Protein fingerprinting technology(PFT) is a novel technology for laboratory diagnosis developed in recent five years.It has advantages of simple operation,testing quickly,high sensitivity and specificity.It is a revolutional progress for laboratory diagnosis.The application of PFT in medical field is mainly for the detection of diseases.The sensitivity and specificity in cancer detection are about 80%.Immunomic mass spectrometry(IMS) is a novel technology using the combined group antibodies for capture multi biomarkers and applying mass spectrometry to precisely analyze the modification or isoforms of the biomarker in single platform,whereas traditional assay could not be able to identify the variation of biomarkers.PFT and IMS have significantly influenced in cancer early detection,especially to evaluate cancers which did not express traditional tumor markers like AFP,CEA,etc.PFT and IMS have characteristics of early detection in the molecular and gene level.PFT and IMS are diagnostic technologies with bright future and potential applications.

Objective To investigate the effect of cisplatin on proliferation and invasion of colorectal cancer cell line SW480. Methods SW480 cells were used as the research object, and untreated SW480 cells were used as the control group. Different concentrations of cisplatin was given at different times to intervene. The MTT, Transwell chamber and phos-phate determination methods were used to detect proliferation, invasion and Na+-K+-ATPase activity expression level in SW480 cells. Results The physiological concentration of cisplatin (70 μmol/L) inhibited the proliferation of SW480 cells at 48 h. There was no significant difference in the inhibition rate at 48 h compared with 72 h and 96 h. Treatment with 70 μmol/L cisplatin for 48 h reduced the number of cells through Matrigel membrane matrix. The Na+-K+-ATPase activity was significantly increased in 35, 70 and 140 μmol/L cells after treatment with 17.5, 35, 70 and 140 μmol/L of cisplatin in SW480 cells for 48 h, and Na+-K+-ATPase reached the highest level at 70 μmol/L of cisplatin. Conclusion The decreased activity of Na+-K+-ATPase may lead to the attenuation in proliferation and invasion of colorectal cancer cells, which may be associated with cisplatin resistance in colorectal cancer cell SW480.

The clinical supervision of counseling and psychotherapy have a closely connection with clinical employment.Leading the way in clinical supervision,many clinical supervision models has been extrapolated by theories of various psychotherapy,relating to psychoanalysis theory,person-centered approach theory,cognitive and behavior psychotherapy theory,system theory,structuralism theory,and so on.Recently,especial,different clinical supervision models have to expand beyond psychotherapy,such as developmental model of supervision and society role model of supervision.The study has summarized clinical supervision models of the field,in order to offer literature index and concept frame.

Animals ; Dental Implantation, Endosseous ; Dental Implants ; Dental Implants, Single-Tooth ; Denture, Complete, Immediate ; Denture, Partial, Immediate ; Humans ; Immediate Dental Implant Loading ; methods ; Jaw, Edentulous ; rehabilitation ; Jaw, Edentulous, Partially ; rehabilitation ; Mandible ; Maxilla

Objective:To probe into the influences of psychosocial risk factors and family psyche environment on smoking behavior of adolescents.Methods:Psychosocial risk factors and family psyche environment of 806 adolescents with smoking behavior and that of 3603 controls were investigated with Family Environment Scale, Symptom Checklist 90 and Behavior Questionnaire.Results:Rebelliousness, Risk Taking, Early Maturity, Problem Helplessness of adolescents were related to smoking. The scores of cohesion, expressiveness, achievement orientation and intellectual-culture orientation in Family Environment Scale of the smoking group were lower than those of controls (P

Objective To analyze the roentgenographic findings of the osteopetrosis. Methods Nine patients with clinically suspected osteopetrosis underwent the X-ray examinations of the chest,skull,pelvis,vertebral column,femur,tibiofibula,bones of upper limb and bilateral handbone. The roentgenographs of all patients were analyzed together with review of the literature. Results The basic image feature of osteopetrosis is bone density increased notably,cortible bone thickening,cavum ossis narrowing or occluded and mark X-ray signs include sandwich change in back bone,concentric rings change in pelvis,bone within a bone change,bright-dark alternation transverse striation in metaphysis of tubiform bone,baseball club appearance due to modelling deformity of long bones and periosteal new bone formation. Conclusion Osteopetrosis is a rare inherited disorder. Its mark roentgonographic findings are important evidence for performing the definite diagnosis and differential diagnosis.

As no fiber sheath covered,mesopancreas is considered to be different from mesocolon and mesorectum.Though amount of lymphatic,nervous,vascular and fat tissues are consisted in this structure,the margin and range are still controversial in the clinical practice.Only a few clinical reports show a higher R0 resection rate with the procedure of total mesopancreas excision (TMpE),but most of the studies are singlecentered and retrospective,from which we could not assess the outcome improvement for the patients undergoing TMpE.Moreover,the difference between the ranges of TMpE and ever-used extended excision or lymphectomy should be further evaluated.More anatomical and clinical studies are needed to standardize the operation range in order to benefit the patients.

Objective]To explore and analyze its original implication of the eighth article in Chapter 22 from Jinkui Yaolve. [Methods]Through learning the Jinkui Yaolve closely and referring to the explanations of masters of generations,base on the original text to analyze and elaborate the etiology, pathogenesis, syndromes and therapeutic principles with regard to miscellaneous gynecological diseases.[Results] The eighth item is the general program of miscellaneous gynecological diseases,its principal pathogenic factors include deficiency, stale chills and stagnation of Qi.Moreover,the deficiency plays a leading role in the pathogenesis.Through exploring deeply,I hold that the pulmonary abscess is actually the pulmonary asthenia in the second sentence.When the pathogenic factors attack the body,the triple energizers manifest different symptoms and signs.There is no difference between male and female on the conditions which is confined to the upper warmer and the middle warmer.Such symptoms as pulmonary asthenia,chill colic,hypochondriac pain and abdominal pain,aching at the Guanyuan aera below the navel, asthenic fever,scaly dry skin etc.Their special dissertation distributes in other chapters of Jinkui Yaolve.Yet,the symptoms in the lower warmer which are only seen in the female,owing to deficiency-cold,blood-stasis and stagnation.[Conclusion] In history, Jinkui Yaolve firstly puts forward pulse syndrome complex and treatment of miscellaneous gynecological diseases,the core ideas on miscellaneous gynecological diseases of Zhang Zhongjing also have reference significance for the present.

Oxidative stress phys a very important role in the cerebral ischemia-reperfusion injury.The role of NADPH oxidases (NOX) in oxidative stress after ischemic stroke has been paid more attention and may become a new therapeutic target.This article sums up the major research progress between NOX and cerebral ischemia-reperfusion injury,discusses the characteristics of various isozymes and their relationship with stroke,further investigates the pathogenesis of stroke from the perspective of NOX,and provides a new idea for the prevention and treatment of ischemic stroke.