Objective To improve the quality and santdard of medical French bilingual teaching and promote the learning ability of the students in medical universities. Medthods Analogy between English and French was applied to the intervention of bilingual teaching in the clinical students who were learning medical French. The advantages and disadvantages were analyzed.Results The students could apply their prior English to medical French learning and make progress in both languages. A series problems were found during the practice:arrangement of teaching contents, lack of teaching materials and shortage of teaching staff. Conclusion Analogy between English and French is an effective teaching method in both foreign language teaching and bilingual teaching. But the expected teaching goals can be achieved only by formulating reasonable teaching contents and strategies and mproving teachers’ quality.

Objective:To investigate whether celecoxib(CXB), a specific COX-2 inhibitor,can inhibit the proliferation of cyst lining epithelial cells through blocking mitogen-activated protein kinase(MAPK) signal transduction pathway.Methods: Primarily cultured cells were treated with different concentrations of CXB(0,2.5?10~(-6),5?10~(-6),1?10~(-5),2?10~(-5),3?10~(-5),4?10~(-5),5?10~(-5)mol/L) and the proliferative status was evaluated by BrdU assay.The levels of vascular endothelial growth factor(VEGF) were measured by enzyme-linked immunosorbent assay(ELISA);the production of proliferating cell nuclear antigen(PCNA) and phosphoMAPK were measured by real-time reverse transcription-PCR assay;and the expression of PCNA,MAPK and phospho-MAPK protein was detected by Western blotting.Results: BrdU assay revealed that CXB inhibited cell growth in a concentration-dependent manner;the maximum inhibition rate([63.9?1.2]%) was found when cells were treated with 2?10~(-5) mol/L CXB for 24 h.VEGF secretion by cyst lining epithelial cell was reduced by CXB in a concentration and time-dependent manner.The mRNA and protein levels of PCNA,phospho-MAPK in CXBtreated group were lower than those in control group(with no CXB treatment).Conclusion: CXB can obviously inhibit the proliferation of cyst lining epithelial cell and the secretion of VEGF,which might be through interfering with the phosphorylation of MAPK and partly blocking MAPK signal transduction pathway.

Objective: To investigate the effect of celecoxib (CXB), a specific COX-2 inhibitor, on the proliferation of Han: SPRD-cy rats' renal cyst, and to probe into the related mechanism. Methods: Totally 57 3-week-old male Han: SPRD heterozygous(cy/+) rats were randomly divided into 3 groups (n=19): control group (fed with normal forage), low dosage CXB group (3 mg·kg -1·d-1) and high dosage CXB (10 mg·kg -1·d-1) treatment groups. The animals and their bilateral kidneys were weighed; the 2-kidney weight/total body weight (2K/TBW) ratio was calculated. The renal cystic index (CI), fibrosis index, and inflammatory cell infiltration in interstitium were observed by pathologic renal tissue slices. The co-expression of PCNA and COX-2 was analyzed by double immunofluorescence labeling technique and laser scanning confocal microscopy. The expression of PCNA and COX-2 protein was examined by Western blotting analysis. Results: The 2K/TBW ratio of the low dosage group ([1.10±0.009] %) was significantly lower than that of the control group ([1.33±0.02]%) at the 16-week old, (P<0.05). Compared with the control group, the inflammatory cell infiltration in other two groups was significantly decreased ([2.6±0.26], [2.8±0.31] vs [3.7±0.33]), P<0.05). The fluorescence intensities of COX-2, PCNA in the low dosage CXB group was significantly lower than those of the control group (P<0.05). Compared with the control group, the other two groups had significantly decreased expression of COX-2 ([0.326±0.011], [0.409±0.008] vs [0.814±0.012], P<0.05) and PCNA ([0.763±0.051], [0.925±0.042] vs [0.988±0.031], P<0.05). Conclusion: CXB at 3, 10 mg·kg -1·d-1 can reduce inflammatory cell infiltration and inhibit the proliferation of Han: SPRD rat's renal cyst, probably through inhibition of COX-2.

Objective: To investigate whether celecoxib (CXB), a specific COX-2 inhibitor, can inhibit the proliferation of cyst lining epithelial cells through blocking mitogen-activated protein kinase (MAPK) signal transduction pathway. Methods: Primarily cultured cells were treated wi th different concentrations of CXB (0,2.5×10-6, 5×10-6, 1×10-5, 2×10-5, 3×10-5, 4×10-5, 5×10-5 mol/L) and the proliferative status was evaluated by BrdU assay. The levels of vascular endothelial growth factor (VEGF) were measured by enzyme-linked immunosorbent assay (ELISA); the production of proliferating cell nuclear antigen (PCNA) and phospho-MAPK were measured by real-time reverse transcription-PCR assay; and the expression of PCNA, MAPK and phospho-MAPK protein was detected by Western blotting. Results: BrdU as say revealed that CXB inhibited cell growth in a concentration-dependent manner; the maximum inhibition rate ([63.9±1.2]%) was found when cells were treated with 2×10-5 mol/L CXB for 24 h. VEGF secretion by cyst lining epithelial cell was reduced by CXB in a concentration and time-dependent manner. The mRNA and protein levels of PCNA, phospho-MAPK in CXB-treated group were lower than those in control group (with no CXB treatment). Conclusion: CXB ca n obviously inhibit the proliferation of cyst lining epithelial cell and the secretion of VEGF, which might be through interfering with the phosphorylation of MAPK and partly blocking MAPK signal transduction pathway.

Objective: To explore the mechanism of neuroprotective effects of aspirin after cerebral ischemia/reperfusion injury in rats. Methods: Cerebral ischemia/reperfusion model of middle cerebral artery occlusion was established in rats using the suture method. Forty-eight experimental rats were divided into control group (group A), low-dose group (group B; aspirin 20 mg/kg), moderate-dose group (Group C; aspirin 80 mg/kg), and high-dose group (group D; aspirin 320 mg/kg), each group had 12 rats. Aspirin were continuously injected intraperitoneally in the rats in experimental groups for 3 days as an interference factor after cerebral ischemia/reperfusion. At the same day after cerebral ischemia/ reperfusion, the neurological function of all the rats in each group was evaluated for 4 days by the Bederson score and was documented. All the rats in each group were killed at day 4. Cerebral infarct volume were measured by TTC stain, the apoptosis in situ labeling were detected by TUNEL, and the expressions of apoptosis-regulating proteins BCL-2 and BAX were detected by inummohistochemistry. Results: Limb function improved in the groups of A, B, and C after aspirin intervention. As compared with the group A, the neurological deficit scores decreased significantly, and the infarct volume reduced significantly (compared with the group A, the infarct volume decreased 16.3%, 19.2%, and 12.8%, respectively); the positive rate of apoptosis cells in the surrounding ischemic areas decreased, they were 58%, 37%, 35%, and 40% in groups A, B, C, and D; the protein Bcl-2 expression in the ischemic areas increased 38%, 55%, 60%, and 50% in groups A, B, C, and D; and protein Bax expression decreased 50%, 34%, 33%, and 42% in groups A, B, C, and D. Among the 3 treatment groups, the efficacy of the low-and moderate-dose groups was better than that of the high-dose group. Conclusion: Early use of aspirin may decrease neurological defic its after cerebral ischemia/reperfusion in rats, and thus exert its neuroprotective effect.

Mycoplasma pneumoniae encephalitis is one of the extrapulmonary complications of mycoplasma pneumoniae infectiontion children.The clinical diagnosis of mycoplasma pneumoniae encephalitis is difficult, so it is necessary to make stratified diagnosis based on the diagnosis of encephalopathy and the reliability of pathogen detection evidence.In addition to considering whether antibiotics are sensitive to mycoplasma pneumoniae, the selection of antibiotics should also consider the tissue penetration of antibiotics and the safety of children′s medication.These problems may lead to the delay in diagnosis as well as treatment of mycoplasma pneumoniae encephalitis in children and affect the prognosis of children.This paper intended to introduce and summarize the diagnosis of mycoplasma pneumoniae encephalitis and the selection principles and characteristics of main antibiotics for mycoplasma pneumoniae encephalitis, hoping to be helpful to clinicians.

Objective To evaluate the localization and the correlation with cardiovascular risk factors of cerebral arteries damage in patients with transient ischemic attack (TIA). Methods Digital subtraction angiography (DSA) was performed in the 97 patients with TIA. The localization, the degree of stenosis, the numbers of the arteries involved and their correlation with the cardiovascular risk factors were analyzed. Results DSA showed occlusive findings of cerebral arteries in 91.8% of the patients, and 68 cases (76.4%) among them were involved more than one artery. The proximal internal carotid arteries (35.5%) and the proximal vertebral arteries (28.2%) were the most frequently involved in this series. And the number of the arteries involved and the degree of stenosis were positively correlated with the level of risk factors. Conclusion There is a high incidence of cerebrovascular stenosis in patients with TIA. Most of them are involved more than one artery. The degree of the stenosis is positively related with the quantity of the risk factors.

Objective To investigate the Lemple-Zie complexity (LZC) characteristics in patients with Alzheimer' s disease(AD),mild cognitive impairment(MCI) and normal elderly,and the possibility of differentiating AD,MCI and normal elderly by LZC.Methods Electroencephalogram (EEG) of 30 AD patients,30 MCI patients and 20 normal elderly with eyes closed in rest state were recorded.In acquired EEG data,2048 points(10.14 s)of each channel were selected for LZC calculation by Matlab 7.0 software.Results (1) The average LZC values in AD,MCI and control groups were 0.396 ± 0.036,0.470 ±0.051,0.523 ±0.055 respectively.As compared with control group,the values in AD and MCI groups were decreased (F =43.092,P =0.000).(2) LZC values of AD in all channels (from 0.373 ± 0.042 to 0.430 ±0.083),whole brain,bilateral hemispheres,frontal and temporal areas were significantly lower than those in the normal group (from 0.498 ± 0.067 to 0.566 ± 0.059 ; t =3.602-8.747,P =0.000-0.010),and showed the significant decline(＞ 23％)in bilateral anterior middle temporal,frontal areas,the left parietal area.(3) Except the T6 channel,LZC values of AD groups in the remaining channels(from 0.373 ±0.042 to 0.418±0.063),the whole brain,bilateral hemispheres,frontal and temporal areas were significantly lower than those in the MCI group(from 0.455 ± 0.072 to 0.489 ± 0.063 ; in T5 channel,t =2.038,P =0.041,the others t=4.178-7.424,all P=0.000).(4) LZC values of MCI groups in the whole brain,bilateral hemispheres,temporal areas,parietal areas,the left frontal area were notably lower than those in the control group.Conclusions With the decline of the cognitive function,the EEG complexity value shows the parallel change.It suggests that the EEG complexity value can reflect the change of brain function in the duration from normal age to dementia in some degree; The MCI patients with abnormal LZC values in temporal and frontal lobes have a certain degree relation with the occurrence of AD.

This paper probes into the scientific research management at a teaching hospital,where three responsibilities are required in clinical service,medical education and research.The staffs＇ need for scientific research is discussed base on Maslow＇s need-hierarchy theory.An incentive system is also proposed for research and professional development of the hospital.

Objective To compare the effects of transcranial and trans-spinal low frequency magnetic stimulation (MS) on the expression of growth-associated protein-43 (GAP-43) in the spinal neurons of rats after spinal cord injury (SCI). Methods Forty-five Sprague-Dawley female rats were divided randomly into a transcranial magnetic stimulation (TMS) group,a trans-spinal cord magnetic stimulation group and a model control group with 15 in each group.Another 6 rats served as a normal control group.Spinal cord injury at the T9-10 level was modeled according to the modified Allen's method.The rats in the two MS groups received magnetic stimulation at 0.5 Hz and 75％ of the maximum output intensity ( 1.9 T) of the magnetic stimulator beginning 24 h post injury.They received 30 pulses daily for 28 days while the rats in the model control group were not exposed to MS.Basso-Beattie-Bresnahan (BBB) scale scores and motion evoked potentials (MEPs) were measured at the 1st,7th,14th,21 st and 28th day post injury.The expression of GAP-43 protein was studied using immunohistochemical methods and semi-quantified by Western blotting. Results At the 4th week post injury the average BBB scores in the model control group,the TMS group and the trans-spinal cord magnetic stimulation group were all significantly different. MEP amplitudes decreased significantly in all groups after the injury,and the latency was prolonged,but there were no significant differences among groups at any time point.GAP-43 expression also increased after SCI,and it was significantly higher in the MS groups than in the model control group at 4 weeks. Moreover,the expression of GAP-43 in the trans-spinal cord MS group was significantly greater than in the TMS group.Conclusion Magnetic stimulation can promote nerve regeneration and improve locomotor recovery after spinal cord injury,at least in rats.The effect of trans-spinal cord magnetic stimulation is superior to that of TMS.The mechanism may be related to the activation of central pattern generators.