Objective :To explore the significance of mitochondrial DNA mutations of articular chondrocytes in the rat model of temporomandibular joint osteoarthrosis (TMJOA). Methods:TMJOA models were created in left sides of TMJ of 15 SD rats by the partial resection of the articular disc.The experimental rats were killed 3 months after operation. After the chondrocytes culture, the entire mtDNA were amplified using 32 pairs of overlapping. DNA fragments showing different banding patterns between normal and experimental mtDNA by temporal temperature gradient gel electrophoresis were sequenced to identify the mutations. Results: Of the 35 heteroplasmic pattern PCR products, 42 novel mutations were found, Majority of the novel mutations were in the tRNA and D-loop regions. Conclusion:The mutations occurred in the mtDNA of TMJOA articular chondrocytes.

Chondromatosis, Synovial ; diagnosis ; therapy ; Humans ; Temporomandibular Joint Disorders ; diagnosis ; therapy

China ; Cone-Beam Computed Tomography ; Face ; diagnostic imaging ; Humans ; Jaw ; diagnostic imaging ; Mouth ; diagnostic imaging

Objective: To study the effect of interleukin 1(IL 1?) on the metabolism of osteoarthritic and normal mature condylar chondrocytes in temporomandibular joints, and investigate the role of IL 1? in the pathogenesis of temporomandibular joint osteoarthritis. Methods: The primary generation of osteoarthritic and normal condylar chondrocytes cultured in the monolayer condition was treated with 20 ?g?L -1 recombined human interleukin 1? (rhIL 1?), and then collected to be detected with RT PCR method for the cellular metabolism including mRNA expression of type Ⅱ collagen, aggrecan, collagenase, insulin like growth factor 1 (IGF 1), and transforming growth factor?1(TGF?1). Results: The normal mature condylar chondrocytes showed the obviously decreased mRNA expression of type Ⅱ collagen and aggrecan after the intervention of exogenous IL 1?, but less influence could be found for the collagenase expression. The osteoarthritic condylar chondrocytes exhibited the decreased mRNA expression of type Ⅱ collagen and collagenase under the effect of IL 1?, while the cellular mRNA expression of aggrecan didn’t change obviously. The intervention of exogenous IL 1? didn't show an obvious influence on the cellular expression of endogenous growth factors such as IGF 1 or TGF ?1 for both the normal and osteoarthritic condylar chondrocytes cultured in vitro. Conclusion: IL 1? could not only disturb the expression of cartilage matrix molecules by the normal condylar chondrocytes, which lead to the lesion of condylar cartilage, but also worsen the abnormal cartilage matrix environment within the osteoarthritic condylar cartilage.

Objective To compare the efficacy of proximal femoral anti-rotation nail and artificial femoral head replacement with bone cement in the treatment of intertrochanteric fracture of femur in elderly patients.Methods70 elderly patients with intertrochanteric fracture of femur were selected as the research subjects,and they were randomly divided into the observation group and the control group according to the digital table,35 cases in each group.The observation group was treated with proximal femoral anti-rotation nail,the control group was treated with artificial femoral head replacement with bone cement.The clinical efficacy of the two groups was observed and compared.Results The operation time,intraoperative bleeding volume of the observation group were (52.63±13.72)min,(115.26±35.17)mL,respectively,which were significantly better than those of the control group[(86.69±21.15)min,(328.72±47.62)mL,t=3.98,9.86,all P<0.05].The time of standing and walking after operation in the observation group were (12.36±3.61)d,(17.51±3.60)d,which were longer than those of the control group[(9.42±3.18)d,(15.18±3.55)d],but the differences were not statistically significant(t=1.36,1.08,all P>0.05).There was no significant difference in the Harris hip score 30 months after operation between the two groups(t=0.18,P>0.05),while the Harris hip score of the observation group after 6 and 12 months were higher than those of the control group,the differences were statistically significant(t=9.04,3.75,all P<0.05).ConclusionCompared with artificial femoral head replacement with bone cement,proximal femoral anti-rotation nail for elderly patients with intertrochanteric fracture of femur has more significant effect,it is worthy of clinical promotion.

Objective To summarize the experience during early stage of postoperative monitoring and treatment for the patients follwing lung transplantation in surgical intensive care unit,and to improve the patient's outcome of lung transplantation.Methods A retrospective analysis of 9 cases of orthotopic lung transplantation admitted to surgical intensive care unit (SICU) of Beijing Anzhen Hospital from May 2005 to May 2011 was made.Results There 5 male patients and 4 female patients with the age 21 -67 (46.1 ± 14.1 ) years old in present group,and among them 6 patients survived (survival rate:66.7％ ).The etiological indication of admission were idiopathic pulmonary fibrosis (n =4),pulmonary lymphangioleiomyomatosis ( n =3 ),and bronehiectasis ( n =2).Bilateral sequential lung transplantation ( n =5 ),single left lung transplantation ( n =1 ) and single rigbt lung transplantation ( n =3) were performed.The postoperative duration of mechanical ventilation,stay duration in SICU and in hospital were 2 -32 d,3 -42 d,and 3 -60 d respeetively.Postoperative complication taking place after surgery were acute rejection in 2 cases and pneumonia 4 cases (3 cases were treated with extracorporeal membrane oxygenator).Postoperative in hospital death was 3 cases of 9 resulting from severe intection (n =2) and cireulation failure ( n =1 ).Conclusion Well-planned postoperative management stroategy in SICU plays an important role in outcome control after lung transplantation,including monitor and respiratory and cardiacvascular management,reasonable implementation of vasoactive medications and diuretics,protective mechanical ventilation strategy,reducing the time of ventilation.

0.05).Conclusion:All the results of the five measurement methods showed that the condyle was located in the center of the fossa with a variation in the healthy adults in intercuspal position.The CBCT image of the sagittal middle layer of the joint could show the joint space accurately and has an important value in the research related to the changes of TMJ space.

Objective: To find out the influence of stabilization occlusal splint on intra-articular pressure of the temporomandibular joint. Methods: A muti-channel measuring and analysis system for intra-articular pressure of the temporomandibular joint was developed in this study. Twenty-two subjects were selected to detect the intra-articular pressure of the temporomandibular joint before and after wearing stabilization splint. Results: Before splint wearing, the intra-articular pressure of the temporomandibular joint at intercuspal occlusion status was 61.3?48.5 mm Hg (1 mm Hg=0.133 kPa), the negative pressure at open mouth status was 33.2?34.2 mm Hg. After splint wearing, the pressure at intercuspal occlusion status was 39.5?24.5 mm Hg, the negative pressure at intercuspal at open mouth status was 36.3?25.3 mm Hg. The intra-articular pressure of the temporomandibular joint after wearing splint was significantly lower than the pressure before splint was used, the pressure of mouth opening status was stable before and after splint was used. Conclusion: Stabilization occlusal splint can reduce the intra-articular pressure of the temporomandibular joint.

OBJECTIVETo study the angiographic properties of central AVMs in the jaw, and to investigate the efficacy of embolization of them.

METHODSEleven cases of central AVMs underwent angiography and embolization, nine cases experienced surgery after embolization, and the other two cases were embolized alone; all these cases were followed up after treatment. The angiographic features and embolization results were analyzed.

RESULTSLarge venous pouches were angiographically opacified in 9 of 11 AVMs. Of the nine operated cases, curettage was safely performed. The intraoperative bleeding was evidently decreased, and the continuity of the jaw was preserved. Five of these operated cases were free of recurrence during 18 - 27 months in follow-up. The two cases embolized alone were free of recurrence during 24 months' follow-up.

CONCLUSIONSVia arterial and venous approaches, embolization could greatly decrease the intraoperative bleeding and thus help to preserve the continuity and potentiality of development of the jaw for intraosseous AVMs, moreover, it may be curative in some cases.

Adolescent ; Arteriovenous Malformations ; pathology ; therapy ; Embolization, Therapeutic ; methods ; Female ; Follow-Up Studies ; Humans ; Jaw ; blood supply ; Male ; Mandible ; blood supply ; Maxilla ; blood supply ; Treatment Outcome

OBJECTIVETo investigate the dangerous anastomasis between the external carotid artery and the intracranial arteries.

METHODSAngiograms of the external carotid artery in 250 cases were analyzed, including 35 cases of moyamoya and 215 cases of head and neck lesions.

RESULTSThe 35 cases of moyamoya, 14 middle meningeal arteries (MMA) were found to participate in the blood supply of the brain. In addition, 11 superficial temporal arteries and 7 occipital arteries supplied the brain. All the cases with ligated external carotid artery (ECA) had the pharyngo-occipital anastomasis. Moreover, the ophthalmic arteries in three cases were found to originate from the MMA.

CONCLUSIONSThe external carotid artery has a variety of anastomasis with the internal carotid artery (ICA) and the vertebral artery. Under such circumstances, special measures must be taken to circumvent inadvertent intracranial embolization.

Carotid Artery Diseases ; diagnostic imaging ; Carotid Artery, External ; diagnostic imaging ; Cerebral Arteries ; diagnostic imaging ; Humans ; Radiography