Humans ; Neoplasms ; pathology ; therapy ; Pathology, Molecular ; methods ; Precision Medicine

Objective:To observe the effect of He-Ne laser combined with ammonium glycyrrhetate in the treatmnet of 62 patients.　Methods:62 cases of patients with psoriasis vulgar were divided into two groups. 36 cases (A group) received He-Ne laser combined with ammonium glycyrrhetate intravenous infusion, 26 cases(B group) received only ammonium glycyrrhetate. The therapy course of both groups were 30 days.　Results:The total effective rate of two groups was 82.2%. The effective rate of A group and B group were 88.9% and 73.1%, respectively. There was siginfinant difference by the analysis(ridit test)between two groups(U＝2.76，P＜0.01).　Conclusion:The effect of He-Ne laser combined with ammonium glycyrrhetate in the treatment of patients with psoriasis vulglar than that of ammonium glycyrrhetatealone.

Objective To explore the correlation between cytomegalovirus pneumonia (CMV-IP) and viral load in renal transplant recipients and to find out the threshold value of viral load for predicting CMV-IP. Methods The blood samples of 56 renal transplant recipients were taken weekly for the first 2 months and every 2 weeks for 2-6 months after transplantation.Real-time PCR were used to quantify the plasma CMV DNA.The mean viral loads of CMV-IP group and non-CMV-IP group in each time were compared using Wilcoxon test.Different cut-off values were tested to find the suitable values to predict the CMV-IP. Results Of the 56 recipients,8 (14.3%) developed CMV-IP.The viral loads were near zero in the first 4 weeks in both groups;from week 5 the viral load of CMV-IP group increased gradually and reached the climax at week 8 and then declined,but the load of non-CMV-IP group fluctuated at a low level.During weeks 5-11,the viral loads of CMV-IP group were higher than those of non-VMV-IP group.At 5,7 and 9 weeks,the differences of the viral loads between the 2 groups were significant (P

Objective To observe the changes of serum lipids ,hs‐CRP ,MFG‐E8 and Klotho gene levels after rosuvatatin treatment in elderly patients with coronary heart disease complicating hyperlipidemia for investigating the action mechanisms of ro‐suvatatin and its application value .Methods Totally 129 elderly patients with coronary artery disease complicating hyperlipidemia in our hospital were randomly divided into two groups .The control group received only conventional treatment ,while on this basis the rosuvatatin group was given rosuvastatin 10 mg everyday ,with 2 months as a treatment cycle .Blood lipids ,hs‐CRP ,MFG‐E8 and Klotho gene levels before and after treatment were compared between the two groups .The regulation effect of rosuvatatin was investigated Results The blood lipod ,hs‐CRP ,MFG‐E8 and Klotho before treatment had no obvious difference between the two groups .The levels of TC ,LDL‐C and TG after treatment in the rosuvatatin group were significantly decreased compared with the control group ,while the HDL‐C level was significantly increased ,the differences were statistically significant (P<0 .05) .In addi‐tion ,the hs‐CRP level after treatment in the rosuvatatin group was significantly lower than that in the control group (P<0 .05) . Compared with the control group ,the levels of MFG‐E8 and Klotho after treatment in the rosuvatatin group were increased ,the difference was statistically significant (P<0 .05) .Conclusion Rosuvastatin could decrease the blood lipid and hs‐CRP levels ,up‐regulates the MFG‐E8 and Klotho levels ,alleviates the inflammatory reaction and has the anti‐vascular aging effect ,thus effectively treats the patients with coronary heart disease complicating hyperlipidemia .

Objective To establish a UV method of determination of adenosine in Ligia exotica. Methods Adenosine was extracted with 37℃ water and separated by TLC silica gel, the absorbance was detected at 260nm with UV spectrophotometry.Results The cabibration curve for adenosion was linear within the range of 1.0~20.0?g?mL -1, r=0.9998 (n=6), the average recovery was 99.3%,RSD=1.9% (n=3). Conclusion The method was simple, convenient and accurate for determination of adenosine in Ligia exotica.

Objective To study the influence of quedcetin on the collagen synthesis of cultured fibroblasts and to explore the mechanism.Methods The inhibitory effect of quercetin and radiation on fibroblast proliferation was assayed using MTT assay.Collagen synthesis was detected by hydroxyproline colorimetric analysis.Immunocytochemical staining method was used to investigate the expression of collagen Ⅰ and Ⅲ.The mRNA expression of typeⅠ,type Ⅲ collagens and TGF?-1 were assayed by reverse transcriptionpolymerase chain reaction(RT-PCR) and real-time PCR technique.Results Keloid fibroblast cell proliferation and collagen synthesis of fibroblasts were inhibited by quercetin in a dose-dependent manner.Significant inhibition was observed by the treatment with quercetin and radiation together.Immunocytochemical staining indicated the IOD of type I and Ⅲ collagen protein was down-regulated by quercetin and radiation.Both collagen Ⅰ and collagen Ⅲ gene in the quercetin groups showed a significantly decreased mRNA expression compared with that in the untreated group,especially in the group treated with both quercetin and X-ray.Procollagen gene expression was inhibited and then decreased type Ⅰ and Ⅲ protein syntheses of fibroblsts,particularly type Ⅰ procollagen gene(P

Objective To explore the chromosome features of pancreatic cancer cell lines from Chinese patients. Methods G-band Chromosome karyotyping was performed on pancreatic cancer cell lines of PC1, PC2, PC3, PC4 and PC7 that were established in our laboratory. The results of cytogenetic analysis were confirmed by fluorescence in situ hybridization using Chromosome 3, 13 18 and 20 paint probes. Results All the 5 cell lines were hypotriploid and showed a modal number of 58 for PC1, 56 for PC2, 61 for PC3, 53 for PC4 and 54 for PC7 with different proportion of complex chromosome rearrangements including dicentric chromosomes, double-minute chromosomes, ring chromosomes, acentric fragments or complex chromosome translocation, etc. Conclusion Hapotriploid accompanied with complex numerical and structural chromosomal rearrangements is the main cytogenetic marker of chromosomal instability in pancreatic cancer cell lines. Molecular cytogenetic techniques have to be used beside conventional G-band karyotyping for accurate identifying abnormal chromosomes of pancreatic cancer cell lines.

OBJECTIVE To investigate the distribution and the resistance variance tendency of the pathogenic bacteria isolated from the blood cultureing specimens collected during the period of 2002-2005.METHODS A retrospective analysis was made to the blood cultureing results during the period of 2002-2005 with WHONET 5.1 software.RESULTS Gram-negative rods were the predominant bacteria which caused sepsicemia,the isolated rates of Escherichia coli,Klebsiella pneumoniae and Staphylococcus aureus were the most high during the period of 2002-2004 but the S.epidermidis and S.hominis were also the important pathogenic ones which caused blood stream infection.Vancomycin and the teicoplanin were the most effective to the Gram-positive bacteria,and the imipenem and the cefepime were the most effective to the Gram-negative ones.CONCLUSIONS It′s important to strengthen the blood cultureing for blood stream infection patient.

Objective To investigate the role of TRAF6 in NF-?B and AP-1 signaling pathway in human B cell line.Methods Human Ramos B cells were transfected with plasmids expressing YFP fusion dominant-negative TRAF6(DN-TRAF6),or transfected with shRNA-TRAF6 plasmid.After incubation overnight,cells were either sorted with flowcytometry or screened by G418.Activation of NF-?B and AP-1 pathway,including phosphorylation of I?B?,ERK,JNK and P38,as well as nuclear translocation of NF-?B subunits(P65,P50 and c-Rel)and AP-1 subunits(C-FOS,C-JUN,CREB and ATF) were detected by Western blot and ELISA.Results In cells which overexpress DN-TRAF6 or endogenous TRAF6 expression were knocked-down by shRNA,the phosphorylation of I?B?,as well as phosphorylation of JNK were inhibited.Furthermore,nuclear translocation of NF-?B subunits P65,P50,c-Rel,and AP-1 subunits C-FOS and C-JUN were also inhibited in B cells through overexpression of DN-TRAF6.Conclusion TRAF6 selectively activates some kinases in CD40 mediated NF-?B and AP-1 signaling pathway,and plays an important role in their activation.

Objective: To explore influence of coping style on sleep quality and blood pressure in community male population with high normal value blood pressure. Methods: The Pittsburgh sleep quality index (PSQI) and coping style questionnaire (CSQ) were used to assess 120 men with high normal blood pressure in community. With PSQI>7 scores as criterion for judging sleep quality disorders, the subjects were divided into sleep disorder group (n=51) and normal sleep group (n=69), and sleep disorder group received psychological intervention. Results: Sleep disorders existed in 42.5% male population with high normal blood pressure. Compared with normal sleep group, there was significant increase in PSQI [（6.43±2.59）scores vs. (8.33±3.14）scores] and diastolic blood pressure [(81.00±8.91) mmHg vs. (88.00±5.69) mmHg] and significant decrease in factor scores of “problem solving” [(0.76±0.21) scores vs. (0.61±0.18) scores] and “asking for help” [(0.52±0.26) scores vs. (0.41±0.11) scores] in sleep disorder group, P<0.05 all; Compared with before intervention there were significant increase in scores of “problem solving” [(0.61±0.18) scores vs. (0.71±0.12) scores]and “asking for help” [(0.41±0.11) scores vs. ( 0.51±0.13) scores]and significant decrease in PSQI score [(8.33±3.14) scores vs. (7.41±2.37) scores] and diastolic blood pressure [(88±5.69)mmHg vs. (80± 4.17)mmHg] after psychological intervention 12 weeks in sleep disorder group, P<0.05 all. Conclusion: Psychological intervention may improve sleep quality and reduce blood pressure in community male population with high normal value blood pressure.