Objective To observe the influence of clopidogrel combined with proton pump inhibitor (PPI) on the postoperative complication of percutaneous coronary intervention.Methods The clinical data of 600 patients who were taking clopidogrel antiplatelet therapy were retrospectively analyzed.All patients were divided into observation group (with PPI,n =300) and control group (without PPI,n=300).In-stent restenosis (ISR) and the adverse event rates were compared between the two groups during the period of follow up.Results The incidence of gastrointestinal bleeding was less in observation group than in control group (6.7％ vs.13.7％,x2 =8.048,P＜0.01),but the incidences of ISR,target vessel revascularization (TVR) and non-fatal myocardial infarction were higher in observation group than in control group(x2 =6.426、5.511、4.718,all P＜0.05).Conclusions Clopidogrel combined with PPI treatment can significantly reduce the risk of gastrointestinal bleeding,but increase the incidences of major adverse cardiovascular events.We suggest that PPI should be used with caution for the patients who need longer-term PPI use.

High myopia is frequently associated with retinal degenerative changes (such as myopic foveoschisis, macular hole and its related retinal detachment, and choroidal neovascularization) which are the major causes of visual loss in high myopic eyes. Optical coherence tomography may help us to understand the macular lesions in high myopia. Peeling of internal limiting membrane and removal of posterior vitreous cortex at an appropriate time, are believed to facilitate restoring the retinal flexibility and resolution of myopic foveoschisis, and promote the macular hole closure and retinal reattachment. Anti-angiogenesis treatment combined with photodynamic therapy and (or) corticosteroid therapy are the future options to treat myopic choroidal neovascularization. Correctly understanding and mastering the methods and timing of diagnosis and treatment of high myopia-related macular degeneration, and taking targeted interventions to enable patients to be rational and effective treated, are the keys to further reduce the damage of visual function in patients with high myopia.

BACKGROUND: As a new-type and effective restoration means of dentition defect and edentulous,implant denture is a restoration method most closely related to natural teeth,and is accepted by more and more patients.OBJECTIVE: To investigate the clinical effects of non-submerged dental implants with sandblasted and acid-etched surface preparation(Straumann implants system).METHODS: Patients who visited Oral Implantology Center of the Affiliated Hospital of Qingdao University Medical College from June 2007 to June 2009 were selected as study subjects.Systemic and local contraindications were excluded.A total of 124patients who had 218 Straumann implants implanted were followed up for 3 to 24 months.According to various patients' conditions,conventional approach,immediately implanted,GBR technique and flapless operation were adopted respectively.Clinical effects were evaluated with the Albrektsson's criteria.RESULTS AND CONCLUSION: There was no implant loosen,shedding or X-ray transmission in 218 implants.Both the survival rate and successful rate of implants were 100%.High degree of satisfaction from patients was achieved.Straumann implants system could be considered as a manageable and satisfactory non-submerged implant system.

Thrombolytic therapy for ischemic stroke has received wide attention. Now the thrombolytic therapy mainly includes intervenous thrombolysis, intra-arterial thrombolysis, combination of intravenous and intra-arterial thrombolysis, and mechanical thrombolysis. The review mainly presents the recent progress in this field.

Objective To study the reverse roles of reverse effect of tetramethylpyrazine on the resistance to adriamycin in human ovarian carcinoma cell line A2780/ADM.Methods The drug sensitivity of A2780/ADM cells was measured by MTT assay.The intracellular concentration of ADM was determined by fluorescent spectrophotometry.The changes of cell apoptosis percentage of A2780/ADM was demonstrated by flow cytometry.Results The IC50 value of TMP in A2780/ADM cell line was significantly decreased by the non-cytotoxic dose or low-cytotoxic dose of TMP.The resistance reversing effect comparing with the IC50 value of ADM cells was respectively 2.07 folds.The intracellular ADM concentration in A2780/ADM cells was significantly elevated by TMP at the same dose and the cell apoptosis percentage was significantly increased by TMP.The expression of P-gp was reduced significantly by TMP.Conclusion TMP could reverse the resistance of human ovarian carcinoma of A2780/ADM cell line to ADM,which may be related to the increased intracellular.

Objective:To determine the expansion and differentiation effect of Staurosporine on human megalaryoblastic cell line(CMK).Methods:With MTT and colony assaies.Results:Staurosporine could densitily inhibited the expansion of CMK cell,IC 50 was 50 nmol/L,Staurosporine could inhibite CMK cell at G 1 phase,then decreased the cells of S and G 2M phases,ST in 80 nmol/L improve the expression of CD41 antigen in CMK cell,induce its differentiation.Conclusion:ST as a new kind of anti-tumor facor has different mechanism with other anti-tumor drugs. [

Objective To investigate the expression of ACTH in normal and hyperplastic adrenal cortical tissue, and to explore the relationship between plasma ACTH concentration and expression level of ACTH in adrenal cortical tissue of patients with adrenal cortical hyperplasia. Methods The expression of ACTH in adrenal cortical tissue was examined by immunohistochemical technique, and the result was analyzed by computer-assisted image analyzing system. Besides, plasma ACTH concentration was measured by RIA. The correlation between plasma ACTH concentration and expression level of ACTH in adrenal cortical tissue of patients with adrenal cortical hyperplasia was studied. Results The expression of ACTH was significantly higher in adrenal cortical tissue of patients with ACTH-independent adrenal cortical hyperplasia than those with ACTH-dependent adrenal cortical hyperplasia and normal adrenocortical tissue (both P0.05). Conclusion ACTH expression exists in normal adrenal cortical tissue. Local ACTH overproduction appears to participate in the pathogenesis of ACTH-independent adrenal cortical hyperplasia.The raised concentration of plasma ACTH influences the expression of ACTH in cortical tissue of patients with ACTH-dependent adrenal hyperplasia.

Objective:To detect the expression of cyclin D1 protein and Rb protein in breast cancer and analyze its relationship to carcinogenesis and development.Methods:The expression of cyclin D1 and Rb protein were detected in 52 breast cancer and 20 benige breast tissues by S-P immunohistochemical method.Results:The positivity rate of cyclin D1 overexpression in the breast cancer was 34.6%(18/52),while there was a significant difference compared with that of benign breast tissues 10%(2/20),P

Objective:To observe the effect of eupolyphaga seu steleophaga(ESS) on the proliferation of autosom al dom inant polycystic kidney disease(ADPKD) cyst- lining epithelial cells.Methods:ESS serum and control serum were obtained from SD rats gastrogavaged with ESS water solution and physiological solution.ADPKD cyst- lining epithelial cells were treated with RPMI16 4 0 containing:(1) EGF0 ,2 .5 ,5 ,10 ,2 0 ng/ ml;(2 ) ESS serum and control serum(1% ,2 .5 % , 5 % ) ;(3) EGF10 ng/ ml,ESS serum and control serum(1% ,2 .5 % ,5 % ) .The cell proliferation was determined by Brdu incorporation assay. Results:EGF had a stimulating effect on the ADPKD cyst- lining epithelial cells;the ESS serum significantly inhibited the proliferation of ADPKD cyst- lining epithelial cells compared with control serum;ESS serum also significantly inhibited the proliferation of ADPKD cyst- lining epithelial cells activated by EGF(10 ng/ m l) com pared with control serum.All the effects were dose- dependent.Conclusion:Eupolyphaga seu steleophaga may prolong the progression of ADPKD via the inhibitory effect on the proliferation of cyst- lining epithelial cells. [

Aim To observe the effect of DL-buthionine s ulfoximine(DL-BSO) on the radiosensitivity of rat C 6 glioma cells under the a erobic and the hypoxic condition. Methods The source of radiati on was 60Co ?-rays. The rats C 6 glioma cells were treated by radia tion alone or DL-BSO+radiation under the aerobic and the hypoxic condition. Col ony forming assay was used to measure effects of DL-BSO on the radiosensitivity . Results Radiosensitive effect of DL-BSO was time-depedent u nder the aerobic condition. After treatment with 0.1 mmol?L -1 DL-BSO fo r 2, 6, 12 hours, the radiosensitive effect was not observed, whereas an enhance ment of radiosensitivity was seen at 24 and 48 hours. An enhancement of radiosen sitivity was seen at 2～48 hours after treatment with 0.1 mmol?L -1 DL-B SO under the hypoxic condition. The radiosensitive effects related to DL-BSO co ncentration under the aerobic and the hypoxic condition. Conclusion Both under the aerobic and the hypoxic conditions DL-BSO can increase the radio sensitivity of rat C 6 glioma cells. DL-BSO increased the rat C 6 gliom a cells radiosensitivity especially under the hypoxic condition, and radiosensit ive effect of DL-BSO is time and concentration-dependent.