0.05).Conclusion Omeprazole and Tegaserod improved the reflux symptoms of the NERD patients.Omeprazole improves abnormal acid exposure but not abnormal bile exposure.Tegaserod improved neither abnormal acid exposure nor abnormal bile exposure.

Objective To explore the clinical feature,treatment and prognosis of neonatal small intestinal atresia.Methods Retrospective study was carried out of 80 neonatal small intestinal atresias over the last 14 years,including diagnosis,treatment and prognosis.Results Of them,62 cases were healed(77.5%),18 cases were dead(22.5%),24 cases(30%)associated with other anomalities.The living cases were followed up for 6 months to 3 years.These symptoms had not relapse.Conclusions Satisfactry effect can be obtained by early diagnosis and operative treatment.Sonography is an ideal way for early finding intestinal atresias in a fetus.End-to-end single layer anastomosis is an ideal way for monointestinal atresia,and poly-end-to-end single layer anastomosis plus long gastrointestinal silicone tube for supporting is an ideal way for multipal intestinal atresia.

Objective: To study the treatment of complicated and giant carotid body tumor. Methods: A giant carotid body tumor was dissected 2 years ago. The external carotid artery was embolized by means of catheterization before surgical intervention, and techniques of internal shunting and autogenous blood transfusion was used during the operation. Results: The tumor was resected completely without cerebral vessel disorders and major cephalic nerve injuries. Conclusion: The application of radial intervention of external carotid artery, shunting of internal carotid artery and autogenous blood transfusion are helpful to the treatment of giant and re operative carotid body tumor.

98.95%.The results of certified reference materials were consistent with the target value,and average deviation was -0.31%～1.35%.HPLC was served as the independent variable.When ereatinine was 200 ?mol/L,Bias of Beckman LX20 system,Vitros dry chemistry system and enzymatic method were -10%～13%,-13%～14% and -20%～10%,respectively.Bias of enzymatic method results was mostly negative,when creatinine was

Objective To investigate the roles of interleukin(IL)-25,eosinophils in the occurrence of bronchial asthma.Methods Selected 30 cases of acute episode of asthma(asthma group),and ease a group of 23 cases of asthma treatment(remission group),20 cases of the normal control group.Using the method of gradient ultrasonic atomizing inhalation of Hyperosmotic saline collection of induced sputum specimens,by ELISA method for the determination IL-25 level in induced sputum,count of eosinophils,analysis of correlation between them.Results Group IL-25 in induced sputum of asthma and eosinophil levels[(313.12±75.64)ng/L vs(236.43±57.90)ng/L]were sig-nificandy higher than the control group[(0.386±0.267) × 109/L vs(0.005±0.002) × 109/L],and significantly higher than acute stage in remission stage[(268.63 + 40.19) ng/L,(0.120 + 0.016) × 109/L](P ＜ 0.05).Conclusion IL-25 has an important role in asthma development.It provides a new way of thinking for monitoring and treatment.

OBJECTIVE:To evaluate the curative efficacy of granulocyte macrophage colony stimulating factor (GM-CSF) as an adjunctive therapy for the treatment of acute myeloid leukemia (AML). METHODS: Randomized controlled trials (RCTs) of GM-CSF in the treatment of AML were retrieved from PubMed,EMBase,Cochrane Library,CBMdisc and CNKI database by computer as well as from the leukemia-related journals manually for a quality evaluation of the methodology and Meta analysis using RevMan5.0 software. RESULTS: A total of 10 RCTs were included,of which,3 RCTs were graded as A,5 as B and 2 as C. The results demonstrated that there were no significant differences of indexes among groups as complete remission (CR) rate,relapse rate,infection rate and length of hospital stay,etc. CONCLUSION: The available clinical research results showed that GM-CSF adjunctive therapy could neither increase CR rate,nor decrease the relapse rate,infection rate or length of hospital stay.

Objective To analyze Klebsiella pneumoniae by DiversiLab system, providing scientific evidence for the control of nosocomial infection. Methods Eight strains of non-duplicated clinical Klebsiella pneumoniae isolated from the surgical ward in a hospital in 2010 were typed by rep-PCR-based DiversiLab system. The results were compared with those of pulsed-field gel electrophoresis (PFGE). Results Antimicrobial susceptibility test showed that 7 clinical strains were the same sensitivity to 11 antimicrobial agents, except the strain K8-02. And these 7 clinical strains were all multi-drug re-sistant. The result of PFGE showed that 7 strains were the same pattern. The result of DiversiLab also showed that 7 strains were the same pattern, and the strain K8-02 was another pattern. Conclusion DiversiLab system is simple, quick, good re-peatability and accurate, which is a kind of standardization and automation system. DiversiLab system and PFGE method could get the same result.

Objective To screen the bacteriophage for splitting multiple drug resistance Acinetobacter baumanii to provide a new scheme for the phage therapy of multiple drug resistance Acinetobacter baumanii infection.Methods Multiple drug resistance Acinetobacter baumanii isolated from hospitals in Shanghai served as the host bacteria.The bacteriophages were isolated from different environments.The morphology and size of bacteriophages were observed by electron microscope with negative staining.The influence of pH and temperature on its stability was analyzed.The phage DNA was extracted and sequenced.The related gene function was analyzedResults A virulent phage named D218 of drug-resistance Acinetobacter baumanii was isolated from the effluent sewerage of hospital.The electron microscopy observation showed that it is a long-tail phage of polyhedron symmetric structure,with a diameter of about 65nm,the tail length of about 100nm and width of about 15 nm.The phage kept high activity at pH 3-11 and temperature 4-50 ℃.The genome size is 102449bp with GC content of 37.2%.It encodes 167 ORFs.Conclusion A new virulent phage D218 of drug-resistance Acinetobacter baumanii is isolated,which is stable to pH and temperature,which increases the new data for its application in hospital infection prevention and control,and the phage library setup.

Objective: To express mSDF-1?/GM-CS fusion protein in the Pichia pastoris expression system and investigate its in vivo promoting effects on hematopoiesis function and immune function.Methods: The mSDF-1?/GM-CS fusion gene was chemically synthesized and was cloned into pichia expression vector;the vector was then transfected into Pichia pastoris expression system.Then the expressed products were detected by SDS-PAGE,Western blotting and purified by ion exchange columns.Rat irradiation model was established by 60Co ? ray and were subcutaneously injected with the fusion protein.The hematopoietic and chemotactic activities of the fusion protein were investigated in vivo.Results: The expression vector pPIC9K-SDF1-L-GM-CSF was successfully constructed,and mSDF-1?/GM-CS fusion protein was successfully expressed by Pichia pastoris strain GSl15.The molecular weight of the protein was about 32000 D,with a concentration of 78 ng/ml.The numbers of bone marrow mononuclear cells and GM-CFU were significantly increased in rats after subcutaneous injection of the fusion protein(P

We investigated the serotype,drug resistance features,molecular types,and homology of Salmonella in diarrhea in Tai'an City,and provided scientific evidences for warning,control and clinical treatment.The 31 Salmonella strains isolated from stool specimens of diarrhea in Tai'an were identified by serotyping.Broth dilution technique was used to test the sensitivity of 31 strains of Salmonella to 16 antibiotics.Pulsed field gel-electrophoresis (PFGE) was used for molecular typing and cluster analysis.Results showed that 31 strains of Salmonella were divided into 10 kinds of serotype.S.enteritidis (11/31) was the predominant serotype.All 31 strains of Salmonella were sensitive to cefoxitin and imipenem.The drug resistance rates of the 31 strains of Salmonella were 77.42 ％ (24/31) to sulfafurazole.The 31 strains of Salmonella were divided into 17 patterns by PFGE,with 100％ similarity for each pattern.All the results suggest that there are more serious problems in multiple drug resistance.The genetic variation of S.enteritidis is relatively conservative.Early warning and control on S.enteritidis are exposed to reinforce.