Objective To explore the application value of non coherent diffusion weighted imaging(IVIM) model in magnetic resonance body element for differentiating benign and malignant breast cancer.Methods Compar-ison of dynamic enhanced magnetic resonance (DCE -MRI)was conducted in 60 patients with breast occupying lesions,all patients were not treated with biopsy or needle biopsy before MRI scan.The lesions were obtained by oper-ation or biopsy after MRI scan,confirmed by pathology,39 benign lesions(benign group),28 malignant lesions(malig-nant group).IVIM-DWI parameters of two lesions group(D value,D* value and F value)were compared,analyzed IVIMDWI model parameters(D value,D* value and F value)diagnostic efficiency of diagnosis of benign and malig-nant breast lesions.Results Compared with two lesions groups,in the IVIM DWI model parameters,the D value of the malignant group was (0.95 ±0.19)×10 -3 s/mm2 ,which was significantly lower than (1.33 ±0.25)×10 -3 s/mm2 of the benign group (t =3.89,P <0.05),the F value of the malignant group was (8.97 ±2.01)%,which was significantly higher than (7.01 ±2.13)% of the benign group (t =3.93,P <0.05),there was no significant differ-ence in D* values between the two groups (P >0.05).IVIM DWI model in differential diagnosis of breast lesions had high diagnostic efficiency.Conclusion IVIMDWI model in the diagnosis of benign and malignant breast cancer is highly effective,and it has high clinical value.

Antirheumatic Agents ; administration & dosage ; therapeutic use ; Biological Products ; administration & dosage ; therapeutic use ; Drug Therapy, Combination ; Evidence-Based Medicine ; Glucocorticoids ; administration & dosage ; therapeutic use ; Humans ; Immunosuppressive Agents ; administration & dosage ; therapeutic use ; Lupus Erythematosus, Systemic ; drug therapy ; pathology ; Lupus Nephritis ; drug therapy ; pathology ; Severity of Illness Index

Objective To evaluate the value of narrow band imaging(NBI) in the diagnosis of early squamous esophageal cancer and precancerous lesions.Methods Four thousand and fifty-six patients were examined by routine endoscopy,NBI and iodine chromoscopy,one hundred and fourteen lesions in 82 patients were screened.Of all lesions were detected by NBI with magnification and targeted biopsy.The differences among routine endoscopy,NBI and iodine chromoscopy,and the consistency between IPCL and histological findings were assessed.Results Compared to NBI and iodine chromoscopy,especially flat lesions,there was a high missed diagnosis rate in diagnosis of early squamous esophageal cancer and precancerous lesions by routine endoscopy.With NBI and iodine chromoscopy,the incidence rate of lesions was 78.0% and 79.8%,respectively.For iodine staining,85.7% Grade Ⅰ was high grade intraepithelial neoplasia(HGIN),and 66.0% Grade Ⅱ/Ⅲ was low grade intraepithelial neoplasia(LGIN).For NBI,80.4% Grade Ⅰ was HGIN,but there was no specificity in diagnosis LGIN.In appearance of IPCL,92.9% Type Ⅳ/Ⅴ was HGIN,89.4% Type Ⅱ/Ⅲ was LGIN,and it has a relatively better consistency in IPCL with histological findings.Conclusion There is a high detection rate in diagnosis of early squamous esophageal cancer and precancerous lesions by Lugoul's iodine staining and NBI endoscopy.It's probably that IPCL patterns by NBI with magnification can provide scientific basis for both the endoscopic theraphy of early esophageal cancer and the omen of postoperative recurrence.

Background and purpose:The antitumor drugs produce a marked effect through the induction of apoptosis. The resistance to chemotherapy has been found to be associated with abnormal expression of genes related to apoptosis. We designed this experiment to investigate the role of survivin gene in the resistance to chemotherapy. Methods:After being treated with Hydroxycamptothecine (OPT,21.96mmol/L), the apoptosis in gastric cancer cell line BGC-823 cells were evaluated by either flow cytometry(FCM) or the terminal deoxynucleotidy transfer mediated dUTP-biotin nick end labeling(TUNEL). The expression of Survivin was detected by reverse transcription-Polymerase chain reaction(RT-PCR) and immunohistochemistry.Results:Compared to control group, the OPT can induced apoptosis in BGC-823[(12.68?0.17)% vs (3.35?0.13)% by FCM,(16.00?1.23)% vs (2.78? 0.84)% by TUNEL[, OPT could upregulate survivin expression in BGC-823 cells (1.489?0.041)% vs (0.756?0.037)% in terms of level of mRNA,(0.928?0.046) vs (0.303?0.032) in terms of protein expression).Conclusions:The survivin gene may be associated to the resistance to chemotherapy through the induction of gene expression.

Objective To investigate the effect of artesunate on the expression of heat shock protein 47(Hsp47)in pulmonary fi-brosis in rats.Methods Thirty SD male rats were randomly divided into control group,model group and artesunate intervention group (intervention group).Rats in control group were intrachacheally instilled with saline (0.4 mL),while rats in other two groups 5 mg/kg bleomycin.Rats in both control group and model group were intraperitoneal injected with 1 mL saline per day sub-sequently.And the rats in intervented group were intraperitoneal injected with 10 mg/100 g artesunate per day.The body weight of day 0,day 14 and day 28 were recorded.All the rats were sacrificed on day 28.The pulmonary coefficient and the hydroxyproline content were observed.The expression of Hsp47mRNA and CollagenⅠmRNA were evaluated by RT-PCR.The Hsp47 expression was also detected by Western Blot.The pathological changes were analyzed by hamatoxylin-eosin (HE)stain and Masson stain.Re-sults (1)The body weight of control group grew much faster than other groups (P <0.05).(2)Pulmonary coefficient:model group (12.31±1.89)mg/g>intervention group (8.54±0.67)mg/g>control group(4.81±0.38)mg/g (P <0.05).(3)Ashcroft Scores:model group(5.70±1.09 )>control group (3.08±0.56),model group(5.70± 1.09)>intervention group (4.01 ± 1.25).There was no significant difference between control group and intervention group(P >0.05).(4)Hydroxyproline content:model group (620.33±66.16)μg/g>control group(379.00±35.51)μg/g,model group (620.33±66.16)μg/g >intervention group(429.00± 36.51)μg/g (P <0.05),there was no significant difference between control group and intervention group.(5)Hsp47mRNA expres-sion:model group and intervention group > control group (P <0.05),there was no difference between the first two groups(P >0.05).CollagenⅠmRNA:model group > intervention group>control group (P <0.05).(6)Western blot showed that Hsp47 ex-pression of model group was the highest in all three groups.Conclusion These results indicate that artesunate might inhibit pulmo-nary fibrosis and depress CollagenⅠproduction,and it′s probably a result of Hsp47 down regulation.

The Six Meridians inTreatise on Exogenous Febrile Diseases were once thought the same with the six meridians ofThe Inner Canon of Huangdi. With this theory was overthrown, similarities and differences and the correlation between them have gradually been studied. In this paper, we take the Taiyang Median as an example to explain the similarities and differences from two aspects, including clinical symptoms and the understanding of the same disease with different treatment.

Spindle cell carcinoma of the breast (SpCC) is a very rare variant of breast cancer characterized as large tumor size,high histological grade and high negative expression of hormone receptors and HER2/neu protein.It has a high tendency for local recurrence and distant metastasis,while the axillary lymph node metastasis is less common occured,which leads to poor outcomes.Given the relatively low incidence,our knowledge about SpCC is limited.The main unsolved obstacles are the early dection and diagnosis,and the improvement in treatments.Herein,this article reviewed the clinicopathologic characteristic,diagnosis,treatment and prognosis of the spindle cell carcinoma of breast.

Objective: To explore the clinical effcacy and short-term survival rate of partial cardiac auto-transplantation for treating the patients with giant left atrium.
Methods: A total of 7 patients with rheumatic heart disease received partial cardiac auto-transplantation in our hospital from 2012-08 to 2013-05 were retrospectively studied. The patients were at the age of (54.9±5.0) years including 3 male, all of them combined with giant left atrium and pre-operative atrial ifbrillation (AF), 6 of them received radio-frequency ablation. All patients were monitored for their baseline condition, operative status, peri-operative mortality and complication occurrence for clinical analysis.
Results: All 7 patients received mitral valve replacement, 5 had aortic valve replacement and 2 had aortic valve-plasty, the mean aortic cross clamp time was (271.7±29.0) min. The average left atrial diameter decreased from (91.7±3.5) mm to (64.8±8.1) mm, left ventricular ejection fraction (LVEF) improved from (38.3±6.5) % to (47.4±6.1) % at discharge, allP<0.05; 4 patients recovered to sinus rhythm and no peri-operative death occurred. The patients were followed-up for (20.7±3.9) months, 1 year post-operational survival rate was 100%, 1 patient died at 17 months after operation and the average left atrial diameter was (56.3±3.4) mm in rest 6 patients at the last examination during follow-up period.
Conclusion: Partial cardiac auto-transplantation may obviously reduce left atrial diameter with good clinical effect in certain patients with giant left atrium.

Objective To investigate the correlation between the expression of P-SAPK/JNK and neuronal apoptosis in the striatum during permanent middle cerebral artery occlusion (pMCAO) in rats.Methods Fifty-four male Sprague-Dawley rats were randomly divided into sham operation group and pMCAO 1-,3-,6-,12-,and 24-hour groups (n =9 in each group).Apoptotic neurons in the striatum during cerebral ischemia were detected by TUNEL assay,the nuclear translocation of P-SAPK/JNK in the striatum by immunohistochemical staining expressions of P-SAPK/JNK protein by Western blot.Results The numbers TUNEL- and P-SAPK/JNK-positive cells in the striatum at 1 hour after pMCAO increased significantly (P =0.000 1),and reached the peak at 6 hours.The numbers of TUNEL-positive cells decreased at 12 hours,however,it still higher than the sham operation group (P =0.000 2).Western blot analysis showed that the expression of P-SAPK/JNK after pMCAO increased significantly,and the time-course change was in accord with the result of immunohistochemical staining Neuronal apoptosis in the striatum was significantly positively correlated with the expression of P-SAPK/ JNK (r =0.984,P =0.000 4).Conclusions Cerebral ischemia may induce neuronal apoptosis in the striatum through the activation of P-SAPK/JNK.