Objective: To observe the effect of GETO on learning and memory of experiment AD model rats and search its cholinergic mechanism of action.Methods: ?-amyloid protein(A?_(1-42)) injection into hippocampus in rat was applied to make experimental rat model of AD.Morris water maze test were employed to investigate the function of learning and memory in AD model rats after 4 weeks,and immunohistochemical stain was done with Choline Acetyltransferase(ChAT) and nerve growth factor(NGF) antibodies after Morris water maze test,and system of image pro-plus were used to gain the number and grey degree of positive neurons.Results: In Morris water maze test: The mean escape latent period of the Model rats obviously prolonged than that of the Normal rats,the number of traversing flat roof obviously decreased.The mean escape latent period and the number of traversing flat roof of the GETO rats significantly shorten than the model rats.Immunohistochemical test: The number of positive staining neuron in hippocampus CA1 of ChAT and NGF in the GETO rats was not significantly different from those in the Normal rats and the Donepezil rats,but significantly different from those in Model rats with injection of A?_(1-42)(P

AIM: To determine the contents of baicalin, berberine, rhein in Yiqing Capsule(Radix et Rhizoma Rhei, Radix Scutellariae, Rhizoma Coptidis) by HPLC. METHODS: The HPLC system consisted of Kromasil C 18 column (150 mm?4.6 mm, 5 ?m), 0.02 mol?L -1 phosphoric acid (TEA adjusted to pH=6.82)-acetonitrile(40∶11) mixture as mobile phase, detection wavelength at 254 nm, flow rate of 1.0 mL?min -1. RESULTS: The calibration curves of three ingredients were linear within the range of 3.666-73.32 mg?L -1(r=0.9998, baicalin), 0.3112-15.56 mg?L -1(r=0.9998, rhein), 1.221-50.04 mg?L -1(r=0.9999,berberine), respectively. The average recovery of baicalin is 102.3%(RSD=2.5%), berberine for 101.8%(RSD=1.8%), and rhein for 98.3%(RSD=2.9%). CONCLUSIONS: The method is rapid, sensitive and accurate.

The article describes the development of new magnetic bead separation and purification instrument. The main application of the instrument is to capture tubercle bacillus from sputum. It is a pretreatment instrument and provides a new platform to help doctors to diagnose bacillary phthisis. Not only could it be used for tubercle bacillus capturing, but also for gene, protein and cell separating and purification. Because the controller of the instrument is 16-bit single chip microcomputer, the cost could be greatly reduced and it will be widely used in China.
Cell Separation ; instrumentation ; Equipment Design ; Magnetics

Objective To explore the diagnostic value of indirect magnetic resonance(MR) arthrography in detecting meniscus tears of the knees.Methods Routine plain MR of the knees followed by indirect MR arthrography was performed in 56 knees with suspected meniscal tears clinically.All cases had subsequently arthroscopic examination or operation.The sensitivity and diagnostic accordance of routine plain MR and indirect MR arthrography were compared based on the result of arthroscopic examination or operation. Results The signal intensity in the area of meniscal tears on image of indirect MR arthrography was obviously higher than that of routine plain MR.The diagnostic accordance rate of indirect MR arthrography was 78.67% while the routine plain MR was 52.20%,and the difference was statistically significant(?~2=21.066,P

Tsutsugamushi disease is an acute infectious disease caused by Rickettsia tsutsugamushi.The clinical symptoms of tsutsugamushi disease are varied and non-specific,such as sudden onset fever with chills,rash,skin eschar of ulcer,lymphadenectasis,et al.Patients could combine with multi-organ dysfunction,include respiratory failure,heart failure,mild renal or hepatic dysfunction,circulatory shock or hematological abnormalities.Since the symptoms and signs are non-specific and resemble other tropical infections like malaria,enteric fever,dengue or leptospirosis,appropriate laboratory tests are necessary to confirm diagnosis,and reduce misdiagnosis and missed diagnosis rate.The mainstay of treatment is the tetracycline,chloramphenicol,macrolide and quinolone group of antibiotics.In mild cases,recovery is complete.In severe cases with multi-organ failure,mortality may be as high as 24％.Improve the early diagnosis and timely treatment of the disease,for improving the prognosis of patients,reduce the disease burden is of great significance.

Chronic constipation is a common clinical disorder,which has different influences on patients’quality of life and work. With the increase in pressure and pace of modern life,the aging of population,the changes in dietary pattern and the challenge of social psychological problems,the overall prevalence of chronic constipation in general population is increasing in recent decades. However,the therapeutic efficacy of chronic constipation is still not satisfactory. This article reviewed the advances in study on treatment of chronic constipation.

AIM: To observe the effects of Yangxue qingnao-containing serum on rat vascular smooth muscle cell (VSMC) proliferation induced by lysophosphatidic acid (LPA). METHODS: The [ 3H]-TdR incorporation and mitogen-activated protein kinasc (MAPK) activity were measured in cultured VSMC. End product of lipid peroxidation-MDA levels were also detected. RESULTS: 1?10 -9 ,1?10 -8 and 1?10 -7 mol/L LPA enhanced the cultured VSMC [ 3H]-TdR incorporation, increased MAPK activity and MDA content in a concentration-dependent manner. 5%, 10% and 15% Yangxue qingnao-containing serum concentration-dependently inhibited the increase in VSMC [ 3H]-TdR incorporation, MAPK activity and MDA content induced by LPA. CONCLUSIONS: LPA has a stimulating effect on VSMC proliferation. The LPA-induced intracellular signal transduction may be related to MAPK activity. Yangxue qingnao can efficiently inhibit LPA -induced VSMC proliferation,MAPK activity and lipid peroxidation. [

Objective To investigate the effect of Brocho-Vaxom in curing chronic pulmonary heart disease and the influence of patient's immunity.Methods 58 patients with chronic pulmonary heart disease were randomly divided into two equal groups.Each patient of treatment group took a Brocho-Vaxom capsule(7mg/cap) every.The respiratory tract infection times and duration were observed.The serum IgA,IgG,IgM level were tested by fast immunonephelometry.The sub-type lymphocyte was tested by SP,and the NK cell's activity was tested by MTT.Results The infection time obviously declined in treatment group which the average was (2.5?1.8) in five months as compared with that in the controlled group(P

Bone marrow mesenchymal stem cells (BMSCs) are a group of pluripotential non-hematopoietic somatic stem cells niched in bone marrow.With the characteristics of stable genetic traits,pluripotential in differentiation,easy to isolate from source tissue,and fast to proliferate when cultured in vitro,BMSCs are currently attracting extensive research interests,and considered to be one of the most promising candidates in corneal tissue engineering.At present,many research groups,domestic and abroad,have reported that BMSCs can not only differentiate into corneal limbal stem cells,corneal epithelial cells,and corneal endothelial cells,but also play an important role in ocular surface repair.However,the successful application of BMSCs in cornea usually depends on the correct selection of supporting materials or scaffold,such as xenogeneic corneal stroma and amniotic membrane.Other unsolved problems in BMSCs-related corneal tissue engineering include the molecular biologic mechanism underlying the directional differentiation from BMSCs to corneal cells,the standards to identify BMSCs from differentiated corneal cells,the optimal scaffold materials and the potential tumorigenicity with grafting of transformed or undifferentiated BMSCs.This paper reviewed the progresses and issues of corneal tissue engineering with BMSCs.

Objective To compare Pulmicort and Dexamethasone’s curative effects for acute laryngitis in children. Method Children with acute laryngitis were divided into PUL group and DEX group according their therapy methods. The curative effects and recurrence time were compared. Results The efficiency rate in PUL group was significantly higher than in DEX group (P<0.05). The therapy 3 d and post-therapy dyspnea, hoarseness, bark-like cough and stridor score in PUL group were significantly lower than in DEX group (P<0.05), and the recurrence time of dyspnea, hoarseness, bark-like cough and stridor was significantly shorter (P<0.05). Conclusion Pulmicort could significantly improve the symptoms and signs for children with acute laryngitis with few side effects. Its curative effects are better than dexamethasone.