Object detection systems are widely used in many fields. To speed up object detection, a rapid method based on color feature is presented in this paper. Artificial neural network is used for color classification. A series of original objects are gained through searching the most outstanding feature of the marker based on multi-resolution. A set of features obtained from these original objects in the original image, and artificial neural network are used for object classification. Experimental results prove the effectiveness of this method.

Identification and classification of white blood cells are important for clinical diagnosis.Many researchers have been seeking the effective methods for white blood cells' automatic classification based on morphological characters.After cell segmentation,leukocytes' feature acquirement and selection,this paper accomplishes white blood cells' automatic classification using Sugeno-model fuzzy neural network and compares the result with that from classifier of BP network.

DICOM is a standard for data format and transmission of digital medical image. DICOM Data Set is a binary data stream using DICOM encoding rule. DICOM Nesting Data Set is a kind of complex Data Set with a tree structure, and is widely used in DICOM services and encoding of DICOM files for its special structure. In this article, the functions and encoding rule of Data Set and Nesting Data Set in DICOM format are presented, and the way of parsing and organizing of them is put forward. The realization method and practical application are also discussed.

Objective To establish the training objective systems for undergraduates in mili-tary clinical medicine in order to promote the educational reform of military medicine, and to improve the quality of talents training in military medical universities. Methods Literature analysis was made to determine the initial military clinical medical undergraduate training objectives, and a questionnaire was given to experts, on-the-job military doctors, medical graduates to know the real requirements of military doctors' position. A total of 455 valid questionnaires were collected. Then the questionnaire results were discussed and summarized by the method of expert discussion. Finally,. Delphi method was used to determine the ultimate training objective system. Results The ultimate training objective system of military undergraduate clinical medicine contains two aspects, eight fields and sixty-one standards objective systems. Conclusion Organically fusing military medical knowledge, ability and professional quality requirement into two levels and eight modules and clearly understanding the standards and requirements of each module are significantly systematic, advanced and operable, which has important reference value and guiding significance to both the military medical colleges and uni-versities and the local universities.

Many kinds of medical education standards came out at home and abroad in the new era to lead and evaluate the development of medicinal education.The Forth Militay Medical University,after analyzing the characteristics and the insufficiency of these standards,believe that health view,medical model and the other five aspects will be the key factors for the research of military surgeon educational standard for undergraduates majoring in clinical medicine.

We detected the isoniazid resistance in clinical Mycobacterium tuberculosis isolates by high-resolution melting (HRM) curve analysis and assessed the application value of the assay.The isoniazid resistance of 49 M.tuberculosis isolates preserved in laboratory was analyzed by the drug sensitivity test (traditional proportion method).Further analysis was made on the sequencing of the isoniazid resistance determining region in these test strains,and their mutation sites were screened.Specific primers used in the HRM curve analysis were designed based on the screened mutation sites,DNA mutations were assayed in the isoniazid-resistant gene determining region by the HRM curve analysis,and an assessment was made of the detection efficiency of the assay in isoniazid resistance in M.tuberculosis.Results of the drug sensitivity test (proportion method) showed that,of the 49 test strains,there were 20 isoniazid-resistant strains,29 isoniazid-sensitive strains.Results of the sequencing analysis showed that:1) KatG gene had four mutation patterns,i.e.,point mutations at site 234,at sites 234 and 315,at sites 234 and 463,and at sites 234,315 and 463;2) there were three mutations were detected in inhA gene,i.e.,mutations in inhA-8,-15 and-152.Analysis of gene mutation in drug-resistant strains found that of the 20 isoniazid-resistant strains,11 (55 ％) were mutated at codon 315 of KatG gene;6 (30％) were mutated in inhA-15 (4/20),-8 (1/20) and-153 (1/20) of inhA gene;two (10％) were mutated at codon 315 of KatG gene and in inhA-15;in one strain (5％),no mutation was detected in KatG and inhA genes.Through the gene mutation detection,the sensitivity and specificity of isoniazid resistance in M.tuberculosis were 95 ％ and 100 ％,respectively.Results of HRM curve analysis of drug-resistance gene mutations in test strains showed gene mutations were present in 18 strains and absent in 24 ones;referring to DNA sequencing results,the sensitivity and specificity of the assay were 94.7％ and 80％,respectively.Judged by mutations as drug-resistance via the HRM curve analysis,19 resistant and 24 sensitive strains were tested.With the drug sensitivity test results by the proportion method as controls,the sensitivity and specificity of the assay were 95 ％ and 82.76 ％,respectively.Use of the HRM curve in the detection of resistance of M.tuberculosis to isoniazid is characterized by good sensitivity and short time consuming,and has certain value in the rapid diagnosis of isoniazid-resistant tuberculosis.

Objective To investigate the effect of ethinylestradiol and cyproterone acetate tablets combined with metformin on hormone levels of patients with sterility caused by polycystic ovary syndrome.Methods Totally 76 infertile patients with polycystic ovary syndrome were selected and randomly divided into two groups,38 cases in each group.The control group was treated with ovulation promotion therapy,while the observation group was given ethinylestradiol and cyproterone acetate tablets and metformin hydrochloride enteric-coated capsules and ovulation promotion.The levels of sex hormones,insulin resistance index (HOME-IR),fasting blood glucose (FBG),fasting insulin (FINS),ovulation and pregnancy were compared between the two groups before and after treatment,and the incidence of ovarian hyperstimulation syndrome (OHSS) and the incidence rate of adverse reactions were recorded.Results After treatment,hormone indicators in both groups were significantly better than those before treatment,and the indicators in observation group were significantly better than the control group (P ＜ 0.05).After treatment,FPG,FINS and HOMA-IR were significantly lower than those before treatment in both groups,and the indexes in observation group were significantly lower than the control group (P ＜ 0.05).In the observation group,ovulation rate,pregnancy rate and incidence rate of OHSS were significantly better than the control group (P ＜ 0.0 5).There was no significant difference in incidence rate of adverse reactions between two groups (P ＞ 0.05).Conclusion Ethinylestradiol and cyproterone acetate tablets combined with metformin is effective in the treatment of patients with sterility caused by polycystic ovary syndrome,which can effectively improve the level of sex hormone and insulin resistance,and increase the ovulation rate and pregnancy rate.

Objective To investigate the effect of ethinylestradiol and cyproterone acetate tablets combined with metformin on hormone levels of patients with sterility caused by polycystic ovary syndrome.Methods Totally 76 infertile patients with polycystic ovary syndrome were selected and randomly divided into two groups,38 cases in each group.The control group was treated with ovulation promotion therapy,while the observation group was given ethinylestradiol and cyproterone acetate tablets and metformin hydrochloride enteric-coated capsules and ovulation promotion.The levels of sex hormones,insulin resistance index (HOME-IR),fasting blood glucose (FBG),fasting insulin (FINS),ovulation and pregnancy were compared between the two groups before and after treatment,and the incidence of ovarian hyperstimulation syndrome (OHSS) and the incidence rate of adverse reactions were recorded.Results After treatment,hormone indicators in both groups were significantly better than those before treatment,and the indicators in observation group were significantly better than the control group (P ＜ 0.05).After treatment,FPG,FINS and HOMA-IR were significantly lower than those before treatment in both groups,and the indexes in observation group were significantly lower than the control group (P ＜ 0.05).In the observation group,ovulation rate,pregnancy rate and incidence rate of OHSS were significantly better than the control group (P ＜ 0.0 5).There was no significant difference in incidence rate of adverse reactions between two groups (P ＞ 0.05).Conclusion Ethinylestradiol and cyproterone acetate tablets combined with metformin is effective in the treatment of patients with sterility caused by polycystic ovary syndrome,which can effectively improve the level of sex hormone and insulin resistance,and increase the ovulation rate and pregnancy rate.

OBJECTIVE To analyze the varieties,causes and preventions of severe complications in OSAHS patients with delayed extubation after surgery.METHODS The clinical dates of 548 OSAHS patients with delayed extubation after surgery were retrospectively analyzed,in order to explore the incidence,reasons,and the prevention and control of severe complications.RESULTS There were 14 cases with severe complications in 548 OSAHS patients,including 1 case of septicemia,one case of nasal alar scar formation,one case of cerebral infarction,2 cases of massive hemorrhage,2 cases of tube dislocation,2 cases of ventilator resistance,2 cases of apnea,3 cases of ventilator-associated pneumonia.All of the14 cases recovered after dealing with the corresponding measures.No dead happened.CONCLUSION The incidence of complications in OSAHS patients with delayed extubation is relatively high.In order to improve the prognosis,more attentions should be paid to the airway care and manipulation in the course of treatment.When the complications happened,corresponding treatments should be done in time.If the patients' respiratory function and airway patency were normal,extubation as early as possible could reduce the incidence of complications.

Objective:To build a predictive model for symptomatic radiation pneumonitis(RP) using the pretreatment CT radiomics features, clinical and dosimetric data of lung cancer patients by using machine learning method.Methods:A retrospective analysis of 103 lung cancer patients who underwent radiotherapy in the Affiliated Hospital of Jiangnan University from November 2018 to April 2020 was performed. Total normal lung tissues were segmented as an interested volume in pretreatment CT images, and then 250 radiomics features were extracted. The correlations of RP and clinical or dosimetric features were firstly investigated with univariate analysis. Then all clinical data, dosimetric data and CT radiomics features were collected and considered as predictors for modeling of RP grade ≥ 2. Features were selected through LASSO machine learning method, and the predictive model was built. Finally, nomogram for risk of RP were obtained according to the selected features.Results:The result of univariate analysis showed that symptomatic RP was significantly correlated with lung dosimetric parameters including mean lung dose (MLD), V20 Gy and V30 Gy( t=2.20, 2.34 and 2.93, P<0.05). Four features, including lung dose volume percentage V30 Gyand three radiomics features, entropy feature of GLCM, mean and median feature of wavelet histogram were selected among all clinical, dosimetric features and radiomics features. AUC of the predicted model obtained from selected features reached 0.757. For convenient clinical use, the nomogram were obtained, and then personalized RP risk prediction and early intervention could be performed according to this nomogram. Conclusions:Pretreatment CT radiomics and dosimetric features can be used in predicting symptomatic RP, which will be useful for advanced intervention treatment.