Objective To detect the expression of YB-1 and to research the relationship in the occurrence and development in the cervical squamous carcinoma(SCC) tissues.Methods Immunohistochemistry(IHC) envision was used to detect the expression of chronic cervicitis,CIN Ⅰ,CIN Ⅱ-Ⅲ and SCC.The relationship of YB-1 in the clinical pathological parameters of SCC were analyzed.Results YB-1 was mainly located in the nucleus in squamous cell,sometimes in the cytoplasm.The YB-1 protein did not expression in chronic cervicitis.In CIN Ⅰ,CIN Ⅱ-lⅢ and SCC,the positive expression had a gradual increasing trend.The expression of YB-1 was satistically significant in four groups (P＜0.05).The chronic cervicitis group,CIN Ⅰ groupCIN Ⅱ-Ⅲ group compared with the SCC group restivelly,the difference was statistically significant (P＜0.05).From spearman rank correlation analysis:the expression of YB-1 was positively correlated with the degree of cervical lesions (P＜ 0.05).In the cervical squamous carcinoma group,the expression of YB-1 was not associated with clinical pathological index of SCC patients(P＞0.05).Conclusion The change of the quantity of YB-1 protein is closely related to cervical squamous cell carcinoma.

Equisetin (EQST) belongs to polyketide (PKS)-nonribosomal peptide synthetase (NRPS) type compound with an inhibitory effect of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) enzyme activity. This study investigated anti-obesity effect and insulin resistance improvement effect of EQST on high-fat diet (HFD)-induced ob/ob mice model. EQST treatment effectively reduced the body weight gain, fat weight gain and blood lipid content of model mice. All animal experiments were approved by the Medical Ethics Committee of Capital Institute of Pediatrics. EQST alleviated adipose tissue expansion and hepatic ballooning degeneration of model mice, and also effectively controlled the blood glucose content after glucose load and insulin load, showed a significant improvement in obesity and insulin resistance. EQST inhibited adipogenic proteins fatty acid-binding protein 4 (FABP4) and peroxisome proliferators-activated receptor γ (PPARγ), and upregulated thermogenic protein (uncoupling protein 1, UCP1) through suppressing 11β-HSD1 protein expression. In addition, EQST widely upregulates mitochondrial respiratory metabolism related proteins in adipose tissue and may improve insulin resistance through phosphatidylinositol-3-kinase (PI3K) pathway. Therefore, EQST plays an anti-obesity role by promoting adipose tissue thermogenesis and improving insulin resistance, which may provide reliable clues for improving obesity and diabetes.

Adenoid hypertrophy is a disease that mostly occurs among children of 3-5 years old. It is caused by repeated inflammation and infection of nasopharynx and its adjoin parts, or the adenoid itself, which will finally leads to pathological hyperplasia of adenoid. With so much information we have acquired about this disease, its specific mechanism remains unknown. In recent years, some researches have indicated that adenoid hypertrophy may have something to do with extra-gastroesophageal reflux, in which pepsin plays a very important role, and pepsin will do a series of pathological damages to the upper airway as it reaches the upper respiratory tract. Based on relative domestic and foreign literature, this paper attempts to make a review about the relationship between gastroesophageal reflux and adenoid hypertrophy.
Adenoids ; pathology ; Child ; Gastroesophageal Reflux ; complications ; Humans ; Hypertrophy ; complications ; Nasopharynx ; pathology ; Pepsin A ; metabolism

Because the biology characteristic of HIV is extremely different from that of other microorganisms, HIV vaccine research has being faced with unprecedented difficulties and challenges. In the last 20 years, HIV vaccine research has been carried out mainly with two strategies, i.e., neutralizing antibody based and cellular immunization based; however, substantive breakthroughs haven't been achieved until now. Inducing effective neutralizing antibody is always an important strategy in traditional vaccine research, but this strategy is much less efficient in HIV vaccine research because of high variation and much subtypes of HIV. In recent years, the discovery of broadly neutralizing monoclonal antibodies and elucidation of their corresponding antigen epitopes have brought new hopes for the development of neutralizing antibody based HIV vaccine. Reviewing these advancements will be helpful for reconsidering HIV vaccine development with a better strategy.

China ; Humans ; Industry ; statistics & numerical data ; Lead ; blood ; Occupational Exposure

Animals ; Antineoplastic Agents ; therapeutic use ; Gene Silencing ; Genetic Therapy ; methods ; Humans ; MicroRNAs ; antagonists & inhibitors ; therapeutic use ; Neoplasms ; genetics ; therapy ; RNA Interference ; physiology ; RNA, Small Interfering ; antagonists & inhibitors ; therapeutic use ; RNA-Induced Silencing Complex ; metabolism

Drug-induced nephrotoxicity is very common in both new drug development and clinic practice. Various drugs can induce kidney injuries, including tubulointerstitial, glomerular and renal vascular disease. To investigate the mechanism of drug induced nephrotoxicity is important for risk reduction of new drug development, reasonable drug usage, early discovery and effective prevention/treatment of adverse effects in clinics.

0.05). Conclusion The rapid paper strip method could be applied as the rapid method for monitoring the fecal colifom group in surface water.

Objective To investigate the effect of formoononetin on endostatin ( ES ) , vascular endothelial growth factor ( VEGF ) , matrix metalloproteinases 2(MMP-2), basic fibroblast growth factor (bFGF) in hydrothorax and serum, and tumor biomarkers of patients with elderly advanced lung cancer.Methods 67 cases elder with advanced lung cancer were selected and randomly divided into two groups.33 cases in control group were treated with conventional therapy of NP chemotherapy regimen, 34 cases in experiment group were combined with formononetin.The changes of ES, VEGF, MMP-2, bFGF, tumor marker levels in hydrothorax and serum and life quality evaluation were compared.ResuIts Compared with control group, the contents of ES, VEGF, MMP-2, bFGF in hydrothorax and serum of experiment group significantly decreased (P<0.05); the carcino embryonie antigen (CEA), neuron specific enolase (NSE), cytokeratin-19-fragments (CYFRA21-1), and carbohydrate antigen 125 (CA125) in serum of experiment group significantly decreased ( P<0.05 ); the life quality evaluation improved better of experiment group (χ2 =4.96, P<0.05 ). ConcIusion Formononetin has better clinical effect in treatment of elderly patients with advanced lung cancer patients.It could effectively improve the quality of life, reduce ES, VEGF, MMP-2, bFGF and other indicators in hydrothorax and serum, which has the vital significance to the clinical therapy.

Objective To explore the effect of cisapride combined with polyethylene glycol electrolytic solution on preoperative bowel preparation for patients receiving colorectal cancer operation. Methods One hundred and fifty-two patients received colorectal cancer opera-tion in our hospital from October 2010 to October 2014 were selected. Those who were treated with cisapride combined with polyethylene gly-col electrolytic solution for bowel preparation before operation were assigned to oberservation group(n=83),those who were treated with poly-ethylene glycol electrolytic solution for bowel preparation before operation were assigned to control group(n=69). The side effect after medi-cation, electrolyte concentration and incidence of incision infection between groups after operation were collected and compared. Results The incidence of nausea and vomiting was 7. 23% in observation group,which was significantly lower than 18. 84% in control group (χ2 =4. 64,P=0. 03). The difference of Na+,K+ and Cl- concentration between groups were not significant (t=1. 83,P=0. 07;t=0. 42,P=0. 68;t=1. 86,P=0. 07). The body temperature,leukocyte count and incidence of incision infection after operation in observation group were significantly lower than those in control group (t=2. 68,P<0. 01;t=3. 29,P<0. 01;χ2 =4. 90,P=0. 03). Conclusion Cisapride com-bined with polyethylene glycol electrolytic solution before operation can significantly decrease the incidence of gastrointestinal side effect after medicaiton and incision infection after operation and it is beneficial to recovery for patients with colorectal cancer operation.