The changes of zine content in serum and feces observed after intragastric administration of a certain dose of dithizone to the pregnant rats in their third trimester for one week, and the developing status of brain of their pups were studied. The result showed that the total fecal exerction of zine during 24 hours increased and serum zine content decreased significantly aftcr continual administration of dithizone for one week in maternal rats. It suggested that dithizone may be used for making animal model of zine deficiency. The body and brain weights, brain protein content and RNA/DNA ratio of the newborn delivered by the zine-deficiency maternal rats decrcased significantly. This indicates that the maternal zine dificiency in third trimester gives side effects on the development of their fctal brain.

Objective To discuss the contrast-enhanced ultrasonography diagnostic value of the liver low-density focus on contrast-enhanced CT. Methods The contrast-enhanced ultrasonography results of 47 cases of the liver low-density focus on contrast-enhanced CT. were retrospectively analyzed. Results The pathological diagnosis in 47 focuses was metastatic carcinoma 20 cases, inflammatory pseudotumour 9 cases,hepatic hemangioma 6 cases, primary liver carcinoma 4 cases, cyst 2 cases, abscess 2 cases, tuberculosis 2 cases, FNH (focal liver nodular hyperplasia) 1 case, local adipose tissue infiltration of liver 1 case. 45 cases of contrast-enhanced ultrasonography diagnosis was consistent with pathological or clinical diagnosis. Cysts and inflammatory pseudotumour had not ultrasound contrast enhancement in the entire phase, but the crossed blood vessels was observed in inflammatory pseudotumour. The local adipose infiltration of liver had synchronous ultrasound contrast enhancement accompanied with liver tissue enhancement. 80% (12/15) of liver metastases of lack of blood supply showed fast contrast enhancement around the tumour edge and homogeneous and light contrast enhancement in the tumour center. 80 % (4/5) of liver metastases of rich of blood supply showed heavy and rapid contrast enhancement in the whole tumour. The enhancement degree in bscess and tuberculosis in the ultrasound contrast enhancement was correlated with necrosis degree, which showed fast and uneven contrast enhancement, hepatic hemangioma showed gradually contrast enhancement from its edge to center, whose enhancement time lasted for longer time than that of metastatic tumour with a statistically difference. FNH showed a typical contrast enhancement pattern, fast enhancement and fast weakening from center to edge. Hepatic cell carcinoma showed fast enhancement and fast weakening in the whole tumour. Conclusion Contrast-enhanced ultrasonography have diagnostic value of CT liver focus of low-density, which is helpful for the differential diagnosis of such focus.

Objective:To observe curative effect of ultrasound guided celiac abscess puncture treatment, and evaluate the clinical curative effect of value.Methods: One hundred and twenty four cases with downward abdominal abscess puncture drainage guided by ultrasound and open drainage were collected randomly and divided into operation group and puncture group to explore the operation method and evaluate the postoperative effect.Results: The No. of puncture group with better curative effect of instituting is 49(79.0%), good effect No. is 11(17.7%), 2 cases (3.3%) got bad effect, fine rate was 96.8%; The No. of operation group with better curative effect of instituting is 10(16.1%), good effect No. is 31(50%), 21 cases(33.9%) got bad effect, fine rate was 66.1%; the results have significant difference(x2=19.270,P<0.01).Conclusion: Ultrasound-guided puncture can avoid blind puncture caused tissue damage, it has small trauma, simple operation, high safety. The characteristics of the curative effect is distinct, and has high value of clinical application.

Objective To investigate the changes of immunologic functions and life qualities after the auto-cytokine induced killer (CIK) cells combined with interleukin-2 (IL-2) being transfused back to the patients with non-small cell lung cancer (NSCLC).Methods Thirty-eight NSCLC patients were enrolled,and 19 patients received not only chemotherapy and radiotherapy but also adoptive immunotherapy with CIK cells combined with IL-2 compared with the other 19 patients who only accepted radiotherapy and chemotherapy.The changes of immunologic functions and life qualities after the CIK cells combined with IL-2 being transfused back to the patients with NSCLC were observed.Immune targets of the treatment group and control group,such as peripheral blood CD3 +,CD4 +,CD8 + T cell percentage,ratio of CD4 +/CD8 + and Th1/Th2,were observed at the end and after 3 month of the radiation and chemotherapy.Results Both of the two groups were neither having obvious adverse reactions.After CIK cells combined with IL-2 treatment,the life qualities of patients improved,such as 18 cases of mental improvement,15 cases of appetite improvement,12 cases of sleep improvement,and 3 cases of fatigue symptoms improvement.The percentage of CD3 +,CD4 + cells in the treatment group increased [(66.39 ± 9.22) ％ vs (42.98 ± 7.23) ％,t =5.45,P =0.00 ; (32.27 ± 3.75) ％ vs (26.38 ±2.51)％,t =5.73,P =0.00],the percentage of CD8 + cells declined [(17.51 ± 1.85)％ vs (20.90 ± 2.31) ％,t =5.21,P =0.00],the rate of CD4 +/CD8 + increased and the rate of Th1/Th2 obversed [(1.86±0.32) vs (1.27±0.19),t=7.13,P=0.00;(1.15±0.48) vs (0.91 ±0.30),t=2.42,P=0.02].There were significant differences between the two groups.Conclusion Adoptive immunotherapy with CIK cells combined with IL-2 is safe in clinical,which can improve the immunologic functions and life qualities of the patients with NSCLC.

OBJECTIVE:To study the release characteristics of Evening primrose oil microspheres in vitro. METHODS:UV spectrophotometry was adopted to determine the accumulated release of microspheres under different media,and the drug release behaviors in vitro were fitted using zero-order,first-order and Higuchi equations. RESULTS:The accumulated release of microspheres in the gastrointestinal liquid (within 6 h) was up to(92.84?0.35)%;under the media of artificial gastric juice,the release of drug conformed to Higuchi equation:Q=5.941t1/2—11.083(r=0.991 7); under the media of artificial intestinal juice,the release of drug was in line with first-rate equation:ln(100—Q)=—0.487 4t+4.588(r=0.996 4). CONCLUSION:This method is simple,accurate and reproducible,and it can be used for the determination of the release rate of the Evening primrose oil microspheres.

Equisetin (EQST) belongs to polyketide (PKS)-nonribosomal peptide synthetase (NRPS) type compound with an inhibitory effect of 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) enzyme activity. This study investigated anti-obesity effect and insulin resistance improvement effect of EQST on high-fat diet (HFD)-induced ob/ob mice model. EQST treatment effectively reduced the body weight gain, fat weight gain and blood lipid content of model mice. All animal experiments were approved by the Medical Ethics Committee of Capital Institute of Pediatrics. EQST alleviated adipose tissue expansion and hepatic ballooning degeneration of model mice, and also effectively controlled the blood glucose content after glucose load and insulin load, showed a significant improvement in obesity and insulin resistance. EQST inhibited adipogenic proteins fatty acid-binding protein 4 (FABP4) and peroxisome proliferators-activated receptor γ (PPARγ), and upregulated thermogenic protein (uncoupling protein 1, UCP1) through suppressing 11β-HSD1 protein expression. In addition, EQST widely upregulates mitochondrial respiratory metabolism related proteins in adipose tissue and may improve insulin resistance through phosphatidylinositol-3-kinase (PI3K) pathway. Therefore, EQST plays an anti-obesity role by promoting adipose tissue thermogenesis and improving insulin resistance, which may provide reliable clues for improving obesity and diabetes.

Objective To observe the effect of erythropoietin(EPO)on apoptosis and glycogen synthase kinase-3?(GSK-3?)level in newborn rats with hypoxic-ischemic brain damage(HIBD).Methods Thirty 7-day-old neonatal rats were randomly divided into 3 groups:sham-operated group,hypoxic-ischemic(HI)group and EPO group.Rats in HI group and EPO group were subjected to left common carotid artery ligation,and then they were exposed to 80 mL/L oxygen and 920 mL/L nitrogen gas in the sealed containers of 37 ℃ for up to 2.5 hours to establish HIBD models.EPO was injected intraperitoneally into the EPO group rats after operation,Solution was injected into the first 2 groups.All rats were killed at the 24 hours after operations,The apoptosis was identified and analyzed by flow cytometry.The level of GSK-3? was detected by enzyme-linked immumosorbent assay.Results The neuronal apoptosis and GSK-3? content in the cortex and hippocampus tissue in HI group were significantly higher than those in the sham-operated group(Pa

Objective To discuss the uhrasonography and hemodynamic characteristics of carotid atherosclerosis in elderly patients with coronary heart disease (CHD).Methods The 548 people were divided into CHD group (n = 373) and control group (n = 175) by the coronary angiography.And the patients in CHD group were sorted into myocardial infarction (MI) group (n= 160) and non-MI group (n= 213).All patients were above 60 years of age, and underwent carotid artery ultrasound examination.The data of carotid sonography and hemodynamics were classified and analyzed using SPSS 10.0 software.Results The carotid intima-media thickness (IMT), plaque thickness and score were much more in CHD group than in control group [(0.96±0.24) mm vs.(0.78±0.11) mm, (1.90±0.36) mm vs.(1.66±0.13)mm, (2.2±0.7) vs.(1.9±0.4), t= 14.81, 27.89 and 27.83, all P=0.00].A total of 161 (43.2%) patients had atherosclerotie plaques in CHD group, and there were 7 (4.0%) people with plaques in healthy control group(χ~2 =337.18, P =0.00).And there were 81.3% of patients with plaques in MI group, it was more than non-MI group, in which there were 12.9% of patients with plaques (χ~2 =60.88, P=0.00).The carotid peak systolic velocity (PSV) and resistance index (RI) were both higher in CHD group than in control group [(77.0±11.9) cm/s vs.(65.2±3.1) cm/s and 0.77±0.06 vs.0.67±0.08, (t= 12.26 and 50.43, P=0.00)].Conclusions Carotid ultrasonography could evaluate not only CHD, but also CHD severity in the elderly.

[Objective]To evaluate the two plates internal fixation treating the distal humeral comminuted and complicated fracture.[Method]Twenty-eight patient with comminuted distal humeral fracture were treated with the double-plate internal fixation from April 2004 to April 2007.According to AO/ASIF classification method,all fractures were C types.There were 9 C1 types,15 C2 types,4 C3 types.All cases were taken from the middle of posterior elbow incision.The patients were treated with the open reduction and double plate internal fixation.Fifteen cases were done through the triceps tongue-shaped flap,13 cases were olecranon osteotomy (including one case with olecranon fractures with ipsilateral).Surgical steps included:the articular surface reduction of intra-articular fractures,small temporary Kirschner wire fixation,making the complete fractures into simple comminuted fractures of humerl condylar fractures,then resetting metaphysis.Internal fixation method used the restruction plate according to the size of fracture.The restruction plate was fixed in the back of the distal humeral radial column.One-third of a good-shaped tubular plate was used to fix the inner edge of humeral ulnar column,two 90 ? of each other into the plane separately fixed on the inner lateral humeral column.[Result]All cases were followed up for an average of 17 months(ranged,8-33 months).Fracture healing time ranged 8-16 weeks.Myositis ossificans was found in 1 case,joint space narrowing was found in 4 cases.No non-union and ulnar nerve injury were found.The final good-to-excellent rate was 82.1%.[Conclusion]The distal humeral comminuted fracture has complicated structure.The internal fixation with two plates and screws,and early functional exercise could improve the elbow function.It has satisfactory curative effect in clinic.

Objective: An automatic seamless stitching method with spinal X-ray image sequence is presented in this paper. Methods: First, biorthogonal wavelet transform is used to implement decomposing of the multi-resolution and the effective edge of the image can be extracted by this method combined with Canny operator. The feature points of the image can be obtained by calculating the edge contour matrix E and the value matrix H. Second, the roughly matching of feature points can be achieved by using Normalized Cross Correlation (NCC) algorithm and the random sample consensus (RANSAC) algorithm is introduced to remove false matching pairs and to achieve precisely matching. Third, the image sequence is automatically sorted with the improved genetic algorithm to achieve automatic stitching. At last, the weighted average fusion algorithm is appfied to achieve smooth and seamless image stitching. This algorithm is robust for the weak-contrast X-ray image sequence. Results: Experimental results show that high-quality and fast image sequence stitching can be obtained automatically by using this method. Conclusions: To a certain extent, it overcomes the shortcomings of X-ray image sequence such as the strong image noise, concentration of values ofpixels, blurred boundaries, large overlap area and the sequence constraint, and therefore it may be applied to in medical imaging field widely.