2.A Study on Cultivation of Acid-tolerant Methanogenic Granular Sludge under Low pH Values and its Characteristics
Jian-E ZUO ; Xue-Feng LING ; Xia-Sheng GU ;
Microbiology 1992;0(05):-
Two lab-scale expanded granular sludge bed reactors were operated using granular sludge and river sediments as seed sludge respectively, through gradually lowering down the inner pH values, acid-tolerant methanogenic granular sludge with good methanogenic activity were acquired and formed. Two EGSB reactors could be operated steadily under the condition of pH 5.8~6.2, and their volumetric loading rates were about 5.5~7.5 kg COD/(m3/d), the COD removal efficiencies were about 90%. The granular sludges taken from two reactors could maintain relatively higher methanogenic activity under low pH, and the relative activities of the granular could be 51.78% and 55.6% of the value with the condition of pH 7.0 when pH was 5.5. Studies on the diameter distribution, settling velocities, concentrations of different metal elements and microbiological characteristics of these acid-tolerant granular sludges were also conducted.
3.Biological characteristics and hematopoietic support of bone marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus
Hua-Yong ZHANG ; Xue-Bing FENG ; Ling-Yun SUN ;
Chinese Journal of Rheumatology 2000;0(06):-
Objective To explore the biological characteristics and karyotype of bone marrow-derived mesenchymal stem cells(MSCs)in patients with systemic lupus erythematosus(SLE)and hematopoietic sup- port of MSCs.Methods MSCs were isolated from bone marrow of 11 SLE patients and 6 healthy controls by density centrifugation and adhesive culture in vitro.The surface markers were detected by flow cytometry (FCM).The morphological changes of MSCs were observed in primary and passage cultures.The growth curves were assayed.The karyotype of MSCs was detected by blocking cellular mitosis with colchicines.The MSCs from SLE patients and healthy controls were infused to ICR mice after high-dose chemotherapy.The changes of peripheral blood counts of the mice were recorded.Results Approximately(6~9)?10~9 MSCs from SLE were obtained after 5 passages and their growth was slower than normal controls(P<0.01).Both groups were positive for CD29,CD44 and CD105,and negative for CD14,CD34,CD45 and HLA-DR.MSCs from SLE had a normal karyotype.MSCs infusions of the two groups were accompanied by no adverse event and the recovery of white blood cell,hemoglobin and platelet count was quicker when compared with the controls(P<0.05).Conclusion MSCs from SLE have demonstrated abnormalities in expansion in vitro.MSCs from SLE have a normal karyotype.Ex vivo MSCs infusion from SLE patients can support hematopoiesis as normal MSCs.
4.Comparison of Stable Expressions of Foreign Genes Driven by Different Promoters in Transgenic Dunaliella salina
Jie LI ; Dong-Jing QU ; Ling-Ling LIU ; Shu-Yeng FENG ; Le-Xun XUE ;
China Biotechnology 2006;0(03):-
The purpose was to compare the difference between transgene expressions driven by homologous duplicated carbonic anhydrase (DCA) promoter and foreign CaMV35S promoter in the unicellular green alga, Dunaliella salina(D.salina).The CaMV35S promoter-bar construct and DCA promoter-bar construct into D.salina by a Backon 2000 electroporation system were introduced. After the repeated selections with the phosphinothricin (PPT) of 3mg/L, 3 PPT-resistant phenotype transformants were isolated from the CaMV-bar and DCA-bar pools of transformants of D. salina, respectively. The results of PCR and sequencing showed that bar genes were stably integrated into the genome of D.salina, and Southern bolts showed the number of transgene copy had no significant difference between both promoters. Semi-quantitive RT-PCR indicated that the mRNA levels of bar gene were higher in DCA-bar transformants than the CaMV-bar transformants, and could be increased under the induction of high salt in DCA-bar transformants but not in the CaMV-bar transformants. Analysis of growth rate of transformants showed DCA-bar transformants achieved the log stage faster than the CaMV-bar transformants. It is concluded that the homologous promoters have more advantages than the foreign promoters in the transgenic D.salina.
5.Comparative study on identification of Clematidis Radix et Rhizoma from different origins based on microstructure and NIRS method.
Ya XU ; Hui LI ; Xue-feng FENG ; Yu-ling LIU ; Xiao-li ZHANG
China Journal of Chinese Materia Medica 2015;40(7):1278-1282
Microscopic identification and NIRS methods were applied to identify Clematidis Radix et Rhizoma of two different origins. The results showed that both methods could identify the samples. NIRS could identify the two samples nondestructively, and provides a basis for establishment of a standard herbs radix clematidis NIRS fingerprint in the future.
China
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Clematis
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chemistry
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classification
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Drugs, Chinese Herbal
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analysis
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chemistry
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Rhizome
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chemistry
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Spectroscopy, Near-Infrared
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methods
6.Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation
Jiangang FENG ; Shuang LI ; Jiangbin FENG ; Ling GAO ; Xue LU ; Deqing CHEN ; Qingjie LIU
Chinese Journal of Radiological Medicine and Protection 2015;35(4):245-247,302
Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation.Methods AHH-1 cells was exposed to different doses(0,1,3,5,8,10,15 and 18 Gy)of 60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4,8,12,24,48 and 72 h after irradiation.Results Within the range of applied doses,the level of S100A4 gene expression was upregulated with a good dose-response (R2 =0.79-0.93,P < 0.05) and had obvious difference at different time points (F =8.91,P < 0.01).Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation,and hence is applicable as a dosimeter.
7.Relationship of Chinese medicine syndrome with endothelium-dependent vasodilatation function and carotid intima-media thickness in patients with hypertensive disease.
Xue-Ling LIU ; Ai-Ling WEI ; Feng LUO
Chinese Journal of Integrated Traditional and Western Medicine 2009;29(9):833-835
OBJECTIVETo explore the relationship of Chinese medicine (CM) syndrome with carotid intima-media thickness (CIMT) and endothelium-dependent vasodilatation function (EDVF) in patients with hypertensive disease (HD) for providing an objective basis of syndrome differentiation in HD patients.
METHODSColor Doppler's ultrasound was used to measure the endothelium-dependent flow-mediated dilation (FMD) of brachial artery and carotid intima-media thickness (CIMT) in 60 HD patients (the HD group) and 30 normal controls (the control group). And the relationship of the outcomes with Chinese medicine syndrome types in patients was analyzed statistically.
RESULTSFMD was lower and CIMT was higher in HD patients of all syndrome types than those in the control group respectively (P<0.01). Comparison between patients of different syndrome types showed that FMD was higher in patients of Gan-fire exuberance type and yin-deficiency and yang-hyperaction type than in those of both yin-yang deficiency type and phlegm-dampness stagnancy type (P<0.01, P<0.05), while CIMT in patients of Gan-fire exuberance type was the lowest in all types, and that in yin-deficiency and yang-hyperaction type was lower than in yin-yang deficiency type (P<0.01).
CONCLUSIONCIMT and FMD may be used as a reference index for CM syndrome differentiation in HD patients.
Adult ; Aged ; Carotid Intima-Media Thickness ; Case-Control Studies ; Endothelium-Dependent Relaxing Factors ; Female ; Humans ; Hypertension ; diagnosis ; diagnostic imaging ; physiopathology ; Male ; Medicine, Chinese Traditional ; methods ; Middle Aged ; Tunica Intima ; diagnostic imaging ; Tunica Media ; diagnostic imaging
8.Some experiences in the treatment for trichloroethylene-induced medicamentosa like dermatitis.
Feng-ling ZHAO ; Xiao-li WANG ; Xue-chun XU ; Chun-he WEN
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(3):229-230
Adolescent
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Adult
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Dermatitis, Occupational
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etiology
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therapy
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Drug Eruptions
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etiology
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therapy
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Female
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Humans
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Male
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Methylprednisolone
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therapeutic use
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Trichloroethylene
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adverse effects
9.Genotype analysis of Mycoplasma pneumoniae during 2010-2012 in children from Beijing
Hongmei SUN ; Guanhua XUE ; Chao YAN ; Yanling FENG ; Liqiong WANG ; Hanqing ZHAO ; Shaoli LI ; Ling CAO
Chinese Journal of Microbiology and Immunology 2012;(11):939-943
Objective To inspect the source of an outbreak with Mycoplasma pneumoniae (Mp).Methods We carried out real-time PCR to analyze specimens collected from pediatric patients in Beijing during January 2010 to May 2012,diagnosed as pneumonia or a respiratory infection according to clinical symptoms.These positive samples were analyzed by the M-P typing system(M:multiple-locus variable-number tandem-repeat analysis,MLVA; P:P1-restriction fragment length polymorphism analysis,P1-RFLP).Results Sixty-nine specimens were tested positive to Mp by the real-time PCR in 446 specimens from pediatric patients.The infection rate was 11.69%,15.56% and 20.00% respectively in 2010,2011 and the first half of 2012.According to the M-P system,11 distinct genotypes were identified from 69 positive specimens,M43562P1 and M53562P1 were the two main genotypes that showed an increasing trend from 2010 to 2011,and M33562P1 and M63562P1 showed an increasing trend from 2011 to 2012 in China.Conclusion During this international Mp epidemic,the infection rate of Mp was also increase in Beijing in 2011,and M43562P1 and M53562P1 were the two main genotypes.Among them,M43562 were consistent with pop genotypes in Europe,and M53562 were consistent with pop genotype in Israel.The M-P system would be valuable to monitor the epidemic of Mp in different countries in the world.
10.Emergency reparation and reconstruction for complicated tissue defects in hand
Yi-Min CHAI ; Bing-Fang ZENG ; Qing-Ling KANG ; Jian-Feng XUE ; Ji SHEN ; Jia JIANG ;
Chinese Journal of Microsurgery 2006;0(05):-
Objective To explore the operative methods and clinical outcomes in emergency or sube- mergency repair for the complicated tissue defects in hand in first stage applying microsurgical technique. Methods From Jan.,2000 to Aug.,2005,49 emergency cases of complicated hand tissue defects were re- paired in the first stage with replantation,reconstruction,free flaps,combined finger reconstruction and flap transplantation,including 21 cases in mini tissue mass replantation or reconstruction,15 cases in replantation combined with free flap transplantation,8 cases in replantation and reconstruction combined with free flap transplantation,5 cases in combined multiple digits reconstruction with free flap transplantation.The free flap transplantation included the anterolateral femoral flap,the latissimus dorsi myocutaneous flap,the dorsalis pe- dis flap,the media pedis flap and the instep flap.Results All the flaps,the replanted and reconstruceted finger survived uneventfully except for one replanted finger necrosis.45 cases healed in the first stage and the other 4 cases healed in the second stage.During a follow-up from 6 months to 3 years postoperatively,a satis- factory appearance and function of the reconstructed hand was achieved.The excellent and good rate was 85.7% assessed with provisional functional assessment criterion for upper limbs issued by Chinese Society of Hand Surgery.Conclusion The emergency or subemergency repair for the complicated tissue defects in hand has the advantage of short-term treatment and desirable functional outcome.The emergency replantation and reconstruction combined with various flaps or tissue mass can be applied to repair tissue defect in hand in the first stage according to the position and area of the defect along with the technique level of the surgeon, having been proved to achieve desirable clinical outcomes.And the key points leading to a successful operation is the correct treatment for the raw surface of the defects,suitable choice for various flaps,logical design of combination pattern and prevention and timely treatment for vessel crisis.