To investigate the effects of clonidine on controlled hypotension induced by sodium nitroprusside,twenty-four patients (male 17,femai 7,aged 20 to 58 years,ASA Ⅰ to Ⅱ)scheduled for elective craniotomy,were randomly assigned to two groups:control group (Ⅰ,n=12),clonidine group (Ⅱ,n=12) with premedication of clonidine 5?g/kg P. O.. The MAP of both groups decreased by 40% with the infusion of 0.01% sodium nitroprusside solution (SNP). The results showed that the blood pressure was more easily reduced and maitained in group Ⅱ,and the MAP after discontinuing of SNP in group Ⅱ was lower than in group Ⅰ(P

DICOM is a standard for data format and transmission of digital medical image. DICOM Data Set is a binary data stream using DICOM encoding rule. DICOM Nesting Data Set is a kind of complex Data Set with a tree structure, and is widely used in DICOM services and encoding of DICOM files for its special structure. In this article, the functions and encoding rule of Data Set and Nesting Data Set in DICOM format are presented, and the way of parsing and organizing of them is put forward. The realization method and practical application are also discussed.

Objective To observe the expression and the clinical significance of P 170 and CD 34 in primary acute myeloblastic leukemia (AML)patients.Methods The expression of P 170 and CD 34 was determined by flow cytometry in 30 AML patients.Results The P 170 positive rate was 36.67%,the patients with the P 170 positive expression had higher resistant rate to chemotherapy as compared with P 170 negative group(P

Objective To compare the gas and heat exchange performance of membrane oxygenator DDMO-100S and membrane oxygenator Terumo SX-10 in cardiopulmonary bypass(CPB)in children.Methods 39 children aged 3-12 yrs who suffered from congenital heart disease were selected,and the repair of ventricle septal defect or/and atrial septal defect was performed under cardiopulmonary bypass.The 39 children were randomly divided into Group DD(n =21)and Group SX(n = 18).Membrane oxygenator DDMO-100S(Made by Xi'an Daidai,China)was used in Group DD and Membrane oxygenator Terumo SX-10(Made by Terumo,Japan)in Group SX,respectively.The hypothermic cardiopulmonary bypass with high perfusion flow and the cool crystalloid cardioplegia were applied in all children.The artery blood samples were taken for measuring PO2 and PCO2 and the oxygenating index(OI)was calculated by the formulae(OI = PaO2/FiO2).The temperature at nasal pharynx was monitored and the rewarming time was recorded.Results The total priming volume in Group DD was bigger than in Group SX [(742.5 ± 107.3)ml vs(531.3 ± 84.3)ml,P ＜ 0.05].The PO2,OI and PCO2 at 15 min after CPB or at 5 min before cease of CPB had no differences in both group(P ＞0.05).The rewarming time had no difference in both group [(31.5 ± 10.2)min vs(30.61 ± 8.2)min,P ＞0.05].Conclusions Under our observation condition,the gas and heat exchange performance of membrane oxygenator DDMO-100S were the same as membrane oxygenator Terumo SX-10,when it was used in cardiopulmonary bypass in children,but it had a little bit more priming volume.

Objective:To prepare PLGA nanoparticles modified with folic acid conjugated chitosan oligosaccharide containing pa-clitaxel (F-CS-PLGA-NPs) and study the inhibitory effect on SKOV-3. Methods:F-CS-PLGA-NPs were prepared by an interface dep-osition method, 30% ethanol was used as the release medium for the in vitro release profiles of nanoparticles, and MTT was adopted to evaluate the inhibitory effect of paclitaxel with different formulations and concentrations on SKOV-3. Results:The particle size and zeta potential of F-CS-PLGA-NPs was (321 ± 0. 76) nm and (22. 6 ± 0. 26) mV, respectively, the drug loading was (5. 1 ± 0. 25)%, and the encapsulation efficiency was (41. 96 ± 1. 96)%. F-CS-PLGA-NPs had a similar in vitro release profiles with the ordinary nanoparti-cles ( PLGA-NPs) . About 35% of paclitaxel was released from the nanoparticles in the initial 24 h, and then a near zero order release at a relative slow rate was shown, and the cumulative release rate in 144 h was about 75%. The results of cell experiments suggested that at the same paclitaxel concentration, the inhibition effect of F-CS-PLGA-NPs group was stronger than that of the PLGA-NPs group and the solution group. The inhibition effect of F-CS-PLGA-NPs could be reduced by free folic acid. Conclusion:PLGA nanoparticles modified with folic acid conjugated chitosan oligosaccharide can increase the targeting efficiency in SKOVS-3 tumor cells.

OBJECTIVE:To optimize the extraction technology of oleanolic acid from Elsholtzia bodinieri V.METHODS:The extraction technology was optimized by orthogonal-test using microwave-assisted dynamic extraction method with oleanolic acid as index;And the content of oleanolic acid was determined by TLC-spectrophotometry.RESULTS:The opti-mum extraction condition was described as follows,the microwave power is600W with methanol as extracting solvent,the flow rate was8ml/min and the inner diameter of extraction tube was10mm.CONCLUSIONS:The microwave-assisted dynamic extraction method is simple,safe,rapid,accurate and with high extraction yield.The optimized extraction technology is stable and feasible,and suitable for industrialized production.

Objective To investigate the correlation of HIF-1α and hypoxia in keloids fibroblasts, and to investigate the mechanism that hypoxia promotes abnormal scarring by HIF-1α pathway. Methods Keloid fibroblasts cultured in vitro were placed in an incubator with different O2 concentrations. After 24 h, the keloid fibroblasts were collected for further study. Western blotting was performed to detect the expression of HIF-1α in the keloid fibroblasts. Results Relative amounts of HIF-1α in keloid fibroblasts cultured under O2 concentrations at 20 %, 10 %, 5 % and 1 % were 0. 007 ±0. 006, 0. 133 ±0. 006, 0. 537±0. 015 and 0. 903±0. 021, respectively. It indicated that hypoxia could increase the expression of HIF-lα in keloid fibroblasts. Conclusions Hypoxia can induce the expression of HIF-1α in fibroblasts of keloids. Moreover, there still is a positive relation between hypoxia and the expression of HIF-1α. Therefore, a close relationship exists between abnormal scarring and HIF-1α pathway by hypoxia.

Objective To evaluate the chnical value of intervention therapy combined with the foot plastic surgery in the treatment of diabetic foot.Methods 67 patients who accepted the intervention therapy combined with the plastic surgery for occlusive lesion of lower limbs arteries were selected.The clinical symptom,ankle brachial pressure index and hemodynamics were observed before and after treatment.All clinical indicators were compared between the therapeutic group and the control group.Results Among 67 patients,63 cases were successful in clinical treatment (the success rate:94.0％).One month,three months,six months after operation,the skin temperature,anklebrachial index,limp distance,clinical symptom,ankle brachial pressure index and hemodynamics were increased.Compared with preoperation,all above-mentioned indicators were improved obviously (P ＜ 0.05,P ＜ 0.01).Conclusion Intervention therapy for diabetic foot has the merits of less trauma,higher effectiveness of treating diabetic foot ulcers,defect repair,maximally reserved limbs and better shapes of limbs,which made both doctors and patients satisfied.It is a new direction for minimally invasive therapy.

BACKGROUND:Zinc can improve bone reaction to titanium implants through regulation of osteoblast activity under the condition of osteoporosis. OBJECTIVE:To study the zinc ions effect on improving the stability of titanium implants under the condition of osteoporosis. METHODS:After removal of the ovary, 28 female Sprague-Dawley rats presented with osteoporosis symptoms for 12 weeks and then, they were randomly divided into two groups. Two kinds of titanium implants with (experimental group) or without zinc (control group) were inserted into the bilateral femoral metaphysis longitudinal y in the ovariectomized rats, separately. Three rats were selected from each group for double fluorescence labeling examination. Mineral apposition rate was calculated at postoperatively 6 weeks. Another four rats were selected from each group for histomorphometry analysis at postoperatively 12 weeks. Biomechanical test was in the remaining rats in the two groups to calculate the maximum push-out force and maximum shear force at 12 weeks postoperatively. RESULTS AND CONCLUSION:The double fluorescence labeling examination revealed that the mineral apposition rate was improved in the experimental group as compared with the control group (P<0.05). New bone formation occurred in the two groups. However, larger new bone amount, higher bone area ratio and bone-to-implant contact were detected in the experimental group (P<0.05 or P<0.01). The maximum push-out force and maximum shear force were also superior in the experimental group to the control group (P<0.05 or P<0.01). These findings indicate that zinc ions are capable of promoting bone formation and improving implant fixation in ovariectomized rats.

Objective To compare the effect of different partition of nucleic acid protein complex on the affinity of enriched library in systematic evolution of ligands by exponential enrichment (SELEX). Methods Two protocols were adopted to select the enriched library to transforming growth factor ? receptor Ⅱ(TGF ? RⅡ). Protocol 1: protein was absorbed on the surface of 96 well plate; then, selection was carried out; the binding nucleotide acids were eluted from the supporter directly. Protocol 2: selection was done in solution; nucleotide acid protein complex was captured in nitrocellulose membrane; the binding nucleotide acids were obtained from membrane. Filter biding assay and gel shift assay were performed to detect the affinity of the enriched ssDNA library from different protocols. Results After 4 rounds of selection, the affinity to TGF ? RⅡ was obviously improved in the enriched library from protocol 2 compared with the initial library, while no such improvement was found in the enriched library from protocol 1. Conclusion In the SELEX experiment, the way of selection in solution, then partition of the binding nucleotide acids in filter is easier to enrich the binding fragment from initial ssDNA random library, compared with the way of fixation of target protein to solid supporter, then selection between the solid phase and liquid phase and elution of the binding nucleotide acids from supporter.