immunoglobulin G
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biosynthesis
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chemistry
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Jurkat Cells
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Molecular Weight
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Peptide Mapping
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Quality Control
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Recombinant Fusion Proteins
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biosynthesis
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chemistry
10.A clinical study on manshuailing oral liquid in treating elder patients with congestive heart failure of type heart and kidney yang deficiency.
Ding-you YANG ; Xing-li WU ; Hao XU ; Xue-zhong DUAN ; Shi-wen WANG ; Zhi-zheng LU
China Journal of Chinese Materia Medica 2003;28(11):1091-1093
OBJECTIVETo investigate the clinical effect of manshuailing oral liquid on patients with congestive heart failure of type heart and kidney Yang deficiency.
METHOD90 patients of heart failure were randomly divided into 2 groups. 45 cases in the routine treatment group (RT) received general therapy including diuretics and digitalis, and 45 cases in the Chinese herb medicine group (CH) were treated basically with the above medicine, with additional manshuailing oral liquid. The clinical effect was summarized 6 weeks after treatment.
RESULTTotal effect rate was 82.2% and 62.2% in CH and RTgroup respectively. Compared with pretreatment, heart function including stroke volume (SV), stroke volume index (SVI), cardiac index (CI), shorten rate of left ventricular short axe (deltaD%), distance of inter-ventricular septal to mitral valve (EPSS) were all improved significantly in both groups (P < 0.05 or P < 0.01), and with even better effects in the CH group than the RT group (P < 0.05 or P < 0.01), except the SV.
CONCLUSIONManshuailing oral liquid can alleviate clinical symptom, decreased EPSS, increase deltaD% and improve heart function.
Administration, Oral ; Adult ; Aged ; Cardiotonic Agents ; therapeutic use ; Combined Modality Therapy ; Diagnosis, Differential ; Digoxin ; therapeutic use ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; therapeutic use ; Female ; Heart Failure ; drug therapy ; physiopathology ; Heart Function Tests ; Humans ; Hydrochlorothiazide ; therapeutic use ; Isosorbide Dinitrate ; therapeutic use ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Phytotherapy ; Plants, Medicinal ; chemistry ; Sodium Chloride Symporter Inhibitors ; therapeutic use ; Vasodilator Agents ; therapeutic use ; Yang Deficiency ; drug therapy