1.The expression and significance of YB-1 in cervical squamous carcinoma
Chongqing Medicine 2017;46(6):777-779
Objective To detect the expression of YB-1 and to research the relationship in the occurrence and development in the cervical squamous carcinoma(SCC) tissues.Methods Immunohistochemistry(IHC) envision was used to detect the expression of chronic cervicitis,CIN Ⅰ,CIN Ⅱ-Ⅲ and SCC.The relationship of YB-1 in the clinical pathological parameters of SCC were analyzed.Results YB-1 was mainly located in the nucleus in squamous cell,sometimes in the cytoplasm.The YB-1 protein did not expression in chronic cervicitis.In CIN Ⅰ,CIN Ⅱ-lⅢ and SCC,the positive expression had a gradual increasing trend.The expression of YB-1 was satistically significant in four groups (P<0.05).The chronic cervicitis group,CIN Ⅰ groupCIN Ⅱ-Ⅲ group compared with the SCC group restivelly,the difference was statistically significant (P<0.05).From spearman rank correlation analysis:the expression of YB-1 was positively correlated with the degree of cervical lesions (P< 0.05).In the cervical squamous carcinoma group,the expression of YB-1 was not associated with clinical pathological index of SCC patients(P>0.05).Conclusion The change of the quantity of YB-1 protein is closely related to cervical squamous cell carcinoma.
2.Anti-obesity effect of 11β -HSD1 inhibitor equisetin on ob/ob mice
Acta Pharmaceutica Sinica 2022;57(8):2399-2404
Equisetin (EQST) belongs to polyketide (PKS)-nonribosomal peptide synthetase (NRPS) type compound with an inhibitory effect of 11
3.An association between adenoid hypertrophy and exstra-gastroesophageal reflux disease.
Jianjun REN ; Yu ZHAO ; Xue REN
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2015;29(15):1406-1408
Adenoid hypertrophy is a disease that mostly occurs among children of 3-5 years old. It is caused by repeated inflammation and infection of nasopharynx and its adjoin parts, or the adenoid itself, which will finally leads to pathological hyperplasia of adenoid. With so much information we have acquired about this disease, its specific mechanism remains unknown. In recent years, some researches have indicated that adenoid hypertrophy may have something to do with extra-gastroesophageal reflux, in which pepsin plays a very important role, and pepsin will do a series of pathological damages to the upper airway as it reaches the upper respiratory tract. Based on relative domestic and foreign literature, this paper attempts to make a review about the relationship between gastroesophageal reflux and adenoid hypertrophy.
Adenoids
;
pathology
;
Child
;
Gastroesophageal Reflux
;
complications
;
Humans
;
Hypertrophy
;
complications
;
Nasopharynx
;
pathology
;
Pepsin A
;
metabolism
4.Development of Neutralizing Antibody Based HIV/AIDS Vaccine
Xue-Ren LI ; Li RUAN ;
China Biotechnology 2006;0(08):-
Because the biology characteristic of HIV is extremely different from that of other microorganisms, HIV vaccine research has being faced with unprecedented difficulties and challenges. In the last 20 years, HIV vaccine research has been carried out mainly with two strategies, i.e., neutralizing antibody based and cellular immunization based; however, substantive breakthroughs haven't been achieved until now. Inducing effective neutralizing antibody is always an important strategy in traditional vaccine research, but this strategy is much less efficient in HIV vaccine research because of high variation and much subtypes of HIV. In recent years, the discovery of broadly neutralizing monoclonal antibodies and elucidation of their corresponding antigen epitopes have brought new hopes for the development of neutralizing antibody based HIV vaccine. Reviewing these advancements will be helpful for reconsidering HIV vaccine development with a better strategy.
6.Progress of the study of mechanism on drug induced kidney injury.
Xiang XUE ; Likun GONG ; Jin REN
Acta Pharmaceutica Sinica 2010;45(10):1199-204
Drug-induced nephrotoxicity is very common in both new drug development and clinic practice. Various drugs can induce kidney injuries, including tubulointerstitial, glomerular and renal vascular disease. To investigate the mechanism of drug induced nephrotoxicity is important for risk reduction of new drug development, reasonable drug usage, early discovery and effective prevention/treatment of adverse effects in clinics.
7.Advances in research on RNA interference-related drugs for cancer treatment.
Chinese Journal of Oncology 2012;34(11):801-804
Animals
;
Antineoplastic Agents
;
therapeutic use
;
Gene Silencing
;
Genetic Therapy
;
methods
;
Humans
;
MicroRNAs
;
antagonists & inhibitors
;
therapeutic use
;
Neoplasms
;
genetics
;
therapy
;
RNA Interference
;
physiology
;
RNA, Small Interfering
;
antagonists & inhibitors
;
therapeutic use
;
RNA-Induced Silencing Complex
;
metabolism
8.Clinical application of domestic rapamycin-eluting stents in coronary heart disease patients
Yuzeng XUE ; Hang GAO ; Haizhou REN
Chinese Journal of Interventional Cardiology 2003;0(06):-
Objective To evaluate the efficiency and safety of domestic rapamycin-eluting stent(Firebire stent) in the treatment of coronary heart disease.Methods Coronary angiography and stenting were carried out through femoral artery approach in 331 patients with coronary heart disease.The incidences of in-hospital major adverse cardiac events were recorded all the patients were followed up for 1 year.The follow-up angiography was carried out at at 6-9 months after discharge.Results A total of 597 domestic rapamycine-eluting stents were implanted in 331 patients.One patient died of pump failure after primary PCI and another patient required emergency CABG because of coronary artery rupture.NSTEMI happened in 3 patients after PCI.The incidence of in-hospital MACE was 1.5%.Three hundred and one patients finished a mean follow-up of 12.3?3.2 months.During the follow up period,non-fatal myocardial infrarction was recorded in 1 case,recurrent angina in 16 cases and target lesion revascularization in 5 cases.The overall MACE rate was 4.3%.Follow-up angiography was completed in 110 patients and the restenosis rate was 5.5%(6/110).Conclusion Domestic rapamycin-eluting stent(Firebird stent) was safe and effective in the treatment of coronary heart disease and may provide a higher cost-effectiveness due to its relatively low price.However,its long-term effect needs more studies to verify.
9. Structure identification of primary degradation impurities in domestic calcitonin salmon injections by LC-MS
Chinese Pharmaceutical Journal 2015;50(2):174-177
OBJECTIVE: To establish an LC-MS method for identification of the primary degradation impurities in domestic calcitonin salmon injections. METHODS: For the RPLC analysis., the separation was performed on Waters XTerra RP C18 column (3.0 mm × 50 mm, 3.5 μm) with linear gradient elution using 0.05% aqueous trifluoroacetic acid (A) and acetonitrile (B); the flow rate was 0.5 mL · min-1; the column temperature was 40℃; the detection wavelength was set at 220 nm. For the SEC analysis, the separation was performed on TSK GEL 2000SWxl column (7.8 mm × 300 mm, 5 μm) with mobile phase consisting of TFA-water-acetonitrile(0.05:80:20); the flow rate was 0.7 mL · min-1; the column temperature was room temperature; the detection wavelength was set at 220 nm; the main impurities were collected and identified by positive mode. RESULTS: The structures of three impurities were elucidated by RPLC-MS and SEC-MS. CONCLUSION: The results of this study can be applied in impurity profile analysis, specification research, and quality control of calcitonin salmon injections.
10.Research on the relationship between ecchymosis physique and the uterus myoma
Hui XUE ; Xiuying REN ; Lize ZHANG
International Journal of Traditional Chinese Medicine 2013;(6):485-486
Objective To summarize the tending physique of hysteromyoma patients and thus to provide the clinical prevention and treatment methods for the disease.Methods Clinical epidemiology investigation and study methods were adopted to design the unified investigation and study table.Questionnaire survey were performed stochastically to 150 hysteromyoma patients and 150 normal females.Various physique characteristics were recorded.Results Ecchymosis physique were commonly seen in hysteromyoma patients,accounting for 36.7% in all patients.Conclusion The occurrence of hysteromyoma was closely related to ecchymosis physique.