Objective　To study the ultrasound image feature of acute hepatic lesions in different times.Methods　The feature of ultrasonography in 126 cases with acute hepatic lesions were analyzed and divided into two groups according to different fime after injury: group A, 25～60 minutes after injury; group B, 60～120 minutes after injury.Results　There was significant difference in the structure of sonographic classification between two groups. The high echos was obviously higher in group A than that in group B, while the mixed echos was obviously higher in group B than that in group A (P<0.001). Compared each types in group A and in group B,the high achos in group A and the mixed achos in group B were higher than other types in same group (P<0.001).Conclusions　The ultrasonographic feature of acute hepatic lesions would change with time. The high echos occurs in eaily time after injury ,and then changes into the mixed echos.

Objective To explore the influences of parent-child attachment security memory activation on college students'general attachment working model. Methods 152 college students were randomly distributed to three groups, among which there were two experimental groups accepting father-child or mother-child memory activation,and one control group accepting non-interpersonal memory activation. Results Mather-child security memory activation significantly increased subjects' scores on others-model of general attachment to females (F= 3.83;P ＜0.05), scores of mather-child security memory activation group were significantly higher than those of fatherchild group ( 7.88 ± 7.24,4.49 ± 7.53; P ＜ 0.05 ) and control group ( 7.88 ± 7.24,4.71 ± 6.83; P ＜ 0.05 ).Father-child security memory activation significantly increased subjects' scores on self-model of general attachment to males (F= 6.35; P ＜ 0.01 ), scores of father-child security memory activation group were significantly higher than that of mather-child group (6.33 ±5.39,2.44 ±6.14; P＜0.01 ) and control group (6.33 ± 5.39,2.59 ±6.10; P ＜ 0.01 ). Conclusion Parent-child attachment security memory activation have major influences on college students' general attachment working model, in which the effect of gender differences provides some inspiration for clinical interferences.

BACKGROUND: Lumbar spondylolisthesis directly reduces contact areas between dislocated vertebral body and subjacent vertebral body, which is an important factor that decides intervertebral stress and lumbar degeneration. The cross section of lumbar is irregular reniform shaped and there is no mathematical formula to calculate changing regularity of intervertebral contact areas of lumbar spondylolisthesis directly.OBJECTIVE: To study changing regularity of intervertebral contact areas during lumbar spondylolisthesis and to analyze its clinical significance. METHODS: Super-surface of L5 vertebra and sub-surface of L4 from 25 cases were taken by a digital camera and computer simulation spondylolisthesis process and every intervertebral contact areas (Sn) were measured by Image-Pro Plus software. The mean value was obtained and converted into percentage area according to Sn%=Sn/S×100%. The change rules of vertebral bodies were observed from 0 to 100% spondylolisthesis. Based on this regularity, a new clinical stage of lumbar spondylolisthesis was proposed and guided for treatment of 56 cases with lumbar spondylolisthesis. RESULTS AND CONCLUSION: During lumbar spondylolisthesis process, Sn% changes like a hyperbola. Sn% lost slowly during spondylolisthesis rate at 0-23% and quickly during 23%-44%, and then it become slowly once again after spondylolisthesis rate at 44%-100%, the inflection points appeared at (23±2)% and (44±2)%. Totally 48 patients were followed up, according to Staufee standard rate, the clinical curative effect reached approximately 90%. It suggests that the intervertebral contact areas present with a non-linear change, which is helpful to judge the stability of lumbar spine and guide the treatment of lumbar spondylolisthesis.

BACKGROUND: Soluble dispersive mixture of domestic chicken collagen type Ⅱ (SmCC Ⅱ ) has been proven to be safe and effective for rheumatoid arthritis (RA) treatment while there are some similar articular cartilage degradation and autoimmune pathogenesis between osteoarthritis (OA) and RA, so it is worth studying whether SmCC Ⅱ is effective for the precaution or treatment of OA.OBJECTIVE: To observe the prophylactic and therapeutic effects of SmCC Ⅱ on rat OA and analyze the concomitant matrix metalloproteinase (MMPs) and tissue protease changes in osteoarthritic rats.DESIGN: Randomized and controlled observation.SETTING: Department of Clinical Pharmacy, Sixth People's Hospital, Shanghai Jiao Tong University.MATERIALS: Totally 258 rats of clean grade and either gender, aged 6 weeks were involved in this experiment. SmCC Ⅱ (white powder, Batch No.00031004) was provided by Professor Ren Geng-Fu from Shanghai Engineering Institute of Herbal Biomedicine. The 20 mg/L and 80 mg/L solution of SmCC Ⅱ effective component were prepared before use.While 0.25% excipient (mannitol, product of Jiangsu Tianyuan Medical Co., Ltd) solution was prepared by 200 g/L mannitol dissolved in sterile saline solution.METHODS: The study was carried out in the Whole Animal Laboratory and Experimental Center, Sixth People's Hospital,Shanghai Jiao Tong University between March 2003 and February 2006. ①Prophylactic study: Totally 132 rats were randomized into 5 groups: OA group (n =36): treated with sterile saline solution orally by a 16G syringe, 1mL/d; Low- and high-dose SmCC Ⅱ groups (n =24, respectively): treated with 20 mg/L and 80 mg/L SmCC Ⅱ solution orally, 1 mL/d;Excipient group (n =24): treated with 2.5g/L mannitol, 1 mL/d. OA models were surgically induced in these 4 groups by Hilth's method; Normal group (n =24): Rats without operation were treated with sterile saline solution orally, 1 mL/d. All the rats were administrated on the day of modeling. ②Therapeutic study: Totally 126 rats were randomized into 5 groups, grouping, administration and rat number in different groups were the same as those in prophylactic study,respectively, except for the n =30 in OA group. In addition, all the rats were adminstrated for 8 weeks since 6 weeks after operation. The knee joints of right hind limb of all the rats were taken off after 1-week treatment. And sample was cut open along coronal incision for use. ③Morphological study of articular cartilage was conducted by HE staining and Mankin score was calculated to evaluate the severity of arthritis, immunohistochemical studies of matrix metalloproteinase (MMP)-13, MMP-9 and cathepsin K (Cath K) were carried out by ABC method in situ and the percentage of positive-stained chondrocytes were calculated while the mRNA level for MMP-13, MMP-9, Cath K and the tissue inhibitor of metalloproteinase 1 (TIMP1) were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) method.MAIN OUTCOME MEASURES: ①The morphological changes of articular cartilage in different groups in prophylactic or therapeutic study.②The level of MMP-13, MMP-9, Cath K and their corresponding mRNA levels.RESULTS: All the 258 rats were involved in the final analysis. ①Prophylactic study: Apparent degeneration appeared in the rats of OA group and the mankin score in OA group was higher than that in high- or low- SmCC Ⅱ groups [(6.44±0.81), (2.75±0.85), (2.70±0.81) points respectively, P ＜ 0.05], the mRNA and positive-stained chondrocyte percentage of MMP-13, MMP-9 and Cath K in OA group was higher than that in high- or low-dose SmCC Ⅱ group, respectively (P ＜0.05-0.01) while the TIMP1mRNA level in OA group was not significantly higher than that in high or low SmCC Ⅱ group (P＞ 0.05). There were no significant changes on the level of MMP-13, MMP-9 and Cath K between excipient and OA group (P ＞ 0.05). ②Therapeutic study: The Mankin's score of OA group was higher than high or low SmCC Ⅱ group [(6.96±1.02), (3.08±0.45), (4.00±0.94) respectively, P ＜ 0.05-0.01] while the mRNA and positive-stained chondrocyte percentage of MMP-13, MMP-9 and Cath K in OA group was higher than that in high- or low-dose SmCC Ⅱ group,respectively (P ＜ 0.05-0.01) while the TIMP1mRNA level in OA group was not significantly higher than that in high- or low-dose SmCC Ⅱ group (P ＞ 0.05).CONCLUSION: SmCC Ⅱ can delay the degradation of articular cartilage of OA rats and maybe effective for OA prevention or treatment. The mechanism maybe related to SmCC Ⅱ reducing the synthesis of MMP-13, MMP-9 and Cath K at transcriptional level.

Objective To investigate the influence of Zafirlukast on the inflammatory factors and immune function of patients with seasonal allergic asthma.Methods 182 patients with seasonal allergic asthma from the department of respiratory in our hospital were selected and divided into two groups, 91 cases in each group.The control group treated with budesonide and formoterol fumarate powder for inhalation, the experiment group received Zafirlukast on the basis of the control group,serum levels of inflammatory cytokines,peripheral blood T lymphocyte subsets,serum immunoglobulin levels, lung function,and the clinical effect were compared after the treatment.Results Compared with the control group, serum levels of TNF-,IL-4,IL-6 were lower, level of IL-10 was higher in the experiment group after treatment, CD8 +T lymphocyte levels was lower, CD3 +, CD4 +T lymphocyte and CD4 +/CD8 +ratio levels were higher after treatment, serum levels of IgE, IgG, IgA were lower after treatment, levels of PEF,FEV1 ,FEV1/FVC were higher after treatment, the difference was statistically significant (P<0.05).The effective rate in the experiment group 94.51% was higher than the control group 81.32% (P<0.05).Conclusion Zafirlukast can significantly reduce the levels of serum inflammatory factors in patients with seasonal allergic asthma, improve the cellular immune function, reduce the level of immunoglobulin, effectively improve lung function, shorten the length of hospital stay, has better clinical curative effect.

Based on the demand analysis,the paper designs and develops the direct network report system for the comprehensive statistical management platform of Traditional Chinese Medicine (TCM) and introduces the specific implementation and application of the system.This direct report system conforms to the work requirements of TCM statistics,optimizes the process of data reporting,has complete functions and enhances the work efficiency of statistical departments.

0.05). Conclusion The present method has many advantages such as simplicity of operation,rapidity,better accuracy for the accurate quantitative analysis of urinary manganese.

Objective To determine the relationship between endothelin-l(ET-1)with blood flow of gastric mucosa and gastric mucosal injury (GMI) in obstructive jaundice rats induced by coldly restrainting stress. Methods 161 rats were randomly assigned into the experimental (obstructive jaundice) group (n=70), the control (sham operative) group (n=70) and the anti-ET-1 preconditioning group (n=21). The obstructive jaundice model was set up in the experimental group and the anti-ET-1 preconditioning group. Both the experimental group and the control group were re-assigned into 5 subgroups which underwent non-coldly restraint and coldly restraint for 10, 30, 60 and 120 min respectively. The anti-ET-1 preconditioning group underwent anti-ET-1 serum precondition 30,60 or 120min before the coldly restraint. The ET-1 was detected by radioimmunologic method and the gastric mucosal blood flow (GMBF) by laser Doppler flowmeter. Results The concentrations of ET-1 in gastric mucosa and ulcer index significantly increased (P

Objective To investigate the risk factors associated with early mortality of the rapid two-stage arerial switch operation, which has a significantly higher overall mortality than that of ASO procedure for D-TGA with a intact ventricular septun. Methods The data we reviewed involving patients who underwent rapid two-stage switch operations from September,2002 to September, 2007 in our center, 13 patients were male and 8 were female, their age at operation ranged from 29 to 250 days afer birth, and the body weight was 3.5 to 7 kg. Chi-squared test and multivariant logistic regression methods were used for the analysis of demographic data, pre-diagnosis information, operation data, interval data combined with the operation time,left ventricle training condition, and the early post-operative outcomes. Results The operative mortality was high at initial stage, and then decreased gradually. The logistic multivariant regression analysis indicated that the mortality of left ventricular training operation was associated with the diameters of BT shunt ( P =0.003 ); the mortality of two-stage switch was associated with feminie ( P = 0.006 ) and pre-operative p. LV/RV ( P ＜ 0.001 ). Conclusion Patients with transposition of the great arteries and intact ventricular septum who missed the optimal time for switch operation should receive rapid two-stage switch operations, which provide an opportunity for the correction of the deformity. The key factor associated with the success for operation was good in heart function after left ventricular training.

The non-invasive positive-pressure ventilation(NPPV) play an important role in treating patient with upper airway obstructive diseases.The use of NPPV in children has significantly increased in recent years internationally.Nevertheless,the paediatric NPPV support in China was less reported.This review aimed to clarify the indications,efficacy and technical methods in NPPV,and therefore searched papers including clinical studies,reviews and guidelines focusing on children with upper airway obstructive diseases who were treated on NPPV.The review concluded that NPPV is a treatment choice for patient who has upper airway obstruction and can not be solved by surgery.The NPPV avoids the impairment to the body caused by recurrent hypoxemia and arousals,decreases the rate of intubation and improves the quality of life.NPPV need to be preformed in a professional children's medical center.Education and training for the parent is the key point of improving treatment compliance.