Objective To investigate the mechanism of the fluorouracil(FU)drug resistance and to prove deeply the role of serum dihydropyrimidine dehydrogenase(DPD)activity in patients with gastric cancer resistance to FU with the level of cell culture. Methods The peripheral blood was collected from 45 patients with advanced gastric cancer before chemotherapy to detect the activity of DPD by high performance liquid chromatography(HPLC).The patients were divided into two groups(high activity group and low activity group)according to the results. The FU drug inhibitory rate of gastric cancer cell cultured in vitro were tested with MTT to compare the effects of the two groups. Result The FU drug inhibitory rate of gastric cancer cell cultured in vitro was obviously lower in high activity group than that in low activity group,(47.6±±4.0)%,(53.7±8.0)%respectively. Conclusion The activity of serum DPD may be simple and convenient indicators in predicting the resistance to FU.

Nitric oxide (NO) is the most important known endogenous vasodilator factor. The competitive inhibitor asymmetric dimethylarginine (ADMA) of endothelial nitric oxide synthase (NOS) inhibits NO synthesis, which makes NO/NOS pathway to be obstructed and the reduction of NO synthesis. The studies in recent years have suggested that ADMA is as-sociated with the occurrence and development of diabetes with cerebrovascular disease. It has provided new insights into further clarifying the mechanism of the disease.

Objective To investigate the analgesic efficacy of ultrasound guided transversus abdominis plane block and to compare the efficacy of posterior approach and medial approach of ultrasound guided transversus abdominis plane block (TAPB) in providing postoperative analgesia after children laparoscopic groin surgery.Methods Eligible children 2 ～ 4 years old (n =60),American society of anesthesiologists (ASA) I,undergoing elective laparoscopic unilateral groin surgery were randomly assigned into posterior approach group (Group P),medial approach group (Group M) and control group (Group C).Many parameters were measured and recorded during the study,including funk sedation scores,intraoperative circulation monitoring at time points of T1 (before induction),T2 (skin incision) and T3 (skin closure),length of surgery,the time from post anesthesia care unit (PACU) arrival to the first obtainable pain score,length of PACU stay,modified-children's hospital of eastern ontario pain scale(m-CHEOPS),pediatric anesthesia emergence delirium (PAED) scores,number of children with sufentanil administered in PACU,number of people occurred nausea and vomiting,and satisfaction of parents.Results There were no statistically significant difference in general characteristics,basic emotion score,duration of surgery,the time from PACU arrival to first obtainable score,haemodynamic parameter at T1 and satisfaction of parents among groups P,M,and C.However,the m-CHEOPS scores at the time points of first obtainable,10 min,the number of children received sufentanil administration,PAED scores and haemodynamic parameter at T2 and T3 were significantly lower in groups P and M than in group C.There were no significant difference in parameters mentioned above between groups P and M.Conclusions Ultrasound guided transversus abdominis plane block could provide efficient and secure postoperative analgesia for children undergoing laparoscopic groin surgery.Both posterior approach and medial approach are ideal options.

Objective: To study the antioxidative and hepatoprotective activities of low molecular fucoidan oligosaccharides(LMFO) from Laminaria japonica in mice.Methods: Mice were pretreated with LMFO(50?100?150 mg/kg ig respectively, 10 days),and then 0.2 % CCl 4 10 ml/kg ig and D-GalN(600 mg/kg)+LPS(lipopolysaccharide,1 ?g/kg) ig respectively in two model groups to induce liver injury. Liver injury was assessed by quantifying activities of plasma GPT, SOD, GSH-Px and MDA content.Results: The increase of plasma GPT activity was significantly inhibited by LMFO in two liver injury models, suggesting that LMFO had good protective effect on the hepatocytes. LMFO had good antioxidative effect in mice with liver injury induced by CCl 4 and D-GalN+LPS as indicated by decreased MDA content and increased activities of plasma SOD and GSH-Px. Conclusion: LMFO is protective against CCl 4-induced and D-GalN+ LPS induced liver injury in mice and its effect may be due to its antioxidative activities in vivo.

Objective To investigate the application value of low dose high pitch dual source CT angiography technique in diagno-sis of acute aortic dissection.Methods 21 cases of critically ill patients with suspected aortic dissection underwent total aortic angiog-raphy without using ECG by adopting the second generation dual source CT Flash Technology (pitch 3 .2 ,1 0 0 kV and 1 0 0 mAs), using a lower contrast dose (60 mL).Results All subjects accepted as low as possible radiation doses and iodine intake;at the same time,accurate information including aortic intimal tear position,intimal flap,true and false lumen,and lesions involving the range vessel signs and anatomic relationship were received,2 cases of Stanford type A,type B 1 9 cases (2 cases of aortic rupture).Average scan time 2.6 s,average effective radiation dose cm 270 mGy·cm.Conclusion High quality images by using high pitch dual source CT low dose angiography with lower contrast dose provide safe,reliable,real-time screening method for critically ill patients with aor-tic dissection caused by systemic severe compound injury.

Air Pollutants ; analysis ; Humans ; Industry ; Lead ; analysis ; chemistry ; Spectrometry, Fluorescence ; methods ; Spectrophotometry, Atomic ; methods ; Workplace

Animals ; Humans ; Interleukin-1 ; physiology ; Mice ; Pulmonary Fibrosis ; physiopathology ; Rats ; Receptors, Interleukin-1 ; physiology

In the present study the bioavailability of selenium in Se-yeast was compared with that in sodium selenite to rats fed on low-Se diet from a keshan discaes area. The bioavailability of selenium in Se-yeast when estimated by heart, liver kidney and erythrocyte Se contents was significantly greater than that in selenite at wk 2 and 4 of supplement, indicating that selenium in Se-yeast is more cfficiently retained in tissues than selenite selenium. When the criterion used was either platelet or liver glutathione peroxidase (GSH_(?)) activity, the bioavailability was lower for selenium in Se-yeast than for in selenite at wk 2, but higher at wk 4, showing that selenium from both selenite and Se-yeast is clearly available for GSH_(?), synthesis, "although Se-yeast restores GSH_(?) activity more slowly. In addition, the effect of selenium in Se-yeast in maintaining cither Se levels in heart and erythrocyte or GSH_(?) activities in platelets and liver was superior to that of selenite after the selenium supplements were withdrawn.

Objective:To analyze and compare the volatile oil in the leaves of Juniperus formosana Hayata from Yuzhong and Lu-qu. Methods:The volatile oil from Juniperus formosana Hayata was extracted by steam distillation, and then identified by gas chroma-tography-mass spectrometry ( GC-MS) . Results:The average yield of the volatile oil from the leaves of Juniperus formosana Hayata from Yuzhong and Lugu was 0. 21% and 1. 45%(w/w), respectively. Between the two areas of the volatile oil from the leaves of Juniperas formsana, totally 40 peaks were separated from the volatile oil from Gansu with 38 ones (95%) were identified. Totally 51 peaks were separated from the volatile oil from Xizang with 47 ones (92%) were identified. The maln constituents in the former volatile oil wereα-pinene(44.92%),l-caryophyllene(9.23%),( -)-isoledene(6. 50%),a-caryophyllene(5.60%),myrcene(4.54%) and d-cadinene(3. 37%), and those in the latter were di-epi-cedrene(31. 87%),cyclohexene(15. 28%),γ-elemene(10. 05%), lanceol (5. 80%) and α-pinene(5. 79%). Conclusion:The constituents in the volatile oil in the leaves of Juniperus formosana Hayata from the two different regions are various, and the differences in the yield and compounds are notable. The method provides reference for the establishment of quality standard for Juniperus. formosana Hayata.