1.Treatment of fungal septicemia in a premature infant with caspofungin.
Xiao-chun DING ; Xue-ping ZHU ; Zhi-hui XIAO
Chinese Journal of Pediatrics 2008;46(6):479-discussion 480
2.Study on anti-angiogenesis effect of three curcumin pigments and expression of their relevant factors.
Yan-fen HUANG ; Xue-xin ZHU ; Zhi-shan DING ; Gui-yuan LV
China Journal of Chinese Materia Medica 2015;40(2):324-329
To study the in vitro anti-angiogenesis effect of three curcumin pigments (curcumin, demethoxycurcumin, bisdemethoxycurcumin). In the study, the inhibitory effect of the three curcumin pigments on proliferation of HUVEC cells induced by OX-LDL and the effect on migration of HUVEC cells were detected. The effect on neovascularization was observed by chorioallantoic membrane (CAM) test. The effect on cell adhesion factors ICAM-1 and VCAM-1 of HUVECs were tested by Real-time RT-PCR. It was found that the three curcumins could inhibit the proliferation of HUVEC cells induced by OX-LDL within the dosage range 4, 8, 16 mg x L(-1), with a dose-dependence. The proliferative effect of curcumins on HUVECs was greater than the other two derivatives (P < 0.01). All of the three curcumin pigments inhibited the migration of HUVEC cells and the angiogenesis of chick chorioallantoic membrane (CAM). The migration inhibition rate of curcumins at middle and high concentrations was greater than the other two (P < 0.01). All of the three curcumin could down-regulate the expression of VEGF and ICAM-1, and curcumins showed more obvious effect in down-regulating VEGF than demethoxycurcumin and bisdemethoxycurcumin(P < 0.01); Bisdemethoxycurcumin showed the most significant effect in down-regulating ICAM-1 (P < 0.01). All of the three showed no remarkable effect on expression of VCAM-1, and only bisdemethoxycurcumin showed the down-regulating effect (P < 0.05). According to the findings, all of the three curcumin pigments could resist angiogenesis by inhibiting proliferation and migration of endothelial cells and down-regulating the expression of VEGF and adhesion molecules ICAM-1.
Angiogenesis Inhibitors
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pharmacology
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Animals
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Cell Movement
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drug effects
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Cells, Cultured
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Chorioallantoic Membrane
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drug effects
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Curcumin
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analogs & derivatives
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pharmacology
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Humans
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Intercellular Adhesion Molecule-1
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genetics
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RNA, Messenger
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analysis
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Vascular Cell Adhesion Molecule-1
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genetics
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Vascular Endothelial Growth Factor A
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genetics
3.A new strategy for Chinese medicine processing technologies: coupled with individuation processed and cybernetics.
Ding-kun ZHANG ; Ming YANG ; Xue HAN ; Jun-zhi LIN ; Jia-bo WANG ; Xiao-he XIAO
China Journal of Chinese Materia Medica 2015;40(16):3323-3326
The stable and controllable quality of decoction pieces is an important factor to ensure the efficacy of clinical medicine. Considering the dilemma that the existing standardization of processing mode cannot effectively eliminate the variability of quality raw ingredients, and ensure the stability between different batches, we first propose a new strategy for Chinese medicine processing technologies that coupled with individuation processed and cybernetics. In order to explain this thinking, an individual study case about different grades aconite is provided. We hope this strategy could better serve for clinical medicine, and promote the inheritance and innovation of Chinese medicine processing skills and theories.
Chemistry, Pharmaceutical
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methods
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standards
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Cybernetics
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standards
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Drug Therapy
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standards
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Drugs, Chinese Herbal
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chemistry
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standards
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toxicity
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Humans
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Quality Control
4.Nutrition Factors Influence the Production of Insecticidal Crystal Proteins Cry1 and Cry2 from Bacillus thuringiensis 4.0718
Fei LIU ; Li-Qiu XIA ; Xue-Zhi DING ; Yong YI ; Xiang-Tao MO ; Wei WEI ;
Microbiology 2008;0(08):-
In order to increase the production of insecticidal crystal proteins Cry1 and Cry2, firstly, Plack-ett-Burman design was applied to evaluate the effectiveness of the related nutrition factors; it was found that the soybean powder and MnSO4?H2O were significant factors for Cry1 production, but the yield of Cry2 wasn’t effected remarkably in such medium. Then the steepest ascent experiment was adopted to approach the optimal region of the medium composition. Lastly, the optimal concentration of the soybean powder and MnSO4?H2O was 11.5 and 0.02 g/L, obtained by response surface methodology (RSM). The final yields of Cry1 and Cry2 was 0.32 mg/mL and 0.11 mg/mL, increasing twice more than that in the medium optimized before. The median lethal concentration (LC50) of optimal medium was 1.09 ?L/mL. The toxicity to Heli-coverpa armigera was significantly enhanced than the old one.
6.Applylication of new type combined fragments: nrDNA ITS+ nad 1-intron 2 for identification of Dendrobium species of Fengdous.
Li-xia GENG ; Rui ZHENG ; Jie REN ; Zhi-tao NIU ; Yu-long SUN ; Qing-yun XUE ; Wei LIU ; Xiao-yu DING
Acta Pharmaceutica Sinica 2015;50(8):1060-1067
In this study, 17 kinds of Dendrobium species of Fengdous including 39 individuals were collected from 4 provinces. Mitochondrial gene sequences co I, nad 5, nad 1-intron 2 and chloroplast gene sequences rbcL, matK amd psbA-trnH were amplified from these materials, as well as nrDNA ITS. Furthermore, suitable sequences for identification of Dendrobium species of Fengdous were screened by K-2-P and P-distance. The results showed that during the mentioned 7 sequences, nrDNA ITS, nad 1-intron 2 and psbA-trnH which had a high degree of variability could be used to identify Dendrobium species of Fengdous. However, single fragment could not be used to distinguish D. moniliforme and D. huoshanense. Moreover, compared to other combined fragments, new type combined fragments nrDNA ITS+nad 1-intron 2 was more effective in identifying the original plants of Dendrobium species and could be used to identify D. huoshanense and D. moniliforme. Besides, according to the UPGMA tree constructed with nrDNA ITS+nad 1-intron 2, 3 inspected Dendrobium plants were identified as D. huoshanense, D. moniliforme and D. officinale, respectively. This study identified Dendrobium species of Fengdous by combined fragments nrDNA ITS+nad 1-intron 2 for the first time, which provided a more effective basis for identification of Dendrobium species. And this study will be helpful for regulating the market of Fengdous.
DNA, Plant
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genetics
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DNA, Ribosomal Spacer
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genetics
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Dendrobium
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classification
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genetics
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Genes, Chloroplast
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Genes, Plant
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Introns
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Plants, Medicinal
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classification
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genetics
7.Quality control methods and requirements for recombinant human lymphocyte function associated antigen 3 IgG1 fusion protein (rhLFA3-IgG1).
Kai GAO ; Chun-mei HAN ; You-xue DING ; Sheng HOU ; Chun-ming RAO ; Jun-zhi WANG
Acta Pharmaceutica Sinica 2007;42(7):762-767
To establish methods and requirements for quality control of rhLFA3-IgG1, biological potency of rhLFA3-IgG1 was determined by CD2 molecule competitive binding assay on Jurkat cell surface. Purity of rhLFA3-IgG1 was analyzed by SEC-HPLC and IEC-HPLC. Peptide mapping was preformed by tryptic digestion and RP-HPLC after sample reduced and carboxymethylation by DTT and indoacetic acid, respectively. CHO host cell protein and Protein A residual were detected by ELISA separately. The quality control methods and requirements, such as biological potency, the physical-chemical characteristic of rhLFA3-IgG1 had been established. The methods and requirements for quality control of rhLFA3-IgG1 showed advantages of assuring the products safety and efficacy, which can be used for routine quality control of rhLFA3-IgG1.
Binding, Competitive
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Biotechnology
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methods
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CD2 Antigens
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metabolism
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CD58 Antigens
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biosynthesis
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chemistry
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Chromatography, High Pressure Liquid
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Humans
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Immunoglobulin G
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biosynthesis
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chemistry
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Jurkat Cells
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Molecular Weight
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Peptide Mapping
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Quality Control
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Recombinant Fusion Proteins
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biosynthesis
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chemistry
8.Effects of shenqifuxin oral liquid on the plasma kaliuretic peptide, the myocardial contractility and relaxation of left ventricle and the left ventricular remodeling in experimental rats with heart failure.
Lu MA ; Yan LEI ; Qiao XUE ; Shi-wen WANG ; Ding-you YANG ; Zhi-zheng LU
China Journal of Chinese Materia Medica 2003;28(7):656-660
OBJECTIVETo observe the effects of Shenqifuxin oral liquid(SQFXOL) on plasma kaliuretic peptide (KP), atrial natriuretic polypeptide(ANP), angiotension II (Ang II), endothelin(ET) and the left ventricular remodeling and the myocardial contractility and relaxation of left ventricle in experimental rats with heart failure(HF).
METHODThe SD rat model with HF was produced by constricting abdominal aorta. Hemodynamic parameters including maximum rate of intraventricular pressure rise (+dp/dtmax), left ventricular systolic pressure(LVSP), maximum velocity of contractile element shortening(Vmax), maximum rate of intraventricular pressure down(-dp/dtmax) and left ventricular end diastolic pressure(LVEDP) were measured by the method of the catheterization. Plasma concentrations of KP, ANP, Ang II and ET were determined by radioimmunoassays. The effects of treatment were evaluated by observing and comparing the changes of heart morphological structure, collagen element, heart weight/body weight ratio (HW/BW), left intraventricular area(LVA) and myocardial nuclei number (MNN) per square area.
RESULTIn high dose SQFXOL group, the LVSP, -dp/dtmax and Vmax were increased, while LVEDP was decreased, and plasma concentrations of KP, Ang II and ET were decreased. In comparision with those in model group, the difference was significant(P < 0.05 or P < 0.01). Though the +dp/dtmax and the level of ANP were decreased, the difference was insignificant(all P > 0.05). The collagen tissues around myocardial cells were reduced. HW/BW and LVA were lower, and MNN per square area was higher significantly (P < 0.05 or P < 0.01). The indices of +dp/dtmax in all of treatment groups and control group were not considerably different in comparison with those in model group. The levels of plasma ANP in middle dose group and low dose group were significantly lower than those in model group(all P < 0.01).
CONCLUSIONSQFXOL can reduce the plasma concentrations of KP, Ang II, ET, and ANP, improve the myocardial contractility and relaxation of left ventricular and inhibitate left ventricular remodeling in rats with HF.
Administration, Oral ; Angiotensin II ; blood ; Animals ; Astragalus membranaceus ; chemistry ; Atrial Natriuretic Factor ; blood ; Cardiotonic Agents ; administration & dosage ; pharmacology ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; pharmacology ; Endothelins ; blood ; Heart Failure ; blood ; physiopathology ; Male ; Myocardial Contraction ; drug effects ; Ophiopogon ; chemistry ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Protein Precursors ; blood ; Rats ; Rats, Sprague-Dawley ; Ventricular Function, Left ; Ventricular Remodeling ; drug effects
9.Nocardial infection after allogeneic hematopoietic stem cell transplantation.
Jing LIU ; Mei XUE ; Hong-Min YAN ; Zhi-Dong WANG ; Ling ZHU ; Li DING ; Heng-Xiang WANG
Journal of Experimental Hematology 2009;17(5):1339-1341
In order to explore the diagnosis and therapeutic effectiveness of nocardiosis after allogenic hematopoietic stem cell transplantation (allo-HSCT), the features of clinical manifestation, laboratory examination and response to TMP-SMZ treatment in two cases of nocardiosis after allo-HSCT were analyzed retrospectively. The result showed that the attack happened to 2 patients at day 15 and 170 after allo-HSCT respectively, displaying fever and chest pain. Chest CT scan indicated bilateral pulmonary tuberculous shadow. Nocardiosis was diagnosed by the culture of sputum, bronchoalveolar lavage (BAL) fluid and pus samples as well. Both of these cases exhibited good response to combined therapy containing TMP-SMZ for half a year. It is concluded that nocardiosis is a rare complication after allo-HSCT, in which pulmonary involvement is commonly observed. The culture of BAL fluid is helpful for its diagnosis and this disease might be sensitive to the treatment of TMP-SMZ-containing regimens.
Adolescent
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Female
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Hematopoietic Stem Cell Transplantation
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adverse effects
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Humans
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Male
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Middle Aged
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Nocardia Infections
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etiology
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Retrospective Studies
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Transplantation, Homologous
10.A clinical study on manshuailing oral liquid in treating elder patients with congestive heart failure of type heart and kidney yang deficiency.
Ding-you YANG ; Xing-li WU ; Hao XU ; Xue-zhong DUAN ; Shi-wen WANG ; Zhi-zheng LU
China Journal of Chinese Materia Medica 2003;28(11):1091-1093
OBJECTIVETo investigate the clinical effect of manshuailing oral liquid on patients with congestive heart failure of type heart and kidney Yang deficiency.
METHOD90 patients of heart failure were randomly divided into 2 groups. 45 cases in the routine treatment group (RT) received general therapy including diuretics and digitalis, and 45 cases in the Chinese herb medicine group (CH) were treated basically with the above medicine, with additional manshuailing oral liquid. The clinical effect was summarized 6 weeks after treatment.
RESULTTotal effect rate was 82.2% and 62.2% in CH and RTgroup respectively. Compared with pretreatment, heart function including stroke volume (SV), stroke volume index (SVI), cardiac index (CI), shorten rate of left ventricular short axe (deltaD%), distance of inter-ventricular septal to mitral valve (EPSS) were all improved significantly in both groups (P < 0.05 or P < 0.01), and with even better effects in the CH group than the RT group (P < 0.05 or P < 0.01), except the SV.
CONCLUSIONManshuailing oral liquid can alleviate clinical symptom, decreased EPSS, increase deltaD% and improve heart function.
Administration, Oral ; Adult ; Aged ; Cardiotonic Agents ; therapeutic use ; Combined Modality Therapy ; Diagnosis, Differential ; Digoxin ; therapeutic use ; Drug Combinations ; Drugs, Chinese Herbal ; administration & dosage ; isolation & purification ; therapeutic use ; Female ; Heart Failure ; drug therapy ; physiopathology ; Heart Function Tests ; Humans ; Hydrochlorothiazide ; therapeutic use ; Isosorbide Dinitrate ; therapeutic use ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Phytotherapy ; Plants, Medicinal ; chemistry ; Sodium Chloride Symporter Inhibitors ; therapeutic use ; Vasodilator Agents ; therapeutic use ; Yang Deficiency ; drug therapy