Human metapneumovirus (HMPV)is a recently discovered virus associated with respiratory tract infection. Most infections with HMPV occurred during winter and spring. Children, elderly subjects and immunosuppressed hostsare susceptible. The consequences of HMPV infections ranged from mild upper respiratory tract infection to severe bronchiolitis or pneumonia. There are three different kinds of methods for the diagnosis of HMPV infections, detection of virus antigen-antibody, virus isolation by culture, and RNA detection by reverse transcription-PCR. Symptomatic therapy is the main method of treatment.

Objective To evaluate the current situation of autoantibodies detection in China in order to improve the quality of detection. Method Inviting letters to partitcipate this evaluation were sent to all laboratories that detect auto-antibodies. Those responding laboratories were enrolled to this study. The testing items included antinuclear antibody, anti-dsDNA antibody, anti-ENA antibody, anti-mitochondrial antobody and anti-smooth muscle antibody. The distribution of samples and the analysis of results were both double-blinded. Result The correct rate of ANA, anti-dsDNA antibody, anti-ENA antibody, anti-mitochondria antibody and anti-smooth muscle antibody was 85.4%,81.8%,36.9%, 66.7%, and 50% respectively. Conclusion Comparing with the first quality control results, the overall results is this evaluation have been improved, but there is still a longway to go.

Objective To investigate the relationship between amniotic fluid glucose concentration, amniotic fluid volume and neonatal birth weight in gestational diabetes mellitus (GDM). Methods Two hundred and fifty-five singleton, normal term pregnant women were divided into three groups: GDM, gestational impaired glucose tolerance (GIGT) and normal pregnancy according to the results of a 50 g, 1 hour glucose challenge test (GCT) or of a 75 g oral glucose tolerance test (OGTT). There were 85 study subjects in each group. All women had GCT at 24-28 gestational weeks. When they had a positive GCT, in which glucose level was ≥7.8 mmol/L,and 0.05), but was significantly higher than that in normal group, (12.7?3.2)cm (P0.05). (4) Among women with GDM, AFG was significantly correlated with AFI (r=0.330, P=0.002), NBW (r=0.347, P=0.001), MFG (r=0.589, P

Objective:To evaluate the clinical efficacy of dezocine, fentanyl and sufentanil at the equivalent dose on hemodynam-ics, sedation and analgesia in fibrobronchoscope-guided. Methods:A total of ninety patients with anticipated difficult airways expected to be nasally intubated using fibrobronchoscope were enrolled and randomly divided into three groups:dezocine group ( group D) , fent-anyl group ( group F) and fentanyl group ( group S) . Ten minutes before the intubation, each group was intravenously given dezocine 0. 15mg ·kg-1 , fentanyl 3μg ·kg-1 and sufentanil 0. 2μg·kg-1 , respectively. The values of HR, MAP and SPO2 were recorded respectively at the time when the patients entered the operation room ( T0 ) , before the bronchoscopy-guided tracheal intubation ( T1 ) , after the intubation ( T2 ) and 5 minutes after the intubation ( T3 ) . The Ramsay sedation score before the intubation and adverse reac-tions during the intubation were also observed. Results: In group F and group S, HR and MAP decreased significantly at T1 ( P<0. 05). The levels of SPO2 decreased significantly at T1 and MAP increased significantly at T2 in the three groups (P<0. 05). The levels of HR, MAP and SpO2 in group F and group S were lower than those in group D (P<0. 05). The levels of HR and MAP in group F at T2 were higher than those in group D and group S, and SPO2 was dropped significantly in group F and group S (P<0. 05). The levels of HR in group S dropped significantly, and were lower than those in the other two groups. MAP in group F was higher than those in the other two groups (P<0. 05). From the Ramsay sedation analysis, the scores of the three groups increased significantly and the score in group S was higher than that in group F (P <0. 05). The incidence of nausea and respiratory depression in group F was significantly higher than that in group D (P<0. 05). The incidence of agitation in group F was significantly higher than that of group S (P<0. 05). The incidence of respiratory depression in group S was significantly higher than that of group D and group F (P<0. 05). Conclusion:Dezocine combined with surface anesthesia has a slight effect on hemodynamics during the tracheal intubation guided by bronchoscopy in the patients with difficult airways. The incidence of reverse reactions of dezocine is lower than that of fenta-nyl and sufentanil.

Objective To investigate the impact of cerebral microbleeds (CMBs) on cognitive function in ischemic stroke patients.Methods A total of 268 acute ischemic stroke patients recruited in the Ningbo First Hospital from January 2014 to June 2015 were divided into CMBs group (199 patients) and non-CMBs group (69 patients) according to whether complicated with CMBs by susceptibility-weighted imaging (SWI).According to the microbleed number, CMBs group patients were divided into 2 grades: grade 1 (1-5 CMBs) and grade 2 (≥6 CMBs). Montreal Cognitive Assessment (MoCA) was used to evaluate and compare the global cognitive function and cognitive domains of the patients. Results The total MoCA score and the scores of visuospatial/executive, attention domains in CMBs group were 20.95±4.53, 2.53±1.09, 3.83±0.97, while those in non-CMBs group were 26.82±1.25, 3.16±1.24 and 4.91±0.84. The total MoCA score and the scores of visuospatial/executive, attention domains were significantly lower in CMBs group than those in non-CMBs group (t=16.59, P<0.01;t=3.75, P<0.01;t=8.83, P<0.01). The total MoCA score and the score of attention domain in grade 1 CMBs group were 21.53±4.61 and 4.11±0.91 , which were significantly lower than those in non-CMBs group (t=14.09, P<0.01;t=14.23, P<0.01). Whereas the total MoCA score and the scores of visuospatial/executive, attention, orientation domains in grade 1 CMBs group were 21.53±4.61, 2.88±1.06, 4.11±0.91, 4.96±0.40, which were significantly higher than those in grade 2 CMBs group (18.58±3.08, 2.23±0.95, 3.63±1.01, 3.85±0.39, respectively;t=2.85, P<0.01;t=2.54, P<0.05;t=5.63, P<0.01;t=2.58, P<0.01). Multivariate regression analysis showed that independent risk factors of MoCA scores in ischemic stroke patients included CMBs (OR=3.15, 95% CI 1.28-5.12, P=0.005) and the number of CMBs (OR=1.73,95% CI 1.08-2.32,P=0.031). Conclusions CMBs and the number of CMBs were independently associated with cognitive impairment in ischemic stroke patients. And with the increasing of the microbleed number, the impairments of certain cognitive domains were more obviously.

Objective The goal of this work is to evaluate the dosimetric characteristics of a new 2D diode array (Mapcheck from Sun Nuclear Corporation,Melbourne,Florida) and assess the role it can play in routine IMRT QA. Methods Fundamental properties of the Mapcheck such as reproducibility and linearity were studied for high-energy photon beams. Clinical performance of the MapCheck for relative and absolute dosimetry was demonstrated with head and neck IMRT plans,and compared with plan dose distribution. Absolute dose measured by Ion chamber and Mapcheck are compared with the one given in TPS. Results The Mapcheck offers the dosimetric characteristics required for performing both relative and absolute dose measurements. Conclusion Mapcheck can provide the verification of absolute dose and relative dose. Its use in the clinic can simplify and reduce the IMRT QA workload.

ObjectiveTo explore the clinical value of ultrasound in the diagnosis of chest wall tuberculosis.Methods134 cases of chest wall tuberculosis were diagnosed with ultrasound and the correlation with CT,X-ray diagnostic results were studied.ResultsThere were solid echo type 27 cases; liquid dark area type 42 cases and combined echo type 65 cases.There was statistical significant difference between ultrasound and CT diagnosis of calcification within the lesion and the crab-like sinus(P＜0.05).The specificities of ultrasound and CT diagnosis of chest wall tuberculosis were 95.52％ and 93.28％,respectively,and the difference was not statistically significant (P＞0.05).ConclusionUltrasound diagnosis of chest wall tuberculosis has clinical value on determining the size of chest wall nodule,its range,and its relationship with the surrounding tissue.It is an effective diagnostic method of chest wall tuberculosis.

Objective: To manage various medical consumable scientifically in different consumable materials, the new separate-store management of consumable management was developed. Methods: The medical consumable depot could be divided into the general consumable depot, the in vitro diagnostic products (IVD) depot and the high-value consumable depot, different management modes could be provided varying from different consumable depots mentioned above. Results:Different items of management processes are regulated, the full-cost accounting of consumables are promoted and the social and economic benefits of hospitals are enhanced. Conclusion: This management mode had the characteristics of normalization and practice.

Objective To describe a modified basin-forming hepatic duct-jejunum Roux-en-Y choledochojejunostomy and explore its clinical application.Methods We retrospectively reviewed clinical data on 57 patients receiving modified basin-forming hepatic duct-jejunum Roux-en-Y choledochojejunostomy in the Department of Hepatobiliary Surgery,People's Hospital of Hunan Province during the period from February 2016 to August 2016.Results Among 57 cases,38 cases previously underwent conventional basinforming hepatic duct-jejunum Roux-en-Y choledochojejunostomy for the reasons such as hepatolithiasis,iatrogenic proximal bile duct injury,congenital choledochal cyst (Todani type Ⅰ),etc.The mean number of operation was 1-4(1.8 ± 1.3).Errors during reoperation can be classified relevant to cholangiojejunostomy anastomosis,bridging jejunal loop and jejunum-bridging jejunal loop anastomosis.Among 57 cases,bile intestinal anastomotic leakage occurred in one patient,incision fat liquefaction in 3 patients,2 patients experienced stress gastritis,all postoperative complications recovered under conservative management.No postoperative mortality happened.During mean (4.07 ± 3.27) months follow-up period,only 4 patients complained of reflux cholangitis,which could be easily managed by antibiotics use.Conclusions By correcting errors in application of conventional Roux-en-Y choledochojejunostomy,a modified basin-forming hepatic duct-jejunum Roux-en-Y choledochojejunostomy proposed in this study demonstrated preliminary better results.

OBJECTIVE:To investigate the effects of minocycline on clinical indexes,inflammatory factors and blood glucose of patients with moderate and severe chronic periodontitis complicated with type 2 diabetes(T2DM). METHODS:A total of 125 patients with moderate and severe chronic periodontitis complicated with T2DM selected from our hospital during Feb. 2013-Aug. 2015 were divided into control group(63 cases)and observation group(62 cases)according to random number table. Both groups were periodental non-surgical treatment as scaling and root planning. Control group was given iodine glycerin to fill periodontal pockets after surgery,2-4 times/d. Observation group was given Minocycline hydrochloride ointment,once a week,after surgery. Both groups were treated for 4 weeks. The levels of clinical indexes [probing depth,clinical attachment level(CAL),probing bleeding rate],serum inflammatory factors(CRP,TNF-α)and blood glucose(fasting blood glucose,glycosylated hemoglobin) were observed in 2 groups before treatment,3 months after treatment,and the occurrence of ADR was recorded. RESULTS:Be-fore treatment,there was no statistical significance in clinical indexes,serum inflammatory factors and blood glucose levels be-tween 2 groups(P>0.05). Three months after treatment,probing depth,probing bleeding rate and serum inflammatory factor lev-els of 2 groups were decreased significantly,and the observation group was significantly lower than the control group,with statisti-cal significance(P<0.05). Compared to before treatment,CAL of 2 groups were increased,without statistical significance before and after treatment or between 2 groups(P>0.05). The levels of fasting blood glucose in 2 groups were decreased,with statistical significance(P<0.05),but no statistical significance between 2 groups(P>0.05). Compared to before treatment,the level of glycosylated hemoglobin in control group was decreased,without statistical significance(P>0.05);the level of glycosylated hemo-globin in observation group was decreased significantly,and the observation group was significantly lower than the control group, with statistical significance(P<0.05). There was no statistical significance in the incidence of ADR between observation group (3.23%) and control group (0)(P>0.05). CONCLUSIONS:For moderate and severe chronic periodontitis complicated with T2DM,minocycline effectively improves clinical indexes of periodontitis,reduces serum inflammatory factors and blood glucose with good safety.